• Asian-Australasian Journal of Animal Sciences
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• 제17권9호
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• pp.1286-1290
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• 2004

This study was carried out to screen yeasts effective in reducing odor from pig feces. Three isolates from soil and compost sources were selected to treat pig feces. On the basis of morphological and biochemical characteristics, one isolate from compost was Candida rugosa, and two isolates from soil were Candida rugosa and Candida maris. These isolates showed deodorizing activity by reducing the concentration of ${NH}_3$ and R-${NH}_2$. Volatile fatty acids (VFA) are the specific malodorous compounds of pig feces, and the Candida maris from soil showed a 100% reduction of butyric, iso-butyric, and iso-valeric acid in 10% pig slurry medium. However, the Candida rugosa from compost showed a 100% reduction of butyric and iso-butyric acid while that from soil showed a 100% reduction of propionic, butyric acid and iso-valeric acid in the medium. Also, these yeasts were effective in reducing $NH_{4}-N$, soluble-N, and biological oxygen demand (BOD).

• 유기물자원화
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• 제12권3호
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• pp.119-125
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• 2004

사탕무즙을 이용한 알콜생산에서 배출되는 증류폐액(sugar beet stillage)을 기질로 고온성 효모인 Candida rugosa를 이용해 단세포단백을 생산 할 때 기질의 최적화와 COD 감소를 연구하였다. 인의 첨가는 실험에 사용된 3가지 모든 시료기질에 꼭 필요한 것으로 나타났고 질소첨가는 기질의 잔당농도가 높을 경우에 필요하였다. 모든 시료에 있어서 인의 첨가는 생균생산율(Biomass production)을 23-61% 까지 크게 높였다. 이에 비해 질소의 첨가는 잔당함량이 높을 경우 생균생산율을 약간 상승시켰으나 질소와 함께 첨가할 경우 90%까지 크게 높였다. COD는 인의 첨가로 인해 26-46%까지 더 감소되었으며 질소와 함께 첨가할 경우 85%까지 크게 감소되었다.

• 한국미생물·생명공학회지
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• 제28권4호
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• pp.223-227
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• 2000

A novel two-step acetone treatment method was developed to enhance the enantioselectivity of Candida rugosa lipase (CRL) toward the hydrolysis of racemic naproxen methyl ester. The acetone-teated CRL was considerably more enantioselective than the crude CRL, yielding an enantiomeric excess of 98~100%. The crude and acetone-treated CRLs were subjected to anion exchange chromatography, and their chromatography profiles were compared. In consequence, both chromatography profiles were found to be almost identical, resulting in two separate lipase peaks (lipase A and B). The lipase B, which is known to be less enantioselective, was treated with acetone using a two-step treatment method. The enantioselectivity of acetone-treated lipase B was dramatically increased, yielding an enantiomeric excess of 99%.

• Journal of Microbiology and Biotechnology
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• 제1권3호
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• pp.207-211
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• 1991

Yeast fermentation at $40^{\circ}C$ was conducted for microbial protein production and COD reduction in three different sugar beet stiIlages by a thermo- and acid-tolerant yeast Candida rugosa isolated from East Africa. The assimilation proceedings of some main components such as protein, carbohydrate, total titrable acids and glycerol in stillages were observed with growth kinetics of the yeast. Most of glycerol and organic acids were rapidly assimilated at the beginning of the fermentation. Protein assimilation was slowly accelerated with the proceeding of fermentation time and its assimilation rate reached only 14.2%-28.4%. Though Candida rugosa was a flocculent yeast, the flocculation characteristics of the yeasts grown in three stillages were different from each other.

• 한국미생물·생명공학회지
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• 제21권3호
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• pp.281-287
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• 1993

Rye stillage was adopted as a substrate for the production of yeast biomass by a thermotolerant yeast Candida rugosa isolated from East Africa. In the batch fermentation, the yield of biomass and crude protein reached 4.9-8.4g/l and 2.2-3.5g/l, respectively, the rate of COD reduction was about 20%. Over 90% amount of main components such as glycerol and lactic acid were assimilated, but protein assimilation reached only to 38-45% of the initial content. Crude protein content of the dry yeast biomass produced was 42-47% and sulfur-containing amino acid was revealed as limiting essential amino acid.

• 한국미생물·생명공학회지
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• 제28권6호
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• pp.355-360
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• 2000

Candida rugosa IFO0750의 UV-변이주를 제조하여 butyric acid를 D-$\beta$-hydroxybutyrin acid(이하 D-$\beta$-HBA)로 전환하는 데 이용하였다. 후보 변이주 중 활성이 가장 높은 Candida rugosa CM42를 이용하여 발효를 수행한 후 NMR 분석, polarimeter 분석 등을 통하여 생성된 물질이 D-$\beta$-HBA 임을 확인하였다. Chemostar 배양을 이용하여 D-$\beta$-HBA 발효 생산의 주요 영향인자를 분석하였으며, 균체의 활성을 나타내는 비생산성의 최대치는 균체의 비증식 속도를 0.06, 발효조 내의 glucose와 butyric acid의 농도를 각각 10g/L와 8.7 g/L로 각각 유지 할 때 얻어졌다. 회분식 배양 중에 glucose와 butyric acid를 공급하여 발효조 내의 glucose 및 butyric acid 농도를 최적조건으로 유지하는 fed-batch 발효를 수행하였다. 배양 180 시간 후에 D-$\beta$-HBA 농도가 약 12.4 g/L에 도달하였으며 회분식 발효에 비하여 4.7배 증가하였다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권6호
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• pp.522-525
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• 2006

Biodiesel conversion from soybean oil reached a maximum of 70% at 18 h using immobilized 1,3-specific Rhizopus oryzae lipase alone. Biodiesel conversion failed to reach 20% after 30 h when immobilized nonspecific Candida rugosa lipase alone was used. To increase the biodiesel production yield, a mixture of immobilized 1,3-specific R. oryzae lipase and nonspecific C. rugosa lipase was used. Using this mixture a conversion of greater than 99% at 21 h was attained. When the stability of the immobilized lipases mixture was tested, biodiesel conversion was maintained at over 80% of its original conversion after 10 cycles.

• 한국축산시설환경학회지
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• 제8권1호
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• pp.17-24
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• 2002

본 연구는 돈분 완숙퇴비에서 돼지분뇨 악취제어 효과가 뛰어난 효모를 분리하고, 그들의 특성을 조사하여 악취미생물에 관한 기초자료를 얻고자 수행하였다. 10% 돈분배지에서 성장이 우수한 균주를 선발하고 10% 돈분액체배지에 접종하여 28$^{\circ}C$ 72시간 배양한 후 가스경지간을 이용하여 암모니아와 아민류를 측정하여 이들에 대한 기질 선호성을 가진 호모를 악취유용 미생물로 CY-10을 선발하였다. 이의 형태적, 생화학적 특성에 따라 희비에서 분리한 CY-10은 Candida rugosa로 동정되어 본 시험에 공시로 Candida rugosa CY-10으로 하였다. 이의 악취저감능을 조사하기 위해서 돈분 액체배지에 성장시키면서 이들 배양액의 휘발성 지방산을 분석한 결과 대조구에 비해 CY-10의 경우 iso- butric acid와 busic acid를 100% 감소시켜 뷰틸산류에 기질특이성이 있는 것으로 나타났으며 또한 이의 처리구는 대조구에 비해 $NH_4$-N, Soluble-N, BOD즐 각각 20%, 12.6%, 9.82%를 감소시키는 효과가 있었다

• KSBB Journal
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• 제14권2호
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• pp.225-229
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• 1999

본 연구에서는 광학적으로 순수한 S-(+)-ketoprofen을 얻기 위한 효소적 분할공정을 개발하는 것을 그 내용으로 하고 있다. 결과에 의하면 Candida rugosa 유래의 lipase는 음이온 교환수지에 의해 두 가지 형태의 효소로 분리되었으며 특히 첫 번째 peak의 lipaserk (S)-enantiomer에 대해 선택성이 매우 높은 것으로 나타났다. 온도 pH, 첨가제의 영향에 대해서 조사한 결과 높은 활성을 유지하면서 광학적으로 순수한 (S)-ketoprofen을 얻는 조건을 찾지는 못하였으나, $37^{\circ}C$의 온도에서 선택성이 높았으며 ethylen glyco과 같은 polyalcohol 종류가 첨가될 경우 선택성이 증가한다는 결과를 얻을 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제5권2호
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• pp.114-116
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• 1995

Concentrated Brazillian sugar cane stillage was used as a substrate for the yeast biomass production using Candida rugosa isolated from East Africa. At the optimum stillage concentration of 10% dry matter, biomass production was 20.4 g/l and COD reduction rate was 41%. The specific growth rate of the yeast was 0.17 $h^{-1}$ and the corresponding productivity 0.91 g $l{-1} h^{-1}$ in the batch fermentation was observed at $40{\circ}^C$.