• Korean Journal of Food Science and Technology
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• v.20 no.3
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• pp.293-302
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• 1988

This study was performed to identify detailed informations on the nonvolatile flavor of Chinese quince fruits, Chaenomeles sinensis koehne. About 72% of the free amino acids were shown to be valine, asparagine, ${\gamma}-aminobutyric\;acid$, aspartic acid and serine. Arginine, tyrosine, methionine and tryptophan were not present. Glutamic acid and glutamine as a amino acid for peptides were the major components, whereas cysteic acid, methionine sulfone and tryptophan were not detected. The nucleotides attained were composed of cytosine, uridine-5'-monophosphate and cytidine-5'-monophosphate, and these were proved to be a very small quantity. Guanosine-5'-monophosphate, inosine-5'-monophosphate and adenosine-5'-monophosphate were not present. The major sugars were shown to be glucose, sorbose, sucrose and fructose. Fructose was the most abundant one among them. A total of 11 organic acids were identified by capillary gas chromatography and capillary gas chromatography-mass spectrometry. The major components identified were tartaric acid and α-ketoglutaric acid. The total content of vitamin C determined was 386.6mg%, and those of ascorbic, dehydroascorbic, and 2, 3-diketo-L-gulonic acid were 28.8mg%, 154.5mg% and 197.3mg%, respectively. Calcium and phosphorus were the major components, while heavy metals such as cadmium, copper and lead were determined to be a small amount. In the result of organoleptic test on the natural and synthetic extract of Chinese quince fruits, the principal taste components consisted of free amino acids, sugars, organic acids, vitamin C and minerals. Five groups mentioned would have a favorable influence upon the taste of fresh Chinese quince fruits.

• Applied Biological Chemistry
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• v.42 no.2
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• pp.140-146
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• 1999

We have isolated two anticoagulant polysaccharides from an acidic extract of Codium fragile. The purification was conducted using three consecutive chromatographies of DEAE-Toyopearl 650C, Sephadex G-100 (G-75), and Sepharose CL-6B by measuring activated partial thromboplastin time (APTT). The two purified anticoagulant polysaccharides, CF-1-VIa-1 and CF-1-VIIa-1, were found to be nearly homogenous on HPLC using a gel permeation column and appeared to have molecular weights of about 80,000 Da and 40,000 Da, respectively. The polysaccharides consisted mainly of arabinose and galactose in a molar ratio of about 2 : 1, and also comprised 12-13% of sulfates at their constituent sugars. CF-1-VIa-1 and CF-1-VIIa-1 inhibited blood coagulation via both the intrinsic and the extrinsic pathways. The polysaccharides unlike heparin showed an inhibitory activity on thrombin when a pure fibrinogen without antithrombin III was used as a substrate. Structural modifications using sulfation and desulfation affected the anticoagulant activities directly, suggesting that the content of sulfate plays an important role in the blood coagulation cascade. The polysaccharides may inhibit some proteases involved in the blood coagulation cascade, judging from the independence of calcium concentrations in their anticoagulant activity.

• Applied Biological Chemistry
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• v.32 no.2
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• pp.109-115
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• 1989

This study was aimed to find out the influence of long-term fertilization for 21 years on soil enzyme activities in the silty clay loam textured normal paddy soil. Total urease activity (TUA) and the microbial urease activity (MUA) were shown to be changed significantly, but the accumulated urease activity (AUA) was similar within trial plots. Especially the MUA of the plots annually applied N.P.K. fertilizers with compost and N.P.K. fertilizers with silicate fertilizer were the highest among plots. The total L-glutaminase activity (TGA) and the accumulated L-glutaminase activity(AGA) were changed significantly among trial plots, but the microbial L-glutaminase activity (MGA) was not. By the simple correlation analysis, it was shown that the TGA and the AGA correlated highly significant to available phosphorus available $SiO_2$ content and pH. Addition of the toluene to the incubation mixture did not markedly affect the activity of phosphatase, but the difference of phosphatase activity among plots was significant. By the simple correlation analysis, it was shown that the phosphatase activity ; correlated highly significant to pH, available $SiO_2$, available phosphorus and exchangeable calcium in soils.

• Membrane Journal
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• v.18 no.3
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• pp.241-249
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• 2008

This study is to concentrate vanadium in Jeju groundwater using reverse osmosis processes, and to utilize the concentrate for vanadium water. Groundwater samples were taken from Wahyul, Ayum, and Seogwipo groundwater wells with different in vanadium content each other. Their vanadiuln concentrations were 31.8, 44.5, and 53.0 ppb, respectively. The rejection coefficients of every component in groundwater were increased with the increase of TMP At the TMP of $8 kg_f/cm^2$, the rejection coefficients of vanadium, sodium, potassium, aluminium, iron, and barium were $97.4%{\sim}99.0%,\;97.7%{\sim}97.8%,\;98.0%{\sim}98.3%,\;94.8%{\sim}97.5%,\;88.0%{\sim}96.4.0%$, and $97.9{\sim}98.0%$, respectively. And those of magnesium, calcium, chromium, mauganese, and strontium in three groundwater were more than 99.0% at the same TMP. It was possible that vanadium contents of Wahyul, Ayum and Seogwipo groundwater were concentrated into 58.6, 118.9, and 165.1 ppb, respectively, by 6 stages treatment at the recovery ratio of 15%. And these concentrated water (vanadium water) did not exceed the permissible drinking water standards.

• Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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• 2011.10a
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• pp.39-41
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• 2011

The recycling rate of recovered paper in Korea is the highest in the world, 92%, but remanufacturing yield is low due to the extremely poor quality of the paper. The poor quality, in turn, influences to the reject amount in deinking process. To increase the yield of old newspaper recycling process, hydrophobic degree of inorganic pigments of deinking stock must be reduced. To determine the hydrophobicity, Pitch Potential Deposit Tester (PDT) was newly designed and applied with respect to the SB latex property of various quality used in Korea; its hydrophobic degree according to Tg, gel content, charge and particle size of latex and optimum designing condition of SB latex. And below are the conclusions: 1. The reason of excessive reject from old newspaper deinking process for total amount of printed ink is loss of inorganic pigments. When lipase, a biochemical catalyst, was applied with the purpose of preventing inorganic pigments loss about more than 70% of total reject weight and promoting hydration of pulp for deinking, deinking process yield of pre flotation secondary stage increased remarkably without any changes of deinking efficiency. 2. Lipase improved deinking stock by cutting ester linkage on surface of hydrophobic materials to promote its hydration. From this, it reached the conclusion that hydration degree of stock exercises significant effect on flotation deinking process yield. 3. Inorganic alkali promotes hydration of deinking stock. But there have been needs for more fundamental measures other than inorganic alkali of promoting hydration for yield improvement. For this, this study intended to find out reasons of chemical properties change on surface of hydrophobic material by change of pH. 4. Pitch Deposit Test (PDT) was performed for understanding principle of why surface of coating flake from OMG is hydrophobic and why it becomes hydrophilic when pH of stock is alkaline. As a result of this test, it is determined that swelling property by change of pH of latex film, which were used as coating adhesive is the reason for hydrophobic change. 5. Hydrophilicity of coating flake increased with hydrophilic pigments. And as more of SB Latex adhesive was used and higher of calcium hardness of stock became, its hydrophilicity decreased. SB Latex adhesive film is reformed by mechanical friction. For having hydrophilicity under neutral pH, strong bruising action such as kneading is required. 6. Because swelling of adhesive film decreases as Tg of SB latex gets lower and mean diameter gets smaller, it shows hydrophobicity under neutral pH. This lowers hydrophilicity of coating flake, which leads to easy elimination with flotation reject on DIP process. Therefore, for improving future flotation yield, it is necessary to develop to use eco-friendly clean SB latex by raising Tg and increasing mean diameter for recycling, and as a result, to reduce excessive loss of coating flake as a reject from deinking process.

• Journal of Chest Surgery
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• v.44 no.2
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• pp.99-107
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• 2011

Background: Calcification is the most frequent cause of clinical failure of bioprosthetic tissues fabricated from GA-fixed porcine valves or bovine pericardium. A multi-factorial approach using different mechanisms was recently developed to reduce the calcification of bioprosthetic tissues. The purpose of the present study was to evaluate the synchronized synergism of using L-arginine and $NaBH_4$, compared with ethanol and L-lysine, in glutaraldehyde treated porcine pericardium from the standpoint of calcification and tissue elasticity. Materials and Methods: Porcine pericardium was fixed at 0.625% GA (7 days at room temperature after 2 days at $4^{\circ}C$). An interim step of ethanol (80%; 1 day at room temperature) or L-lysine (0.1 M; 2 days at $37^{\circ}C$) or L-arginine (0.1 M; 2 days at $37^{\circ}C$) was followed by completion of the GA fixation. A final step of NaBH4 (0.1 M; 2 days at room temperature) was followed. Their tensile strength, thickness, and thermal stability were measured. Treated pericardia were implanted subcutaneously into three-week-old Sprague-Dawley rats for 8 weeks. Calcium content was assessed by atomic absorption spectroscopy and histology. Results: L-arginine and $NaBH_4$ pretreatment ($1.81{\pm}0.39$ kgf/5 mm p=0.001, $0.30{\pm}0.08$ mm p<0.001) significantly increased tensile strength and thickness compared with the control ($0.53{\pm}0.34$ kgf/5 mm, $0.10{\pm}0.02$ mm). In a thermal stability test, L-arginine and $NaBH_4$ pretreatment ($84.25{\pm}1.12^{\circ}C$, p=0.023) caused a significant difference from the control ($86.25{\pm}0.00^{\circ}C$). L-lysine and $NaBH_4$ pretreatment ($183.8{\pm}42.6$ ug/mg, p=0.804), and L-arginine and $NaBH_4$ pretreatment ($163.3{\pm}27.5$ ug/mg, p=0.621) did not significantly inhibit calcification compared to the control ($175.5{\pm}45.3$ ug/mg), but ethanol and $NaBH_4$ pretreatment did ($38.5{\pm}37.3$ ug/mg, p=0.003). Conclusion: The combined pretreatment using L-arginine and $NaBH_4$ after GA fixation seemed to increase the tensile strength and thickness of porcine pericardium, fixed with GA. Additionally, it seemed to keep thermal stability. However it could not decrease the calcification of porcine pericardium fixed with GA. $NaBH_4$ pretreatment seemed to decrease the calcification of porcine pericardium fixed with GA, but only with ethanol.

• Korean Journal of Medicinal Crop Science
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• v.24 no.5
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• pp.375-385
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• 2016

Background: The study was conducted to elucidate the extraction conditions under which white ginseng has cognition-improving efficacy. Methods and Results: Extracts from white ginseng under different solvent and temperature conditions were analyzed for ginsenoside content and inhibitory effect on N-methyl-D-aspartate (NMDA) receptor and acetylcholinesterase. The total ginsenoside contents and amounts of ginsenoside Rb1 plus ginsenoside Rg1 from the 1st extracts (prepared with EtOH/$H_2O$ as solvent) were higher than those from the 2nd extracts (extracted with $H_2O$ after the 1st EtOH/$H_2O$ extraction). The contents in the 1st and 2nd extracts produced at $80^{\circ}C$ were also higher than those obtained at $50^{\circ}C$. Samples from the 1st extraction at $80^{\circ}C$ indicated higher inhibitory activities on NMDA receptors-whose excessive activation is thought to mediate the calcium-dependent neurotoxicity associated with several neurodegenerative diseases-than those from the 2nd extraction. Among the samples prepared at varying temperatures, the extract prepared at $50^{\circ}C$ showed the highest suppression activity on NMDA receptors. Note, however, that the extracts from the 2nd extraction at $50^{\circ}C$ inhibited acetylcholinesterase-whose inhibition could be a therapeutic strategy for neurodegenerative diseases with cognitive deficits and memory malfunction-more effectively than those from the 1st extraction. Conclusions: To enhance the cognition-improving activity of white ginseng extract, it is suggested that the extracts be utilized after being combined the 1st extracts (made with EtOH/$H_2O$ solvent) and the 2nd extracts (prepared with $H_2O$) at low temperature.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.21 no.4
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• pp.1-10
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• 2018

Use of de-icing salts results in accumulation of high concentrations of ions on roadside soils and tree. The purpose of this study isto determine translocation of seasonal impact of exchangeable cations originating from de-icing salt on roadside surface soil-plant influenced by the intensity of foliar damage (NY = 0-25%, SY = 26-50%, CY = 51-75%) of trees. This paper investigated the concentration of four exchangeable cations ($K^+$, $Ca^{2+}$, $Na^+$, and $Mg^{2+}$) on the roadside surface soil. The tree (Ginko biloba) samples were collected from the Konkuk and Judeok intersections in Chung-ju city. The sequential extraction procedure was applied to 120 soil samples of the soilsurface and 30 tree samples. Four cation exchange ions were determined by ICP-OES. The content of four exchangeable cations present on roadside soil was found to be the lowest in NY but highest in CY from tree pits in the order of NY < SY < CY. Especially, the results were apparent during spring time compared to other seasons. Soil collected from tree pits had the highest concentration of $Ca^{2+}$ possibly due to a higher volume of traffic on those streetsresulting in splashing of more calcium chloride ($CaCl_2$). The analysis of three exchangeable cations ($K^+$, $Mg^{2+}$, and $Na^+$) in the tree leaves revealed higher levels than roadside surface soil when foliar damage ratio increased in the order of NY < SY < CY in summer. In addition, a strong positive linear relationship was observed between the concentration of exchangeable cations in soil and trees. It is hypothesized that the results of this study can be a valuable baseline for managing de-icing salt on roadside soil and trees, in order to mitigate the salt stress that can damage the roadside soil and trees.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.62 no.3
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• pp.241-248
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• 2017

Yield of potato is largely influenced by drought stress. This study was conducted in Gangneung and Cheongju during the spring cropping of potato. Potatoes in the Gangneung area were affected by drought but there was no damage due to drought in Cheongju. During the early-growth stage, the contents of inorganic components like available phosphate and growth characteristics of the potato leaf in Cheongju were significantly higher than those in Gangneung but there was no difference after the flowering stage. It was considered that the potato plants cultivated in Cheongju could vigorously grow than that of Gangneung under drought stress. In addition, the content of calcium (Ca), which is a secondary messenger related to aging, was found to be higher in potato plants grown in Cheongju than in Gangneung and accumulated more quickly in potato plants of Cheongju. Because magnesium (Mg) was also found to be higher in potato plants from Gangneung by a wide margin, this phenomenon was thought be related with drought stress. The amounts of all inorganic components absorbed from soil were higher in Cheongju than in Gangneung, showing a relatively higher plant biomass in Cheongju. Correlations of development indexes related to leaf showed less or no relation in Gangneung. According to yield characteristics of the harvest stage, although yield was greatly reduced under drought stress condition, the rate of commercial yield was not significantly affected under the drought stress condition. Consequently, it was considered that these responses to drought stress could be utilized to stabilize potato production under the stressful conditions associated with abnormal climate.

• Horticultural Science & Technology
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• v.29 no.2
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• pp.144-150
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• 2011

This study was conducted to investigate the nutrient components and physicochemical properties of general ('Superior') and colored potato. Proximate composition, reducing sugar, free sugars, free amino acids, organic acids, minerals were analyzed, and Hunter color values were measured in the study. 'Rose' and 'Blue' (colored potatoes) contained high levels of reducing sugar, and total free sugar content was greatly different according to varieties. Glutamic acid, arginine and ${\gamma}$-aminobutyric acid were detected to be the three major amino acids in colored potato, and the major organic acids of general potato were oxalic acid, tartaric acid, malic acid and citric acid. All potato contained high level of potassium, calcium and magnesium. The Hunter 'L' value was the highest in 'Jaseo'; Hunter 'a' value was high in 'Blue' and 'Jasim'; Hunter 'b' value was the highest in 'Haryoung'. Overall, colored potato had higher amount of nutrients and physicochemical properties than 'Superior'. Therefore, colored potatoes are expected to be highly valuable items for the development and applications of a functional food. In addition, these results will provide fundamental data for improving sitological value, breeding of new cultivar and promoting of roughage usage.