• 대한소아치과학회지
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• 제27권1호
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• pp.169-179
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• 2000

초기 우식병소와 건전한 치질은 광학적 특성 이 다르다는 사실에 근거하여 치과 치료용으로 개발된 가시광선 영역의 아르곤 레이저의 형광현상, 즉 물체에 조사되어 반사된 및은 물체의 광학적 특성에 따라 파장이 달라지는 특성을 이용하여, 우식 치질과 건전한 치질간의 광학적 특성의 차이를 아르곤 레이저 빛을 통한 색조의 차이로 유도 전환하는 시스템을 개발하고 이를 이용하여 초기 치아우식 병소를 조기에 시각적으로 탐지하여 data화 하는 새로운 우식활성검사법을 개발하는데 본 연구의 목적이 있다. 연구는 3단계로 나누어 1단계는 아르곤 레이저 형광법의 초기 우식병소 탐지능력을 평가하였으며 2단계는 임상에서 아동들을 대상으로 아르곤 레이저 형광법을 이용하여 초기 우식병소가 있는 치아의 갯수를 집계하고 이 수치에 대한 우식활성검사법인 $Cariescreen^{(R)}$ test, 그리고 일종의 구강환경검사인 우식경험치아의 수(dDfFtT) 검사와의 상관성을 평가하였다. 3단계는 이와 같은 자료를 기준으로 하여 레이저 형광법을 이용하여 검사할 수 있는 새로운 우식활성검사 모델을 만들고 이와 같은 검사법의 민감도, 타당도, 진단력 등을 분석하여 진단학적 측면에서 활용 가능성을 평가하고자 하였으며 그 결과는 다음과 같다. 초기 우식병소의 조직학적 깊이가 증가될수록 레이저 형광법을 이용하여 측정한 광밀도가 증가하였으며 이들 사이에는 높은 상관관계 (=0.7015)가 있었다. 또한 통계학적으로 회귀분석을 시행한 결과 병소의 깊이와 광밀도와 사이에는 정비례 관계의 직선방정식 관계를 나타내었다. 레이저 형광법을 이용한 우식활성검사법은 $Cariescreen^{(R)}$ test 그리고 dDfFtT 검사 등과 높은 상관관계를 나타냈다. 또한 레이저 형광법을 이용한 우식활성 검사법은 dDfFtT검사를 기준으로 48%의 민감도, 52%의 특이도, 45%의 진단력을 보였으며 $Cariescreen^{(R)}$ test를 기준으로 48%의 민감도, 51%의 특이도, 36%의 진단력을 보였다. 이상의 결과를 종합하면 레이저 형광법을 이용하여 측정한 초기 우식병소의 광밀도는 실제 초기우식병소의 조직학적병소의 깊이를 평가하는데 높은 진단학적 신뢰성을 보여주고 있으며 레이저 형광법을 이용한 우식활성검사는 진단학적 지표를 기준으로 볼 때, 그리고 검사절차와 시간, 비용 등의 면으로 볼 때 향후 임상적으로 활용 가능성이 높은 것으로 사료된다.

• 한국치위생학회지
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• 제12권1호
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• pp.81-91
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• 2012

Objectives : By investigating antimicrobial activity of natural extracts, identifying its usefulness as antibiotic material to oral bacteria. Methods : Antimicrobial activity tests of 25 natural extracts were implemented on 6 type strains alleged to cause dental caries and 10 clinical strains isolated and identified from dental caries. Results : Among medicinal plants, Coptis japonica, Scutellaria baicalensis, Dictamnus dasycarpus showed antimicrobial activity. Among lichens, 3 methanol extracts and 6 acetone extracts showed antimicrobial activity. Minimum Inhibitory Concentration(MIC) test of Usnea aurantiacoatra KoLRI 004184, an acetone extract of lichen with the highest antimicrobial activity, on 6 type strains involved in dental caries and 10 strains isolated from dental caries resulted as follows; Actinomyces oris 1041 $12{\mu}g/ml$, for Corynebacterium durum 3151 $13{\mu}g/ml$, for Rothia dentocariosa KCTC $3204^T$ $14{\mu}g/ml$, for R. dentocariosa 911 $15{\mu}g/ml$, for R. dentocariosa 1822 $12{\mu}g/ml$, for Lacto-bacillus casei KACC $12413^T$ $12{\mu}g/ml$, for L. acidophilus KACC $12419^T$ $16{\mu}g/ml$, for L. rhamnosus 2421 $15{\mu}g/ml$, for Streptococcus mutans KCTC $3065^T$ $15{\mu}g/ml$, for S. mutans 121 $13{\mu}g/ml$, for Streptococcus oralis 2221 $18{\mu}g/ml$, for S. salivarius KCTC 5512 $14{\mu}g/ml$, for S. salivarius 122 $12{\mu}g/ml$, for S. sanguinis KCTC $3284^T$ $14{\mu}g/ml$, for S. sanguinis 912 $14{\mu}g/ml$, for Neisseria sp. KEM232 $12{\mu}g/ml$. Conclusions : Even a small amount of extract from lichen Usnea aurantiacoatra KoLRI 004184 showed very high level of antimicrobial activation against all 16 bacterial strains involved in dental caries.

• 한국치위생학회지
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• 제15권2호
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• pp.311-317
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• 2015

Objectives: The purpose of this study was to identify the effects of xylitol and sorbitol mouth rinse on the salivary caries activity levels. Methods: The study subjects were 38 female college students in Gyeongju, Korea. The subjects visited the institution once a week for 4 weeks and the saliva samples were measured for the amount of salivary caries activity levels. The saliva was collected 5 times and incubated in Mitis Salivarius Agar for 48 hours(VS-1203P3L, Vision, Korea) and measured. Results: The Streptococcus mutans CFU decreased by statistically significant amounts as compared to before the experiment within the xylitol group and the sorbitol group(p<0.01). After three weeks of mouth rinse application, Streptococcus mutans CFU of the xylitol group and the sorbitol group showed statistically significant differences(p<0.05). The salivary flow rates within the xylitol group and sorbitol group increased by statistically significant amounts(p<0.01) than before the test. The change in the salivary buffering capacity decreased by a statistically significant amount as compared to before experiment within the xylitol group(p<0.01). Conclusions: The salivary caries activity levels decreased after using xylitol and sorbitol mouth rinse in CFU and the flow rate. The buffering activity levels increased within the xylitol group. Further follow-up studies would be necessary to identify the various effects of xylitol.

• 한국치위생학회지
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• 제17권2호
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• pp.283-294
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• 2017

Objectives: The purpose of the study was to investigate the effect of calcium concentration in saliva on dental caries activity after consuming calcium. Methods: A total of 59 adult women aged 20 to 40 years were surveyed for calcium intake. The daily average calcium intake was analyzed through dietary records of the subjects. The subjects were divided into two groups based on daily average calcium intake. Salivary pH and concentrations of minerals in the saliva were obtained from A group and B group. Calcium ($Ca^{2+}$) and magnesium ($Mg^{2+}$) concentrations in saliva were measured by HPLC-Ion chromatography using 15 mM sulfuric acid. The dental caries activity test was quantified by salivary buffer capacity test and plaque pH test. Results: The mean $Ca^{2+}$ concentrations of A group was $12.75{\mu}g/m$, the mean $Ca^{2+}$ concentrations in the B group was $16.30{\mu}g/mL$ (p<0.05) and respectively, $Mg^{2+}$ concentrations were found to be $0.48{\mu}g/mL$ and $0.51{\mu}g/mL$. Calcium intake and calcium concentration in saliva showed a significant correlation (r=0.380). Conclusions: The mean $Ca^{2+}$ concentrations in saliva was higher in the high calcium intake group. Therefore, calcium intake in saliva was correlated with dental caries.

• 한국치위생학회지
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• 제3권2호
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• pp.197-208
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• 2003

This study was implemented for 84 students of dental hygiene to show the correlation between dental caries experience and improved caries activity test. Dental caries experience for the sample groups was examined and stimulative saliva secreted for 5 minutes was collected into the tube to check saliva secretion rate. Dentocult LB test was executed to observe Lactobacilli colonies after 96 hour cultivation of culture slides moistened with stimulative saliva. Dentocult SM test(screening strip, site strip) was done to measure SM colonies distribution after 48 hour cultivation of culture strips applied with collected saliva and dental plaque respectively, and salivary buffering capacity was checked by means of Dentobuff strip kit. Following conclusions are obtained after examining the relation between Dentocult LB, Dentocult SM, Dentobuff strip test results and DMFT index, salivary secretion rate. 1. Showed no significant difference between Dentocult LB test results and DMFT index, salivary secretion rate. 2. Showed no significant difference between Dentocult SM(screening strip) test results and DMFT index, salivary secretion rate. 3. Showed significant difference between Dentocult SM(site strip) test results and DMFT index(pE0.05), but showed no significant difference between Dentocult SM(site strip) test results and salivary secretion rate. 4. Showed no significant difference between Dentobuff strip test results and DMFT index, but showed a very wide difference between Dentobuff strip test results and salivary secretion rate(pE0.01).

• 대한치과의사협회지
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• 제12권10호
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• pp.767-770
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• 1974

A 12 years old patient having rampant dental caries on permanent dentition has been treated under operative procedures. In addition to this operative treatment the nutritional survey and the caries activity was checked, and performed the oral hygiene instruction to the patient. As the consequences of above procedures the following results were obtained: 1. The familial history and eating habits checkings were helpful for better dental health guide and caries control. 2. The restriction of mono & disaccharide intake and topical application of 8% stannous fluoride at least two times revealed excellent control effect for recurrent dental caries.

• 치위생과학회지
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• 제13권4호
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• pp.403-409
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• 2013

본 연구는 초기우식 진단장비인 QLF-D와 새로운 치아우식활성 검사법인 Cariview를 우식예측 연구에 활용할 수 있는지를 평가하기 위하여, 만 3세 아동 57명을 대상으로 구강검사를 실시한 후 우식경험도를 분석하고 이들 간의 상관성을 평가하여 다음과 같은 결과를 얻었다. 1. 연구대상자의 우식경험도 조사 결과, 우식경험유치지수는 평균 1.84였으며, QLF-D를 이용한 분석 결과는 치면 세균막 부착에 대한 점수가 1.47이었고, 연구대상자 당 평균 1개 정도의 초기우식병소($0.96{\pm}1.21$)가 있는 것으로 나타났다. 또한 Cariview 검사에서 산생성능은 평균 27.52로 나타났고, 연구대상자 중 68%가 저위험군에 속하는 것으로 파악되었다. 2. 우식경험유치지수와 초기우식병소 개수의 상관계수가 0.617, 치면세균막 부착정도(SPS)와는 상관계수가 0.500으로 비교적 강한 상관관계를 확인할 수 있었고(p<0.01), 우식경험유치지수와 Cariview 결과(0.286) 간에는 유의한 양의 상관관계가 나타났다(p<0.05). 그러나 우식유치지수, 우식경험충전유치지수에서는 Cariview 점수와의 상관관계를 확인할 수 없었다. 이상의 결과를 종합해보면, QLF-D를 이용하여 분석한 치면세균막 점수와 초기우식병소는 우식예측에 가장 중요한 영향을 주는 과거 우식경험에 높은 상관성을 보였으므로, 초기우식병소 탐지와 치면세균막 부착 정도를 동시에 파악할 수 있는 QLF-D는 우식예측 연구에 유용한 장비로 활용할 수 있을 것이다.

• 대한소아치과학회지
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• 제33권4호
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• pp.615-623
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• 2006

본 연구는 LED 형광법을 이용하여 각 개인의 우식활성도를 측정할 수 있는지를 규명하기 위해 $6{\sim}7$세의 아동 55명을 대상으로 치아의 순면과 협면에 LED 광을 조사하고 특수 필터를 사용하여 초기 치아우식증이 관찰되는 치아의 수와 우식의 크기, 위치 등을 기록하고, 이와 같은 초기 치아우식증의 수를 측정하는 우식활성검사법과 기존의 우식활성도 측정방법인 dDfFtT rate, Streptococcus mutans colony count와 상관성을 비교, 평가하고, LED 형광법을 이용한 우식활성검사의 특이도, 민감도, 진단력을 평가하였다. 1. LED 형광법을 이용한 우식활성도 측정법은 기존의 Streptococcus mutans colony count 검사법과 비교적 높은 상관관계를 보였다$({\gamma}=0.43,\;P<0.05)$. 2. 육안검사를 기준검사법으로 하였을 때, LED의 특이도, 민감도, 진단력은 각각 100%, 76.1%, 100%이었다. 3. dDfFtT를 기준검사법으로 하였을 때, LED의 특이도, 민감도, 진단력은 각각 88.9%, 47.8%, 95.7%이었다. 4. S. mutans 측정법을 기준검사법으로 하였을 때, LED의 특이도, 민감도, 진단력은 각각 100%, 58.7%, 100%이었다. 이상의 결과를 종합하여 볼 때 LED광중합기를 이용한 광학적 치아우식활성 검사법은 미생물학적 치아우식활성 검사법과 비교하여 유의한 상관관계를 보여 임상에서 활용 가능성이 클 것으로 사료된다.

• 약학회지
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• 제49권2호
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• pp.122-127
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• 2005

Dental caries is one of the most common oral diseases in the world. Glucosyltransferase (Gtase) of Streptococcus mutans (S. mutans) plays an important role in the develo pment of dental caries. For the purpose to develop anti- caries, we examined the effect of metabolites isolated from Streptomyces sp. M-20 on Gtase and the growth of S. mutans. Streptomyces sp. M-20 isolated from Mongolian soil showed 95~96% sequence homology with that of Streptomyces lin- colnensis. The metabolites of Streptomyces sp. M-20 were partially purified by extraction with ethyl acetate, silica gel column chromatography and preparative TLC. Partially purified metabolite, red colored component (MR-20) in ethyl acetate fraction showed potent antibacterial activitiy against S. mutans and inhibitory activity against Gtase purified from S. mutans, while another isolated yellow component (MY-20) showed no activity against S. mutans. The inhibitory activity of MR-20 against Gtase was confirmed by activity staining on SDS-polyacrylamide gel electrophoresis. The concentration of MR-20 for 50% inhibition $(IC_{50})$ against Gtase activity was $60{\mu}g/ml$. These results suggest that MR-20 can be developed for antibacterial agent and anticaries.

• 한국치위생학회지
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• 제4권2호
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• pp.255-263
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• 2004

One of the best way to prevent dental caries and periodontal disease is removing dental plaque. Toothbrushing is also one of the crucial ways to gel rid of dental plaque and improve dental hygiene. The purpose of th is study was to examine how many bacteria were attached to toothbrushes used by dental palque acidogenecity and related behaviors to toothbrush use. Subjects in this study were 30 college students in their twenties, on whom a survey was conducted to find out how they cleansed their toothbrushes, how long they cleaned toothbrushes after brushing, and how to keep them. And then dental palque acidogenecity by caries activity test was implemented and the number of bacteria attached to their toothbrushes was tested. The findings of the study were as follows : 1. Concerning correlations between dental palque acidogenecity and the number of remaining bacteria in the toothbrushes used by students, the number of bacteria was lower in the group of students with no caries activity than in the group with mild caries activity. 2. As for relations between the way of cleansing toothbrushes and the number of bacteria, there was no statistically significant gap between the group of students who wetted their toothbrushes with water and rubbed the heads of them and the group of students who just only wetted their toothbrushes with no further actions. 3. Regarding the relationship of toothbrush cleansing time to the number of bacteria, the number of bacteria was lower in the group of students who cleansed their toothbrushes for 15 seconds or more. 4. As to the influence of the number of toothbrush used by students, there was no significant difference between the group using just one toothbrush and the other group using two or more toothbrushes. 5. As for correlations between the way of keeping toothbrushes and the number of bacteria, no significant gap existed between the group thai kept their toothbrushes separately and the other that didn't.