• Applied Microscopy
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• 제23권1호
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• pp.91-108
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• 1993

The relationship of cartilage canals to initial osteogenesis of primary ossification center of developing vertebrae in human fetuses ranging from 50mm to 260mm in crown rump length was studied by light and electron microscopy. The cartiage canals of the thoracic vertebrae were first observed at 60mm fetus. Cartilage canals were identified as vascular channels arising from perichondrium surfaces. A number of cartilage canals were observed around the primary center of ossification at 80mm fetus. At 120mm fetus, cartilage canals of the bodies of vertebra were increased. Eventually the canals were eroded from the main medullary cavity and remained at only peripheral regions of growth cartilage. Superficial, intermediate, and deep canals were identified by the characteristics of cartilage cells. Fibroblasts, undifferentiated mesenchymal cells, and vacuolated macrophages were observed adjacent to the matrix of resting cartilage cells in the superficial canal. Fibroblasts and mesenchymal cells were densely packed at the tip of canal, giving an epithelial appearance to the clustered cell in the intermediate canal. Vacuolated macrophages were in contact with matrix of hypertrophied cartilage. The thick-walled vessels in the intermediate and deep canals consisted of typical endothelial cells, but in the newly formed vessels contained mesenchymal cells and fibroblasts incorporated into the vessel wall. During lengthening of cartilage canal, the matrix of cartilage cells were invaded by newly formed capillaries and vacuolated macrophages. At the deep canal, the lateral wall of the canal terminated in matrix containing calcified cartilage. The mesenchymal cells began to differentiate into osteoblasts adjacent to the calcified matrix. The results indicate that the connective tissue cells within the cartilage canals proliferate and differentiate into osteoblasts at the site of primary ossification center.

• 대한수의학회지
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• 제37권2호
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• pp.269-280
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• 1997

The development and change of the cartilage canals ralated to chondrocyte growth and the major histological pattern were investigated in the calcaneus of the Korean native cattle fetuses from CRL 28mm to 175mm. The results as follows; 1. The cartilage canal first appeared in the caudal subperichondral region of the sustentaculum tali of the CRL 28mm fetus, and well developed after the period. 2. The three major cartilage canals, one entered from the head of calcaneous into where achilles tendon insert and the others from the caudal region of sustentaculum tali and the calcaneal sulcus, growth distinctively. The latters participated in the ossification center prior to the former. 3. The number of cells in this canals and the size increased synchronously with chondrocytic hypertrophy. In the intermediate and deep canals, the chondrogenic change showed distinctinvely. Our results demonstrated that the cartilage canals from the lower part were important the formation of the ossification center, and the growth and development of canals associated with the hypertropic changes of chondrocytes in the calcaneus of the Korean native cattle fetus.

• 대한기관식도과학회:학술대회논문집
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• 대한기관식도과학회 1991년도 제25차 학술대회 연제순서 및 초록
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• pp.13-13
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• 1991

후두는 호흡기가 시작되는 부위로 후두의 태생학적 연구는 타장기에서와 같이 정상 후두의 해부학적 이해, 후두 기형의 병인의 이해 및 치료에 기초 자료가 된다. 그러나 사람 배아의 후두 발생에 관한 연구는 재료확보가 용이하지 않고 해부학적 난이성으로 매우 드물다. 이에 저자는 사람 배아의 후두 발달을 연구하고자 배령이 확인된 20례의 배아(배령 4 주에서 8 주 까지)의 연속절편에서 광학현미경하에 후두 발달의 형태학적 관찰을 시행하여 다음과 같은 결과를 얻었다. 1. 배령 4 주에 median pharyngeal groove, laryngotracheal sulcus 와 tracheoesophageal septum 이 관찰되었다. 2. 배령 5 주에 hypopharyngeal eminence, epithelial lamina of larynx와 arytenoid swelling이 관찰되었다. 3. 배령 6 주에 hyoid condensation과 tracheoesophagealfistula가 관찰되었다. 4. 배령 7 주에 epiglottis 가 확인되었고 hyoid bone, thyroid lamina 와 cricoid cartilage 의 condensation 및 muscular condensation 이 관찰되었다. 5. 배령 8 주에 laryngeal cartilage의 chondrification과 ventricle이 관찰되었으며, vestibulotracheal canal과 pharyngotracheal canal 의 교통은 관찰되지 않았다.

• 한국임상수의학회지
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• 제21권2호
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• pp.149-153
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• 2004

This study was to determine the procedure for ultrasonographic imaging of the canine external ear canal(ECC). By infusion of warm saline solution into the ear canal, a real-time B mode ultrasonographic examination of the ECC was performed in five healthy Beagle dogs and two dogs with severe otitis externa. The ultrasonography was very useful to detect the structures of the ECC and the tympanic membrane. An 11 NHz linear probe was permitted to assess an anechoic lumen of the ECC and a hyperechoic cartilage surrounding the ear canal. In two dogs with otitis externa, it could be assessed the ECC with ultrasonography without ear cleansing. A lumen of the ECC was irregular and a cerumen could be seen, but edematous inflammatory changes of the ECC could not be identified. The results suggest that ultrasonography of the ECC with an 11 NHz linear probe after infusing the saline solution into the ear canal may be an accurate, non-invasive, rapid, and widely available method for assessment of the ear canal diseases such as otitis externa, polyp, stenosis, hyperplasia, and foreign body in dogs.

• Applied Microscopy
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• 제31권3호
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• pp.287-305
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• 2001

태생기 성장골에 출현하는 연골관은 골화와 무관하고 영양분을 공급하는 통로라 하였고 일부 학자들은 연골관 말단부에서 골화가 일어남을 발표하였다. 추체에서 연골관은 골화중심부 출현이전에 나타나 전 태생기동안 존재하기 때문에 연골관과 골화와의 관계를 관찰하는 것은 골화과정을 이해하는데 의의 있는 일이라 사료된다. 본 연구는 연골관이 추체연골부 골화중심부 출현에서부터 골화가 진행하는 과정에 어떠한 역할을 하는가를 전자현미경으로 관찰하여 새로운 지견을 얻었기에 그 결과를 보고하고자 한다. 좌고 60mm(태령 12주)때 추체에 연골관이 출현하기 시작하였고, 80mm(13주)때 추체 중앙부에는 석회화연골세포와 비대연골세포로 구성된 석회화소가 출현하였으며 심연골관이 비대연골세포대에서 관찰되었다. 비대연골세포의 형태는 다양하였고 불규칙하게 배열하고 있었다. 심연골관의 끝부분에는 골형성세포, 골모세포 및 파연골세포들이 관찰되었고 이것이 1차 골화중심부 출현이었다. 비석회화 연골기질은 연골관내에 존재한 혈관주위 결합조직들에 의해 주로 흡수되었고 파연골세포에의한 석회화 연골기질의 흡수는 활발하지 많음을 볼 수 있었다. 120mm(16주)때 추체 연골내골화가 전 후방으로 진행되었고 전 후방 연골막에서 막내골화가 시작되어 막성골층판이 신생되었다. 결론적으로 추체의 골화과정은 장골의 골단연골의 골화과정과 유사하였고, 특히 추체에 출현하는 연골관의 주위에 존재하는 결합조직성 세포들이 골형성세포 및 골모세포로 분화 발육하여 신생골이 형성됨을 알 수 있었다.

• 한국임상수의학회지
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• 제36권5호
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• pp.266-270
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• 2019

A 13-year-old spayed female American cocker spaniel dog was submitted to an animal hospital with a mass from left ear canal and enlarged left parotid lymph node. Given one-year history of odorous exudates and chronic otitis externa, total ear canal ablation was performed. Grossly, because of the neoplastic mass in both inner and outside of annular cartilage in external ear, vertical ear canal was severely narrowed. Histologically, there were numerous proliferated glands in the ear canal mass. Many neoplastic glands contained secretory vesicles on the free margin and necrotic cellular debris. Severe multifocal necrosis and strong invasion were also observed throughout the mass. Massive metastatic foci of glandular structures originated from ceruminous gland were presented in the enlarged parotid lymph node. Neoplastic glandular epithelia contained PAS-positive diastase-resistant eosinophilic cytoplasmic granules. Immunohistochemically, the neoplastic cells showed positive reactions for cytokeratin (CK) 7 and negative for CK 5/6. Based on the clinical implication and gross findings, histopathology and immunohistochemistry, this case was diagnosed as metastatic ceruminous gland adenocarcinoma in the American cocker spaniel.

• 한국임상수의학회지
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• 제21권2호
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• pp.97-101
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• 2004

This study was aimed for ultrasonographic assessment of the tympanic membrane and the tympanic bulla in five healthy Beagle dogs. To improve an ultrasonographic image, the ear canal was filled with warm saline, and an 11 MHz linear probe and a 6.5 MHz convex probe were used. The structures of ear component such as ear canal, ear cartilage and tympanic membrane were easily identified. Especially, tympanic membrane was presented as a reflaction surface which was resulted from the different acoustic impedence between the fluid-filled anechoic ear canal and the gas-filled hyperechoic tympanic cavity in normal dogs. In five left-side ears, the saline was infused into the external ear canal after the tympanic membrane had been ruptured experimentally. Both anechoic fluid-filled ear canal and tympanic cavity were clearly identified. In five right-side ears, the surgically fluid-filled tympanic cavity was imaged as a hypoechoic oval shaped structure. When tympanic cavity and ear canal have been contained with fluid, it was difficult to identify whether the tympanic membrane was ruptured or not. For assessment of the ear structure with ultrasonography, the 11 MHz linear probe was considered as an optimal equipment for a serial assessment of ear canal, tympanic membrane and tympanic bulla whereas the 6.5 MHz convex probe was suitable to assess the tympanic cavity. The results suggest that ultrasonography with saline infusion into the ear canal can be used to find the intactness of the tympanic membrane and to assess the fluid- filld tympanic bulla.

• 한국임상수의학회지
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• 제15권2호
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• pp.263-266
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• 1998

외이도의 직경 및 길이와 외이도 질환과의 연관성을 알아보고자 73두 성견을 실 험에 이웅하였다. 임상중상, 배양 및 도말 검사를 통하여 외이 질환이 얼는 정상군 57개의 이 관과, 질환이 있는 비정상군 89개의 이관에 직경과 길이를 평가하기 위한 간접적인 방법으로 이관내 조영술을 실시하였다. 비정상군은 정상군에 비하여 윤상연골 및 귓바퀴 연골의 직경 이 더 넓었다. 수직외이도를 구성하는 귓바퀴 연골의 길이는 비정상군4(12.79{\pm} 3.08)이 정상 군(12.79{\pm}5.87 mm)$에서 보다 유의성 있게 길게 나타났다(p<0.001). 심한 외이도 협착증을 보이는 21개의 귀에서는 외이도 질환을 발견할 수 없었으며, 외이도 질환이 심한 귀에 있어 서 그 직경과 길이가 정상군에 비하여 넝고 길게 나타났다(p<0.05). 본 실험의 결과로 보아 외이도관의 협착증과 외이도 질환과는 연관성이 발견되지 않았으나, 환기와 귀내 분비물의 배출을 방해하는 긴 수직외이도를 갖는 귀가 외이도 질환과 밀접한 관계가 있는 것으로 생각 되었다.

• Archives of Plastic Surgery
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• 제37권6호
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• pp.827-830
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• 2010

Purpose: Congenital spinal dermal sinus tract is a rare lesion connecting skin to deeper structures including neural tissue. It results from the failure of the neuroectoderm to separate from the cutaneous ectoderm in the third to fifth week of gestation. The common locations are the lumbosacral and occipital regions. Sometimes it extends to spinal canal. In this paper we report a case of congenital spinal dermal sinus tract in the coccyx. Methods: A 21-month-old male child born after an uncomplicated full-term pregnancy was admitted to our institute with a midline dermal sinus and a cartilaginous protrusion in the coccygeal region. There were no signs of infection. Neurologic examination showed no functional deficit in both lower limbs. He was treated with complete excision of the tract and an underlying accessory cartilage. Results: The spinal dermal sinus tract was extended from the skin to the coccyx. The stalk was loosely attached to the accessory cartilage of coccyx. At that point, it was dissected from the accessory cartilage and resected. The accessory cartilage was also resected at the bone and cartilage junction. During the follow-up period of 6 months, the wound healed well without any complication nor recurrence. Conclusion: Congenital spinal dermal sinus tract is known as a form of spinal dysraphism. In order to prevent complications, timely surgical intervention including complete resection of sinus tract with correction of associated abnormalities is of utmost importance.

• Journal of Audiology & Otology
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• 제25권2호
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• pp.104-109
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• 2021

Background and Objectives: To assess whether the audiological and clinical outcomes of type 0 tympanoplasty (T0) performed using cartilage were comparable with those of ossiculoplasty in patients who underwent canal wall down mastoidectomy (CWDM). Subjects and Methods: This study included patients who had chronic otitis media with cholesteatoma and underwent CWDM with ossiculoplasty involving partial ossicular replacement prosthesis (PORP), total ossicular replacement prosthesis (TORP), or T0. Anatomical success rates and hearing outcomes were analyzed. Results: Seventy-two patients were included in this study; 29 of them underwent CWDM with T0, 27 underwent CWDM with PORP, while 16 underwent CWDM with TORP. The difference in mean improvement in the air-bone gap (ABG) between the groups was not significant. The differences in the rates of ABG closure to ≤10 dB HL (p=0.030) and ≤20 dB HL (p=0.029) were significant. There were significant differences in improvements in the ABG at 3 kHz among the PORP, TORP, and T0 groups. Conclusions: The audiological outcomes of CWDM with ossiculoplasty seemed to be better than those of CWDM with T0 with no significant difference in the incidence of complications following ossiculoplasty and T0.