• Journal of Integrative Natural Science
• /
• v.1 no.1
• /
• pp.19-23
• /
• 2008

Morus alba has been well known for its tonic effect or preventing of hypertension, aging, or diabetes mellitus in Asian countries. To evaluate the its effect on hair growth promotion, we performed the hair regrowth experiment with leaf, branch, root and fruit extract of Morus alba on animal model of C57BL/6 mouse. The hair regrowth effect was remarkable in the mouse treated branch and root extract (with what %), however, little effect on hair regrowth appeared on the mouse treated with leaf extract. Morus alba extract displayed antibacterial effects on Bacillus subtilis, Staphylococcus aureus, Pseudomonas and Candida albicans. These results indicated that branch and root extract of Morus alba could be applicable for hair regrowth or prevention of hair loss in human.

• Bulletin of the Korean Chemical Society
• /
• v.32 no.1
• /
• pp.229-238
• /
• 2011

A series of novel glucose derived benzyl and alkyl 1,2,3-triazoles and their hydrochlorides have been synthesized via Cu(I)-catalyzed 1,3-dipolar cycloaddition. All the new compounds were characterized by MS, IR and NMR spectra. The DEPT, APT, $^1H$-$^1H$ and $^1H-^{13}C$ 2D NMR spectra for some compounds were also recorded. These compounds were evaluated for their in vitro antibacterial activities against Staphylococcus aureus ATCC 29213, Bacillus subtilis, Bacillus proteus, Pseudomonas aeruginosa, Escherichia coli ATCC 25922, and antifungal activities against Candida albicans and Aspergillus fumigatus. The bioactive data revealed that (3R,4S,5S,6S)-2-(hydroxymethyl)-6-methoxy-4,5-bis((1-octyl-1H-1,2,3-triazol-4-yl)methoxy)-tetrahydro-2H-pyran-3-ol 8a exhibited excellent antifungal activity against A. fumigatus with an MIC value of 0.055 mM compared to Fluconazole. It also showed broad inhibitory efficacy against tested bacterial strains with MIC values ranging from 0.049 mM to 0.39 mM.

• Journal of Oral Medicine and Pain
• /
• v.19 no.1
• /
• pp.9-15
• /
• 1994

This experiment was performed to confirm the hypothesis that LLLT had biostimulation effect for all kinds of cells and there would be differences in the growth of cells among different types of pulsed laser. 360 samples were used in this study. The samples were randomly divided in 6 groups according to the pulse type : quasi continuous type (CW), pulse 1(P1), pulse 7(P7), pulse 9(P9), pulse 15(P15) and shame-irradiated control(Co) groups. Energy fluences of all experimental groups, P1, P7, P9, P15 and CW were 2.12, 2.12, 6.37, 57.32 and 31.85 mj/cm2 respectively. All samples were irradiated for every 1 minute at 0, 12, 24, 36, 48 and 60 hours. Ten samples of each group were sacrificed at 0 and every 12 hours and then the optical density of all samples was measured with the spectrophotometer. As a result, some types of pulses showed significant differences among groups. The increase of cells were markedly stimulated with laser irradiation in P7 and P9 groups, while inhibited in CW, P1, and P15 groups compared with control group. It is therefore, suggested that specific laser pulse should be recommanded to have the biostimulation effects on the specific tissue or cells, although the biostimulation effect is does dependant.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.9 no.3
• /
• pp.223-228
• /
• 2004

Agricultural waste products, beech wood and walnut shells, were hydrolyzed at 40$^{\circ}C$ using mixed crude enzymes produced by Penicillium sp. AHT-1 and Rhizomucor pusillus HHT-1. D-xylose, 4.1 g and 15.1 g was produced from the hydrolysis of 100 g of beech wood and walnut shells, respectively. For xylitol production, Candida tropicalis IFO0618 and the waste product hydrolyzed solutions were used. The effects on xylitol production, of adding glucose as a NADPH source, D-xylose and yeast extract, were examined. Finally, a 50% yield of xylitol was obtained by using the beech wood hydrolyzed solution with the addition of 1% yeast extract and 1% glucose at an initial concentration.

• Journal of the Korean Chemical Society
• /
• v.56 no.3
• /
• pp.334-340
• /
• 2012

A new series of ethyl 2-(4-substitutedbenzylidene)-5-(3'-(ethoxycarbonyl)biphenyl-4-yl)-7-methyl-3-oxo-3,5-dihydro-2H-thiazolo[3,2-a]pyrimidine-6-carboxylate derivatives ($\mathbf{8a-j}$) were synthesized. The newly synthesized compounds were characterized by $\mathbf{IR}$, $^1\mathbf{H}$ $\mathbf{NMR}$, $^{13}\mathbf{C}$ $\mathbf{NMR}$, $\mathbf{LCMS}$ $\mathbf{mass}$ and $\mathbf{C}$, $\mathbf{H}$, $\mathbf{N}$ analyses. All newly synthesized compounds were screened for their In vitro antioxidant activity (Scavenging of hydrogen peroxide, Scavenging of nitric oxide radical, and Lipid peroxidation inhibitory activity), antibacterial (Escheria coli, Pseudonmonas aeruginosa (gram-negative bacteria), Bacillus subtillis, Staphylococcus aureus (gram-positive bacteria)) and antifungal (Candida albicans Aspergillus niger) studies.

• The Korean Journal of Mycology
• /
• v.1 no.1
• /
• pp.29-33
• /
• 1973

The discovery of ideal therapeutics of fungal infections are remain a major problems of several mycoses. The antifungal activities of a new antifungal antibiotic named Siccanin and Azalomycin-F studies in vitro against some various species of fungi especially dermatophytes. The antifungal activity tests were performed according to the tube-dilution method and all subcultures were incubated at room temperature for 14 days. Results were obtained as follows: 1. All of the Candida spp. were grow on the various concentration of Siccanin tested but Azalomycin-F were growth inhibited at 7mcg-10mcg per ml. 2. Trichophyton spp. and Microsporum spp. were inhibited for growth at 2mcg-6mcg per ml. and 3mcg-5mcg per ml. concentration of Siccanin and 1mcg-4mcg per ml. of Azalomycin-F. 3. Deep mycoses and some saprophytes were grow on the all tested concentration of Siccania and Azalomycin-F.

• Mycobiology
• /
• v.37 no.2
• /
• pp.141-146
• /
• 2009

Novel copper(II) complexes have been synthesized from the macrocyclic Schiff bases derived from Knoevenagel condensed ${\beta}$-ketoanilides (obtained by the condensation of acetoacetanilide and substituted benzaldehydes), 4-aminoantipyrine and ophenylene diamine. The structural features have been determined from their analytical and spectral data. All the Cu(II) complexes exhibit square planar geometry. Their high molar conductance values support their 1 : 2 electrolytic nature. The magnetic moment data provide evidence for the monomeric nature of the complexes. The X-band ESR spectra of the |$CuL^1$|$(OAc)_2$ in DMSO solution at 300 and 77 K were recorded and their salient features are reported. The in vitro biological screening effects of the investigated compounds were tested against the bacterial species Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris and Pseudomonas aeruginosa and fungal species Aspergillus niger, Rhizopus stolonifer, Aspergillus flavus, Rhizoctonia bataicola and Candida albicans by well diffusion method. A comparative study of inhibition values of the Schiff bases and their complexes indicate that complexes exhibit higher antimicrobial activity than the Schiff bases. Copper ions proved to be essential for the growth-inhibitor effect. The extent of inhibition appeared to be strongly dependent on the initial cell density and on the growth medium.

• Archives of Pharmacal Research
• /
• v.19 no.3
• /
• pp.197-200
• /
• 1996

6-[(N-2,3-Dichlorophenyl)aminol-7-chloro-5,8-quinolinedione (RCK1 1) was tested for in vivo antifungal activities in the treatment of systemic infection with Canclicla albicans in normal mice compared with ketoconazole. The therapeutic potential of RCK11 had been assessed by evaluating their activities (survival rates) against systemic infections in normal mice. $ED_{50}$ of intraperitoneally administered RCK11 was $0.10\pm0.01 mg/kg$ but that of ketoconazole had $8.00\pm0.73 mg/kg$ respectively. When RCK11 was administered intravenously at the $ED_{50}$(0.10 mg/kg), the colony counts of Canoliola albicans in the liver after 7 days and 14 days were reduced as likely as ketoconazole at the $ED_{50}$ (8.00 mg/kg), and the better survival rates than ketoconazole were achieved after 14 days. The results suggest that RCK11 may be a potent antifungal agent.

• YAKHAK HOEJI
• /
• v.54 no.1
• /
• pp.22-26
• /
• 2010

We studied the antimicrobial activities of five compounds isolated from the stems of Vitis coignetiae Pulliat and the seeds of Perilla frutescens (L.) Britton. Based on spectroscopic evidence, compounds 1 to 5 were characterized as resveratrol, $\varepsilon$-viniferin, ampelopsin E, apigenin, and luteolin, respectively. The antimicrobial activities against Gram-positive (Staphylococcus aureus) and -negative (Pseudomonas aeruginosa) bacteria and a fungus (Candida albicans) were investigated using the disc diffusion and broth dilution methods. C. albicans was not inhibited by the five compounds. Compounds 2 and 5 had significant anti-microbial activity against S. aureus, and the 50% inhibitory concentration ($IC_{50}$) of compound 2 against S. aureus was 7.2 ${\mu}M$. Compounds 4 and 5 significantly inhibited P. aeruginosa and the minimum inhibitory concentration (MIC) of compounds 2 and 5 was 0.07 and 2.0 ${\mu}M$, respectively. Compounds 2, 4, and 5 had strong anti-microbial activity against S. aureus and P. aeruginosa.

• YAKHAK HOEJI
• /
• v.57 no.2
• /
• pp.119-124
• /
• 2013

The aim of this present study was to investigate the antifungal effect of tanshinones isolated from Salvia miltiorrhiza against Candida ablicans, a polymorphic fungus. For the work, tanshinone IIA (TSN), cryptotanshinone (CTS), and dihydrotanshinone I (DTS) were chosen. Initially, their antifungal effect was analyzed by in-vitro susceptibility test. Data from the susceptibility test showed that while all of these three compounds had antifungal activity, DTS was the most potent. At $100{\mu}g$ DTS/ml, there was about 80% CFU (colony forming unit) reduction as compared to DTS-untreated C. albicans yeast cells (P<0.05). Thus, DTS was selected to determine its antifungal activity in a murine model of disseminated candidiasis due to C. albicans. Results showed that DTS enhanced resistance of mice against disseminated candidiasis. During the entire period of 30-day observation, 60% of DTS-given mice groups survived whereas control animals all died within 14 days (P<0.05). Moreover, DTS inhibited the hyphal production, one of the virulence factors of this fungus, from the blastoconidial form of the fungus. Therefore, the tanshinone appears to have antifungal activity specific for C. albicans infection, which could possibly be mediated by the blockage of hyphal production.