• Journal of the Korean Institute of Rural Architecture
• /
• v.16 no.2
• /
• pp.1-8
• /
• 2014

In the past, Korea was classified as a region not affected by earthquakes. However, recent increase of earthquakes has forced to strengthen standards of earthquake resistant designs of structures to minimize seismic damage. In addition, it was thought that masonry infill walls in buildings are only acting as partitions, so these walls are not considered in analyzing building structures. But it was found that when seismic loads are applied to a structure with masonry infill walls, the walls affect the structure. Accordingly, this study conducted nonlinear static analyses for a structure constructed before applying earthquake resistant designs in two cases: when considering masonry walls and when not. The result showed that the seismic performance of the structure is insufficient. Thus, the structural resistance of the structure was also studied in two cases: when reinforcing with steel plate braces and when using carbon fiber braces. In the two cases reinforcing two different stiffeners, it was appeared that the behaviors of the structure were similar, though the cross-section area of a carbon fiber brace used to reinforcing the structure is only 12.6% of a steel plate brace, and its weight is only 2.8%. Thus, the reinforcing effect of the thin, light-weighted carbon fiber brace is much larger than that of the steel plate brace, when considering usability and constructability of both materials.

• Journal of Microbiology and Biotechnology
• /
• v.5 no.4
• /
• pp.188-193
• /
• 1995

A Brevibacterium flavum gene coding for glucose permease of the phosphoenolpyruvate-dependent phosphotransferase system (PTS) was cloned by complementing the Escherichia coli ZSCl13 mutations affecting a ptsG gene with the B. flavum genomic library. From the E. coli clone grown as red colony on a MacConkey plate supplemented with glucose as an additional carbon source, a recombinant plasmid was isolated and named pBFT93. The plasmid pBFT93 was identified as carrying a 3.6-kb fragment of B. flavum chromosomal DNA which enables the E. coli transformant to use glucose or man nose as a sole carbon source in an M9 minimal medium. The non-metabolizable sugar analogues, 2-deoxy-D-glucose (2-DG) and methyl-$\alpha$-D-glucopyranoside (MeGlc) affected the growth of ZSCl13 cells carrying the plasmid pBFT93 on minimal medium supplemented with non-PTS carbohydrate, glycerol, as a sole cabon source, while the analogues did not repress the growth of ZSCl13 cells without pBFT93. It was also found that both $2-deoxy-D-[U-^{14}C]glucose{\;}and{\;}methyl-{\alpha}-D-[U-^{14}C]glucopyranoside$ could be effectively transported into ZSCl13 cells transformed with plasmid pBFT93. Several in vivo complementation studies suggested that the B. flavum DNA in pBFT93 encodes a glucose permease specific for glucose and mannose.