• Journal of Ecology and Environment
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• 제29권5호
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• pp.433-438
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• 2006

To elucidate the mechanisms by which algal food quality affect Daphnia growths, we measured carbon incorporation rates and respiration rates of Daphnia magna with Cryptomonad Rhodomonas minuta, green algae Scenedesmus acutus and cyanobacteria Synechococcus sp. with varying physiological states as food. Carbon assimilation rates were high with R. minuta and S. acutus and low with Synechococcus sp. showing a similar pattern to the growth rate pattern. There was no clear difference among respiration rates of three algal species. Carbon assimilation rates and respiration rates of D. magna appeared to be independent on Molar C:P ratios in algal foods. Carbon growth efficiencies (incorporated carbon per assimilated carbon amount) were lower when D. magna fed with Synechococcus sp. than fed with R. minuta or S. acutus. Analysis of variance results show that carbon assimilation rates which were sum of incorporation and respiration rates and carbon growth efficiencies were only dependant on species affiliation. Overall, our results showed that algal species with varying ${\omega}3$ polyunsaturated fatty acid content led different carbon incorporation rates and overall carbon assimilation rates of D. magna.

• 대기
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• 제28권2호
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• pp.113-121
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• 2018

In order to monitor greenhouse gases including $CO_2$, various types of surface-, aircraft-, and satellite-based measurement projects have been conducted. These data help understand the variations of greenhouse gases and are used in atmospheric inverse modeling systems to simulate surface fluxes for greenhouse gases. CarbonTracker is a system for estimating surface $CO_2$ flux, using an atmospheric inverse modeling method, based on only surface observation data. Because of the insufficient surface observation data available for accurate estimation of the surface $CO_2$ flux, additional observations would be required. In this study, a system that assimilates aircraft $CO_2$ measurement data in CarbonTracker (CT2013B) is developed, and the estimated results from this data assimilation system are evaluated. The aircraft $CO_2$ measurement data used are obtained from the Comprehensive Observation Network for Trace gases by the Airliner (CONTRAIL) project. The developed system includes the preprocessor of the raw observation data, the observation operator, and the ensemble Kalman filter (EnKF) data assimilation process. After preprocessing the raw data, the modeled value corresponding spatially and temporally to each observation is calculated using the observation operator. These modeled values and observations are then averaged in space and time, and used in the EnKF data assimilation process. The modeled values are much closer to the observations and show smaller biases and root-mean-square errors, after the assimilation of the aircraft $CO_2$ measurement data. This system could also be used to assimilate other aircraft $CO_2$ measurement data in CarbonTracker.

• Journal of Microbiology and Biotechnology
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• 제18권1호
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• pp.124-127
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• 2008

The behavior of Penicillium camembertii and Geotrichum candidum growing in submerged pure cultures on simple (glutamate) or complex (peptones) substrates as nitrogen and carbon sources and lactate as a second carbon source was examined. Similar to the behavior previously recorded on a simple substrate (glutamate), a clear differentiation between the carbon source and the energy source was also shown on peptones and lactate during P. camembertii growth, since throughout growth, lactate was only dissimilated, viz., used for energy supply by oxidation into $CO_2$, whereas peptides and amino acids from peptones were used for carbon (and nitrogen) assimilation. Because of its deaminating activity, G candidum preferred peptides and amino acids to lactate as energy sources, in addition to being assimilated as carbon and nitrogen sources. From this, on peptones and lactate, G candidum grew faster than P. camembertii (0.19 and 0.08 g/l/h, respectively) by assimilating the most readily utilizable peptides and amino acids; however, owing to its lower proteolytic activity, the maximum biomass was lower than that of P. camembertii (3.7 and 5.5 g/l, respectively), for which continuous proteolysis and assimilation of peptides were shown.

• 대기
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• 제22권4호
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• pp.415-427
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• 2012

$CO_2$ is the most important trace gas related to climate change. Therefore, understanding surface carbon sources and sinks is important when seeking to estimate the impact of $CO_2$ on the environment and climate. CarbonTracker, developed by NOAA, is an inverse modeling system that estimates surface carbon fluxes using an ensemble Kalman filter with atmospheric $CO_2$ measurements as a constraint. In this study, to investigate the capability of CarbonTracker as an analysis tool for estimating surface carbon fluxes in Asia, an experiment with a nesting domain centered in Asia is performed. In general, the results show that setting a nesting domain centered in Asia region enables detailed estimations of surface carbon fluxes in Asia. From a rank histogram, the prior ensemble spread verified at observational sites located in Asia is well represented with a relatively flat rank histogram. The posterior flux in the Eurasian Boreal and Eurasian Temperate regions is well analyzed with proper seasonal cycles and amplitudes. On the other hand, in tropical regions of Asia, the posterior flux does not differ greatly from the prior flux due to fewer $CO_2$ observations. The root mean square error of the model $CO_2$ calculated by the posterior flux is less than the model $CO_2$ calculated by the prior flux, implying that CarbonTracker based on the ensemble Kalman filter works appropriately for the Asia region.

• 환경생물
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• 제30권3호
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• pp.173-184
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• 2012

In order to investigate the fluctuation rates [FR] of phytoplankton after passage through a cooling system, the standing crops, chlorophyll a concentrations and carbon assimilation number of phytoplankton were surveyed at intake and outlet at Wolsong nuclear power plant [NPP] from July 2006 to June 2008. As a result, the total mean standing crops of phytoplankton were $1.0{\times}10^6\;cells\;L^{-1}$ and $7.3{\times}10^5\;cells\;L^{-1}$ at intake and outlet, respectively. The FR of phytoplankton by passage through the cooling system [PTCS] was 27.0%. Among them, the FR of microplankton and nanoplankton were 34.1% and 12.4%, respectively. In addition, the FR of diatoms and dinoflagellateswere 33.9% and 29.7%, respectively. These results showed the entrainment effects on microplankton and diatoms by PTCS were higher than those of nanoplankton and dinoflagellates. The FR of total chlorophyll a concentrations were 54.4%, and the FR of microplankton, nanoplankton and picoplankton were 58.9%, 38.5%, and 52.4%, respectively. So the entrainment effects on microplankton by PTCS were higher than those of nanoplankton and picoplankton. The mean FR of carbon assimilation number of phytoplankton was 57.6%, and the seasonal variations of FR of carbon assimilation number ranged from 47.5% to 76.8%. Our results indicated that the phytoplankton species responded differently to power plant operating conditions such as elevated temperature, chlorination, and mechanical impacts.

• 한국작물학회지
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• 제40권5호
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• pp.556-561
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• 1995

등숙기간중 벼의 탄소동화율과 기공전도도 그리고 수용성 탄수화물농도의 내생리듬을 알아보기 위하여 자연상태의 광주기 조건에서 재배된 벼를 일정한 환경조건으로 옮긴 다음 지엽을 대상으로 이들의 반응을 관찰하였다. 자연상태의 광조건에서 성장한 벼의 탄소동화율은 약 24시간을 1주기로 하여 일정한 진폭으로 변화되었으며, 정오에 최고에 달하고 한밤중에 최저에 이르나 72시간이 경화할 때 쯤 변화의 폭은 점차적으로 줄어 들어 120시에는 최고, 최저에 도달하는 시각이 바뀌어 정오에 최저에 달하고 한밤중에 최고에 이르는 현상을 나타내었다. 이와 같은 반응은 기공전도도에서도 찾아볼 수 있어 이들 관계는 매우 밀접함을 보였다. 반면, 개화 후 14일 동안 낮과 밤 모두 일정한 광조건에서 성장한 벼의 탄소동화도율과 기공전도도의 반응은 전자와 같이 주기적인 폭의 변화를 찾아볼 수 없었다. 잎내의 수용성 탄수화물 농도의 변화 또한 탄소동화율과 기공전도도의 변화에서 나타나는 현상과 일치하는 경향을 보였다. 이상에서 탄소동화율과 기공전도도 그리고 수용성 탄수화물의 내생림듬은 작물에 부분적으로 중요한 역할을 하고 있음을 시사하며, 또한 이들은 광주기(photoperiod)에 의존적이기 때문에 광주기가 탄소동화작용 및 수용성 탄수화물 형성에 중요한 조절 요인으로 작용하고 있음을 시사한다.

• Journal of Microbiology and Biotechnology
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• 제11권1호
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• pp.79-84
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• 2001

An aniline-utilizing microorganism identified as a species of Pseudomonas was isolated from soil contaminated highly with aniline and urea-herbicide. This strain was able to utilize aniline as the sole source of carbon and energy, and was shown to harbor a single large plasmid mediating the aniline assimilation. Subsequent plasmid-curing of this bacterium resulted in the abolishment of the aniline utilizing phenotype and the loss of catechol-C2,3O-oxygenase. The reestablishment of the plasmid, denoted pB10, in cured Pseudomonas sp. via filter surface mating, resulted in restoration of the aniline assimilation abilities and enzyme activity.

• Journal of Microbiology and Biotechnology
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• 제33권11호
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• pp.1403-1411
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• 2023

Carbon dioxide (CO₂) is the most abundant component of greenhouse gases (GHGs) and directly creates environmental issues such as global warming and climate change. Carbon capture and storage have been proposed mainly to solve the problem of increasing CO₂ concentration in the atmosphere; however, more emphasis has recently been placed on its use. Among the many methods of using CO₂, one of the key environmentally friendly technologies involves biologically converting CO₂ into other organic substances such as biofuels, chemicals, and biomass via various metabolic pathways. Although an efficient biocatalyst for industrial applications has not yet been developed, biological CO₂ conversion is the needed direction. To this end, this review briefly summarizes seven known natural CO₂ fixation pathways according to carbon number and describes recent studies in which natural CO₂ assimilation systems have been applied to heterogeneous in vivo and in vitro systems. In addition, studies on the production of methanol through the reduction of CO₂ are introduced. The importance of redox cofactors, which are often overlooked in the CO₂ assimilation reaction by enzymes, is presented; methods for their recycling are proposed. Although more research is needed, biological CO₂ conversion will play an important role in reducing GHG emissions and producing useful substances in terms of resource cycling.

• 한국유기농업학회지
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• 제18권2호
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• pp.245-255
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• 2010

본 연구는 여러 유기질 비료와 멀칭자재에 따른 MM.106 사과 대목의 생장과 광합성 동화량에 미치는 영향을 구명하기 위해서 수행되었다. 유기질 비료와 멀칭 자재의 무기성분 함량과 탄소/질소 비율(탄질비)은 수체 생육과 광합성에 영향을 주었다. 미생물이 유기태질소를 무기태로 전환하기 용이한 이상적인 탄질 비를 나타냈던, 상업용 유기질 비료, 계분, 식물성 퇴비, 그리고 풀 멀칭구가 줄기생장과 SPAD 및 광합성 함량을 증가시켰다. 처리 5주 후에 SPAD 및 광합성 함량이 처리구에 의해 영향을 받았으며, 처리 6주 후에 줄기생장 또한 처리구 간에 유의성을 나타냈다. 토양 내 무기태 질소 함량이 $10{\sim}15mg{\cdot}kg^{-1}$이었을 때 가장 효율적인 수체 생장과 발달을 나타냈으며, 질소함량은 생장과 발달에 유의적인 상관관계를 나타냈다.

• KSBB Journal
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• 제13권4호
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• pp.391-398
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• 1998

The effect of culture medium copper availability on the specific growth rate(${\mu}$) and carbon conversion efficiency (CCE) was sutided for an obligatory methanotroph Methylosinus trichosporium OB3b under various combinations of carbon and nitrogen sources. Methane or methanol was used as a carbon source, and nitrate or ammonium was used as a nitrogen source. Medium copper availability determined the intracellular location or kind of methane monooxygenase (MMO), cell-membrane (particulate or pMMO) when copper was present and cytoplasm (soluble or sMMO) when copper was deficient. When methane was used as a carbon source, copper-containing medium exhibited higher ${\mu}$ and CCE than copper-free medium regardless of the kind of nitrogen source. When methanol was used as a carbon source, however, the effect of copper disappeared. Ammonium gave the higher ${\mu}$ and CCE than nitrate for both methane and methanol. Those observation suggest that there exist an important difference in energy utilization efficiency for methane assimilation between sMMO and pMMO.