• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.6
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• pp.995-999
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• 2004

Effects of liquefied calcium supplement on bone mineral density (BMD) and bone health index (osteocalcin, deoxypyridinoline) were investigated in 12 middle aged women. Middle aged women were arranged into 3 groups such as control, Ca500 and Ca750 by liquefied calcium supplement level. BMD was measured at the spine (vertebrae L2-L4). After 6 month, BMD of control group dropped but that of Ca750 were increased from 0.850 to 0.865. The increase in serum calcium and osteocalcin level, and the decrease in urine deoxypyridinoline level were observed in by calcium supplement groups. The bone health index of serum was changed as much as level of calcium supplement. The serum osteocalcin level of Ca750 significantly increased to 7.93 ng/mL after 6 months of calcium supplement. Although we didn't get any significant difference in BMD, we found that the liquefied calcium had no side effect and led effective change in bone health index. Hereafter, we suppose that the liquefied calcium will be available to develop healthy products for preventing osteoporosis.

• Journal of Animal Science and Technology
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• v.45 no.3
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• pp.455-462
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• 2003

A study was conducted to determine the effects of feeding a diet containing no Ca supplement (limestone) during the late finishing period on growth, marbling and serum 1,25-dihydroxy vitamin $D_3$ level in Korean native cattle. Twenty-four steers (20${\sim}$24 mo of age) were divided into two groups of 12 each: one group assigned to a control diet (concentrates containing 2.5% limestone) and the other to a diet containing no calcium supplement. They were allowed to have free access to diets (concentrates and orchard grass hay) and water during the entire feeding period (223 d). Serum $Ca^{2+}$, Ca and P concentrations were not influenced by diets, but serum 1,25-dihydroxy vitamin $D_3$ concentrations determined 2 or 6 mo after the beginning of feeding the experimental diets were higher (P<0.01) in steers fed the diet without Ca supplement than in those fed the control diet (78.3 vs 51.7 and 80.3 vs 51.1 pg/mL, respectively). Steers fed the diet without Ca supplement tended to have a higher intake of concentrates, but a lower intake of hay, compared to those fed the control diet. Average daily gain was higher (P<0.05) in steers fed the diet without Ca supplement than in those fed the control diet. Feeding the diet without Ca supplement remarkably (P<0.01) increased the marbling score (5.1 vs 2.2) and the muscle (M. longissimus dorsi) fat content (10.2 vs 6.7%) with a concomitant decrease in moisture content (67.6 vs 70.4%), compared to feeding the control diet. Ribeye area was increased (77.2 vs 82.8 $cm^2$) with the diet without Ca supplement, compared to the control diet (P<0.05). Meat color, pH and water-holding capacity in longissimus muscle were not different between the two groups. The Warner-Brazler Shear (WBS) force of the longissimus muscle was slightly (P=0.08) lower in steers fed the diet without Ca supplement than in steers fed the control diet (2.9 vs 3.2 kg/1.27-cm diameter core). Sensory evaluation showed that feeding the diet without Ca supplement slightly (P<0.05) improved tenderness (4.9 vs 4.5) and flavor (4.9 vs 4.6), compared to feeding the control diet, but juiciness was not affected by diets. Results showed that deletion of Ca supplement from finishing diets is beneficial, increasing growth and marbling partly through an increased energy intake and induced 1,25-dihydroxy vitamin $D_3$ synthesis that may increase intracellular $Ca^{2+}$ concentration and in turn fat synthesis.

• Journal of Nutrition and Health
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• v.32 no.6
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• pp.661-670
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• 1999

Effects of Ca supplementation on blood pressure(BP) response to dietary Na level were studied in 15 normotensive healthy college women with family history of hypertension. All subjects, randomly divided into 3 groups, ate low Na diet(1816mg/day) prepared in the laboratory during the first 2 weeks and normal Na diet(4064mg/day) of their own home for the next 8 weeks. The one group received daily 1g Ca supplement at both low and normal Na diet periods, the second group took daily 1g Ca supplement only at normal a diet period, and the last group took placebo during both periods. Average Ca and energy intakes at the basal of and during the trial ranged 450-600mg and 1735-1878kcal, respectively. Systolic/diastolic BP was decreased by 9.2-9.8/4.4-4.5 mmHg during low Na diet period and was increased again during next normal Na diet period. However simultaneous Ca supplementation during both low and normal Na diet period suppressed the elevation of systolic/diastolic BP occurred at normal Na diet period. Ca supplementation only at normal Na diet peroid did not affect the BP elevation. The increase of serum Ca and Ca/Mg ratio and the decrease of serum Na and Na/K ratio might be related to the BP lowering effects of Ca supplementation. In conclusion, Ca supplement could attenuate BP elevation induced by increasing Na intake. The BP lowering effect of Ca supplementation was not appeared at low Na intake. Further studies were needed to make it certain. Low Na intake was also confirmed as an effective diet control for lowering blood pressure.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.6
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• pp.806-817
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• 2014

The objective of this experiment was to evaluate the effect of providing different levels of a supplement on the nutritional characteristics and productive performance of heifers on pasture during the rainy-dry transition and dry season in Brazil or tropical area. Thirty crossbred heifers with predominance of Zebu breed were used in a completely randomized experimental design. Treatments consisted of a mineral supplement and 0.5, 1.0, 1.5, or 2.0 kg/animal/d of a protein supplement containing 300 g crude protein (CP)/kg of dry matter (DM). In the rainy-dry transition season there was quadratic effect of the protein supplementation (p<0.10) on daily weight gain (DWG). A linear relationship (p<0.10) was found between increasing supplement intake and intakes of DM, organic matter (OM), crude protein (CP), ether extract (EE), non fibrous carbohydrates (NFC) and total digestible nutrients (TDN). Coefficients of apparent digestibility of CP, EE, and NFC increased linearly (p<0.10) with increasing supplement levels, but there was no effect on the DM apparent digestibility (p>0.10); the microbial efficiency (g CPmic/kg TDN) and the relationship of microbial nitrogen flow with nitrogen intake (g/g nitrogen intake) were negative linear profiles. In the dry season, the descriptive pattern least squares means showed a trend of stabilization of DWG from the supply of 0.98 kg of protein supplement; the intakes of DM, OM, CP, EE, NFC, and TDN showed increasing linear relationship (p<0.10) with protein supplement levels; the means of apparent digestibility coefficients of the different dietary fractions presented a linear-response-plateau (LRP); the microbial nitrogen flow (g/d) showed positive linear profile (p<0.10) for supplementation levels. It is concluded that supplementation improves the productive performance of grazing heifers and that 1.0 kg/d of supplement per animal gives the maximum increment of weight gain.

• Journal of Nutrition and Health
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• v.33 no.2
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• pp.147-157
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• 2000

This study examined the effects of excess intake of calcium (Ca) and iron (Fe) supplement on bone loss, nephrocalcinosis and renal function in osteoporotic model rats. Seven-week-old female rats were first fed a Ca-deficient diet for four weeks after ovariectomy operation, and then one of nine experimental diets for additional eight weeks, containing three levels of Ca, normal (0.5%) or high (1.5%) or excess (2.5%) and three levels of Fe, normal (35ppm) or high (210ppm) or excess (350ppm). The osteoporotic model rats showed a remarkable increase in body weight, serum alkaline phosphatase (ALP) and decrease in breaking force, Ca, P, Mg contents of femur. Serum Ca concentration was not significantly affected by dietary Ca and Fe levles. Liver Ca content increased in rats fed a high-and excess-Ca diet. Kidney Ca content and microscopic Ca deposition remarkably increased in osteoporotic model rats compared to control group, and showed a tendency to decrease in rats fed a excess-Ca diet. Breaking force of femur increased with increasing dietary Ca levels, but Ca, P contents of femur and serum ALP were not significantly affected by dietary Ca and Fe levels. Serum total protein decreased in rats fed a excess-Ca diet, BUN increased in rats fed a excess-Ca diet, while serum uric acid and creatinine were not significantly affected by dietary Ca levels. Urinary creatinine, GFR increased in rats fed a high-and excess-Ca, diet, and GFR was highest in rats fed a excess-Ca/excess-Fe diet. These results suggest that excess intake of Ca may increase breaking force of femur, but not increase mineral contents of femur, and decrease kidney function. Furthermore, excess intake of Fe and Ca concurrently may aggravate kidney function leading to potential health problems in ovariectomized osteoporotic model rats.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.9
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• pp.1278-1287
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• 2002

To record the prevalence of macro-minerals deficiency in buffaloes, a survey was conducted in certain parts of Northern India. The prevalence of soil Ca, P, Mg, Na, P and K deficiency was 21.35%, 23.30%, 28.64%, 3.61% and 6.84%, respectively while that of fodder Ca, P, Mg, Na and K deficiency was 13.88%, 16.55%, 19.72%, 3.54% and 4.86%, respectively. The overall prevalence of serum (buffalo) Ca, P, Mg, Na and K deficiency in certain parts of northern India was 25.48%, 24.66%, 24.36%, 4.42% and 3.28%, respectively. The correlation coefficient of Ca, P, Mg, Na and K in soil, fodder and serum was significant and in most of the cases the values were above 0.6. The highest deficiency of macro-minerals i.e. Ca, P, Mg, Na and K was found in plain regions, followed by Tarai (foot hill of Himalayas) region and finally the hilly region. For therapeutic studies, three types of mineral mixture were prepared according to deficiency obtained and fed to three groups of deficient animals. Observations were recorded on 0, 30, 60 and 75 day. In group A animals normal mineral mixture was provided, where as in group C and D 10% and 25% more of Ca, P, Mg were provided, respectively. There was an increase in body weight, milk yield, haemoglobin concentration, and total erythrocyte count. Alanine aminotransferase, aspartate amino transferase in group D animals. There was a decrease in heart rate, respiratory rate and alkaline phosphatase in group D animal after mineral supplement. Thus showing the efficacy when supplements 3 provided to group D animals.

• Journal of the Korean Society of Food Science and Nutrition
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• v.29 no.4
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• pp.743-746
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• 2000

The possiblity of sepiae os as a calcium supplement in food was studied. Elementary contents of sepiae os were as follows; Ca 53.254%, O 26.781%, Na 14.905%, Cl 4.37%, Sr 0.507%, P 0.068% and Si 0.051%, respectively. Most of calcium in sepiae os was present as a form of CaCO3. Sruface area of sepiae os was found as 386 m2/g, and scanning electron micrograph showed sepiae os has multilayer struture. Buffering capacity of sepiae os that of CaCO3. With above results, sepiae os can be used as a calcium supplement in food with good chacterateristics.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.5
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• pp.643-650
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• 2009

Two metabolism trials were conducted with 24 wether lambs to investigate the effects of feeding crab meal and other protein supplements on N utilization, digestibility and Ca and P balance in sheep. The lambs (avg. BW, 25 kg) were randomly allotted to eight diets in each of two trials. The supplements were: i) none, negative control (NC); ii) soybean meal (SBM), control; iii) supplement based on industrial byproducts of both plant and animal origin (IPA); iv) experimental supplement based on byproducts of animal origin (ESA); v) hydrolyzed supplement No 4. (HESA); vi) commercial supplement based on animal protein (CS), $Pro-Lak^{(R)}$ vii) crab meal (CM); and viii) urea (U). The supplements supplied 33% of the total dietary N (CP, 9.8%; DM basis). Lambs fed the NC diet had lower (p<0.05) DM and OM digestibility. Lower (p<0.05) apparent absorption of N was recorded for the lambs fed the HESA and NC diets. Sheep fed CM had lower Ca absorption compared to SBM. Highest (p<0.05) P absorption was observed for lambs fed CS and CM and lowest for U and NC diets. Sheep fed CM had higher (p<0.05) total VFA concentration (65.7 ${\mu}mol/ml$), compared to those fed ESA, CS, and NC diets (47.3, 49.8, and 49.5 ${\mu}mol/ml$, respectively). Highest (p<0.05) ruminal $NH_3$ N (29.6 mg/dl) was observed in lambs fed the U diet, while those fed the NC diet had the lowest (p<0.05) average value (7.66 mg/dl). Lambs fed the U diet had the highest (p<0.05) blood urea N (10.67 mg/dl). The present study showed that N utilization of diets supplemented with experimental supplements based on feather meal and blood meal; commercial supplement based on animal protein, $Prolak^{(R)}$ supplement based on plant protein and blood meal; and crab meal are comparable with that of soybean meal.

• Journal of Nutrition and Health
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• v.41 no.3
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• pp.206-215
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• 2008

The purpose of this study was to investigate the effect of manganese (Mn) supplementation on bone status and calcium balance in ovariectomized rats according to the calcium intake levels. Total of 50 Sprague Dawley female rats (6 weeks) were divided into 5 groups and bred for 12 weeks: sham operated control group (SACa), OVX Ca deficiency group (OLCa) with Ca deficiency diet (0.1% Ca modified AIN-93N diet), OVX Ca deficiency & Mn supplement group (OLCaMn), OVX adequate Ca group (OACa; 0.5% Ca AIN-93N diet) and OVX adequate Ca & Mn supplement group (OACaMn). BMD (bone mineral density) of the femur was increased by Mn supplementation in OVX adequate Ca group. However, BMDs of spine, femur and tibia were lowered in OLCa compared to the OLCaMn group. Bone strength of tibia in OLCaMn group was significantly lower than OLCa group. Serum ALP (alkaline phosphatase) and CTx (C-telopeptide of collagen cross-links) levels were significantly higher in ovariectomized rats than those in the sham group, but they were not changed by Mn supplementation. Ca retention rate and Ca absorption rate did not differ among the experimental groups. Urinary Ca excretion was increased by Mn supplementation in Ca deficiency rats. In summary, Mn supplementation resulted in positive effects on bone mineral density ovariectomized rats with which intake adequate Ca. However, Mn supplementation on Ca deficiency ovariectomized rats resulted in decrement of BMO and bone strength by increasing Ca excretion. Therefore, it is encouraged to consider calcium intake levels in supplementation of manganese in order to prevent postmenopausal osteoporosis and to keep bone healthy. (KoreanJNutr2008; 41(3): 206~215)

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.5
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• pp.637-647
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• 2019

Objective: The study aimed at quantifying seasonal and spatial variations in availability and nutritive value of herbaceous vegetation on native pastures and supplement feedstuffs for domestic ruminants in Western Kenya. Methods: Samples of herbaceous pasture vegetation (n = 75) and local supplement feedstuffs (n = 46) for cattle, sheep, and goats were collected in 20 villages of three geographic zones (Highlands, Mid-slopes, Lowlands) in Lower Nyando, Western Kenya, over four seasons of one year. Concentrations of dry matter (DM), crude ash (CA), ether extract (EE), crude protein (CP), neutral detergent fibre (NDF), gross energy (GE), and minerals were determined. Apparent total tract organic matter digestibility (dOM) was estimated from in vitro gas production and proximate nutrient concentrations or chemical composition alone using published prediction equations. Results: Nutrient, energy, and mineral concentrations were 52 to 168 g CA, 367 to 741 g NDF, 32 to 140 g CP, 6 to 45 g EE, 14.5 to 18.8 MJ GE, 7.0 to 54.2 g potassium, 0.01 to 0.47 g sodium, 136 to 1825 mg iron, and 0.07 to 0.52 mg selenium/kg DM. The dOM was 416 to 650 g/kg organic matter but differed depending on the estimation method. Nutritive value of pasture herbage was superior to most supplement feedstuffs, but its value strongly declined in the driest season. Biomass yields and concentrations of CP and potassium in pasture herbage were highest in the Highlands amongst the three zones. Conclusion: Availability and nutritive value of pasture herbage and supplement feedstuffs greatly vary between seasons and geographical zones, suggesting need for season- and region-specific feeding strategies. Local supplement feedstuffs partly compensate for nutritional deficiencies. However, equations to accurately predict dOM and improved knowledge on nutritional characteristics of tropical ruminant feedstuffs are needed to enhance livestock production in this and similar environments.