• Title/Summary/Keyword: CMVP0-CP

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Assessment of the Persistence of DNA in Decomposing Leaves of CMVP0-CP Transgenic Chili Pepper in the Field Conditions (포장 조건에서 CMVP0-CP 형질전환 고추 도입유전자의 지속성 조사)

  • Lee, Bum-Kyu;Kim, Chang-Gi;Park, Ji-Young;Park, Kee-Woong;Yi, Hoon-Bok;Harn, Chee-Hark;Kim, Hwan-Mook
    • Korean Journal of Environmental Agriculture
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    • v.26 no.4
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    • pp.319-324
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    • 2007
  • This study was conducted to evaluate the persistence of DNA in the transgenic chili pepper resistant to cucumber mosaic virus (CMV) in the field condition. We analyzed the persistence of genes in the leaf samples obtained from two field conditions, below and above soil. Transgenic and non-transgenic leaf tissues were buried in the soil at a depth of 10 cm or placed on the soil surface. Qualitative and quantitative PCR analysis showed that the amount of transferred genes from the transgenic peppers below and above soil was dropped to 28.3-42.7% one month after buried and it was rapidly reduced to 0.9-3.3% after two months. The transgenes were not detected three to four month after buried. In addition, DNA of the leaves placed below soil decomposed about 8%more than those on soil surface. The gene transfer from decomposing leaves of the transgenic pepper to soil was investigated by PCR analysis with the soil attached to the samples. The PCR result indicated that the gene transfer from the transgenic pepper to soil was not occurred.

The Comparative Study of Arthropods Community on Non-transgenic Mother Chili Pepper (P915) and Transgenic Chili Pepper (CMVP0-CP) in the Isolated Quarantine LMO Fields (LMO 격리 포장에서 유전자비변형 모본 고추(P915)와 유전자 변형 고추(CMVP0-CP)에 서식하는 절지동물 군집 비교연구)

  • Yi, Hoon-Bok;Kim, Hyun-Jung
    • Korean journal of applied entomology
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    • v.49 no.1
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    • pp.23-29
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    • 2010
  • The environmental risks of cucumber mosaic virus resistant transgenic chili peppers with the CMVP0-CP gene on non-target organisms in the agroecosystem environments was evaluated during the periods of the chili pepper growing season (June 19, July 30, August 31) in 2007. Arthropods assemblages leaves and flowers of chili peppers were quantitatively collected by using an insect vacuum collector to compare the arthropod community structures between non-transgenic chili peppers (nTR, P 915) and mosaic virus resistant transgenic chili peppers (TR, CMV-cp, line 7). There was no statistical difference in the arthropod community structure between the two types of crops, nTR and TR, at the same season, although the species richness and Shannon's index were somewhat different among seasons; indicating no effects of genetically modified peppers on the arthropod community. However, further studies were required to conclude more concretely for the potential environmental risk of the transgenic chili pepper of CMV-cp.