• Journal of Plant Biology
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• 제38권2호
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• pp.173-180
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• 1995

We investigated levels and distribution of genetic variation in Korean populations of Calystegia soldanella and C. japonica, clonally reproducing herbaceous perennials. Calystegia soldanella is one ofecologically important beach plants growing only on sand and beach dunes in Europe, East Asia, the Pacific Islands, and the west coast of North America. In contrast, C. japonica usually grows on small mounds of paddy fields, roadsides, and waste places with patchy distribution. Starch gel electrophoresis was conducted on leaves collected from 13 populations of C. soldanella and eight populations of C. japonica. The levels of genetic variation of the two species are very comparable; means of expected heterozygosity (Hep) were 0.100 and 0.099 for C. soldanella and C. japonica, respectively. These values were also very similar to those for species with similar life-history and ecological traits. However, the proportion of total genetic diversity partitioned among populations (GST) of C. soldanella (0.146) was considerably lower than that of C. japonica (0.383). In addition, means of Nei's genetic identity (Ⅰ) for C. soldanella and C. japonica were 0.985 and 0.900, respectively, which supports a restricted gene flow resulting from obligate clonal reproduction of C. japonica. Significant differences in allele frequency were detected among populations at eight and nine of nine polymorphic loci for C. soldanella and C. japonica (P<0.01), respecitvely. Considering the ecological importance of C. soldanella, the isolated beach populations coupled with present destruction of natural habitats of the species may result in erosion of genetic diversity in the near future. In this respect, conservation efforts should be focused on those populations that currently maintain the most genetic diversity such as those populations in the eastern and southeastern Korean Peninsula and Hamduck Beach, Cheju Island.

• Journal of Microbiology and Biotechnology
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• 제34권5호
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• pp.1092-1100
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• 2024

The global elderly population, aged 65 and over, reached approximately 10% in 2020, and this proportion is expected to continue rising. Therefore, the prevalence of neurodegenerative diseases such as Parkinson's disease (PD), which are characterized by declining memory capabilities, is anticipated to increase. In a previous study, we successfully restored the diminished memory capabilities in a fruit fly model of PD by administering an omija extract. To identify functional ingredients that can enhance memory akin to the effects of the omija extract, we conducted screenings by administering halophyte extracts to the PD model. Halophytes are plants that thrive in high-salt environments, and given Korea's geographic proximity to the sea on three sides, it serves as an optimal hub for the utilization of these plants. Upon examining the effects of the oral administration of 12 halophyte extracts, Salicornia herbacea and Calystegia soldanella emerged as potential candidates for ameliorating memory loss in PD model flies. Moreover, our findings suggested that C. soldanella, but not S. herbacea, can mitigate oxidative stress in DJ-1β mutants.

• 한국환경과학회지
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• 제22권2호
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• pp.187-193
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• 2013

Dry matter allocation characteristics of Calystegia soldanella, grown in pots, was analysed to assess its plasticity in response to water-stressed conditions. As water was withheld leaf water potential between the two watering treatments was similar during the first 6 days, followed by a rapid decrease in water-stressed plants. The minimum leaf water potential was -1.50 MPa on day 15 and the maximum leaf water potential was about -0.5 MPa on day 0 in water-stressed plants. In well-watered plants leaf water potential was maintained almost consistently throughout the experiment. There was no significant difference in plant dry weight between the two watering treatments for 9 days after the start of experiment and that was remarkably increased thereafter, compared with that remained without any increase in water-stressed plants. In dry mass partitioning, however, the water-stressed plants showed a great plasticity, showing that there were 1.81, 1.35 and 0.81 times increase in root, stem and leaf, respectively. Dry mass partitioning in well-watered plants varied from 2% to 5%. The difference of dry mass partitioning between the two watering treatments was reflected in leaf mass per unit area (LMA) and root/shoot (R/S) ratio. LMA in water-stressed plants was lower than that in well-watered plants, while R/S ratio in water-stressed plants was higher in well-watered plants. This means that the water-stressed plants reduced its leaf area and increased dry mass partitioning into root and stem during the progress of soil drying. These results indicate that Calystegia soldanella inhabiting in sand dune cope with water stress with high plasticity which can adjust its dry mass partitioning according to soil water conditions.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2021년도 춘계학술대회
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• pp.37-37
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• 2021

Calystegia soldanella L. is a perennial herbaceous halophyte belonging to the convolvulaceae family, which mainly grows in coastal sand dunes in Korea. Shoots and rhizomes are edible, and roots called 'Hyoseon Chogeun' are known to have medicinal effects such as antipyretic, sterilization, and diuretic. In addition, physiological activities of antioxidant, anti-inflammatory, antiviral, antifungal and PTP-1B (protein tyrosine phosphate-1B) inhibition have been reported. In this study, in vitro induction cell lines of C. soldanella L. collected from the coastal sand dunes in Jeju island was redifferentiated into adventitious roots that can be used as medicinal resources. Also the biomass of mass-proliferated adventitious roots using a bioreactor were evaluated. Plants of C. soldanella L. were collected from the crevice of the seashore in the coastal area of Taeheung 2-ri, Namwon-eup, Seogwipo-si. Then, it was separated into leaves, stems, rhizomes, and roots, and surface sterilized with 70% ethyl alcohol and 2% NaOCl (sodium hypochlorite). After washing with sterilized water, each organ section was cultured in Hormone-free MS medium (Murashige & Skoog Medium). As a result, the induction response rates were evaluated at 85% and 55%, respectively, in terms of callus formation and shoot generation in the rhizome segment. In the case of the adventitious roots morphological characteristics induced by single-use treatment of auxin-based plant growth regulators IBA and NAA from redifferentiated shoots were compared. Most efficient adventitious root culture method as a rooting rate, number, length, and biomass proliferation in the bioreactor system was confirmed when treated by culturing in MS salts, Sucrose 30 g·L^-1, and IBA 1mg·L^-1 for 4 weeks. In this study, the medium composition and culture period were confirmed using a bioreactor system to mass-proliferate adventitious roots derived from C. soldanella L. in Jeju island. Also this adventitious root line developed a new medicinal material could increase value of the bio-industry ingredient through quantitative and qualitative screening of phyto-bioactive compounds.

• 한국약용작물학회지
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• 제14권6호
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• pp.347-353
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• 2006

This study was conducted to evaluate the vegetation structure and ordination of the Peucedanum japonicum Thunb. community by the Phytosociological method, floristic composition table on east coast of South Korea. The vegetation of Peucedanum japonicum Thunb. community was classified into 2 communities such as the Aster Spathulifolius community and the calystegia soldanella-Artemisia princeps community. Vegetation of the Peucedanum japonicum Thunb. community with the Aster spathulifolius community was shown southward, whereas vegetation of Peucedanum japonicum Thunb. community with the calystegia soldanella-Artemisia princeps community was shown northward from the base line of Pohang at North latitude of 36" 05'. Accordingly, the Peucedanum japonicum Thunb. community was grown commonly with the Aster spathulifolius community southward of the base line of Pohang, whereas it was grown commonly with the calystegia soldanella-Artemisia princeps community northward on the east coast. All environmental conditions of habitat taken together, the optimum habitat of Peucedanum japonicum Thunb. was at Ulsan geographically and the middle region of sea cliff topography, and was suitable for alkali. sandyloam.

• Journal of Plant Biology
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• 제27권1호
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• pp.33-41
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• 1984

The relationships and character variations on 5 taxa of Calystegia were examined by sluster analysis and principal component analysis. Thirteen Calystegia population samples from the middle part of Korea were observed. Although minor differences were noted, essentially similar results were obtained from the phenograms by UPGMA, UPGMC and Ward's clustering methods, and these results were in accordance with those obtained from the ordination plots by principal component analysis. C. soldanella is distantly connected with the other taxa mainly because of its morphologically different leaf organs. Based on the difference on the first principal component, C. hederacae is kept apart from the rest 3 taxa. In the relationships among C. japonica, C. sepium var. americana and C. davurica, mivor differences were obtained from the 3 clustering methods. As to the character variations among different populations within a taxon, they are slight in C. soldanella and C. sepium var. americana, but remarkable in C. hederacae and C. davurica.

• Journal of Ecology and Environment
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• 제36권4호
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• pp.255-265
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• 2013

This study was conducted to determine the extent of drought resistance based on physiological responses of Calystegia soldanella under water deficit. In order to investigate the changes of plant growth, stomatal density, photosynthesis, chlorophyll fluorescence, the contents of chlorophyll and carotenoid, osmolality, total ion contents, the contents of carbohydrate and proline, C. soldanella was grown under well watered and drought stressed conditions for 12 days. In this study, water-deficit resulted in remarkable growth inhibition of C. soldanella. The effect of water-deficit on plant growth was associated with low osmotic potential of soil. On day 12 after drought treatment, dry weight, relative water contents, number and area of leaves and stem length were lower than those of control. The stomatal conductance and net photosynthetic rate were significantly reduced in water stressed plant to regulate inner water contents and $CO_2$ exchange through the stomatal pore. Chlorophyll fluorescence and chlorophyll contents were not different in comparison with the control, indicating that the efficiency of photosystem II was not affected by drought stress. This results could be explained that water-deficit in C. soldanella limits the photosynthetic rate and reduces the plant's ability to convert energy to biomass. A significant increase in total ion contents and osmolality was observed on day 7 and day 12. Accumulation of proline in leaves is associated with the osmotic adjustment in C. soldanella to soil water-deficit. Consequently, this increase in osmolality in water stressed plant can be a result in the increase of ion contents and proline.

• Journal of Ecology and Environment
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• 제30권2호
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• pp.135-142
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• 2007

We used the Braun-Blanquet method to study coastal dune vegetation of South Korea. Coastal vegetation was monitored in thirty sites from April 2004 to September 2005. Seventeen plant communities were classified into five groups as follows: A. Two associations of herbaceous sand dune plants in Salsoletea komarovii Ohba, Miyawaki et Tx. 1973 (Calystegia soldanella community, Lathyrus japonicus-Calystegia soldanella community), B. Twelve associations of herbaceous perennial sand dune plants in Glehnietea littoralis Ohba, Miyawaki et Tx. 1973 (Carex pumila community, Elymus mollis community, Vitex rotundifolia-Elymus mollis community, Ixeris repens community, Elymus mollis-Ixeris repens community, Lathyrus japonicus community, Messershmidia sibirica community, Glehnia littoralis community, Carex kobomugi community, Calystegia solda-nella-Carex kobomugi community, Ishaemum anthephoroides community, Zoysia macrostachya community), C. One association of shrubby perennial sand dune plant in Viticetea rotundifoliae Ohba, Miyawaki et Tx. 1973 (Vitex rotundifolia community), D. One association of shrubby perennial sand dune plant in Rosetea multiflorae Ohba, Miyawaki et Tx. 1973 (Rosa rugosa community), E. The naturalized community (Diodia teres community).

• 생명과학회지
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• 제29권3호
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• pp.287-294
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• 2019

갯메꽃은 해안 사구에 분포하며 식물로 높은 환경 적응력을 가지고 있으며 갯메꽃은 항산화, 해열, 살균, 이뇨작용 등에 효과가 있는 것으로 알려져 있다. 본 연구에서는 암전이 과정에서 중요한 역할을 한다고 알려진 matrix metalloproteinases (MMPs)의 일종인 MMP-2와 MMP-9의 활성에 대한 갯메꽃의 억제효과를 methylene chloride (MC) 및 methanol (MeOH) 추출물과 조추출물, 유기용매 분획물을 시료로 하여 인체 섬유육종 HT-1080 세포를 이용하여 ELISA (Enzyme-linked immunosorbent), gelatin zymography, Wound healing assay, RT-PCR, western blot 실험을 통해 조사하였다. 갯메꽃의 MC 및 MeOH 추출물, 조추출물과 4가지 유기용매 분획물이 HT-1080 세포에서 MMP-2와 MMP-9 생성 억제 활성을 나타냄을 확인하였다. 그 중에서도 n-hexane, 85% aq.MeOH 분획물이 전반적으로 MMP-2와 MMP-9에 대한 높은 억제활성을 보였다. 이들 결과로부터 85% aq.MeOH과 n-hexane 분획물에 MMP 억제 활성이 높은 물질이 존재할 것으로 추측되어지며, 갯메꽃 추출물 및 분획물이 암침윤 및 전이를 억제하는 효과적인 항암소재로서의 가능성이 있음을 제시한다. 이에 본 연구팀은 85% aq.MeOH과 n-hexane 분획물에 있는 활성물질을 분리하기 위한 연구를 추가적으로 수행할 계획이다.

• 생명과학회지
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• 제20권9호
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• pp.1373-1377
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• 2010

해안사구식물은 다양한 미생물과 외생균근과 내생균근의 도움으로 효과적으로 생존할 수 있다. Penicillium citrinum KACC43900를 이용하여, 해안사구식물인 갯메꽃과 갯쇠보리에 대해 생장 촉진효과를 조사하였으며, 지상부길이, 식물체길이 그리고 식물체건량을 분석하였다. P. citrinum KACC43900 배양액을 20일간 처리 시, 지상부길이는 16.30 cm와 10.56 cm이었으며, 대조구는 8.60 cm과 8.12 cm인 것을 확인 하였다. 마찬가지로 P. citrinum KACC43900 배양액을 20일간 처리 시, 갯메꽃과 갯쇠보리의 식물체길이는 24.06 cm와 17.06 cm인 것을 확인 하였고, 대조구는 14.90 cm과 14.80 cm인 것을 확인 하였다. 갯메꽃의 식물체건량은 배양여과액 처리의 경우, 0.163g이었고 대조구가 0.123 g이었다. 그리고 갯쇠보리의 식물체건량은 배양여과액 처리의 경우, 0.032 g이었으며 대조구가 0.027 g이었다. 갯메꽃과 갯쇠보리의 지상부길이는 P. citrinum KACC43900의 배양여과액에 의해 89.54%와 29.60% 촉진되었다.