• Archives of Plastic Surgery
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• v.32 no.2
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• pp.143-148
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• 2005

Chemotactic migration of bone forming cell, osteoblast, is an important event during bone formation, bone remodeling, and fracture healing. Migration of cells is mediated by adhesion receptors, such as integrins, that link the cell to extracellular matrix ligands, type I collagen, fibronectin, laminin and depend on interaction between integrin and extracellular ligand. Our study was designed to investigate the effect of extracellular matrix like fibronectin, laminin, type I collagen on migration of osteoblast. Migration distance and speed of MC3T3-E1 cell on extracellular matrix-coated glass were measured for 24 hours using 0.01% type I collagen, 0.01% fibronectin, 100 microliter/ml laminin. The migration distance and speed of MC3T3-E1 cell was compared using a video-microscopy system. To determine migration speed, cells were viewed with a 4 phase- contrast lens and video recorded. Images were captured using a color CCD camera and saved in 8-bit full-color mode. The migration distance on 0.01% type I collagen or 0.01% fibronectin was longer than that on $100{\mu}l/ml$ laminin-coated glass. The migration speed on fibronectin-coated glass was 68 micrometer/hour which was fastest. The migration speed on type I collagen-coated glass was similar with that on fibronectin-coated glass. The latter two migration speeds were faster than that on no-coated glass. On the other hand, the average migration speed on laminin-coated glass was 37micrometer/hour and not different from that of control group. In conclusion, the extracelluar matrix ligands such as type I collagen and fibronectin seem to play an important role in cell migration. The type I collagen or fibronectin coated scaffold is more effective for migration of osteoblast in tissue engineering process.

• Journal of the Society of Disaster Information
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• v.14 no.4
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• pp.501-509
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• 2018

Purpose: UAV-based photo measurements are being researched using UAVs in the space information field as they are not only cost-effective compared to conventional aerial imaging but also easy to obtain high-resolution data on desired time and location. In this study, the UAV-based high-resolution images were used to perform the land cover classification. Method: RGB cameras were used to obtain high-resolution images, and in addition, multi-distribution cameras were used to photograph the same regions in order to accurately classify the feeding areas. Finally, Land cover classification was carried out for a total of seven classes using created ortho image by RGB and multispectral camera, DSM(Digital Surface Model), NDVI(Normalized Difference Vegetation Index), GLCM(Gray-Level Co-occurrence Matrix) using RF (Random Forest), a representative supervisory classification system. Results: To assess the accuracy of the classification, an accuracy assessment based on the error matrix was conducted, and the accuracy assessment results were verified that the proposed method could effectively classify classes in the region by comparing with the supervisory results using RGB images only. Conclusion: In case of adding orthoimage, multispectral image, NDVI and GLCM proposed in this study, accuracy was higher than that of conventional orthoimage. Future research will attempt to improve classification accuracy through the development of additional input data.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.29 no.3C
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• pp.419-425
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• 2004

This paper presents a new method which is calculated to use only three vanishing points in order to compute the dimensions of object and its pose from a single image of perspective projection taken by a camera. Our approach is to only compute three vanishing points without informations such as the focal length and rotation matrix from images in the case of perspective projection. We assume that the object can be modeled as a linear function of a dimension vector v. The input of reconstruction is a set of correspondences between features in the model and features in the image. To minimize each the dimensions of the parameterized models, this reconstruction of optimization can be solved by standard nonlinear optimization techniques with a multi-start method which generates multiple starting points for the optimizer by sampling the parameter space uniformly.

• Progress in Medical Physics
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• v.12 no.2
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• pp.103-112
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• 2001

Purpose: The purpose of this study was to investigate the current status of performing nuclear medicine quality control in korea and to test selected protocols of quality control of nuclear medicine counting system and gamma camera. Materials and Methods: Fifty three hospitals were included to investigate the current status of nuclear medicine quality control in korea. The precision of dose calibrator and thyroid uptake system was measured with Tc-99m 35.52 MBq for 2 minuets and Tc-99m 5.14 MBq for 10 sec every one minute, respectively. The sensitivity of CeraSPECT$^{TM}$ with low energy high resolution parallel hole collimator was measured using two cylindrical phantoms with 15 cm in diameter and 12 cm and 30 cm in heights containing Tc-99m. The correction factor for sensitivity of CeraSPECT$^{TM}$ was calculated using phantom data. The system planar sensitivity, uniformity, count rate and spatial resolution were measured for Varicam gamma camera with low energy high resolution parallel hole collimator using 140 keV centered 20% energy window, 256$\times$256 or 512$\times$512 matrix sizes. Results: The quality control of dose calibrator and well counter were showed poor performance status. On the other hand, The quality control of gamma camera and other systems were showed relatively good performance status. The results of precision of dose calibrator and thyroid uptake system was $\pm$1.4%(<$\pm$5%) and chi^2=29.7(>16.92), respectively. It showed that the sensitivity of CeraSPECT$^{TM}$ was higher in center slices compared with the edge slices. After correction of nonuniform sensitivities for patient data, it showed better results compare with prior to correction. System planar sensitivity of Varicam gamma camera was 4.39 CPM/MBq. The observed count rate at 20% loss was 102,407 counts/sec (head 1), 113,427 counts/sec (head 2), when input count rate was 81,926 counts/sec (head 1), 90,741 counts/sec (head 2). The spatial resolution without scatter medium were 8.16 mm of FWHM and 14.85 mm of FWTM. The spatial resolution with scatter medium were 8.87 mm of FWHM and 18.87 mm of FWTM. Conclusion: It is necessary to understand the importance of quality control and to perform quality control of nuclear medicine devices.vices.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.48 no.1
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• pp.16-22
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• 2011

Recently, the use of portable projector expands applications to meeting at fields. Accordingly, the projection is not always guaranteed on white screen, causing some color distortion. Several algorithms have been suggested to correct the projected color on the light colored screen. These have limitation on the use of measurement equipment which can't bring always. In this paper, color correction method using general still camera as convenient measurement equipment is proposed to match the colors between on white and colored screens. A patch containing 9 ramps of each channel are firstly projected on white and colored screens, then captured by the camera, respectively, Next, digital values are obtained by the captured image for each ramp patch on both screens, resulting in different values to the same patch. After that, we check which ramp patch on colored screen has the same digital value on white screen, repeating this procedure for all ramp patches. The difference between corresponding ramp patches reveals the quantity of color shift. Then, color correction matrix is obtained by regression method using matched values. In the experimental results, the proposed method gives better color correction on the objective and subjective evaluation than the previous methods.

• Proceedings of the KSME Conference
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• 2001.06e
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• pp.542-547
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• 2001

A non-intrusive Planar Laser-Induced Fluorescence(PLIF) technique was applied to study the turbulent mixing process in a Rushton turbine reactor. Instantaneous and ensemble averaged concentration fields are obtained by measuring the fluorescence intensity of Rhodamine B tracer excited by a thin Nd:Yag laser sheet illuminating the whole center plane of the stirred tank. The gray level images captured by a 14-bit cooled CCD camera can be transformed to the local concentration values using a calibration matrix. The dye injection point was selected at the tank wall with three quarter height (3/4H) from the tank bottom to observe the mixing characteristics in upper bulk flow region. There exist distinct two time scales: the rapid decay of mean concentration in each region after the dye infusion reflects the large scale mixing while the followed slow decay reveals the small scale mixing. The temporal change of concentration probability functions conjectures the two sequential processes in the batch type mixing. An inactive column of water existed above the impeller disk, in which the fluid rotates with the shaft but is isolated from the mean bulk flow.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.26 no.8
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• pp.1145-1152
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• 2002

A non-intrusive Planar Laser-Induced Fluorescence(PLIF) technique was applied to study the turbulent mixing process in a Rushton turbine reactor. Instantaneous and ensemble averaged concentration fields was obtained by measuring the fluorescence intensity of Rhodamine B tracer excited by a thin Nd:Yag laser sheet illuminating the whole center plane of the stirred tank. The gray level images captured by a 14-bit cooled CCD camera could be transformed to the local concentration values using a calibration matrix. The dye injection point was selected at the tank wall with three quarter. height (3/4H) from the tank bottom to observe the mixing characteristics in upper bulk flow region. There exist distinct two time scales: the rapid decay of mean concentration after the dye infusion reflects the large scale turbulent mixing while the fellowed slow decay reveals the small scale molecular mixing. The temporal change of concentration variance field conjectures the two sequential processes for the batch type mixing. An inactive column of water is existed above the impeller disk, in which the fluid rotates with the shaft but is isolated from the mean bulk flow.

• Journal of the Korean Graphic Arts Communication Society
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• v.25 no.1
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• pp.11-26
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• 2007

In this paper, an experiment was done where the input device used the linear multiple regression and the sRGB color space to perform a color transformation. The output device used the GOG, GOGO and sRGB for the color transformation. After the input device underwent a color transformation, a $3\;{\times}\;20\;size$ matrix was used in a linear multiple regression and the scanner's color representation of scanner was better than a digital still camera's color representation. When using the sRGB color space, the original copy and the output copy had a color difference of 11. Therefore it was more efficient to use the linear multiple regression method than using the sRGB color space. After the input device underwent a color transformation, the additivity of the LCD monitor's R, G and B signal value improved and therefore the error in the linear formula transformation decreased. From this change, the LCD monitor with the GOG model applied to the color transformation became better than LCD monitors with other models applied to the color transformation. Also, the color difference varied more than 11 from the original target in CRT and LCD monitors when a sRGB color transformation was done in restricted conditions.

• Journal of the Korean Graphic Arts Communication Society
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• v.26 no.1
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• pp.51-64
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• 2008

In this paper, an experiment was done where the input(scanner, digital still camera) and monitor(CRT, LCD) device used the linear multiple regression and the GOG (Gain-Offset-Gamma) characterization model to perform a color transformation. Also to color conversion method of the digital printer it used the LUT(Look Up Table), 3dimension linear interpolation and a tetrahedron interpolation method. The results are as follows. From color reappearance of digital printing case of monitor, the XYZ which it converts in linear multiple regression of input device it multiplied the inverse matrix, and then it applies the inverse GOG model and after color converting the patch of the result most which showed color difference below 5 at monitor RGB value. Also, The XYZ which is transmitted from the case input device which is a printer it makes at LAB value to convert an extreme, when the LAB value which is converted calculating the CMY with the LUT and tetrahedral interpolations the color conversion which considers the black quantity was more accurate.

• Journal of Institute of Control, Robotics and Systems
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• v.17 no.2
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• pp.157-163
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• 2011

This paper proposes a hybrid image stabilizing system which uses both optical image stabilizing system based on EKF (Extended Kalman Filter) and digital image stabilization based on SURF (Speeded Up Robust Feature). Though image information is one of the most efficient data for object recognition, it is susceptible to noise which results from internal vibration as well as external factors. The blurred image obtained by the camera mounted on a robot makes it difficult for the robot to recognize its environment. The proposed system estimates shaking angle through EKF based on the information from inclinometer and gyro sensor to stabilize the image. In addition, extracting the feature points around rotation axis using SURF which is robust to change in scale or rotation enhances processing speed by removing unnecessary operations using Hessian matrix. The experimental results using the proposed hybrid system shows its effectiveness in extended frequency range.