• Animal Bioscience
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• 제35권9호
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• pp.1390-1399
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• 2022

Objective: The objective of this study was to evaluate the effects of dietary supplementation of Schizosaccharomyces pombe (S. pombe) -expressed phytase on growth performance, apparent ileal digestibility, organ indexes, meat quality, toe ash, and footpad lesions score in broiler chicks. Methods: A total of 390 one-day-old broiler chicks were randomly assigned to 5 groups based on the initial body weight (42.15±0.17 g), there were 6 replicate cages per treatment and 13 birds (mixed sex) per cage. The experimental period was 45 days, including 4 periods (starter, days 1 to 10; grower, days 11 to 24; finisher 1, days 25 to 38; finisher 2, days 39 to 45). Dietary treatments were based on a corn-soybean meal-basal diet and supplemented with 500, 750, 1,000, and 1,500 FTU/kg S. pombe-expressed phytase. One phytase unit (FTU) was defined as the amount of enzyme that catalyzes the release of one micromole phosphate from phytate per minute at 37℃ and pH 5.5. Results: The inclusion of increasing levels of phytase in the diet linearly increased the body weight gain during days 1 to 10 (p = 0.001), 25 to 38 (p = 0.016), 39 to 45 (p = 0.018), and 1 to 45 (p = 0.004), feed intake during days 25 to 38 (p = 0.032), feed conversion ratio during days 1 to 10 (p = 0.001), 39 to 45 (p = 0.038), and 1 to 45 (p = 0.012), carcass weight (p = 0.035), toe ash (p<0.001), and apparent ileal phosphorus digestibility (p = 0.049). However, the footpad lesions score (p = 0.040) decreased linearly with the increase in phytase levels in the diet. Conclusion: Dietary supplementation of S. pombe-expressed phytase was beneficial to the growth performance, toe ash, apparent ileal phosphorus digestibility, and footpad lesions of broiler chicks in a dose-dependent manner.

• 현장농수산연구지
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• 제19권1호
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• pp.15-23
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• 2017

전복 방류 효과를 조사한 결과 크기가 큰 종묘(전중, 3.2 g)를 방류한 월항어촌계가 크기가 작은 종묘를 방류한 화태어촌계에 비하여 방류효과가 좋은 것으로 나타났으며, 방류 후 10개월이 경과된 후 월항어촌계의 해삼 생존율은 66%로 매우 높게 나타났다. 이러한 결과는 방류효과가 종묘의 크기에 따라 큰 차이를 보이는 것으로 나타났다. 또한 방류해역에 따른 해삼의 생산효과를 분석한 결과 어류 가두리 양식장이 밀집한 화태어촌계에서 실험종료 시 성장(6.2 g)과 생존율(32.1%)이 가장 좋은 것으로 나타났는데 이것은 오염도는 높지만 지속적으로 동물성먹이가 공급되는 해역의 생산성이 높다는 것을 의미한다. 따라서 자원조성 및 종 보존을 위해서 어류 양식가두리 저면을 활용하는 것도 고려할 방법으로 판단된다.

• 대한통합의학회지
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• 제11권4호
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• pp.135-145
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• 2023

Purpose : The purpose of this study was to discover which breathing technique is more effective in improving respiratory function by applying different breathing techniques-the chest expansion respiratory technique and the abdominal expansion respiratory technique-to adults in the same aerobic exercise situation. Methods : In this study, 30 people were randomly assigned to two groups: chest expansion respiratory technique with aerobic exercise (A group) and abdominal expansion respiratory technique with aerobic exercise (B group). Group A was asked to breathe after wrapping their rib cage with an elastic band, and Group B was asked to breathe after wrapping their abdomen with an elastic band. A total of 3 sets of 30 breaths were performed, 3 times a week for 6 weeks. For statistical processing, an independent t-test was used to verify homogeneity between the two groups, and a repeated measure analysis of variance (ANOVA) was used to compare differences according to the period for each group. Results : The changes in FVC and FEV₁ for each group following 6 weeks of intervention are as follows. In comparison according to the intervention period, there was a significant increase in all groups depending on the timing of measurement (p<.05), and as a result of the inter-subject effect test, there was no significant difference between groups (p>.05). In addition, there was no interaction effect between period and group (p>.05). Conclusion : The conclusion of this study is that FVC and FEV₁ values increased according to the intervention period in both groups; therefore, it can be suggested that flexible breathing techniques can be applied depending on the patient's physical situation when applying a breathing program to improve respiratory function.

• 대한통합의학회지
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• 제12권2호
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• pp.55-63
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• 2024

Purpose : The purpose of this study is to determine the effects of scapula and pelvis symmetrical reciprocal pattern exercise of PNF concept on respiratory function and quality of life in elderly subjects. Methods : Nineteen elderly subjects with healthy were recruited. Subjects performed scapula and pelvis symmetrical reciprocal pattern exercise of PNF concept. Exercise was appied 30 minutes three times per week for four weeks. The respiratory function and quality of life (QOL) test three times (before, two weeks, and four weeks). Respiratory function includes forced vital capacity (FVC), forced expiratory volume at one second (FEV₁), Peak Expiratory Flow (PEF) and chest cage expansion test (CCET). The QOL test was measured Korean WHOQOL-BRIEF. The analysis method was analyzed through the one-way ANOVA repeated methods, and the statistical significance is α=.05. It was analyzed through the post test Bonferroni test. Results : After the 4 week scapula and pelvis symmetrical reciprocal pattern exercise of PNF concept showed statistically significant differences in the respiratory function (FVC, FEV₁, PEF, and CCET), and QOL (p<.05). As a result of the post-hoc test, FVC showed a significant increase in the mid test and post test compared to the pre test (p<.05), FEV₁ showed a significant increase in the post test compared to the pre test (p<.05). PEF showed a significant increase in all pre-test, mid-test, and post-test sections (p<.05), CCET showed a significant increase in all pre-test, mid-test, and post-test sections (p<.05). QOL showed a significant increase in all pre-test, mid-test, and post-test sections (p<.05). Conclusion : In this study, the scapula and pelvis symmetrical reciprocal pattern exercise of PNF concept improved respiratory function and QOL. The findings suggest that this intervention could be beneficial in improving respiratory function and QOL in the elderly.

• FOCUS: LIFE SCIENCE
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• 제1호
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• pp.2.1-2.6
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• 2024

This article provides detailed instructions for the correct installation, maintenance, and troubleshooting of capillary gas chromatography (GC) columns. It emphasizes the importance of proper installation to ensure optimal performance and longevity of the column. The document covers various aspects such as column trimming, installation, conditioning, testing, storage, and ferrule selection. The installation process involves ensuring that the heated zones of the GC are cool before placing the column cage in the column oven. It is essential to avoid sharp bends or stress on the capillary column during installation and to connect the front end of the column into the GC inlet at the recommended insertion distance. The document also provides guidance on trimming the column, including the use of a ceramic wafer or capillary column cutter to achieve a clean, burr-free cut. For previously used columns, it recommends removing any capillary caps, positioning the nut and ferrule, and trimming 1-2 cm from the column. After installation, the column should be purged with carrier gas to remove any oxygen and avoid oxidizing the column. Conditioning the column involves ramping to the upper isothermal temperature limit and maintaining this temperature for a specified duration. It is crucial to maintain carrier gas flow during conditioning and not exceed the upper temperature limit of the column to avoid phase damage. The document also discusses testing column performance using a suitable method and performing a test injection to assess performance. It provides recommendations for column storage, including flame-sealing the capillary ends or using retention gaps for long-term storage. Additionally, it emphasizes the importance of routine maintenance and replacement of GC consumables to extend the column's lifetime. Ferrule selection is another important aspect covered in the article, with a variety of ferrule materials available for different applications. The characteristics of common ferrule options are presented in a table, including temperature limits, reusability, and suitability for specific detector types.

• 생물정신의학
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• 제6권2호
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• pp.240-245
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• 1999

Object : This study was designed to evaluate the effects of olanzapine on the schedule-induced polydipsia(SIP) which is one of animal model of obsessive-compulsive disorder in rats. We administered olanzapine as a serotonin and dopamine blocking agent, fluoxetine as a selective serotonin reuptake inhibitor, and haloperidol for the dopamine antagonist to rats which showed schedule-induced polydipsic behavior. Methods : Spraque-Dawley rats weighing 200-250gm were individually housed and maintained and allowed free access to water. The rats were placed on a restricted diet. To induce polydipsia, rats were placed in the cage where a pellet dispenser automatically dispensed 90mg pellets on a fixed-time 60 seconds(FT-60s) feeding schedule over 150 minute test session per day. Water was available at all times in the cage. After 4 weeks of daily exposure to the FT 60s feeding schedule, experimental rats met a predetermined criterion for polydipsic behavior(greater than 3 times of water per session on average). 5 groups of rats were administered olanzapine(3mg/kg, i.p), olanzapine(10mg/kg, i.p), fluoxetine(5mg/kg, i.p.), haloperidol(0.1mg/kg, i.p.), and vehicle(1cc/kg, i.p.) for 3 weeks. The rats were tested once a week to access schedule induced polydipsic behavior. Water bottles were weighed before and after the 150-minute test session. The chronic effects of administration of experimental drugs on schedule induced polydipsic behavior were analyzed with ANOVA and Scheffe test as a posthoc comparison. In order to measure water consumption in non-polydipsic food-deprived rats, a separate group of rats(N=8) were individually housed and given a single bolus(14.5gm) of food per day which maintained them at their average body weight. Results and Conclusion : The results were as follows ; 1) After 4 weeks of scheduled feeding procedure, the experimental group showed significant differences than the bolus control in the amount of water consumption as compared with their average water intakes for 4 weeks. At the same periods, there were no differences between the experimental group and the bolus control in the body weight. 2) The fluoxetine group showed significant decrease in the amount of water intake over the 3 weeks of drug treatment as compared with their average amount of polydipsic water intakes. The olanzapine 3mg group showed significant decrease in the amount of water intake at 3rd weeks of drug treatment as compared with their average amount of polydipsic water intakes. The olanzapine 10mg group showed significant decrease in the amount of water intake at 2nd and 3rd weeks of drug treatment as compared with their average amount of polydipsic water intakes. However, the haloperidol group and the vehicle control group showed no changes of amounts of water intake for 3 weeks of treatment as compared with their average amount of polydipsic water intakes. 3) The fluoxetine group showed significantly lower amounts of water intake than the haloperidol group at 2nd weeks of drug treatment. And also the fluoxetine group showed significantly lower amounts of water intake than the haloperidol group and the vehicle control at 3rd weeks of drug treatment. The olanzapine 3mg group and the olanzapine 10mg group showed significantly lower amounts of water intake than the haloperidol group and the vehicle control at 3rd weeks of drug treatment. Above findings suggest that the fixed time feeding procedure for schedule-induced polydipsia as an animal model of obsessive compulsive disorder was effective to the evaluation of pharmacological challenge study. The authors assume that the serotonin hypothesis and the serotonin-dopamine interaction hypothesis are preferred to the dopamine hypothesis in the biological etiology of obsessive-compulsive disorder.

• The Korean Journal of Physiology
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• 제2권1호
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• pp.79-88
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• 1968

유두체가 일반활동 및 스트레스 기전에 어떠한 영향을 끼치는 지를 알기 위하여 유두체가 제거된 흰쥐들(유두체군), 유두체군과 마찬가지 수술 조작을 가하였으나 유두체는 제거하지 않은 흰쥐들(수술 대조군) 및 정상 흰쥐들(정상 대조군)을 마련하여 두 가지 실험을 실시하였다. 유두체 제거에 있어서는 뇌 정위 고정법에 의하여 동물을 좌우 유두체에 각각 전극(+)을 심고 이와 항문에 넣은 전극(-) 사이에 0.3 ma의 직류 전류를 7초 동안 흘려 유두체를 파괴하였다. 첫째 실험에서는 광선차단 회수 측정 방법에 의하여 유두체군(9마리)의 일반활동을 48시간 동안 측정하여 수술 대조군(13마리)의 그것과 비교하였으며, 둘째 실험에서는 $-10^{\circ}C$의 추위에 한 시간 동안 폭로하는 것을 스트레스로 삼고 추위에 폭로되기 전, 폭로가 끝난 직후, 2시간 후 및 4시간 후에 유두체군(52마리), 수술 대조군(45마리), 및 정상 대조군(37마리)의 부신 아스코르빈산 함유량을 측정하여 스트레스에 대한 반응 정도를 비교하였다. 실험결과는 다음과 같다. 1. 48시간 동안의 일반활동을 총괄한 성적을 비교할 경우에는 유두체군과 수술 대조군 사이에 유의한 차이가 없으나 유두체군의 일반활동은 수술 대조군에 비하여 다소 증가하는 경향이 있었으며, 저녁 때와 실험이 시작된 직후의 몇 시간 동안은 유두체군의 값이 수술 대조군의 값 보다 유의하게 많았다. 2. 스트레스를 받고 난 직후의 유두체군의 부신 아스코르빈산 함유량은 스트레스를 받기 전에 비하여 유의하게 감소되어 정상 대조군 및 수술 대조군과 마찬가지로 스트레스에 대한 반응이 현저하였다. 그러나 유두체군과 수술 대조군은 정상 대조군 보다 스트레스로 부터의 회복과정이 각각 유의하게 또는 다소 촉진되어 있었다. 위의 결과로 미루어 유두체는 정상시 일반활동에 미약하나마 억제성 영향을 끼치는 경향이 있으며 스트레스 기전에는 주도적인 역할을 하지 않는 것으로 추측되나 이들 문제를 결정짓기에 앞서 많은 연구가 요망된다.

• 한국해양학회지:바다
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• 제9권2호
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• pp.64-72
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• 2004

유기물이 많이 유입되는 해상어류가두리 양식장 퇴적물에서 전극 크기가 25$mu extrm{m}$인 미세전극을 이용하여 공극수의 산소, 황화수소, pH의 미세연직 농도를 측정하였다. 산소와 황화수소의 연직분포에서 얻어진 미세구간에 1차 확산ㆍ반응모델을 적용하여 각 구간에서의 산소 소모율, 황화수소 산화율, 황산염의 환원율을 추정하였다. 산소투과깊이는 0.75 mm였으며, 미세구간은 상부와 하부층 2개로 나누어졌다. 산소소모는 황화수소의 산화 영향으로 상부층에 비해 하부층에서 높았고 총산소소모플럭스는 0.092 $\mu$mol $O_2$$cm^{-2}$ $hr^{-1}$였다. 산화층에서 황화수소 산화는 0.7 mm 두께에서 0.030$\mu$mo1 H$_2$S $cm^{-2}$ $hr^{-1}$의 결과를 나타냈으며, 이 곳에서 황화수소의 turnover time은 약 2분으로 화학적 산화와 생물학적 산화가 동시에 일어나고 있었다. 황화수소와 산소의 소모율 비는 0.84로 황화수소 산화에 산소 이외의 다른 전자수용체가 사용되거나 산소-황화수소 경계면 주변에서 황 침전의 가능성을 시사하고 있었다. 추정된 총 황산염 환원 플럭스는 0.029$\mu$mol $cm^{-2}$ $hr^{-1}$로서 총산소소모플럭스의 60% 이상을 차지하고 있어 무산소 환경에서 유기물 분해가 산화환경에서보다 큰 역할을 하는 것으로 나타났다.

• 한국산림과학회지
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• 제102권2호
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• pp.170-175
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• 2013

소나무재선충병 방제법 중 임업적 방제법을 개발하기 위한 일환으로 숲가꾸기 시 발생하는 어린 소나무림(수고 1~2 m)과 장령 소나무림(수고 9~10 m)의 건전한 벌근(伐根)이 소나무 재선충의 매개충인 솔수염하늘소의 서식에 미치는 영향을 구명하기 위하여 야외 그물망 케이지(1 m ${\times}$ 1 m ${\times}$ 1 m)내에 어린 소나무림과 장령 소나무림별로 4가지 종류의 처리를 하였다(2010년 2월, 2011년 2월, 4월, 6월). 2010년 6월 하순에 각 케이지 마다 4~5쌍의 솔수염하늘소 성충을 방사한 후 2011년 8월말까지 후식(maturation feeding)시켰다. 2011년 10월말에 각 시료목에 출연하는 유충의 침입공과 서식여부를 조사하였다. 그 결과 솔수염하늘소의 우화 당년도인 2011년 2월, 4월, 6월에 숲가꾸기한 어린 소나무림과 장령 소나무림의 건전한 벌근에서는 솔수염하늘소 유충이 서식하였으나 솔수염하늘소 방사 16개월전인 2010년 2월에 숲가꾸기한 건전한 벌근에서는 솔수염하늘소의 유충이 출현하지 않았다. 어린 소나무의 벌근에 서식하는 유충은 장령 소나무의 벌근에 서식하는 유충에 비하여 몸통 크기가 작게 나타났으며, 장령 소나무림벌근의 경우 수피의 요(凹)형 부위(할목 부위)에서 유충이 서식하는 경향이었으며, 출현한 유충 모두가 수피부나 수피부와 목질부 사이에 서식하고 있었으나 목질부에서는 출연하지 않았다. 따라서 숲가꾸기한 건전목의 벌근처리에 대한 방제 지침을 마련을 위하여 건전목의 벌근 높이에 따른 매개충의 산란 및 유충 서식여부에 대한 조사가 요구된다.

• Bulletin of the Korean Chemical Society
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• 제30권6호
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• pp.1285-1292
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• 2009

Large single crystals of zeolite, |$Na_{75}$|[$Si_{117}Al_{75}O_{384}$]-FAU (Na-Y, Si/Al = 1.56), were synthesized from gels with composition of 3.58Si$O_2$ : 2.08NaAl$O_2$ : 7.59NaOH : 455$H_2$O : 5.06TEA : 2.23TCl. One of these, a colorless single-crystal was ion exchanged by allowing aqueous 0.02 M CsOH to flow past the crystal at 293 K for 3 days, followed by dehydration at 673 K and 1 ${\times}\;10^{-6}$ Torr for 2 days. The crystal structure of fully dehydrated partially $Cs^+$-exchanged zeolite Y, |$Cs_{45}Na_{30}$|[$Si_{117}Al_{75}O_{384}$]-FAU per unit cell (a = 24.9080(10) $\AA$) was determined by single-crystal X-ray diffraction technique in the cubic space group Fd $\overline{3}$ m at 294(1) K. The structure was refined using all intensities to the final error indices (using only the 877 reflections with $F_o\;>\;4{\sigma}(F_o))\;R_1$ = 0.0966 (Based on F) and $R_2\;=\;0.2641\;(Based\;on\;F^2$). About forty-five $Cs^+$ ions per unit cell are found at six different crystallographic sites. The 2 $Cs^+$ ions occupied at site I, at the centers of double 6-ring (D6Rs, Cs-O = 2.774(10) $\AA$ and O-Cs-O = 88.9(3) and 91.1(3)$^o$). Two $Cs^+$ ions are found at site I’ in the sodalite cavity; the $Cs^+$ ions were recessed 2.05 $\AA$ into the sodalite cavity from their 3-oxygen plane (Cs-O = 3.05(3) $\AA$ and O-Cs-O = 77.4(13)$^o$). Site-II’ positions (opposite single 6-rings in the sodalite cage) are occupied by 7 $Cs^+$ ions, each of which extends 2.04 $\AA$ into the sodalite cage from its 3-oxygen plane (Cs-O = 3.067(11) $\AA$ and O-Cs-O = 80.1(3)$^o$). The 26 $Cs^+$ ions are nearly three-quarters filled at site II in the supercage, being recessed 2.34 $\AA$ into the supercage (Cs-O = 3.273(8) $\AA$ and O-Cs-O = 74.3(3)$^o$). The 4 $Cs^+$ ions are found at site III deep in the supercage (Cs-O = 3.321(19) and 3.08(3) $\AA$), and 4 $Cs^+$ ions at another site III’ (Cs-O = 2.87(4) and 3.38(4) $\AA$). About 30 $Na^+$ ions per unit cell are found at one crystallographic site; The $Na^+$ ions are located at site I’ in the sodalite cavity opposite double 6-rings (Na-O = 2.578(11) $\AA$ and O-Na-O = 97.8(4)$^o$).