• BMB Reports
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• 제50권11호
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• pp.554-559
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• 2017

MicroRNAs (miRNAs) are ~22nt-long single-stranded RNA molecules that form a RNA-induced silencing complex with Argonaute (AGO) protein to post-transcriptionally downregulate their target messenger RNAs (mRNAs). To understand the regulatory mechanisms of miRNA, discovering the underlying functional rules for how miRNAs recognize and repress their target mRNAs is of utmost importance. To determine functional miRNA targeting rules, previous studies extensively utilized various methods including high-throughput biochemical assays and bioinformatics analyses. However, targeting rules reported in one study often fail to be reproduced in other studies and therefore the general rules for functional miRNA targeting remain elusive. In this review, we evaluate previously-reported miRNA targeting rules and discuss the biological impact of the functional miRNAs on gene-regulatory networks as well as the future direction of miRNA targeting research.

• 한국환경복원기술학회지
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• 제20권2호
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• pp.13-35
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• 2017

This study was carried out to analyze vegetation structure and change of the disturbed area in forest ecosystem(FE), riparian ecosystem(RE) and coastal ecosystem(CE) related to Regional Ecological Network at Chungnam province through constancy and dominance analysis, species diversity index, similarity index and canonical correlation analysis. Data were collected from April to October in 2015. As a result of constancy and dominance analysis of forest disturbed area, non-effective species(NES) was 30 species(17.0%), exported species(ES) was 98 species(55.7%) and imported species(IS) was 48 species(27.3%) among the total 176 species, respectively. In riparian disturbed area with total 139 species, there were 16 NES(11.5%), 98 ES(70.5%) and 25 IS(18.0%) respectively. In coastal disturbed area with 140 species, there were 20 NES(14.3%), 88 ES(62.9%) and 32 IS(22.9%) respectively. In all types of disturbed areas, the ratio of ES was higher than IS. As a result of species diversity, species richness and shannon's diversity index of disturbed area decreased in all kinds of crown strata such as tree, subtree, shrub and herb layer compared to the control area. As a result of similarity index, that of each type between control site and disturbed site was 0.374 in FE, 0.329 in CE and 0.259 in RE in the order. As a result of the CCA analysis, the number of present species, vine plants ratio and exported species ratio in disturbed area of FE and RE were decreased, and the naturalized plant ratio, imported species ratio and herb ratio were increased. But environmental factors of CE were not shown any clear tendency. In conclusion, many species occupied in control site disappeared into the disturbed area, and the naturalized plants and herb species were abundantly imported in the area. Therefore, it was considered that this study could be applied to the development of long-term and short-term ecological restoration techniques in view of vegetation changes.

• 한국산림과학회지
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• 제111권2호
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• pp.201-223
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• 2022

오늘날 급격한 기후 변화와 지속적인 인간의 개발 압력으로 멸종의 우려에 대한 심각성이 커지고 있다. 본 연구에서는 우리나라의 커다란 생태축인 백두대간 일대에서 출현하는 식물 가운데 중점적으로 보전해야할 식물 300종을 우선적으로 선정하였다. 그중 석회암 지대에 출현하는 댕강나무를 최우선 보전종으로 인식하고 이들의 식생 군집과 환경인자 특성을 구명하고자 하였다. 단양군, 영월군, 제천시 등 댕강나무 서식지 36개소를 조사지로 설정하고, 조사지 내 출현식물, 식생, 토양과 물리적 환경을 분석하였다. 조사지에서 특기할 만한 식물로 꽃꿩의다리, 덕우기름나물, 나도국수나무 등이 출현하였다. 산림식생 군락유형은 4개의 식생유형과 7개의 종군유형으로 구분되었다. 식생군집과 환경인자의 CCA 분석결과, 종합설명력 75.2%였으며 댕강나무 서식지의 환경적 특징이 세 그룹으로 구분되었다. 이 중에서 관계성이 있었던 환경인자는 해발고도, 경사, 유기물, 암석 비율, pH, 칼륨, 그리고 나트륨이었다. 조사지 내에서 꽃꿩의다리를 비롯하여 다수의 희귀식물과 특산식물이 확인되어 이들 집단을 서식지 수준에서 보전할 필요가 있다고 판단하였다. 출현 식물을 토대로 분석된 식생 유형 분류와 CCA 분석에서 댕강나무 서식지의 식생 집단의 고유성과 특이성이 재차 인정되었다. 본 연구의 결과가 댕강나무 자생지의 실증적 보전을 위한 과학적 근거자료로 활용되길 기대한다.