• Korean Journal of Agricultural Science
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• v.40 no.3
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• pp.177-182
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• 2013

A genus Camelina has been attracted as a promising oil crop, especially available in drought and marginal conditions. Due to more demands on arable land for bioenergy crops, price of agricultural products has been a challengeable issue. In that respect, development of Camelina crop with higher germination rate and germination energy can be a strategy to secure seedling establishment, nutrient uptake and long vegetative period. In order to be easily available in the field and laboratory conditions, Camelina seed needs to be optimized for its germination temperature. Germination temperature regime was in a range of 8 to $32^{\circ}C$ initially, and consecutively narrowed down to 8 to $20^{\circ}C$. Based on the temperature range, Camelina germinated greater than 96% at $8-16^{\circ}C$ in two weeks after sowing, but germination rate started to decrease at the higher than $24^{\circ}C$ and was significantly low at higher than $32^{\circ}C$. In terms of rapid time to reach the maximum germination rate and greater germination energy, temperature ranged from 12 to $16^{\circ}C$ was found to be desirable for Camelina germination. Although germinationa rate was greater at $16^{\circ}C$, lower temperature close to $12^{\circ}C$ would be favored for the field conditions where greater root growth leading to healthier seedlings and better nutrient or water availability is considerably demanded.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.10a
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• pp.97-97
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• 2017

• Proceedings of the Korean Society of Crop Science Conference
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• 2019.09a
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• pp.49-49
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• 2019

• Proceedings of the Korean Society of Crop Science Conference
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• 2020.06a
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• pp.68-68
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• 2020

• Proceedings of the Korean Society of Crop Science Conference
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• 2020.06a
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• pp.69-69
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• 2020

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.194-194
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• 2017

Camelina (Camelina sativa L.) is a potential bio-energy crop that has short life cycle about 90 days and contains high amount of unsaturated fatty acid which is adequate to bio-diesel production. Enhancing environmental stress tolerance is a main issue to increase not only crop productivity but also big mass production. CsRCI2s (Rare Cold Inducible 2) are cold and salt stress related protein that localized at plasma membrane (PM) and assume to be membrane potential regulation factor. These proteins can be divide into C-terminal tail (CsRCI2D/E/F/G) or no-tail group (CsRCI2A/B/C/H). However, function of CsRCI2s are less understood. In this study, physiological responses and functional characterization of CsRCI2s of Camelina under salt stress were analyzed. Full-length CsRCI2s (A/B/E/F) and CsPIP2;1 sequences were confirmed from Camelina genome browser. Physiological investigations were carried out using one- or four-week-old Camelina under NaCl stress with dose and time dependent manner. Transcriptional changes of CsRCI2A/B/E/F and CsPIP2;1 were determined using qRT-PCR in one-week-old Camelina seedlings treated with NaCl. Translational changes of CsRCI2E and CsPIP2;1 were confirmed with western-blot using the antibodies. Water transport activity and membrane potential measurement were observed by cRNA injected Xenopus laevis oocyte. As results, root growth rate and physiological parameters such as stomatal conductance, chlorophyll fluorescence, and electrolyte leakage showed significant inhibition in 100 and 150 mM NaCl. Transcriptional level of CsPIP2;1 did not changed but CsRCI2s were significantly increased by NaCl concentration, however, no-tail type CsRCI2A and CsRCI2B increased earlier than tail type CsRCI2E and CsRCI2F. Translational changes of CsPIP2;1 was constitutively maintained under NaCl stress. But, accumulation of CsRCI2E significantly increased by NaCl stress. CsPIP2;1 and CsRCI2A/B/E/F co-expressed Xenopus laevis oocyte showed decreased water transport activity as 61.84, 60.30, 62.91 and 76.51 % at CsRCI2A, CsRCI2B, CsRCI2E and CsRCI2F co-expression when compare with single expression of CsPIP2;1, respectively. Moreover, oocyte membrane potential was significantly hyperpolarized by co-expression of CsRCI2s. However, higher hyperpolarized level was observed in tail-type CsRCI2E and CsRCI2F than others, especially, CsRCI2E showed highest level. It means transport of $Na^+$ ion into cell is negatively regulated by expression of CsRCI2s, and, function of C-terminal tail is might be related with $Na^+$ ion influx. In conclusion, accumulation of NaCl-induced CsRCI2 proteins are related with $Na^+$ ion exclusion and prevent water loss by CsPIP2;1 under NaCl stress.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.1
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• pp.85-90
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• 2015

The objective of this study was comparison of seed yields according to different seeding rates, seeding time and application of chemical fertilizations in Yanji region of China. The experiment was conducted on commercial farmland at Yanji region in 2013. All the experimental fields were designed following randomized block design with 3 replicates. The plant spacing was applied as $65{\times}1cm$ distance. To check the effect of seeding rate, two different seeding rate, 0.2 kg/0.1ha and 0.5 kg/0.1ha were applied. The higher seed yield was observed in low seeding rate (0.2 kg/0.1ha). The application of fertilizer (mixture of N and P) showed different results following different experimental fields. However, there were little positive effects following fertilizer application into commercial farmland. When we compare seed yields between two different seeding dates, $4^{th}$ May and $29^{th}$ May, the earlier seeding date ($4^{th}$ May) showed higher seed yields. In considering these results, low seeding rate and early seeding time is important for getting high camelina seed yields.