• 셀메드
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• 제13권14호
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• pp.15.1-15.15
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• 2023

Background and objective: A new formular CPC22 consists of Cynanchum wilfordii root, Pueraria thomsonii flower, and Citrus unshiu peel and has been developed to improve the postmenopausal symptoms. The research intended to evaluate whether CPC22 would regulate bone loss, hot flashes, and dysregulated lipid metabolism in ovariectomized (OVX) postmenopausal mice. Method: The OVX mice were orally administered with CPC22 daily for 7 weeks. Results: CPC22 regulated OVX-induced bon loss by enhancing serum osteoprotegerin, alkaline phosphatase, and osteocalcin levels and diminishing serum receptor-activator of the NF-κB ligand (RANKL), collagen type 1 cross-linked N-telopeptide, and tartrate-resistant acid phosphatase levels. As a result of CPC22 treatment, notable decreases in tail skin temperature and rectal temperature were observed, along with diminishment in hypothalamic RANKL and monoamine oxidase A levels and enhancement in hypothalamic serotonin (5-HT), norepinephrine, dopamine, 5-HT2A, and estrogen receptor-β levels. CPC22 enhanced levels of serum estrogen and diminished levels of serum follicle-stimulating hormone and luteinizing hormone. CPC22 regulated levels of serum lipid metabolites, including total cholesterol, triglycerides, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol. Furthermore, CPC22 diminished levels of serum blood urea nitrogen, creatine kinase, alanine transaminase, aspartate aminotransferase, and lactate dehydrogenase and restored vaginal dryness without affecting uterus atrophy index and vagina weights. Conclusion: Therefore, these results indicated that CPC22 improves OVX-induced bone loss, hot flashes, and dysregulated lipid metabolism by compensating for estrogen deficiency without side effects, suggesting that CPC22 may be used for the prevention and treatment of post menopause.

• 여성건강간호학회지
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• 제19권1호
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• pp.13-22
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• 2013

Purpose: This study was conducted to evaluate Korean version of the beliefs about Papanicolau (Pap) test and cervical cancer [CPC-28] in unmarried university students. Methods: The Korean version of CPC-28 (K-CPC-28) was developed through forward-backward translation techniques. A reliability, confirmatory factor analysis and correlations coefficients were evaluated. Data were collected from 303 unmarried female students attending university using a questionnaire that included28 items of CPC, 5 items of susceptibility of cervicalcancer and 8 items of HPV knowledge. Results: K-CPC-28 had reliable internal consistency with Cronbach's ${\alpha}$=.74 of six subscales ranged from .66 to .80. Factor loadings of the 28 items of subscales ranged from .31 to .86. Six factors in this study explained 55% of the total variance. In convergent validity of the K-CPC-28, the subscales of K-CPC-28 were significantly correlated with susceptibility scale of cervical cancer and HPV knowledge scale. Conclusion: K-CPC-28has satisfactory construct validity and reliability. It seems to be an acceptable tool to assess the attitudes toward cervical cancer prevention and Pap smear in unmarried women. This tool would be also applicable to screen the risk group in cervical cancer prevention and to identify its association with actual Pap test or cervical cancer prevention behaviors.

• Fisheries and Aquatic Sciences
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• 제19권4호
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• pp.22.1-22.7
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• 2016

One of the main compounds in Ishige okamurae, diphlorethohydroxycarmalol (DPHC), is known to exhibit antiviral and anti-inflammatory effects. However, it has not been investigated extensively. In this study, preparative centrifugal partition chromatography (CPC) coupled with 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) ($ABTS^+$) online HPLC was employed for effectively separating considerable amounts of antioxidant compounds from marine algae. Two main antioxidant compounds, DPHC and octaphlorethol A (OPA), respectively, were confirmed and isolated from the ethyl acetate (EtOAc) fraction of I. okamurae by $ABTS^+$ online HPLC and preparative CPC systems. The presence of DPHC and OPA was confirmed in the EtOAc fraction of I. okamurae by both liquid chromatography with diode array detection and electrospray ionization mass spectrometry (LC-DAD-ESI/MS) and $ABTS^+$ online HPLC systems: DPHC (39 mg) and OPA (23 mg) were successfully isolated from I. okamurae (500 mg) with optimum solvent composition (0.5:10:4:6; n-hexane/EtOAc/MeOH/water, v/v) with corresponding partition coefficients (K) of 1.62 and 2.71, respectively, by preparative CPC. Hence, CPC coupled with $ABTS^+$ online HPLC is convenient for the efficient and simple isolation of these antioxidant compounds from I. okamurae.

• 한국미생물·생명공학회지
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• 제22권3호
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• pp.296-303
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• 1994

An immobilized Trigonopsis variabilis cells having an high activity of D-amino acid oxidase(DAO) was used to convert CPC into GL-7-ACA. The optimal pH of the reaction system was 8.0-8.5, and the optimal temperature was 40$\circ$C. When immobilized cell was used repeatedly in semi-batchwise reaction, the system retained 80% of the initial activity after used of 12 times for over 12 hours. The storage stability of the immobilized cell was maintained for 30 days at 4$\circ$C. The CPC concentration for the maximal reaction rate was about 30 mM and 40 mM for free and immobilized cells, respectively. Substrate inhibition of CPC concentration more than 50 mM was overcomed by 20~25% by immobilization. Pure oxygen supply into reaction system was most efficient in D-amino acid oxidase reaction. Continuous conversion to GL-7-ACA from CPC has been developed with an bioreactor system containing immobilized T variabilis cells. By opera- tion of the reactor for 5 hours, the average conversion yield of >80% and GL-7-ACA production of 40~45 mM per hour could be obtained.

• Asian Pacific Journal of Cancer Prevention
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• 제16권2호
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• pp.601-606
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• 2015

Background: Prostate cancer is predominately a disease of older men, with a median age of diagnosis of 68 years and 71% of cancer deaths occurring in those over 75 years of age. While prostate cancer screening is not recommended for men >70 years, fit elderly men with controlled comorbidities may have a relatively long life expectancy. We compare the use of age related PSA with the detection of primary malignant circulating prostate cells mCPCs to detect clinically significant PC in this population. Materials and Methods: All men undergoing PC screening with a PSA >4.0ng/ml underwent TRUS 12 core prostate biopsy (PB). Age, PSA, PB results defined as cancer/no-cancer, Gleason, number of positive cores and percentage infiltration were registered. Men had an 8ml blood sample taken for mCPC detection; mononuclear cells were obtained using differential gel centrifugation and mCPCs were identified using immunocytochemistry with anti-PSA and anti-P504S. A mCPC was defined as a cell expressing PSA and P504S; a positive test as at least one mCPC detected/sample. Diagnostic yields for subgroups were calculated and the number of avoided PBs registered. Esptein criteria were used to define small grade tumours. Results: A total of 610 men underwent PB, 398 of whom were aged <70yrs. Men over 70 yrs had: a higher median PSA, 6.24ng/ml versus 5.59ng/ml (p=0.04); and a higher frequency of cancer detected 90/212 (43%) versus 134/398 (34%) (p=0.032). Some 34/134 cancers in men <70yrs versus 22/90 (24%) of men >70yrs complied with criteria for active surveillance. CPC detection: 154/398 (39%) men <70yrs were CPC (+), specificity for cancer 86%, sensitivity 88%, 14/16 with a false (-) result had a small low grade PC. In men >70 years, 88/212 (42%) were CPC (+); specificity 92%, sensitivity 87%, 10/12 with a false (-) had small low grade tumours. False (+) results were more common in younger men 36/154 versus 10/88 (p<0.02). With a PSA cutoff of 6.5ng/ml, in men <70yrs, 108 PB would be avoided, missing 56 cancers of which 48 were clinically significant. Using CPC detection, 124 biopsies would be avoided, missing only 2 clinically significant cancers. In men >70 yrs using a PSA >6.5ng/ml would have resulted in 108 PB with 34 PC detected, of which 14(41%) were small low grade tumours. Conclusions: The use of CPC detection in the fit elderly significantly decreases the number of PBs without missing clinically significant cancers, indicating superiority to the use of age-related PSA.

• 한국입자에어로졸학회지
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• 제17권2호
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• pp.21-27
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• 2021

We developed non-metallic electrodes that can replace metallic electrodes of the electrostatic precipitator(ESP) for the purpose of light weight, corrosion resistance, cost reduction. We manufactured three types of collection electrodes made of stainless steel (M), Carbon ink coating layer-Plastic sheet-Carbon ink coating layer (CPC), and Plastic sheet-Carbon ink coating layer-Plastic sheet (PCP). We studied the collection efficiency of a two-stage ESP using oil mist particles with and without collection stage by changing the flow rate, the material of collection electrode, and the applied voltage of the pre-charger module and the collection module. Here we measured concentrations of particles at diameters of 0.45 ㎛ (CMD; count median diameter) and 3.0 ㎛ (MMD : mass median diameter), as well as PM_2.5 and PM₁₀. As a result of the experiment, two-stage ESP had 22~25% higher collection efficiency in PM_2.5 than one-stage ESP at the same applied voltage. The difference in collection efficiency by varying the materials of collection electrodes was less than 5%. The weight of the non-metallic electrode was only one eighth the weight of the metal electrode. CPC electrode had a thickness of 0.27 mm, which was 1.5 times thinner than a thickness of PCP electrode, so when the flow rate increased, the CPC electrodes couldn't be kept at equal intervals due to the fluttering unlike PCP electrodes. In addition, the PCP-CPC collection module of the present experiment followed the theoretical efficiency based on Deutsch equation and Cochet's charging theory.

• 한국자동차공학회논문집
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• 제15권3호
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• pp.153-159
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• 2007

Nano-Particles are influenced on the environmental protection and human health. The relationships between transient vehicle operation and nano-particle emissions are not well-known, especially for diesel passenger vehicles with DPF. In this study, a diesel passenger vehicle was measured on condition of DPF regeneration and no regeneration on a chassis dynamometer test bench. The particulate matter (PM) emission from this vehicle was measured by its number, size and mass measurement. The mass of the total PM was evaluated with the standard gravimetric measurement method while the total number and size concentrations were measured on a NEDC driving cycle using Condensation Particle Counter (CPC) and EEPS. Total number concentration by CPC was $1.5{\times}10^{1l}N/km$, which was 20% of result by EEPS. This means about 80% of total particle emission is consist of volatile and small-sized particles(<22nm). During regeneration, particle emission was $6.2{\times}10^{12}N/km$, was emitted 400 times compared with the emission before regeneration. As for the particle size of $22{\sim}100nm$ was emitted mainly, showing peak value of near 40nm in size. This means regeneration decreased the mean size of particles. Regarding regeneration, PM showed no change while the particle number showed about 6 times difference between before and after regeneration. It seems that the regeneration influences on particle number emissions are related to DPF-fill state and filtration efficiency.

• 한국미생물·생명공학회지
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• 제32권3호
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• pp.224-229
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• 2004

토양으로부터 강력한 CAH activity를 갖는 균주를 분리하여, 생화학적, 배양학적, 전자현미경 동정을 한 결과, Bacillus sphaericus로 확인되었으며, 이를 B. sphaericus 366M-9로 명명하였다. 또한 이 균으로부터 최초로 cephalosporin-C deacetylase(CAH)를 분리 정제하였다. 정제수율은 약 7.5% 였으며, B. sphaericus 366M-9에서 분리한 CAH-9의 최적활성 온도는 $50^{\circ}C$였으며, 효소안정 온도구간은 30～$50^{\circ}C$이다. 또한 최적 활성 pH는 7.0이었으며, 효소안정 pH구간은 pH 6.0～8.0으로 90% 이상의 잔존 활성도를 나타내었다. 효소반응속도에 미치는 기질의 영향에서는 $K_{m}$ 값은 0.87 mM 이며,$ V_{max}$는1.22 unit/ml이었다.다.

• 한국경영과학회지
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• 제38권1호
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• pp.123-138
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• 2013

Keyword advertising has been used as a promotion tool rather than the advertising itself to online retailers. This is because the online retailer expects the direct sales increase when they deploy the keyword sponsorship. In practice, many online sellers rely on keyword advertising to promote their sales in short term with limited budget. Most of the previous researches use direct revenue factors as dependent variables such as CTR (click through rate) and CVI (conversion per impression) in their researches on the keyword advertising[14, 16, 22, 25, 31, 32]. Previous studies were, however, conducted in the context of aggregate-level due to the limitations on the data availability. These researches cannot evaluate the performance of keyword advertising in the individual level. To overcome these limitations, our research focuses on conversion of keyword advertising in individual-level. Also, we consider manageable factors as independent variables in terms of online retailers (the costs of keyword by implementation methods and meanings of keyword). In our study we developed the keyword advertising conversion model in the individual-level. With our model, we can make some theoretical findings and managerial implications. Practically, in the case of a fixed cost plan, an increase of the number of clicks is revealed as an effective way. However, higher average CPC is not significantly effective in increasing probability of purchase conversion. When this type (fixed cost plan) of implementation could not generate a lot of clicks, it cannot significantly increase the probability of purchase choice. Theoretically, we consider the promotional attributes which influence consumer purchase behavior and conduct individuals-level research based on the actual data. Limitations and future direction of the study are discussed.

• 한국초지조사료학회지
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• 제35권1호
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• pp.50-56
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• 2015

이탈리안 라이그라스(Lolium multiflorum Lam., IRG)는 주로 답리작으로 재배되고 있으며 파종기는 벼 수확시기와 겹치고 파종기에 강우에 의하여 파종 적기를 놓칠 수 있다. 따라서 본 연구는 이탈리안 라이그라스의 적기 파종을 놓쳤을 때 봄 파종시 수량성 및 사료가치에 미치는 영향을 검토하고자 2012월 10월부터 2013년 6월까지 전라남도 강진에서 수행하였다. 파종시기를 주구로 2월 15일, 2월 25일, 3월 5일, 3월 15일로 4처리 하였으며, 품종을 세 구로 극조생종 그린팜(Greenfarm), 조생종 코윈어리(Kowinearly) 그리고 중생종 코윈마스터(Kiwinmaster)를 공시하였다. 이탈리안 라이그라스의 파종시기별 출수기는 2월 15일 파종에서는 Kowinmaster, Kowinearly 및 Greenfarm은 각각 5월 14일, 5월 12일, 5월 9일로 극조생종과 중생종간의 차이는 5일의 숙기 차이가 있었으나, 3월 15일 파종에서는 각각 5월 23일, 5월 22일, 5월 20일로 극조생종과 조생종간의 생육차이는 3일의 숙기 차이가 나타났다. 그리고 초장은 Kowinmaster를 2월 15일 파종하였을 때 105.7 cm로 3월 15일에 파종하였을 93.0 cm 보다 길었으며, Kowinearly와 Greenfarm 품종도 2월 15일과 2월 25일 파종구에서 초장은 비슷하였으나, 3월 5일과 3월 15일에서는 짧았다. 그리고 도복은 빨리 파종하였을 때 심했으며, 품종간의 차이는 적었다. 그리고 건물수량은 Kowinearly 조생종을 2월 15일에 파종하여 5월 30일에 수확할 때 7,333 kg/ha으로 가장 많았고, Green farm 극조생종에서 6,609 kg/ha으로 적었으며, 유의적 차이는 없었다. 그러나 파종시기별 건물수량은 2월 15일에 파종했을 때 7,052 kg/ha으로 3월 15일 파종구의 4,698 kg/ha 보다 유의적으로 많았다(P<0.05). 조단백질의 경우 2월 15일 파종에서 평균 8.8%인데 반해 3월 15일 파종에서 평균 11.1%로 약 2.3% 높게 나타났으며, ADF는 2.1% 낮게 나타났고 TDN도 1.5% 정도 높았다. 그리고 RFV에서는 5.9% 정도 높아 파종시기가 늦어짐에 따라 사료가치는 좋았으며 품종간의 차이는 적었다. 이상의 결과를 종합적으로 고려할 때 남부지역 논에서 이탈리안 라이그라스의 봄 파종 적기는 2월 25일경이며, 최소한 2월말까지는 파종해야 수량성과 사료가치가 높은 조사료(forage)를 생산할 수 있을 것으로 사료된다.