• Title/Summary/Keyword: CPD 분석

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Establishment of Genetic Characteristics and Individual Identification System Using Microsatellite Loci in Jeju Native Horse (초위성체 DNA를 이용한 제주마 집단의 품종특성 및 개체식별 체계설정)

  • Cho, Byung-Wook;Jung, Ji-Hye;Kim, Sang-Wook;Kim, Heui-Soo;Lee, Hak-Kyo;Cho, Gil-Jae;Song, Ki-Duk
    • Journal of Life Science
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    • v.17 no.10
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    • pp.1441-1446
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    • 2007
  • This study was conducted to establish the individual identification system and to estimate the genetic characteristic of Jeju native horse (JNH) using 13 microsatellite markers located on different horse autosomes. The markers were genotyped on 191 animals from five horse breeds including Jeju native horse (JNH). In total, 138 alleles were detected from the genotypes of 13 microsatellite markers. The average heterozygosities ranged from 0.317 to 0.902 and the polymorphic information content (PIC) ranged from 0.498 to 0.799 in JNH. We found that there were significant differences in allele frequencies in JNH when compared with other horse breeds. In ATH4 marker, there were specific allele frequence pattern that some of allele only found in JNH, Mongolian horse (MONG) and Jeju racing horse (JRH). The calculated cumulative power of discrimination (CPD) was 99.9% when nine microsatellite loci were used for analysis in the individual identification system. Also, the matching probability that two unrelated animals would show the same genotypes, was estimated to be $0.60{\times}10^{-10}$. Therefore, in the nine markers used in this study can be used for individual identification in the Jeju native horse population.

The Development and Validation of a Children's Play Disposition Scale (아동 놀이성향척도 개발 및 타당화 연구)

  • Sung, Jihyun;Byun, Hye-weon;Nam, Ji-hae
    • The Journal of the Korea Contents Association
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    • v.17 no.4
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    • pp.606-620
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    • 2017
  • The purpose of this study was to develop and validate a Children's Play Disposition Scale(CPDS) which could be used to evaluate children's play patterns and preferences. The participants of this study were parents of 437 5-7-year-old children (age range from 51months to 106months). Preliminary items were developed through a review of relevant research, multiple intelligence theory and scales, confirmation of item adequacy and content validity. After the content validity was confirmed by experts, these items were edited down to a final list of 27 items representing 6 factors identified by exploratory factor analysis. The 6 factors of the scale consists of initiative, linguistic activity, logical-mathematical activity, art and craft, physical activity, and sensitivity respectively. Concurrent validity was established by using correlations between each factor of the CPDS and sub-factors and the total scores of Multiple Intelligence Checklist for preschoolers (Multiple Intelligence Institute Co., Ltd, 2008) and Multiple Intelligence Checklist for elementary schoolers (Multiple Intelligence Institute Co., Ltd, 2007). In addition, the reliability of each factor, as measured by Cronbach's ${\alpha}$, ranged from .53 to .79. The CPDS provides the developmental and educational information for strengthening children's developmental forte and for supporting children's developmental weakness. This scale can be used on developing children's play contents and guiding play methods in the future.

Characterization of a Korean Traditional Porcine Breed Using Microsatellite Markers and the Establishment of an Individual Identification System (Microsatellite Marker를 이용한 한국재래돼지 집단의 품종특성 및 원산지 추적을 위한 개체식별체계 설정)

  • Kim, M.J.;Li, G.H.;Oh, J.D.;Cho, K.H.;Jeon, G.J.;Choi, B.H.;Lee, J.H.;Hong, Y.S.;Kong, H.S.;Lee, H.K.
    • Food Science of Animal Resources
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    • v.27 no.2
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    • pp.150-156
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    • 2007
  • This study was conducted to analyze the genetic characteristics of Korean Native Pigs(KNP), and to establish an individual identification system comprising many microsatellite markers located on different pig autosomes. Genotype data from 13 microsatellites typed in 446 animals was used to determine the validation of a method of individual identification in 4 KNP. A total of 112 alleles of the 13 microsatellites were detected and average heterozygosities(polymorphic information content) ranged from 0.286(0.423) to 0.686(0.796) in this study. Comparing the pattern of allele frequency among the KNP, Yorkshire, Landrace and Duroc breeds, there was specific differentiation between populations at multi-allelic loci. The cumulative power of discrimination(CPD) was 99.999% by including 10 microsatellite loci for the individual identification system. The probability that two different individuals incidentally have same genotype was estimated to be $0.36{\times}10^{-9}$. The system employing these 10 markers therefore proved to be applicable to the individual identification of KNP.