• 제목/요약/키워드: Camelina Sativa

검색결과 19건 처리시간 0.037초

Expression of CsRCI2s by NaCl stress reduces water and sodium ion permeation through CsPIP2;1 in Camelina sativa L.

  • Kim, Hyun-Sung;Lim, Hyun-Gyu;Ahn, Sung-Ju
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.194-194
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    • 2017

  • Camelina (Camelina sativa L.) is a potential bio-energy crop that has short life cycle about 90 days and contains high amount of unsaturated fatty acid which is adequate to bio-diesel production. Enhancing environmental stress tolerance is a main issue to increase not only crop productivity but also big mass production. CsRCI2s (Rare Cold Inducible 2) are cold and salt stress related protein that localized at plasma membrane (PM) and assume to be membrane potential regulation factor. These proteins can be divide into C-terminal tail (CsRCI2D/E/F/G) or no-tail group (CsRCI2A/B/C/H). However, function of CsRCI2s are less understood. In this study, physiological responses and functional characterization of CsRCI2s of Camelina under salt stress were analyzed. Full-length CsRCI2s (A/B/E/F) and CsPIP2;1 sequences were confirmed from Camelina genome browser. Physiological investigations were carried out using one- or four-week-old Camelina under NaCl stress with dose and time dependent manner. Transcriptional changes of CsRCI2A/B/E/F and CsPIP2;1 were determined using qRT-PCR in one-week-old Camelina seedlings treated with NaCl. Translational changes of CsRCI2E and CsPIP2;1 were confirmed with western-blot using the antibodies. Water transport activity and membrane potential measurement were observed by cRNA injected Xenopus laevis oocyte. As results, root growth rate and physiological parameters such as stomatal conductance, chlorophyll fluorescence, and electrolyte leakage showed significant inhibition in 100 and 150 mM NaCl. Transcriptional level of CsPIP2;1 did not changed but CsRCI2s were significantly increased by NaCl concentration, however, no-tail type CsRCI2A and CsRCI2B increased earlier than tail type CsRCI2E and CsRCI2F. Translational changes of CsPIP2;1 was constitutively maintained under NaCl stress. But, accumulation of CsRCI2E significantly increased by NaCl stress. CsPIP2;1 and CsRCI2A/B/E/F co-expressed Xenopus laevis oocyte showed decreased water transport activity as 61.84, 60.30, 62.91 and 76.51 % at CsRCI2A, CsRCI2B, CsRCI2E and CsRCI2F co-expression when compare with single expression of CsPIP2;1, respectively. Moreover, oocyte membrane potential was significantly hyperpolarized by co-expression of CsRCI2s. However, higher hyperpolarized level was observed in tail-type CsRCI2E and CsRCI2F than others, especially, CsRCI2E showed highest level. It means transport of $Na^+$ ion into cell is negatively regulated by expression of CsRCI2s, and, function of C-terminal tail is might be related with $Na^+$ ion influx. In conclusion, accumulation of NaCl-induced CsRCI2 proteins are related with $Na^+$ ion exclusion and prevent water loss by CsPIP2;1 under NaCl stress.

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파종량과 비료 시용이 봄 재배용 카멜리나 종자 생산량에 미치는 영향 (The effect of Camelina sativa (L.) cv. Crantz Seed Yield according to Seeding Rate and Chemical Fertilization)

  • 박민우;최충원;오명근;이상협
    • 한국작물학회지
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    • 제60권1호
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    • pp.85-90
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    • 2015

  • 바이오 디젤용 원료작물인 카멜리나를 중국 지역에서 대량 상용재배 하기 위해서 길림성 연길 지역에서 재식 밀도, 비료 시비, 파종 시기 등이 종자 생산량에 미치는 영향을 실험하였다. 재식 밀도와 증수량의 상관 관계를 알아보기 위해서 단위면적당 다른 두 가지의 파종량을 적용했다. 0.1 ha 당 카멜리나 종자 0.23 kg을 파종한 시험구가 0.56 kg을 파종한 시험구보다 60.6 g이 증대되는 결과를 보였다. 또한 비료 시비량과 증수량의 상관관계를 알아보기 위해서 0.1ha당 질소 1.97 kg과 인 12.3 kg을 시용한 시험구, 질소 2.8 kg과 인 17.5 kg을 시용한 시험구, 그리고 무시비구를 이용해서 증수량을 분석한 결과 재배지역에 따라 상이한 결과를 보였다. 다른 실험 결과와 비교하면 상당히 낮은 시비량에도 증수량이 포화되는 결과를 보이는 것으로 봐서 시험구 포장 자체가 상당한 양의 비료 성분을 포함하는 것으로 추정되며 통상적인 농경지 재배에서는 추가적인 시비 효과가 크지 않을 것으로 여겨진다. 파종 시기에 따른 종자수확량은 동일한 생육 기간을 거치더라도 5월 4일에 파종을 한 후 수확을 하는 것이 5월 29일 파종한 것보다 높은 생산량을 보였다. 이 결과는 빠른 파종 시기가 종자 생산량에 긍정적인 영향을 미친다는 것을 의미하므로 최대한 파종 시기를 앞당기는 것이 종자의 생산량을 늘리는데 도움이 될 것이라고 판단 된다.

  • https://doi.org/10.7740/kjcs.2014.60.1.085 인용 PDF KSCI

Study on hydroxy fatty acid contents changes and physiological responses under abiotic stresses in transgenic Camelina

  • Kim, Hyun-Sung;Lee, Hyun-Sook;Lim, Hyun-Gyu;Park, Won;Kim, Hyun-Uk;Lee, Kyeong-Ryeol;Ahn, Sung-Ju
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.191-191
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    • 2017

  • Hydroxy fatty acid (HFA) is an important industrial resource that known to be extracted from seeds of Castor or Lesquerella. However, mass production of HFA from those crops are difficult because of their behavior or life cycle. In this study, we applied HFA synthesis related gene FAH12, RcPDAT1, RcLPCAT, RcDGAT2, and RcPDCT on bioenergy crop Camelina sativa. Furthermore, we determined NaCl or cold stress tolerance changes of transgenic Camelina. RcFAH12, RcPDAT1, RcLPCAT, RcDGAT2, and RcPDCT genes were cloned into multigene expression vector which is engineered with seed specific promoter of FAE1 or Napin. Combination of HFA genes multi-expression vector constructs were divided into Set3 (RcFAH12, RcPDAT1-2, RcLPCAT), Set4 (RcFAH12, RcDGAT2, RCPDAT1-2, RcLPCAT), and Set5 (RcFAH12, RcDGAT2, RCPDAT1-2, RcLPCAT, RcPDCT). Transgenic HFA synthesis Camelina plants were generated using agrobacterium-mediated vacuum infiltration system. Results of fatty acid composition of T1 transgenic Camelina seeds analyzed by GC-MS showed 9.5, 9.0, and 13.6 % of HFA contents in Set3#6, Set4#8, and Set5#10, respectively. Therefore, seeds of T2 generation were harvest from Set5#10 which is shown highest HFA contents, and, 17.7, 8.1 and 10.5 % of HFA contents were determined in Set5#10-5, Set5#10-8, and Set#10-10, respectively. However, 7.7% of C18:2 and 22.3 % of C18:3 among unsaturated fatty acids were decreased in Set5#10-5 than WT. Meanwhile, we confirmed abiotic stress responses in T2 transgenic Camelina Set5#10-5 and Set5#10-10 under 0, 100, 150, and 200 mM NaCl or 25, 15, and $10^{\circ}C$ temperature for 5 weeks. Both Set5#10-5 and Set5#10-10 showed lower growth in height than WT in control and NaCl condition. Growth of leaf length and width were similar in WT and Set5#10-10 but lower in Set5#10-5 under NaCl stress. Number of opened flowers showed that both transgenic Camelina were lower than WT under normal condition. But, WT and Set5#10-10 showed similar opened flower number in 100 and 200 mM NaCl. In cold stress, 15 and $10^{\circ}C$ treatment for 5 weeks did not showed significant changes in between WT and both transgenic lines even they showed different growth rate in control condition. Taken together, growth and development are delayed by expression of exogenous HFA related genes in transgenic lines but relative abiotic stress sensitivity is similar with WT. In conclusion, reduced C18:2 or C18:3 fatty acid composition of seed by HFA synthesis is resulted from lack of resource supplement for development at seedling stage but it is not affect NaCl and cold stress tolerance.

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