• 미생물학회지
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• 제9권4호
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• pp.145-148
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• 1971

Mitochondria were isolated from Chlorella cells effects of cation on solute permeability of mitochondrial membrane were investigated using P$^{32}$ as a tracer. It was strikingly increased uptake of phosphate for NaCl, KCl, while evidently decreased phosphate uptake of mitochondrial membrane for $MgCl_2$, $CaCl_2$. This consider that uptake of monovalent cation were increased, but uptake of divalentcation were decreased for permeability of mitochondrial membrane as if the permeability of protoplasmic membrane.

• 패션비즈니스
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• 제13권1호
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• pp.115-124
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• 2009

Chitosan and CA/BTCA were employed in order to induce crosslinking in the fiber for the improvement of wrinkle recovery of the cotton fabrics and the endowment of anti-microbial functions to the fabric. The treated fabrics were dyed by using reactive dyestuff and their dyeing behaviors were investigated. As a result, the fabric treated with chitosan only exhibited more dye-uptake amount than the untreated fabric, and the treated and untreated fabrics together showed excellent light-fastness and wash-fastness. In the cases that CA or BTCA was added to the chitosan, the CA-treated showed better dye-uptake and dyeing properties as light-fastness than the BTCA-treated. In conclusion, it is possible to maintain the dye-uptake level at reduced treatment cost when the CA is employed as a substitute cross-linking agent for BTCA.

• The Korean Journal of Ecology
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• 제15권2호
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• pp.157-172
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• 1992

Studies were conducted to evaluate the differential al tolerance of suwon 205 and olmil wheats grown is nutrient solution and determine the impact of NH4+-N or Ca2+ nutrition of the response of the two wheat vareties to al. In various concentrations of al, olmil induced lower ph levels than suwon 205 in nutrient solutions and the reduction of root length and dry weight was greater in olmil than in suwon 205. The uptake of al was greater in root than in shoot of the two wheat varieties, but more increased in root of olimil. also the uptake of ca, mg, k and p was inhibited by al especialy decrease of ca and p uptake in roots of olmil was more pronounced than in those of suwon 205. In nutrient solutions that contained NH4+-N plus 9ppm al, the ability of both varienties to raise the ph was reduced as the level of NH4+-N in nutrient solutions was increased, and al-sensitive olimil induced lower ph than did al-tolerant suwon 205. al toxicity was intensified by increasing the concentration of NH4+-N in nurient and toxic effect was greater in olmil. al toxicity in the two wheat varieties was steadily increased as the ca level of nutrient solution was reduced, sepecialy this effect was stronger in al-sensitive olmil than in al-tolerant suwon 205.

• The Korean Journal of Physiology and Pharmacology
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• 제3권2호
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• pp.175-182
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• 1999

In the present study, the underlying mechanisms for diabetic functional derangement and insulin effect on diabetic cardiomyopathy were investigated with respect to sarcoplasmic reticulum (SR) $Ca^{2+}-ATPase$ and phospholamban at the transcriptional and translational levels. The maximal $Ca^{2+}$ uptake and the affinity of $Ca^{2+}-ATPase$ for $Ca^{2+}$ were decreased in streptozotocin-induced diabetic rat cardiac SR, however, even minimal amount of insulin could reverse both parameters. Levels of both mRNA and protein of phospholamban were significantly increased in diabetic rat hearts, whereas the mRNA and protein levels of SR $Ca^{2+}-ATPase$ were significantly decreased. In case of phospholamban, insulin treatment reverses these parameters to normal levels. Minimal amount of insulin could reverse the protein levels; however, it could not reverse the mRNA level of SR $Ca^{2+}-ATPase$ at all. Thus, the decreased SR $Ca^{2+}$ uptake appear to be largely attributed to the decreased SR $Ca^{2+}-ATPase$ level, which is further impaired due to the inhibition by the increased level of phospholamban. These results indicate that insulin is involved in the control of intracellular $Ca^{2+}$ in the cardiomyocyte through multiple target proteins via multiple mechanisms for the decrease in the mRNA for both SR $Ca^{2+}-ATPase$ and phospholamban which are unknown and needs further study.

• 한국토양비료학회지
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• 제30권2호
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• pp.178-183
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• 1997

본 실험는 토마토 촉성재배시(促成栽培時) 시설내(施設內) 고습환경(高濕環境)에서 높은 배꼽썩음과(果) 발생(發生)과 근권부의 Ca흡수와의 관련성을 구명하기 위해 수행(遂行)하였다. 1) 가습처리(加濕處理)의 시설내(施設內) 기온(氣溫)은 제습(除濕) 하우스보다 $3.1^{\circ}C$ 낮았고, 상대습도(相對濕度)는 12시경(時傾)에 약(約) 70%로 제습(除濕) 하우스보다 20% 높았다. 2) 근건물중당(根乾物重當) 도관(導管)을 통한 수분유출속도(水分流出速度)는 배꼽썩음과가 23%발생된 가습처리(加濕處理)가 배꼽썩음과(果)가 발생(發生)되지 않은 제습처리(除濕處理)보다 $0.24g{\cdot}g^{-1}{\cdot}h^{-1}$ 빨랐고, Ca, Mg, K, Mn, Zn의 도관유출속도(導管流出速度)도 빨랐다. 3) 도관(導管)의 유출액(流出液)과 수경액(水耕液)의 무기성분농도(無機成分濃度)는 유출액(流出液)의 Ca, Mg, K가 수경액(水耕液)보다 2~4배높고, Mn, Zn은 양액(養液)이 다소높았다. 이상의 결과(結果)를 종합(綜合)하면 토마토 촉성재배시(促成栽培時) 고습(高濕)에의해 배꼽썩음과(果) 발생(發生)이 많은 것은 근권부(根圈部)의 Ca흡수 저해(沮害)보다는 흡수된 Ca의 식물체내(植物體內) 분배(分配)의 장해(障害)라고 생각된다.

• 대한약리학회지
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• 제31권3호
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• pp.291-297
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• 1995

본 연구의 목적은 protein kinase C의 활성물질인 phorbol ester의 수축에 대한 cGMP 및 cAMP의 조절기전을 명확히 하기 위하여 흰쥐의 대동맥을 재료로 실험을 수행하였다. Sodium nitroprusside는 guanylyl cyclase를 활성화시켜 cGMP를, forskolin은 adenylyl cyclase를 활성화시켜서 cAMP를 증가시키는 것으로 보고되어 있으므로 위의 두 약물을 선택하였다. Phorbol ester는 시간경과와 함께 지속적인 수축을 발생하였으며 30분경 안정상태에 도달하였다. 동시에 20-kDa myosin light chain (MLC)의 인산화도 증가하였으며 30분경 최대치를 나타내었다. Sodium nitroprusside와 forskolin은 phorbol ester에 의한 수축을 농도의존적으로 억제하였으나 sodium nitroprusside가 forskolin보다 더욱 민감하게 억제하였다. Phorbol ester는 $^{45}Ca^{2+}$의 유입을 증가시켰고 sodium nitroprusside와 forskolin은 이 증가된 $^{45}Ca^{2+}$을 유의하게 억제하였다. Phorbol ester에 의하여 증가된 MLC의 인산화는 sodium nitroprusside 및 forskolin 각각의 최대농도로 억제되었다. 이상과 같은 결과로 볼때 아마도 cGMP와 cAMP는 phorbol ester에 의한 수축을 $^{45}Ca^{2+}$ 유입억제에 이은 MLC 인산화 억제에 의하여 이완작용을 나타내는 것으로 추측되며 cGMP가 cAMP보다 protein kinase C 매개의 수축조절에 더 중요하게 작용하리라 추측된다.

• 한국토양비료학회지
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• 제16권3호
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• pp.235-241
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• 1983

토성(土性)이 상이(相異)한 3개토양(個土壤)에서 질소시용량(窒素施用量)을 달리하여 벼의 생육(生育)이 각각(各各) 다른 조건에서 가리시용(加里施用)이 주요(主要) 양(陽)이온의 흡수(吸收)와 용탈(溶脫)에 미치는 영향(影響)을 찾기 위한 pot시험(試驗)에서 다음과 같은 결과(結果)를 얻었다. 1. 가리시용(加里施用)으로 가리(加里)의 흡수(吸收)는 증가(增加)되고 석회(石灰)와 고토(苦土)의 흡수(吸收)는 감소(減少)되었으며 정조수량(正租收量)은 증가(增加)되었다. 2. 가리시용(加里施用)은 $Ca^{+{+}}$, $Mg^{+{+}}$, $K^{+}$, $NH_4$와 같은 양(陽)이온의 용탈(溶脫)을 증가(增加)시켰다. 3. 출수기(出穗期) 엽중(葉中)의 적절(適切)한 양(陽)이온비(比)는 N중간수준구(中間水準區)에서는 K/Ca 1.59, K/Mg 4.26 $K_2O/N$ 3.62이며 N최고수준구(最高水準區)는 K/Ca 1.65 K/Mg 4.32 $K_2O/N$ 3.94 이었다. 4. 시험전(試驗前) 토양(土壤)의 양이온비(比)($K/\sqrt{Ca+Mg}$)는 가리시용(加理施用)한 후 벼의 생육기간중 식물체(植物體)의 양이온비 ($K/\sqrt{Ca+Mg}$)와 용탈용액의 양이온비($K/\sqrt{Ca+Mg}$) 및 수확 후 토양중의 양이온비(比)($K/\sqrt{Ca+Mg}$)와 거의 같은 값을 내었다.

• 약학회지
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• 제35권3호
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• pp.174-181
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• 1991

These experiments were conducted to investigate the effects of glycyrrhizin(GL) and glycyrrhetinic acid(GA) on histamine synthesis, lymphocyte blastogenesis in C57BL/6J mice splenocytes, IL-1 production, $Ca^{2+}$ uptake by macrophage-like P388D$_{1}$ cells and plaque forming cell assay against SRBC. Histamine contents, lymphocyte blastogenesis, IL-1 activity, $Ca^{2+}$ uptake and plaque forming cell were determined by enzyme isotope method, [sup 3/H]-thymidine incorporation, C3H/HeJ mouse thymocytes proliferation, the addition of 5 $\mu$Ci/ml $^{45}$Ca$^{2+}$ to P388D$_{1}$, cell suspension and assay to sheep red blood cell, respectively. Cytotoxicity, which was expressed as 50% mortality, was occurred by the addition of GL(10$^{-3}$M) and GA(10$^{-4}$M). Histamine production in mouse spleen cell culture was significantly increased by the addition of 0.25 $\mu\textrm{g}$/ml of Con A, after 48 hour incubation. Con A dependent T-lymphocyte proliferation was also enhanced by the addition of 0.25 .mu.g/ml of Con A. The effects of GL on histamine contents and T-lymphocyte proliferation were significantly decreased at high dose (10$^{-5}$M), while IL-1 activity was remarkably suppressed by 10$^{-8}$~10$^{-4}$M of GL. $Ca^{2+}$ uptake was not changed, but antibody production was increased by GL(10 mg/kg). GA inhibited histamine contents at 10$^{-9}$~10$^{-7}$ and depressed Con A (0.25 $\mu\textrm{g}$/ml) dependent T-lymphocyte proliferation at 10$^{-7}$~10$^{-5}$M of GA, but increased suboptimal dose (Con A 0.1 $\mu\textrm{g}$/ml) at 10$^{-9}$~10$^{-7}$M of GA. IL-1 activity was suppressed by 10$^{-8}$~10$^{-4}$M of GA and $Ca^{2+}$ uptake was enhanced by 10$^{-9}$~10$^{-6}$ of GA, but antibody production was not changed by GA. From the above results, it is suggested that GL and GA have immuno-regulatory action. GL decreased cell-mediated immune response, and increased humoral immune response at high dose. On the other hand, low dose of GA enhanced cell-mediated immune response, while high doses of GA decreased humoral immune reaction.

• 한국동물학회지
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• 제13권3호
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• pp.85-93
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• 1970

미토콘드리아의 Ca 결합은 물질대사에 의존하지 않는 初期 結合과 에너지 소비를 수반하는 膜透過의 二段階로 일어 난다는 설이 있다. 본 실험에서는 이를 확인키 위하여 흰쥐의 肝에 서 抽出한 미토콘드리아를 $^45 CaCl_2$를 함유한 sucrose-tris chloride 용액에서 incubate 시키면 서 Ca 흡수, 산소소비 및 ATPase 活性을 측정하였다. 미토콘드리아의 Ca 결합량은 온도의 영향을 거의 받지 않으며, succinate 나 ATP의 존재에 의해서도 증가하지 않는다. 반면 산소소비량은 succinate의 존재에 의하여 현저하게 증가되며 또 온도의 상승에 따라 증가된다. ATPase 活性도 온도의 상승에 따라 증가한다. 산소소비량과 Ca의 결합량이 비례하지 않는 것으로 보아 미토콘드리아의 Ca 초기 결합은 물질 대사에 의존하지 않는 것으로 판단된다. 미토콘드리아의 ATPase 活性은 DNP 의 존재에 의하여 증가된다.

• Animal cells and systems
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• 제14권4호
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• pp.245-252
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• 2010

Iron uptake in mitochondria and fractionated mitochondria compartments was studied to understand iron transport in heart mitochondria. The inner membrane is most active in iron uptake. Mitochondrial uptake was dependent on iron concentration and the amount of mitochondria. Iron transport was inversely proportional to pH in the range of 6.0 to 8.0. Iron transport reached a maximum after 30 min of incubation at $37^{\circ}C$. Iron uptake was inhibited by 1 mM ATP and stimulated by 1 mM ADP. The oxidative phosphorylation inhibitor oligomycin inhibited iron uptake, but rotenone and antimycin A did not. The divalent ions $Mg^{2+}$, $Cu^{2+}$, $Mn^{2+}$, and $Zn^{2+}$ suppressed iron uptake at $10\;{\mu}M$ and stimulated it at 1 mM. The divalent ion $Ca^{2+}$ stimulated iron uptake at $10\;{\mu}M$ and suppressed it at 1 mM, competing with iron. The uptake of calcium was stimulated by 10 to $1000\;{\mu}M$ ATP, while iron uptake was stimulated reciprocally by 10 to $1000\;{\mu}M$ ADP, suggesting that these ions have movements similar to those of ATP and ADP.