• Title/Summary/Keyword: Carbohydrates

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Effects of Antioxidants on the Photosynsthesis and Carbohydrates/Saponin Contents in Panax ginseng Leaves (인삼잎의 광합성과 탄수화물.사포닌 함량에 미치는 항산화제의 효과)

  • 양덕조;김용해
    • Journal of Ginseng Research
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    • v.18 no.3
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    • pp.175-181
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    • 1994
  • We studied the folilar wiping effects of antioxidants (ascorbate, glutathione and sodium azide), which effectively inhibited the chlorophyll bleaching or completely recorved the early stage of photosynthesis of Panax ginseng C.A. Meyer, on photosynthesis, stomatal resistance, free sugar, starch, and total saponin contents of ginseng under the excess light intensity (45 kLux) during 6 days. Ascorbate and glutathione, endogenous antioxidant, recovered photosynehtsis and stomatal resistance, and reduced the photoinhibition by the excess light intensity (45 kLux) on free sugar, starch and total saponin contents. But sodium azide, exogenous $^{1}O_2$ quencher, showed negative effect. Therefore, we assumed that carbohydrates and saponin metabolisms of ginseng by antioxidants (ascorbate, glutathione) were normal. For the reduction of inhibition by excess light in ginseng a program for the higher activation of antioxidants and antioxidative enzymes in ginseng leaf will be desirable. Key words Antioxidants, ascorbate, glutathione, Photoinhibition, ginseng.

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Regulation of Growth and Metabolic Activities of Chlorella fusca by Release Products of Some Aquatic Fungi (수생균의 분비물질에 의한 Chlorella fusca의 성장 및 대사조절)

  • Hassan, S.K.M.;Fadl-Allah, E.M.;Kobbia, I.A.;Shoulkamy, M.A.
    • The Korean Journal of Mycology
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    • v.18 no.4
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    • pp.181-190
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    • 1990
  • The growth and biochemical activities of Chlorella fusca were studied in the presence of different concentrations of either filtrates or mycelial mats of Saprolegnia ferax and Pythium graminicola. Low concentrations of both fungal filtrates exerted increase in total count, dry weight and in the biosynthesis of photosynthetic pigments, carbohydrates and nitrogen content. High concentrations showed inhibitory effect on both growth and biochemical activities of Chlorella fusca. Supplementation with different concentrations of dry mycelial mats of either fungi the culture of Chlorella showed elevation in biomass, dry weight, and biosynthesis of carbohydrates and nitrogen content especially at low concentrations. The contents of photosynthetic pigment were inhibited only at low concentrations. Neither the culture filtrate of Pythium nor Saprolegnia had cellulolytic activity, although polygalacturonase enzymes were detected, whereas chloroform-extract of both fungal filtrates showed blue spots under long wave light (366 nm).

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Amylolytic Enzymes Produced from Hyperthermophilic Archaebactorium Thermococcus profundus (고도 호열성 Archaebacterium Thermococcus profundus가 생산하는 Amylolytic Enzymes)

  • Jeong, Yeong-Cheol;Kim, Gyeong-Suk;No, Seung-Hwan
    • The Korean Journal of Food And Nutrition
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    • v.7 no.4
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    • pp.259-266
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    • 1994
  • The hyperthermophilic archaebacterium Thermococcus profundus Isolated from a deep-sea hydrothermal vent system, produced several amylolytic enzymes such as extracellular amylase and pullulanase, intracellular a-1,4-91ucosidase in respone to the presence of complex carbohydrates In the growth medium. This strain showed high activities on 0.5% maltose than on complex carbohydrates One of the amylases was partially purified by ammonium sulfate precipitation, DEAE-Toyopearl chromatography. The amylase exhibited maximal activity at pH 5.5 and 80$^{\circ}C$, and was stable in the range of pH 5.5 to 9.5 and up to 80$^{\circ}C$ for 30 min. The enzyme activity was no dependence on Ca2+ and not inhibited by detergents. The amylase hydrolyzed soluble starch, amylose, amylopectin and glycogen to produce maltose and maltotriose with trace amounts of glucose, but not pullulan and ${\alpha}$-, ${\beta}$-, ${\gamma}$-cyclodextrin. Malto-oligosaccharides ranging from maltotetraose to maltoheptaose were hydrolyzed in an endo fashion.

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Chemometric A spects of Sugar Profiles in Fruit Juices Using HPLC and GC

  • 윤정현;김건;이동선
    • Bulletin of the Korean Chemical Society
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    • v.18 no.7
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    • pp.695-702
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    • 1997
  • The objective of this work is to determine the sugar profiles in commercial fruit juices, and to obtain chemometric characteristics. Sugar compositions of fruit juices were determined by HPLC-RID and GC-FID via methoxymation and trimethylsilylation with BSTFA. The appearance of multiple peaks in GC analysis for carbohydrates was disadvantageous as described in earlier literatures. Fructose, glucose, and sucrose were major carbohydrates in most fruit juices. Glucose/fructose ratios obtained by GC were lower than those by HPLC. Orange juices are similar to pineapple juices in the sugar profiles. However, grape juices are characterized by its lower or no detectable sucrose content. In addition, it was also found that unsweeten juices contained considerable level of sucrose. Chemometric technique such as principal components analysis was applied to provide an overview of the distinguishability of fruit juices based on HPLC or GC data. Principal components plot showed that different fruit juices grouped into distinct cluster. Principal components analysis was very useful in fruit juices industry for many aspects such as pattern recognition, detection of adulterants, and quality evaluation.

The Relationship between the Sugar Preference of Bacterial Pathogens and Virulence on Plants

  • Ismaila Yakubu;Hyun Gi Kong
    • The Plant Pathology Journal
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    • v.39 no.6
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    • pp.529-537
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    • 2023
  • Plant pathogenic bacteria colonize plant surfaces and inner tissues to acquire essential nutrients. Nonstructural sugars hold paramount significance among these nutrients, as they serve as pivotal carbon sources for bacterial sustenance. They obtain sugar from their host by diverting nonstructural carbohydrates en route to the sink or enzymatic breakdown of structural carbohydrates within plant tissues. Despite the prevalence of research in this domain, the area of sugar selectivity and preferences exhibited by plant pathogenic bacteria remains inadequately explored. Within this expository framework, our present review endeavors to elucidate the intricate variations characterizing the distribution of simple sugars within diverse plant tissues, thus influencing the virulence dynamics of plant pathogenic bacteria. Subsequently, we illustrate the apparent significance of comprehending the bacterial preference for specific sugars and sugar alcohols, postulating this insight as a promising avenue to deepen our comprehension of bacterial pathogenicity. This enriched understanding, in turn, stands to catalyze the development of more efficacious strategies for the mitigation of plant diseases instigated by bacterial pathogens.