• Journal of Acupuncture Research
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• v.19 no.2
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• pp.164-176
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• 2002

Objective : This research carried out to find out the effective efficiency of acupuncture at ST36 GB39 and herbal-acupuncture using different concentrational Uris Fel Bovis Calculus on adjuvant arthritis in rats. Method : In this study, I do acupuncture treatment and herbal-acupuncture in ST36 GB39 by solution that diluted to fivefold and tenfold with combined solution of Bovis Calcuclus and Ursi Fel at 10:1. And then I observed the change of inhibitory rate of paw edema, body weight, hematologic assay, histologic assay. Result : 1. In Adjuvant Arthritis in rats, the herbal-acupuncture group 1 is more efficienct then acupuncture group. 2. In histologic assay of knee joint, synovial cell and fibroblast markedly activated in herbal-acupuncture group. 3. The albumin level in serum more markedly increased in herbal-acupuncture group 2 than the control group. 4. The paw edema significantly decreased in acupuncture group and herbal-acupuncture group 2 compared with the control group. 5. The body weight significantly increased in acupuncture group and herbal-acupuncture group 2 compared with the control group. Conclusion : In these results, The herbal-acupuncture using Ursi Fel, Bovis Calculus, suppress inflammation of Adjuvant Arthritis, and decrease markedly the count of WBC, and increase the albumin level in serum, and have an influence on Adjuvant Arthritis with activating of synovial cell, fibroblast. And these were more effective than the others, and then needed consistent study of its mechanism.

• Journal of Pharmacopuncture
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• v.15 no.4
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• pp.25-31
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• 2012

Objectives: This study was designed to investigate the effect of four pharmacopuncture drugs (scolopendrid, Calculus Bovis-Fel Uris-Moschus (BUM), bee venom 25%, and sweet bee venom 10%) on the cerebral hemodynamics, including changes in the regional cerebral blood flow (rCBF) and in the mean arterial blood pressure (MABP). Methods: The changes in the rCBF and the MABP were determined by using a laser-Doppler flowmeter and a pressure transducer, respectively. Results: Scolopendrid (0.3 ml, 1 ml/kg) caused no significant changes in the rCBF and the MABP, whereas BUM (0.3 ml, 1 ml/kg) decreased the rCBF and the MABP, bee venom 25% (0.3 ml, 1 ml/kg) increased the rCBF and lowered the MABP, and sweet bee venom 10% (0.3 ml, 1 ml/kg) increased the rCBF and had no significant effect on the MABP. Conclusions: The rCBF and the MABP were influenced differently by the administration of various pharmacopunctures. Further studies are needed to elucidate the underlying mechanism.

• Journal of Acupuncture Research
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• v.28 no.4
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• pp.105-110
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• 2011

Objectives : The purpose of this study was to evaluate the effectiveness of Calculus Bovis Fel Ursi Moschus(BUM) pharmacopuncture on the treatment of acute low back pain patients. Methods : The subjects in this study were 30 patients with acute low back pain who were treated in Dept. of Acupuncture and Moxibustion, Jein Oriental Medicine Hospital from November 1, 2010 to June 30, 2011. These patients were randomly divided into two groups. In one group(control group), patients were treated with acupuncture. And in the other group(experimental group), patients were treated with both acupuncture and BUM pharmacopuncture. For evaluating change of pain, visual analog scale(VAS) and Oswestry disability index(ODI) were checked before and after. Results : Each group had significant decrease in VAS and ODI after each treatment. Experimental group had more significant decrease in VAS after each treatment than control group. Conclusions : We suggest that cotreatment of acupuncture and BUM pharmacopuncture on acute low back pain patients can be recommended as a useful therapy.

• The Journal of Korean Medicine
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• v.17 no.2 s.32
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• pp.251-263
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• 1996

In order to investigate experimentally that effect of Moschus, Bovis Calculus, Ursi Fel aqua-axupuncture on acutely damaged liver of rats induced by radix aconiti. The author admininisterated aqua-acupuncture according to method of manufacture stimulation to corresponding points, Kan-su$(BL_8)$ and Ki-mun($LR_{14}$), and carried out hematological, serological examination and electromicroscopical obseration. WBC level was decreased significantly in the experimental groups at 24 and 48 hours as compared with the control group. ${\gamma}-GTP$ level was decreased significantly in the experimental groups at 24 and 48 hours as compared with the control group. GPT level was decreased significantly in the experimental groups at 24 and 96 hours as compared with the control group, but GOT level did not reeveal any statistical difference. At 24-hours group, the cell organelles of the hepatic cells are destructed. The cisternae of the smooth endoplasmic reticulum are particulary dilated in the 24-hours control group compared with experimental group. At 48-hours group, the destructed cell organelles are recovered in the experimental group compared with control group. At 96-hours group, the cell organelles of hepatic cells are showing feture of normal group. According to the above findings, it is considered that Moschus, Bovis Calculus, Ursi Fel aqua-acupuncture has effects of recovery of acutely damaged liver.

• Journal of Pharmacopuncture
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• v.13 no.2
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• pp.101-110
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• 2010

Objectives : This experimental study was performed to investigate the effect of Fel Ursi & Bovis Calculus pharmacopuncture solution(FUBCPS) manufactured by using alcohol/water extraction method for identify ing the use of it as eyedrops. Methods: FUBCPS was manufactured by using alcohol/water extraction method. Measure the size of inhibition zone and MIC(Minimum Inhibition Concentration) after administering FUBCPS on bacterial species. Staphylococcus aureus. Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum and Candida albicans, which cause keratitis. Administering cravit(Levonoxacin medicine) on bacterial species also performed to compare the anti-bacterial potency of this material, measured by using the size of inhibition zone Results : After administering FUBCPS on bacterial species(Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, Candida albicans). there was no response to MIC and there was no anti -bacterial potency also. Conclusions : This study suggests that FUBCPS dose not have anti-bacterial effects on bacterial species which cause Keratitis. These study result recommends that we need to research more on herbal medicines of eye drop which have anti-bacterial effects on keratitis

• Applied Microscopy
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• v.27 no.3
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• pp.243-255
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• 1997

To investigate detoxification effects of aqua-acupuncture; Bovis Calculus and Susi Fei extract against the toxicity of aconitine were studied. We examined the GOT activities, GPT activities, $\gamma-GPT$ activites, ALP activities and ultrastructural changes of hepatocytes in rats. The GOT activities were decreased in the aqua-acupuncture group compared with control at the 48-hours and 96-hours. The $\gamma-GTP$ and ALP activities were decreased in the aqua-acpuncture group compared with control at 96-hours. We observed the ultrastructural changes of hepatocytes compared the detoxification effects of acua-acupuncture with toxicity of aconitine by transmission electron microscope. In the 24-hours group, the cisternae of rough and smooth endoplasmic reticulum hepatocytes are dilated more or less. In the 48-hours group, the nuclear envelope are irregured, and chromatins of nucleus are condensed. The cisternae of rough endoplasmic reticulum are dilated/branched. In the 96-hours group, the rough and smooth endoplasmic reticulum are well developed in aqua-acpuncture group compared with control. These results suggest that acua-acpuncture groups of the Bovis calculus and Susi fei extract showed detoxification effects against cytotoxicity of aconitine in the hepatocyte of rats.

• The Journal of Internal Korean Medicine
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• v.19 no.1
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• pp.318-328
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• 1998

The aqua-acupuncture blended with liquid medicine and acupuncture is known to be effective in treating various medical disorders. This study used gallbladders of bear (WoongDam, WD, Ursus arctos Linne) and pig(JeoDam, JD, Sus scrofa domestica Brisson) plus the stones from the ox gallbladder(WooHwang, WH, Bos taurus var, domesticus Gmelin) to produce the liquid medicine. For the acupuncture, acupoint of ST -36 which is located one finger breadth from the anterior crest of the tibia was selected. There were a couple of purposes in this experiment. First, the relationship between aqua-acupuncture and gastric activity was scrutinized. Secondly, the possibility of pig gallbladder as an alternative organ to bear gallbladder for making liquid medicine was evaluated. Thirdly, different injection concentration ratio of liquid medicine was tried to figure out the optimal dosage for the aqua-acupuncture. The data of the present results indicated that the mixtures of WD + WH and JD + WH have a similar hypotonic effect on the stomach. These findings illustrated that aqua-acupuncture or hydroacupuncture showed inhibitory effect on the stomach in addition to its well-known prophylactic value. Also the possible alternative medical organ of pig gallbladder was checked out since two mixtures illustrated very similar trends in suppressing the gastric activity. The injection concentration ratio was not very different between two mixtures in terms of gastric motility except a couple of ratio. In conclusion, the aqua-acupuncture is a promising way to control the gastric activity for better medical purposes and the gallbladder of pig was assessed as a prospective one for making a liquid medicine.

• Journal of Pharmacopuncture
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• v.1 no.1
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• pp.35-52
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• 1997

In order to investigate experimentally that Bovis Calculus, Ursi Feli Aqua-acupuncture(BUA) have an effect on Adjuvant Arthritis in rats, the author inserted BUA at points corresponding with Chok-samni(ST36) and T'aegye(KI3), and observed an inhibitory rate of edema and pain, variations of White blood cell(WBC), Red blood cell(RBC), Hemoglobin(Hb) in blood. The author also observed the histological changes of joint tissue. The results were as follows : 1. The BUA group during the 6th and 9th were decreased with statistical significance in inhibitory rate of paw edema as compared with the control group. 2. The BUA group during the 3rd and 9th day were decreased with statistical significance in inhibitory rate of pain as compared with the control group. 3. The BUA group during the 3rd, 6th and 9th day were decreased with statistical significance in blood WBC as compared with the control group. The blood RBC and Hb didn't have statistical significance. 4. According to the histological studies, the synovial cells were necrotized at the 3rd, 6th and 9th day control group, but some synovial cells were necrotized at the 3rd day BUA group. The synovial cells of the the 6th and 9th day BUA group were recovered more than that of the 3th day group.

• Journal of Acupuncture Research
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• v.24 no.2
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• pp.221-229
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• 2007

Oxidative damage to DNA, proteins, lipids and other molecules may contribute to the development of neurodegenerative disease. This study was designed to find out whether Ursi Fel ${\cdot}$ Bovis Calculus Herbal-Acupuncture Solution(UBHAS) can scavenge nitric oxide(NO) or not. NO concentration was estimated after treatment with Vit. C or 1, 10, $100{\mu}g/m{\ell}$ of UBHAS. There was no significant scavenging effect of UBHAS on NO in 1, $100{\mu}g/m{\ell}$ group. while significant scavenging in $l0{\mu}g/m{\ell}$ group after 2hrs. UBHAS showed significant scavenging effects on NO in 1, 10, $100{\mu}g/m{\ell}$ group after 6hrs. There was a significant scavenging effect in 10, $100{\mu}g/m{\ell}$ group. even though no significant in $1{\mu}g/m{\ell}$ group after 12, 24 hrs. This study showed that UBHAS might be used for aging related disease. These results suggest that UBHAS has scavenging effect on NO. however, further studies are required to investigate the antioxidative effects of it.

• Journal of Pharmacopuncture
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• v.16 no.4
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• pp.30-35
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• 2013

Objectives: This study was designed to investigate the effects of Calculus Bovis-Fel Uris-Moschus pharmacopuncture (BUM) on the regional cerebral blood flow (rCBF) and the mean arterial blood pressure (MABP) in normal and cerebral ischemic rats and to investigate a possible pathway involved in the effects of BUM. Methods: The changes in the rCBF and the MABP following BUM into Fengfu (GV16) were determined by using a laser-Doppler flow meter and a pressure transducer, respectively. Results: BUM significantly increased the rCBF and decreased the MABP in normal rats in a dose-dependent manner. The effect on the rCBF was significantly inhibited by pretreatment with methylene blue (0.01 mg/kg, intraperitoneal), an inhibitor of guanylate cyclase, but was not affected by pretreatment with indomethacin (1 mg/kg, intraperitoneal), an inhibitor of cyclooxygenase. The BUM-induced decrease of the MABP was changed neither by methylene blue nor by indomethacin pretreatment. In the cerebral ischemic rats, the rCBF was stably increased upon cerebral reperfusion in the BUM group in contrast to the rapid and marked increase in the control group. Conclusion: This study demonstrated that BUM into Fengbu (GV16) increased the rCBF in a dose-dependent manner in the normal state; furthermore, it improved the stability of the rCBF in the ischemic state upon reperfusion. Also, the effects of BUM on the rCBF were attenuated by inhibition of guanylate cyclase, suggesting that the effects involved the guanylate cyclase pathway.