• Title/Summary/Keyword: CRUD

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Component proteins in crud extract of adult Paragonimus westermani purified by immunoaffinity chromatography using monoclonal antibodes (친화성크로마토그래피로 순수분리한 폐흡충 성충 성분단백질의 성상)

  • Kang, Shin-Yong;Kong, Yoon;Cho, Seung-Yull
    • Parasites, Hosts and Diseases
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    • v.29 no.4
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    • pp.363-370
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    • 1991
  • 인체감염이 다발하는 폐흡충증의 혈청학적 진단에서 항원으로 사용하는 성충추출액은 여러단계의 질환 이행과정을 진단하는 항원으로서 문제가 있다. 이를 해결하려면 먼저 추출액내의 성분단백질의 성상을 파악할 필요가 있다. 이 연구에서는 폐흡충 성충 추출액으로 면역시킨 BALB/c mice의 비장세포와 SP2/0 형질세포종 세포를 세포융합하여 제작한 단세포군항체를 이용하여 친화성크로마토그래피로 폐흡충 성충의 구성단백질의 일부를 순수분리하고 성상을 관찰하였다. 그 결과는 다음과 같다. 1. 세포융합으로 PFCK-21, PFCK-44, PFCK-136, PFCK-189 등 4종류의 단세포군 항체를 얻었다. 그중 PFCK-21과 PFCK44는 17k Da, PFCK-136은 23, 46, 92 kDa 단백질에 반응하였고 PFCK-189는 여러종류의 단백질에 반응하였다. 2. PFCK-44 단세포군항체를 고리로 친화성 크로마토그래피를 실시하여 분리한 성분 단백질은 disc-PAGE상 4번째에 위치하고 분자량이 17 kDa로 알려진 단밸질이었다. 이 단밸질은 17 kDa의 monomer로 판단하였다. 면역조직화학염색을 실시한 결과 이 단밸질은 장관 상피세포에 반응하고 있었다. 3. PFCK-136 단세포군항체로 순수분리한 단밸질은 disc-PAGE상 1번 단밸질(440 kDa)이었으며 환원성 SDS-PAGE에서는 23 kDa 단밸질이었다. 면역조직화학염색으로 이 단세포군항체는 충란내 세포에 강하게 반응하고 있었다.

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Study on multi-stage magnetic separation device for paramagnetic materials operated in low magnetic fields

  • F. Mishima;Aoi Nagahama;N. Nomura;S. Nishijima
    • Progress in Superconductivity and Cryogenics
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    • v.25 no.3
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    • pp.13-17
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    • 2023
  • Magnetic separation technology for small paramagnetic particles has been desired for the volume reduction of contaminated soil from the Fukushima nuclear power plant accident and for the separation of scale and crud from nuclear power plants. However, the magnetic separation for paramagnetic particles requires a superconducting high gradient magnetic separation system applied, hence expanding the bore diameter of the magnets is necessary for mass processing and the initial and running costs would be enormous. The use of high magnetic fields makes safe onsite operation difficult, and there is an industrial need to increase the magnetic separation efficiency for paramagnetic particles in as low a magnetic field as possible. Therefore, we have been developing a magnetic separation system combined with a selection tube, which can separate small paramagnetic particles in a low magnetic field. In the previous technique we developed, a certain range of particle size was classified, and the classified particles were captured by magnetic separation. In this new approach, the fluid control method has been improved in order to the selectively classify particles of various diameters by using a multi-stage selection tube. The soil classification using a multi-stage selection tube was studied by calculation and experiment, and good results were obtained. In this paper, we report the effectiveness of the multi-stage selection tube was examined.

Development of ALARA Checklist for an ALARA Design Review (ALARA 설계검토를 위한 ALARA 점검표 개발)

  • Shin, Sang-Woon;Song, Myung-Jae
    • Journal of Radiation Protection and Research
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    • v.21 no.3
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    • pp.155-166
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    • 1996
  • All nuclear facilities and components should receive an initial ALARA review before the installation and thereafter whenever modifications are planned. A major objective in design aspects of ALARA is to identify areas where specific engineering input can reduce personnel exposure. The basic factors which should be considered in the ALARA design review process include curd control, shielding and isolation of radiation sources accessibility maintainability and reliability, and contamination control. Because many diverse aspects must be considered in the ALARA design reviews, a proper ALARA checklist should be used to aid the designer in preventing any of the ALARA review considerations from being slipped away. In order to develop the practical ALARA checklist, check items for basic ALARA factors have been prepared, and what should be considered in reviewing each item has been discussed here. Based on the proposed factors and items, an ALARA checklist was developed.

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Characteristics in Tissue Cultured Plants of Chinese foxglove(Rehmannia glutinosa) (지황 조직배양주의 수량성과 성분함량 특성)

  • 박충헌;성낙술;김선규;백기엽
    • Korean Journal of Plant Tissue Culture
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    • v.26 no.3
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    • pp.205-209
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    • 1999
  • Chinese foxglove(Rehmannia glutinosa) is receiving much attention as one of the principal medicinal crops and the crud drug. This study was conducted to obtain the basic breeding information of Chinese foxglove derived from tissue culture. To compare plant characteristics between local variety and tissue cultured seedlings, plant growth and root yield has been investigated. In addition, catalpol and free sugar contents were also analized. The ability for the storage of root stock originated from tissue culture seedlings were better than that of local variety. The growth and root yield of in vitro propagated plants were superior to those of conventionally propagated plants. Root yield of 1-year-old and 2-year-old seedlings investigated 112% and 246% respectively than Seacheon local shows 384 kg/10 a. Although there were no significant different between tissue culture seedlings and conventionally propagated one, slight decline of component contents in tissue culture plants were still existed. Investigated sugar content among Seocheon local, 1-year-old tissue culture seedlings, and 2-year-old tissue culture seedlings were 1.86%, 1.21%, and 1.10% respectively. Catalpol content as one of standard materials indicates 0.46% in Seocheon local and 0.40% in tissue cultured ones.

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Effect of Seeding Date on Forage Yield and Chemical Composition of Echinochloa crusgalli var. Frumentacea(Roxb) Wight in Jeju Region (제주지역에서 파종기에 따른 청예피의 사료수량 및 조성분 변화)

  • 조남기;강영길;송창길;고영순;조영일
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.21 no.4
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    • pp.217-224
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    • 2001
  • In order to identify the growth characterization, the yield and chemical composition of Jeju barnyard grass (Echinochloa crusgalli var. fiurnentacea(Roxb) Wight) based on seeding date in Jeju region, seeding carried out the 10-day intervals from March 27 to September 30 in 2000, respectively. Plant height was 143.2 cm, showing the highest on seeding date, April 6 among that of any other seeding date. On the other hand, those of early and late seeding gradually decrease. Plant height was 119.2 an in May 16 seeding. The results of stem diameter, number of withering leaves, number of leaves and fresh weight per plant were similar to those of the plant heights. The yield of fresh, dry matter forage, crude protein and TDN found the highest on April 6 seeding, 63.5 MT/ha, 13.9 MTha, 1.1 MT/ha, and 7.6 MT/ha, respectively. In early and late seeding, the yield was gradually decreased. In seeding May 16, the yield found .38.2 MTIha in fresh forage, 6.2 MTha in dry matter forage, 0.7 MT/ha in crude protein and 3.7 MTha in TDN, respectively. According to delaying the seeding date, March 27 to May 16, the contents of crude protein (from 7.9 to 10.8%), ether extract (from 4.6 to 6.0%), nitrogen free extract (from 45.1 to 46.5%), and TDN (from 54.2 to 60.8%) were gradually increased, respectively. On the other hand, those of crude fiber (from 28.9 to 25.6%) and crud ash (from 13.5 to 11.2%) were decreased. These results showed that April 6 was the optimum seeding date with the sole object of feed production of Jeju barnyard grass under the environmental condition like as atmospheric phenomena and soil in Jeju region. (Key words : Jeju barnyard grass, Seeding date, Forage yield, Chemical composition)

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Implement of Smart Phone Application for Website Administration (스마트폰을 이용한 모바일 웹사이트 관리 프로그램 구현)

  • Park, Suhyun;Park, Jaeyang
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2009.05a
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    • pp.876-879
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    • 2009
  • As performance and feature set of mobile devices are getting better, the scope of things for which they can be used is also getting widened. More than simply browsing information using mobile web, now these devices can interact with users. It requires various features to manage a web site; supporting full-browsing alone is not enough since there are problems such as processing speed, user interface restriction. However, using mobile devices to manage a web site has its own merits since the can be used without restriction of time and location. In this paper, we introduce a mobile web site administration software that provides both online and offline features. It supports CRUD (Create, Read, Update, Delete) operations for news and users. These operations can be executed both offline and online. A user can do most of things offline; It connects to online only when it's absolutely necessary and does those in batch mode.

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MEASUREMENT OF NUCLEAR FUEL ROD DEFORMATION USING AN IMAGE PROCESSING TECHNIQUE

  • Cho, Jai-Wan;Choi, Young-Soo;Jeong, Kyung-Min;Shin, Jung-Cheol
    • Nuclear Engineering and Technology
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    • v.43 no.2
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    • pp.133-140
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    • 2011
  • In this paper, a deformation measurement technology for nuclear fuel rods is proposed. The deformation measurement system includes a high-definition CMOS image sensor, a lens, a semiconductor laser line beam marker, and optical and mechanical accessories. The basic idea of the proposed deformation measurement system is to illuminate the outer surface of a fuel rod with a collimated laser line beam at an angle of 45 degrees or higher. For this method, it is assumed that a nuclear fuel rod and the optical axis of the image sensor for observing the rod are vertically composed. The relative motion of the fuel rod in the horizontal direction causes the illuminated laser line beam to move vertically along the surface of the fuel rod. The resulting change of the laser line beam position on the surface of the fuel rod is imaged as a parabolic beam in the high-definition CMOS image sensor. An ellipse model is then extracted from the parabolic beam pattern. The center coordinates of the ellipse model are taken as the feature of the deformed fuel rod. The vertical offset of the feature point of the nuclear fuel rod is derived based on the displacement of the offset in the horizontal direction. Based on the experimental results for a nuclear fuel rod sample with a formation of surface crud, an inspection resolution of 50 ${\mu}m$ is achieved using the proposed method. In terms of the degree of precision, this inspection resolution is an improvement of more than 300% from a 150 ${\mu}m$ resolution, which is the conventional measurement criteria required for the deformation of neutron irradiated fuel rods.

Characteristics of Yellow Layer Cake Made with Mandarin Powder (밀감 분말을 첨가한 옐로우 레이어 케이크의 품질특성)

  • Park, Yeong-Sun;Shin, Sol;Shin, Gil-Man
    • Food Science and Preservation
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    • v.15 no.5
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    • pp.656-661
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    • 2008
  • basic formulation. Moisture content of 13.72%, crude protein 5.22%, crude lipid 1.31%, crud ash 1.94%, respectively. The pH of yellow layer cake decreased with increasing mandarin powder concentrations. In color values, with increase of mandarin powder concentration. The volume decreased with increasing mandarin powder concentration. The weights of yellow layer cake increased with increasing mandarin powder concentration. L value and a value decreased with increasing mandarin powder concentration in the crumb. b value increased with increasing mandarin powder concentration. The baking loss rate decreased with increasing mandarin powder. In the texture hardness, gumminess, chewiness and adhesiveness of yellow layer cakes significantly increased with increasing mandarin powder concentration. springiness and cohesiveness of yellow layer cakes significantly decreased. In the results of sensory evaluation, when compared to the control, the bread added to 9% of mandarin powder was superior to in color and flavor, while was similar to texture and taste.

Experimental investigation of two-phase flow and wall heat transfer during reflood of single rod heater (단일 가열봉의 재관수 시 2상유동 및 벽면 열전달에 관한 실험적 연구)

  • Park, Youngjae;Kim, Hyungdae
    • Journal of the Korean Society of Visualization
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    • v.18 no.3
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    • pp.23-34
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    • 2020
  • Two-phase flow and heat transfer characteristics during the reflood phase of a single heated rod in the KHU reflood experimental facility were examined. Two-phase flow behavior during the reflooding experiment was carefully visualized along with transient temperature measurement at a point inside the heated rod. By numerically solving one-dimensional inverse heat conduction equation using the measured temperature data, time-resolved wall heat flux and temperature histories at the interface of the heated rod and coolant were obtained. Once water coolant was injected into the test section from the bottom to reflood the heated rod of >700℃, vast vapor bubbles and droplets were generated near the reflood front and dispersed flow film boiling consisted of continuous vapor flow and tiny liquid droplets appeared in the upper part. Following the dispersed flow film boiling, inverted annular/slug/churn flow film boiling regimes were sequentially observed and the wall temperature gradually decreased. When so-called minimum film boiling temperature reached, the stable vapor film between the heated rod and coolant was suddenly collapsed, resulting in the quenching transition from film boiling into nucleate boiling. The moving speed of the quench front measured in the present study showed a good agreement with prediction by a correlation in literature. The obtained results revealed that typical two-phase flow and heat transfer behaviors during the reflood phase of overheated fuel rods in light water nuclear reactors are well reproduced in the KHU facility. Thus, the verified reflood experimental facility can be used to explore the effects of other affecting parameters, such as CRUD, on the reflood heat transfer behaviors in practical nuclear reactors.

Effects of Seed Mixture and Nitrogen Levels on Botanical Composition and Forage Productivity for Pasture in Jeju (제주지역 초지에서 혼파조합 및 질소수준이 식생구성 및 사초생산성에 미치는 영향)

  • Park, H.S.;Hwang, K.J.;Park, N.G.;Kim, W.H.;Lee, J.K.;Kim, J.G.;Lee, K.W.;Lim, Y.C.
    • Journal of Animal Environmental Science
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    • v.15 no.3
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    • pp.263-270
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    • 2009
  • A study was conducted to determine the effects of seed mixture and nitrogen application levels on botanical composition and forage productivity in grazing pasture. Legume forage have the ability to take nitrogen from the atmosphere and convert it into a form usable by plants. Including legumes in mixtures with grass lowers the amount of nitrogen fertilizer required to produce forages. Dry matter (DM) yield of grass-legume mixtures was more than that of grass mono-cultivated and grass+white clover+red clover mixtures was the highest as 17,391 kg/ha in legume mixture (P<0.01). The highest DM yield was in N-200 kg/ha, but it was similar between N-100 kg/ha(15,128) and N-200 kg/ha (16,017). The large decline in the proportion of grass during April to June in 2004 probably was due to the drought and summer depression in grass-legume mixtures. Grass proportions decreased by 8.2% after 2 year, and white clover was dominated in grass-white clover mixture by May 2005. Crud protein (CP) content tends to increase with increased nitrogen application level.

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