• Bulletin of the Korean Chemical Society
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• v.34 no.9
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• pp.2725-2730
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• 2013

Single C-reactive protein (CRP) molecules, which are non-specific acute phase markers and products of the innate immune system, were quantitatively detected on a gold-nanopatterned biochip using evanescent field-enhanced fluorescence imaging. The $4{\times}5$ gold-nanopatterned biochip (spot diameter of 500 nm) was fabricated by electron beam nanolithography. Unlabeled CRP molecules in human serum were identified with single-molecule sandwich immunoassay by detecting secondary fluorescence generated by total internal reflection fluorescence (TIRF) microscopy. With decreased standard CRP concentrations, relative fluorescence intensities reduced in the range of 33.3 zM-800 pM. To enhance fluorescence intensities in TIRF images, the distance between biochip surface and CRP molecules was optimally adjusted by considering the quenching effect of gold and the evanescent field intensity. As a result, TIRF only detected one single-CRP molecule on the biochip the first time.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.21 no.2
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• pp.283-289
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• 2012

Cam rack pinion (CRP) system which consists of cam rack and roller pinion transforms the rotation motion into linear one. The roller pinion has the plurality of rollers and meshes with its conjugated cam rack. The exact tooth profile of the cam rack and the non-undercut condition to satisfy the required performance have been proposed by introducing the profile shift coefficient. The load stress factors are investigated by varying the shape design parameters to predict the gear surface fatigue limit which is strongly related to the gear noise and vibration at the contact patch. The results show that the pitting life can be extended significantly by increasing the profile shift coefficient.

• Mycobiology
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• v.46 no.4
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• pp.429-439
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• 2018

To develop a convenient promoter analysis system for fungi, a null-pigment mutant (NPG) of Aspergillus nidulans was used with the 4'-phosphopantetheinyl transferase (PPTase) gene, npgA, which restores the normal pigmentation in A. nidulans, as a new reporter gene. The functional organization of serially deleted promoter regions of the A. nidulans trpC gene and the Cryphonectria parasitica crp gene in filamentous fungi was representatively investigated to establish a novel fungal promoter assay system that depends on color complementation of the NPG mutant with the PPTase npgA gene. Several promoter regions of the trpC and crp genes were fused to the npgA gene containing the 1,034-bp open reading frame and the 966-bp 3' downstream region from the TAA, and the constructed fusions were introduced into the NPG mutant in A. nidulans to evaluate color recovery due to the transcriptional activity of the sequence elements. Serial deletion of the trpC and crp promoter regions in this PPTase reporter assay system reaffirmed results in previous reports by using the fungal transformation step without a laborious verification process. This approach suggests a more rapid and convenient system than conventional analyses for fungal gene expression studies.

• Tuberculosis and Respiratory Diseases
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• v.58 no.6
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• pp.562-569
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• 2005

Background : The severity scoring system is useful for predicting the outcome of critically ill patients. However, the system is quite complicated and cost-ineffective. Simple serologic markers have been proposed to predict the outcome, which include troponin-I, lactate and C-reactive protein(CRP). The aim of this study was to evaluate the prognostic values of troponin-I, lactate and CRP in critically ill non-cardiac patients. Methods : From September 2003 to June 2004, 139 patients(Age: $63.3{\pm}14.7$, M:F = 88:51), who were admitted to the MICU with non-cardiac critical illness at Gyeongsang National University Hospital, were enrolled in this study. This study evaluated the severity of the illness and the multi-organ failure score (Acute Physiologic and Chronic Health EvaluationII, Simplified Acute Physiologic ScoreII and Sequential Organ Failure Assessment) and measured the troponin-I, lactate and CRP within 24 hours after admission in the MICU. Each value in the survivors and non-survivors was compared at the 10th and 30th day after ICU admission. The mortality rate was compared at 10th and 30th day in normal and abnormal group. In addition, the correlations between each value and the severity score were assessed. Results : There were significantly higher troponin-I and CRP levels, not lactate, in the non-survivors than in the survivors at 10th day($1.018{\pm}2.58ng/ml$, $98.48{\pm}69.24mg/L$ vs. $4.208{\pm}10.23ng/ml$, $137.69{\pm}70.18mg/L$) (p<0.05). There were significantly higher troponin-I, lactate and CRP levels in the non-survivors than in the survivors on the 30th day ($0.99{\pm}2.66ng/ml$, $8.02{\pm}9.54ng/dl$, $96.87{\pm}68.83mg/L$ vs. $3.36{\pm}8.74ng/ml$, $15.42{\pm}20.57ng/dl$, $131.28{\pm}71.23mg/L$) (p<0.05). The mortality rate was significantly higher in the abnormal group of troponin-I, lactate and CRP than in the normal group of troponin-I, lactate and CRP at 10th day(28.1%, 31.6%, 18.9% vs. 11.0%, 15.8 %, 0%) and 30th day(38.6%, 47.4%, 25.8% vs. 15.9%, 21.7%, 14.3%) (p<0.05). Troponin-I and lactate were significantly correlated with the SAPS II score($r^2=0.254$, 0.365, p<0.05). Conclusion : Measuring the troponin-I, lactate and CRP levels upon admission may be useful for predicting the outcome of critically ill non-cardiac patients.

• Journal of rehabilitation welfare engineering & assistive technology
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• v.10 no.2
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• pp.147-153
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• 2016

The aims of this study were to evaluate the joint coordination between hip and knee joints in the transfemoral amputees, and its symmetry between sound and prosthetic limbs. Seven transfemoral amputees ($46.4{\pm}10.7-year-old$, $174.8{\pm}3.5cm$, $78.3{\pm}9.7kg$) and 7 able bodies ($24.0{\pm}4.5-year-old$, $174.5{\pm}5.9cm$, $66.9{\pm}9.4Kg$) participated in this study. They walked at a self-selected walking speed across a 10m level ground. Simultaneously angle and angular velocity in the hip and knee joint were measured by motion analysis system. Then continuos relative phase(CRP), standard deviations of CRP (CRP_SD) and symmetry index(SI) were calculated. In able bodies, there were no differences of the parameters between left and right limb(all p>0.05). However, significant differences between sound and prosthetic limb in most of the parameters, except for CRP for stance phase were shown (all p<0.05). There were differences in all SI between transfemoral amputees and able bodies (p<0.05). In conclusion, joint coordination was altered in transfemoral amputee during level walking and shown in interlimb asymmetry.

• Journal of Korean Society of Transportation
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• v.31 no.4
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• pp.85-94
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• 2013

The Continuous Risk Profile (CRP) has been well known to be the most accurate and efficient among existing network screening methods. However, the classical CRP uses safety performance functions (SPFs) which require a huge investment to construct a database system. This study aims to suggest a new CRP method using average crash frequencies of homogeneous groups, instead of SPFs, as rescaling factors. Hierarchical clustering analysis is performed to classify freeway segments into homogeneous groups based on the data of AADT and number of lanes. Using the data from I-880 in California, the proposed method is compared to other several network screening methods. The results show that the proposed method decrease false positive rates while it does not produce any false negatives. The method developed in this study can be easily applied to screen freeway networks without any additional complex database systems, and contribute to the improvement of freeway safety management systems.

• International Journal of Naval Architecture and Ocean Engineering
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• v.7 no.3
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• pp.595-609
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• 2015

The wake characteristics of Contra-Rotating Propeller (CRP) were investigated using numerical simulation and flow measurement. The numerical simulation was carried out with a commercial CFD code based on a Reynolds Averaged Navier-Stokes (RANS) equations solver, and the flow measurement was performed with Stereoscopic Particle Image Velocimetry (SPIV) system. The simulation results were validated through the comparison with the experiment results measured around the leading edge of rudder to investigate the effect of propeller operation under the conditions without propeller, with forward propeller alone, and with both forward and aft propellers. The evolution of CRP wake was analyzed through velocity and vorticity contours on three transverse planes and one longitudinal plane based on CFD results. The trajectories of propeller tip vortex core in the cases with and without aft propeller were also compared, and larger wake contraction with CRP was confirmed.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.23 no.2
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• pp.194-198
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• 2014

In recent years, RGM(roller gear mechanism) systems, wherein one of the gears of a meshing gear pair is replaced with pins or rollers, have been reintroduced, which is a consequence of, and therefore a reflection of, the rapid advances made in manufacturing technology. Three RTG(roller track gear) systems for arbitrary path transportation (e.g., L-, O-, U-, and S-shaped tracks) were constructed using two out of three RGM systems, namely, the CRP(cam rack pinion), CRG(cam ring gear), and RPG(roller pinion gear) systems, and are introduced in this paper. We also present three ways to prevent the intersection and non-contact phenomena at the teeth in the vicinity of the conversion point between two joined RGM systems.

• Progress in Superconductivity and Cryogenics
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• v.18 no.4
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• pp.25-29
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• 2016

The high temperature superconducting (HTS) contra-rotating propulsion (CRP) systems comprise two coaxial propellers sited on behind the other and rotate in opposite directions. They have the hydrodynamic advantage of recovering the slipstream rotational energy which would otherwise be lost to a conventional single-screw system. However, the cooling systems used for HTS CRP system need a high cooling power enough to maintain a low temperature of 2G HTS material operating at liquid neon (LNe) temperature (24.5 - 27 K). In this paper, a single thermo-syphon cooling approach using a Gifford-McMahon (G-M) cryo-cooler is presented. First, an optimal thermal design of a 1.5 MW HTS motor was conducted varying to different types of commercial 2G HTS tapes. Then, a mono-cryogenic cooling system for an integration of two 1.5 MW HTS motors will be designed and analyzed. Finally, the 3D finite element analysis (FEA) simulation of thermal characteristics was also performed.

• The Journal of Korean Physical Therapy
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• v.34 no.6
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• pp.326-331
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• 2022

Purpose: The stroke patients have gait dysfunction due to impaired neural tracts; corticospinal tract (CST), corticoreticular pathway (CRP), and vestibulospinal tract (VST). In this study, we investigated characteristics of gait pattern according to the injury aspect of the neural track in a stroke patient. Methods: One patient and six control subjects of similar age participated. A 19-year-old male patient with spontaneous intracerebral hemorrhage on right basal ganglia, thalamus, corona radiata and cerebral cortex due to arteriovenous malformation rupture. Diffusion tensor imaging (DTI) data was acquired 21 months after the stroke. Kinematic and spatio-temporal parameters of gait were collected using a three-dimensional gait analysis system. Results: On 21 months DTI, the CST and CRP in affected hemisphere showed severe injury, in contrast, the VST in affected hemisphere showed intact integrity. Result of gait analysis, walking distance and speed were significantly decreased in a patient. The stance rate of unaffected lower limb, the swing rate of affected lower limb and the duration of double stance significantly increased compared with normal control. The knee and hip joint angle were significantly decreased in a patient. Conclusion: We found recovered independent gait ability may be associated with unimpaired VST in a patient with severe injury in CST and CRP.