• Journal of Plant Biotechnology
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• v.42 no.3
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• pp.239-244
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• 2015

Calanthe striata f. sieboldii Decne. ex Regel is a terrestrial orchid with beautiful flowers arranged in racemose inflorescences. This species is threatened due to over-collection and loss of suitable habitats. Asymbiotic germination is useful in the conservation efforts to re-establish plants in the wild, and for commercial propagation. In this study, we investigate the effects of sodium hypochlorite (NaOCl), three types of culture media (Phytomax Orchid Maintenance - POM, Seed Germination Maintenance - SGM, and Murashige and Skoog 1962 - MS), and plant growth regulators on embryo swelling, protocorm formation, and embryo diameter of C. striata f. sieboldii. Treatment with 1% NaOCl for 30 min greatly enhanced embryo swelling (28.3%), embryo diameter ($205.8{\mu}m$), and embryo protocorm formation (54.8%) compared to seeds without NaOCl treatment (embryo swelling 8.5%, embryo diameter $14.6{\mu}m$, and protocorm formation 13.4%) on POM medium. Protocorm formation on POM medium supplemented with 1.0 mg/L N6-benzyladenine (BA) (95.6%) was better than the control (54.5%). Additionally, the effects of activated charcoal (AC) and sucrose on seedling growth in in vitro culture were examined. The protocorm converted into healthy plants with well-developed shoot primordia on the POM medium with AC and sucrose. The most suitable conditions for seedling growth after 10 weeks of culture were the POM medium with AC or sucrose. These results show effective asymbiotic seed germination and in vitro seedling growth of C. striata f. sieboldii.

• Korean Journal of Plant Taxonomy
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• v.51 no.3
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• pp.205-249
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• 2021

This study was carried out to survey the flora of Hongdo Island in Sinan-gun, Jeollanam-do, South Korea. Specimens collected from previous Hongdo flora studies were reexamined using a relevant biodiversity database, and field surveys were carried out 22 times from April of 2003 to October of 2020. Based on the specimens collected during both previous studies and this study, the identified vascular plants of Hongdo consisted of 472 taxa comprising 102 families, 296 genera, 425 species, 6 subspecies, and 41 varieties. Among them, 111 taxa are newly recorded in this study, and 6 taxa are described in detail in terms of their morphological characteristics and habitat. Also, 29 taxa were reviewed or re-identified with corresponding taxonomic annotations. In Korea, Hongdo represents the northern distributional limit of the 4 taxa of Goodyera biflora, Damnacanthus major, Calanthe aristulifera, and Hemerocallis hongdoensis. Moreover, Hosta yingeri and Saussurea polylepis are endemic to Hongdo and nearby islands in Korea. Distribution maps of these species were prepared. Protected species designated by the Ministry of Environment were 7 taxa consisting of 2 taxa of level I, specifically Sedirea japonica and Neofinetia falcata, and 5 taxa of level II, which were Cymbidium macrorhizon, Woodwardia japonica, Dendrobium moniliforme, Calanthe aristulifera, and Bulbophyllum inconspicuum. Red list plants as designated by the National Institute of Biological Resources numbered 11 taxa. Naturalized plants numbered 40 taxa.

• Journal of Life Science
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• v.17 no.3 s.83
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• pp.312-317
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• 2007

This study was conducted to research the morphological characteristics and analyze the genetic diversity by using RAPD in Calanthe species native to Korea. Nine samples were selected by flower color and 19 morphological characteristics. In the length and width of leaf, dorsal sepal, the lateral sepal, the petal, the central lip, and the lateral lip, C. discolor was the shortest and narrowest, respectively, but C. sieboldii was the longest and the widest, respectively. The flower stalk length was the shortest in C. discolor, and the longest in C. sieboldii. Three variants were the intermediate between C. discolor and C. sieboldii in the above morphological characteristics, but spur length was the longest in C. discolor, the shortest in C. sieboldii, and intermediate in the variants. The ovary length in C. discolor was shortest and C. sieboldii and variants were similar with each other. The flower color of C. discolor were brownish red, the value of CIE Lab was between 40 and 50. The flower color of C. sieboldii was yellowish, the value of CIE Lab was between 110 and 130. And variants had various colors between 50 to 70 in the value of CIE Lab. By analyzing multiple band patterns of PCR products, 154 bands were selected as polymorphic RAPD markers. The analysis of genetic similarity of Calanther species using RAPD showed that C. discolor and C. sieboldii are more distant from each other than variants, and these results demonstrated that genetic position of variants located between C. discolor and C. sieboldii.

• Korean Journal of Plant Resources
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• v.12 no.1
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• pp.43-49
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• 1999

To develope wild flower collected from Chollabuk-do area resource plant species were investigated from May 1996 to April 1997. This study was focussed to make the basic data for better quality of breeding species from wild flower species and to keep proper gene pool for development with competitive power internationally in future. There was 131 species blooming in spring season(34.7%), 219 species in summer(58.1%), 25 species in autumn(6.6%) and 2 species in winter(0.5%), respectively. Flower color of all the plants was 26 kinds in diverse, among them, white flowers were occupied dominantly of 28.9%, yellow flowers, 20.7%, violet flowers, 16.4% and red purple flowers, 7.9%, respectively. Transplanted wild flower plants at the garden in the study area frequently were Chelidonium majus var. asiaticum, Hemerocallis fulva, Iris nertschinskia, Rhododendron yedoense var. poukhanense, Rhododendron mucronulatum, Pulsatilla koreana, Chrysanthemum zawadskii var. latilobum, Taraxacum platycarpum, Chrysanthemum boreale, Liriope platyphylla and Lycoris radiata. Twenty-six species of wild flowers such as Cymbidium goeringii, H. fulva, L. radiata, C. zawadskii var. latilobum, etc. were collected from their habitat and being selling at the market or road side. Total 343 taxa of wild flowers to prevent carrying out to foreign countries and to improve better quality were selected and recommended in this study, for example, they were 41 taxa of Compositae plant, 25 of Rosaceae, 20 of Liliaceae and 18 of Ranunculaceae in order. Acorus calamus var. angustatus, Cypripedium japonicum, C. macranthum, Calanthe discolor, Dicentra spectabilis, Ilex cornuta, Stewartia koreana, Abeliophyllum distichum, Forsythia saxatilis and Campanula glomerata var. dahurica should be protected as wild flowers because their numbers are decreasing remarkably, belong to rare species, being at a crisis to extinct species and important plants for research materials.