• 정보보호학회논문지
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• 제13권4호
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• pp.99-107
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• 2003

기존 상호인증 모델이 이론적인 모델로써 보다 구체적인 실제 상호인증에 필요한 많은 부분들이 정의되지 않거나 구체적으로 표준을 어떻게 사용하여야 하는지에 대하여 명확히 설명하지 못하여 이를 해결하기 위해서 상호인증, 인증서를 통한 국제상호인증 모델을 제안하고자 한다. 이를 위해서 인증기관 간 필요한 인증서 프로파일을 제안하고, 이를 보관할 디렉토리 서버에 필요한 스키마를 설계하고, 인증기관이 다른 기관에서 제공하는 저장매체에 손쉽게 접근하도록 하기 위해 PKCS＃11을 통한 표준 방식을 제안하여 확장성과 편리성을 제공하고자 한다. 또한 서로 다른 응용 애플리케이션에서 상호인증서 사용 시 CRL을 통한 인증서 검증을 위한 인증서 경로 획득에 대한 방법을 제안하고자 한다.

• 인터넷정보학회논문지
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• 제7권5호
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• pp.33-41
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• 2006

제 4 세대 이동 통신망 SB12K(Systems Beyond IMI 2000)의 사용화가 예상되고 무선 랜 사용자의 이동서비스(mobile service) 요구가 증가됨에 따라 단말기의 이동성(mobility)을 지원하는 새로운 인증 방법이 요구된다. 무선 랜은 전파라는 전송매체를 사용하기 때문에 보안에 취약하다. 현재 무선 랜의 보안기능을 일관성 있게 제공하는 기술로 WPKI (Wireless Public Key Infrastructure)가 있다. WPKI는 인증서 사용이 필수적인데, 이것은 인증서 검증 및 CRL검색으로 인하여 핸드오프 성능에 많은 부담으로 작용된다. 본 논문에서는 무선 랜의 안정적인 이동 서비스를 위해 WPKI의 기술을 사용하면서 핸드오프에 영향을 주지 않는 기법을 제시한다. 제시한 기법은 통신 데이터의 암호화에 필요한 비밀 키를 단말과 로밍 서버가 선행하여 습득하는 것이다.

• Journal of Communications and Networks
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• 제5권1호
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• pp.25-32
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• 2003

4G mobile communication system requires the throughput of 10-100Mbps. Adaptive modulated OFDM system is promising technique for increasing the throughput. In the pilot symbol assisted high-speed packet transmission system, the data symbol duration is generally considered to be small compared to the coherence time. However, OFDM symbol duration is longer than the symbol duration of a single carrier system, so that the packet duration of the pilot symbol assisted high speed packet transmission system is long. In this case, the change of channel conditions is too fast to be accurately estimated by channel estimator at the receiver in high Doppler frequency, so that many errors occur during demodulation, especially with the data symbols at the end of each packet. In this paper, we consider the BER at various instantaneous $E_b/N_o$ that includes the demodulation errors in high Doppler frequency. When the coherence time is ten times longer than the duration of a single packet, the channel can be closely approximated as an AWGN channel. Otherwise, the approximation breaks down and the above-mentioned errors that occur during demodulation must be taken into consideration. In this paper, we propose the pilot symbol assisted high speed packet transmission system based on adaptive OFDM using a novel lookup table to consider the demodulated errors and evaluate the throughput performance.

• Toxicological Research
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• 제35권4호
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• pp.371-387
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• 2019

Although the dried root of Saposhnikovia divaricata (Turcz.) Schischk. (Umbelliferae) is a popular medicinal plant in East Asia, there has been no systemic toxicological evaluation of a water extract of Saposhnikoviae Radix (SRE). In this experiment, an oral acute and 13-week subchronic toxicological evaluations of SRE (500-5,000 mg/kg body weight) were performed in both sexes of Crl:CD(SD) rats. Based on the results from mortality, clinical signs, effects on body weight and organ weight, clinical biochemistry, hematology, urinalysis, and histopathology, significant acute, 4-week repeated dose range finding (DRF) and 13-week subchronic toxicity of SRE was not observed in either sex of rats; thus, the no observed adverse effect level (NOAEL) was 5,000 mg (kg/day). To identify anti-hyperuricemia potential of SRE, the suppressive effect of SRE was determined in mice challenged with potassium oxonate (PO; 250 mg/kg) via intraperitoneal injection for 8 days (each group; n = 7). SRE supplementation suppressed the uric acid level in urine through significant xanthine oxidase (XO) inhibitory activity. Kidney dysfunctions were observed in PO-challenged mice as evidenced by an increase in serum creatinine level. Whereas, SRE supplementation suppressed it in a dose-dependent manner. Collectively, SRE was safe up to 5,000 mg (kg/day) based on NOAEL found from acute and 13-week subchronic toxicological evaluations. SRE had anti-hyperuricemia effect and lowered the excessive level of uric acid, a potential factor for gout and kidney failure.

• 동의생리병리학회지
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• 제34권4호
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• pp.201-208
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• 2020

The effects of Gamisoyo-san (GMSYS) co-administration within 5 min on the pharmacokinetics (PK) of tamoxifen were observed. After 50 mg/kg of tamoxifen oral treatment, GMSYS 100 mg/kg was orally administered within 5 min to 7-wk old male SPF.VAF Outbred Crl:CD [Sprague-Dawley (SD)] rats. The plasma were collected at 30 min before administration, 30 min, 1, 2, 3, 4, 6, 8 and 24 hrs after end of GMSYS treatment, and plasma concentrations of tamoxifen were analyzed using LC-MS/MS methods. Tmax, Cmax, AUC, t1/2 and MRTinf of tamoxifen were analysis as compared with tamoxifen single administered rats. Although co-administration with GMSYS did not critically influenced on the pharmacokinetic parameters of oral tamoxifen, they induced increased trends of plasma tamoxifen concentrations, especially significant (p<0.05) increases of plasma tamoxifen concentrations were demonstrated at 0.5 hr after end of co-administration with GMSYS as compared with tamoxifen single formula treated rats, at dosage levels of tamoxifen 10 mg/kg and GMSYS 100 mg/kg within 5 min. It is considered that pharmacokinetic studies should be tested like the effects of GMSYS on the pharmacokinetics of tamoxifen, when they were co-administered with prolonger intervals than Tmax of tamoxifen oral administration (about 2.5 hr-intervals), to achieve the optimal dosing regimen of GMSYS and tamoxifen co-administration.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2001년도 춘계학술발표대회
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• pp.7-7
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• 2001

Cryopreservation by vitrification of Hanwoo blastocysts derived from nuclear transfer with Hanwoo adult ear cell was compared with that of in vitro fertilized blastocysts. For vitrification, day 7 or day 8 blastocysts were serially exposed in glycerol (G) or/and ethylene glycol (EG) mixtures (10% G for 5 min, 10% G plus 20% EG for 5 min, and 25% G plus 25% EG for 30 sec) which was diluted in 10% FBS added D-PBS. Thawing of straw was carried out in air for 10 sec and then in water bath of $25^{\circ}C$ for 20 sec. The contents of the straw (0.2 ml) was expelled into a culture dish contained with 0.5M sucrose and 10% FBS(S-DPBS) by cutting the cotton plug and then the recovered embryos were put into fresh 0.25M and 0.125M S-DPBS for 30 sec, respectively, The embryos were transferred into D-PBS with 10% FBS for 5 min. and were cultured in a 10u1 droplet of co-culture environment (cumulus cell monolayer + 10% added CRl medium) for 24 h. In the result, survival rates were 88.9% and 85.4% for nuclear transfer embryos and in vitro fertilized embryos, respectively. After transfer of vitrified-thawed blastocysts produced from nuclear transfer, 4 of 5 total recipients did not return to the subsequent estrus cycle at 30 days. It is concluded that the Hanwoo blastocysts derived from nuclear transfer can be successfully cryopreserved using simple and efficient vitrification method.

• Structural Engineering and Mechanics
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• 제64권5호
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• pp.621-640
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• 2017

This paper reports the numerical investigation conducted to study the influence of Local-Distortional (L-D) interaction mode buckling on post buckling strength erosion in fixed ended lipped channel cold formed steel columns. This investigation comprises of 81 column sections with various geometries and yield stresses that are carefully chosen to cover wide range of strength related parametric ratios like (i) distortional to local critical buckling stress ratio ($0.91{\leq}F_{CRD}/F_{CRL}{\leq}4.05$) (ii) non dimensional local slenderness ratio ($0.88{\leq}{\lambda}_L{\leq}3.54$) (iii) non-dimensional distortional slenderness ratio ($0.68{\leq}{\lambda}_D{\leq}3.23$) and (iv) yield to non-critical buckling stress ratio (0.45 to 10.4). The numerical investigation is carried out by conducting linear and non-linear shell finite element analysis (SFEA) using ABAQUS software. The non-linear SFEA includes both geometry and material non-linearity. The numerical results obtained are deeply analysed to understand the post buckling mechanics, failure modes and ultimate strength that are influenced by L-D interaction with respect to strength related parametric ratios. The ultimate strength data obtained from numerical analysis are compared with (i) the experimental tests data concerning L-D interaction mode buckling reported by other researchers (ii) column strength predicted by Direct Strength Method (DSM) column strength curves for local and distortional buckling specified in AISI S-100 (iii) strength predicted by available DSM based approaches that includes L-D interaction mode failure. The role of flange width to web depth ratio on post buckling strength erosion is reported. Then the paper concludes with merits and limitations of codified DSM and available DSM based approaches on accurate failure strength prediction.

• 한국통신학회논문지
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• 제27권7C호
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• pp.653-663
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• 2002

RSA 기반 서명 후 암호화 기법은 forward secrecy를 제공하는 반면 총 4번의 모듈러 멱승 연산을 요구하며, Zheng에 의해 제안된 signcryption은 논리적으로 한 번에 서명과 암호화를 수행하여 기존의 서명 후 암호화에서 요구되는 계산 비용보다 더 적은 비용을 가지는 반면 forward secrecy를 제공하지 못한다. 본 논문에서는 RSA 기반 서명 후 암호화 기법보다 적은 계산 비용과 통신 오버헤드를 가지며, 또한 forward secrecy를 제공하는 개선된 signcryption 기법을 제안한다. 제안 방안은 수신자의 비밀키를 사용하지 않고 직접 검증 가능한 방안으로 쉽게 변형될 수 있다. 또한 제안 signcryption 방안을 응용하여 공개키 사용 프로토콜의 선결과제인 CRL 처리 문제를 해결하면서 명시적인 송신 부인 방지 서비스를 제공하는 서버 지원 서명된(server-supported signature) 변형 signcryption 방안을 제시한다. 서버 지원된 변형 signcryption 방안은 기존의 signcryption 기법에도 적용이 가능하다.

• 한국임상수의학회지
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• 제25권6호
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• pp.501-505
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• 2008

Serial ultrasonographic examinations were performed on 9 Miniature Schnauzer bitches from day 15 of gestation until parturition to determine the ultrasonographic measurement of gestational structures. Ovulation was designated the day that plasma progesterone concentration exceeded 4.0 ng/ml (day 0). Extra-fetal structures were measured from day 17 or 18 to 60. Outer uterine diameter (OUD) increased from $6.50{\pm}1.06mm$ ($Mean{\pm}SD$) to $50.89{\pm}5.62mm$, inner chorionic cavity diameter (ICCD) increased from $2.10{\pm}0.15mm$ to $37.15{\pm}4.36mm$, and length of placenta (PL) increased from $7.50 {\pm}1.41mm$ to $40.62{\pm}3.27mm$. OUD and ICCD were significantly and linearly relative to gestational age especially through day 37, whereas PL was not significantly relative to gestational age. Of the extra-fetal structures, ICCD was the most accurate for estimation of gestational age before day 38 of gestation. Fetal structures increased at a linear rate to parturition. Fetal crown-rump length (CRL) increased from $2.55{\pm}0.07mm$ on day 22 to $85.25{\pm}9.89mm$ on day 48. Fetal head diameter (HD) increased from $3.43{\pm}0.64mm$ on day 27 to $25.06{\pm}0.41mm$ on day 63. Fetal body diameter (BD) increased from $ 5.96{\pm}0.84mm$ on day 30 to $43.76{\pm}3.36mm$ on day 63. Of the fetal structures, HD was the most accurate for estimation of gestational age after day 38 to parturition.

• Biomolecules & Therapeutics
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• 제29권2호
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• pp.195-204
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• 2021

Cereblon (CRBN), a substrate receptor of cullin 4-RING E3 ligase (CRL4) regulates the ubiquitination and degradation of c-Jun, mediating the lipopolysaccharide-induced cellular response. However, the upstream signaling pathway that regulates this process is unknown. In this study, we describe how endoplasmic reticulum (ER) stress reversely regulates sequestosome-1 (p62)and c-Jun protein levels. Furthermore, our study reveals that expression of p62 attenuates c-Jun protein levels through the ubiquitinproteasome system. Conversely, siRNA knockdown of p62 elevates c-Jun protein levels. Immunoprecipitation and immunoblotting experiments demonstrate that p62 interacts with c-Jun and CRBN to form a ternary protein complex. Moreover, we find that CRBN knockdown completely abolishes the inhibitory effect of p62 on c-Jun. Using brefeldin A as an inducer of ER stress, we demonstrate that the p62/c-Jun axis participates in the regulation of ER stress-induced apoptosis, and that CRBN is required for this regulation. In summary, we have identified an upstream signaling pathway, which regulates p62-mediated c-Jun degradation. Our findings elucidate the underlying molecular mechanism by which p62/c-Jun axis regulates the ER stress-induced apoptosis, and provide a new molecular connection between ER stress and apoptosis.