• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1998년도 Proceedings of UNESCO-internetwork Cooperative Regional Seminar and Workshop on Bioassay Guided Isolation of Bioactive Substances from Natural Products and Microbial Products
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• pp.178-178
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• 1998

We reported that the hexane fraction of Torilis Fructus have an anti-inflammatory and analgesic effect. Therefore, in order to isolate the active compound, the hexan fraction of Torilis Fructus was chromatographed on silica gel column. The subfraction of hexane fraction was crystallized as colorless stout needles. The chemical structure of this compound was verified to be torilin through m.p., UV, IR, GC-MS, and NMR spectral data. In pharmacological tests, torilin exhibited strong anticarrageenan activity at the dose of 90 and 270 mg/kg, p.o. in rats, and it had inhibitory effect on the vascular permeability at the dose of 30 and 90 mg/kg, p.o. in mice. Torilin showed potent inhibition of leucocyte emigration in CMC-pouch at the dose of 3 and 9 mg/rat, s.c. Torilin have the analgesic effect at the dose of 30, 90 and 270 mg/kg, p.o. in both of the acetic acid- and phenyl-p-benzoquinone-induced writhing syndrome. It also increased the pain threshold at the dose of 30, 90 and 270 mg/kg, p.o. in the tail pressure method and the Randall-Selitto method. Torilin did not show a hypothermic action at the dose of 30 and 90 mg/kg, p.o. in mice. The acute toxicity of torilin was very weak: the LD$\_$50/ value was more than 5000 mg/kg, p.o. and 2000 mg/kg, Lp. in mice. From the above mentioned results, it was suggested that torilin had potent anti-inflammatory and analgesic activities in animals.

• 미생물학회지
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• 제14권2호
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• pp.65-74
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• 1976

Atternaria sp. was isolated from soil and crude cellulases were prepared from wheat bran culture of the fungus. The activities of the crude enzyme were studied on five different subvstrates and some phsical properties were also examined, crude enzymes were purified by column chromatography on DEAE Sephadex and Sephadex, Isozymes were separated some of which were active specifically on DEAE cellulose and some were primarily active on cellulose and CM-cellulose. The optimal points of pH and temperature for the crude enzyme were varied depending on the substrates ; On cellulose they were at pH 6.0 and 40.deg.C, on CM-cellulose at pH's 4.0 and 6.0 and 60.deg.C, and on DEAW-cellulose at pH 5.0 and 50.deg.C. Two active fractions, F-1 and F-II on Na-CMC was used as substrate the Km values of crude enzyme, F-I and F-II were calculated to be $4{\times}10^{-5}$ , 1.1 * 10$^{-4}$ , and $1.25{\times}10^{-4}mN$ resepctively. The Ki value of $Cu^{++}$ for crude enzyme was$4{\times}^{-4}mN$ , while that of $Nm^{++}$ while in the same concentration of $Mn^{++}$ it reached to 91%. Some 57% activity of F-1 was inhibited in s mN $Cu^{++}$, whereas it was inhibited as much as 81% in the same concentration above the concentration of 0.3 mM with tis activity reaching up to 137% in 2 mM. On the other hand the F-11 was inhibited by the presence of M $n^{++}$ and some 67% activity was inhibited at 2mM.

• 미생물학회지
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• 제36권1호
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• pp.20-25
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• 2000

Trichoderma sp. C-4의 배양액으로부터 한 종류의 endoglucanase(F-II-II)를 Sephacryl S-200 및 Sephacryl S-100 chromatography를 통하여 분리하였다. 분리된 효소는 SDS-PAGE및 isoelectric focusing을 통하여 단일 band로 나타났으며, 분자량이 26,000, 등전점이 8.0으로 나타났다. 이 효소의 반응 최적온도와 최적 pH는 각각 $50^{\circ}C$, 5.0 이었으며, $50^{\circ}C$에서 24시간 동안 안정하였다. 분리된 효소의 carboxymethylcellulose에 대한 specific activity는 776.2 U/mg protein으로 나타났다. 이 효소의 아미노산 조성을 조사하였다. 효소를 trypsin으로 가수분해한 후 분해산물에 대한 단백질서열 분석을 행하였다. 그 결과 이 효소의 서열은 현재까지 밝혀진 다른 단백질과 homology를 갖지 않음이 확인되었다. 이 효소는 그 specific activity가 대단히 높고 아직 보고되지 않은 novel protein일 가능성이 대단히 높기 때문에 좋은 유전자 자원이 될 것으로 사료되었다.

• 자원환경지질
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• 제35권5호
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• pp.421-428
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• 2002

계면활성제를 사용하여 지하수의 표면장력을 인위적으로 감소하였을 때, 감소된 표면장력이 지하수 폭기시의 폭기 영향권 및 VOC 제거 효율에 미치는 영향을 실험실 규모의 실험을 통하여 규명하였다. 내경 9.5 cm, 길이 100 cm의 유리 컬럼에 계면활성제(sodium dodecyl sulfate, SDS) 및 toluene 100 ppm 포함하는 물로 채운 후 일정한 속도로 공기를 주입하였을 때의 toluene의 제거 속도를 측정하였다. 또한 같은 컬럼에 토양(모래)이 채워진 상태에서 동일한 실험을 반복하였다. 토양이 존재하지 않는 상태에서의 stripping에 의한 toluene의 제거 속도는 표면장력의 감소(계면 활성제 농도의 증가)에 따라 증가하는 것으로 나타났으며, 토양이 존재하는 경우에도 비슷한 결과를 나타내었다. 2차원 유리 상자에 모래와 SBS를 포함하는 물을 채운 후 일정한 공기 유속을 유지하였을 때, 폭기의 영향권(공기의 침투영역)은 물의 표면장력 감소에 따라 현저히 증가하는 것으로 나타났다. 특히 SDS의 critical micelle concentration(CMC)보다 훨씬 낮은 농도에서 폭기 영향권이 최대화하는 것으로 밝혀졌다. 본 연구결과는 폭기에 의한 지하수 오염물질 중, 특히 휘발성 유기오염물질의 제거 공정의 효율을 증대하는데 기여할 것으로 생각된다.

• 공업화학
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• 제5권2호
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• pp.247-254
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• 1994

디글리세롤 N-아실 글루탐산 에스테르 비이온성 계면활성제를 4급 암모늄염을 촉매로 사용하여 글리시돌과 N-아실글루탐산과 에스테르 반응시켜 합성하였다. 표면장력법에 의해서 산정한 임계미셀농도는 $0.15{\sim}0.75mol/{\ell}$이었고 수용액에서의 표면장력은 28~35dyne/cm로 저하되었다. 또한 아실기의 탄소수가 12인 L-DGLG가 가장 좋은 기포력과 유화력을 나타냈고, 대두유보다 톨루엔에서 유화력이 높게 관찰되었다. 카본블랙과 산화철에서 아실기 탄소수 16과 18인 L-DGPG와 L-DGSG는 L-DGLG보다 분산성이 우수했으며, 이들은 모두 좋은 생분해성을 나타냈다.

• Journal of Microbiology and Biotechnology
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• 제15권2호
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• pp.302-309
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• 2005

Phytopathogenic Pectobacterium carotovorum subsp. carotovorum (Pcc) LY34 secretes multiple isozymes of the plant cell wall degrading enzyme endoglucanases. We have cloned a third cel gene encoding CMCase from Pcc LY34. The structural organization of the celC gene (AY188753) consisted of an open reading frame (ORP) of 1,116 bp encoding 371 amino acid residues with a signal peptide of 22 amino acids within the NH$_2$-terminal region of pre-CelC. The predicted amino acid sequence of CelC was similar to that of Peetobaeterium ehrysanthemi Cel8Y (AF282321). The CelC has the conserved region of the glycoside hydrolase family 8. The apparent molecular mass of CelC was calculated to be 39 kDa by CMC-SDS-PAGE. The cellulase­minus mutant of Pee LY34 was as virulent as the wild-type in pathogenicity tests on tubers of potato. The results suggest that the CelC of Pce LY34 is a minor factor for the pathogenesis of soft-rot.

• 한국염색가공학회지
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• 제19권6호
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• pp.21-27
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• 2007

The factors which affect the absorption and diffusion of disperse dye in print paste for polyester film were investigated using film roll method. When Emvatex print paste of different disperse dye concentrations were fixed by high temperature steaming(HTS) for 2 hr at $190^{\circ}C$, the dye uptake and diffusion distance of 50g/kg dye concentration for PET film were very low, but those of 100$\sim$300g/kg were increased with increasing dye concentration. It was found that sodium alginate(5%) was more effective for the absorption and diffusion of disperse dye to PET film than CMC(3%) and Emvatex(18%), and Emvatex showed comparatively low absorption in three thickeners used. The dye uptake and diffusion distance of disperse dye in sodium alginate paste for PET film were increased with increasing paste thickness to $160\sim180{\mu}m$, but were decreased at $200\sim220{\mu}m$ in both fixations of baking and HTS for 2 hr at $190^{\circ}C$, and were far more largely increased by fixation of HTS than baking because steam was very important for fixation. Also it was confirmed that dye uptake was slightly increased as steam supply pressure was raised from 1 $kg/cm^2$ to 2 $kg/cm^2$, andthe dye uptake and diffusion distance of disperse dye were outstandingly increased with raising fixing temperature from $170^{\circ}C$ to $190^{\circ}C$ and fixing time from 0.5 hr to 3 hr in the fixation of HTS.

• 대한후두음성언어의학회지
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• 제19권2호
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• pp.117-122
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• 2008

Background and Objectives: $Radiesse^{(R)}$ is a gell-formed material of calcium hydroxylapatite (CaHA) and carboxymethylcellulose (CMC) used for vocal fold injections. The authors aimed to study injection laryngoplasty with $Radiesse^{(R)}$, and determine the efficacy of $Radiesse^{(R)}$ for unilateral vocal cord palsy using objective and subjective measures. Materials and Method: Nine patients with unilateral vocal cord palsy received injection laryngoplasty with $Radiesse^{(R)}$ under general anesthesia from Jul. 2007 to Jan. 2008. $Radiesse^{(R)}$ was injected with 25gauze long needle perorally or percutaneously. The Acoustic, aerodynamic, stroboscopic analysis and pre-injection/post-injection perceptual assessment were evaluated in all patients. Results: Postoperative jitter and shimmer scores tended to diminish compared to preoperative scores, and maximum phonation time increased with statistical significantly. Stroboscopic findings demonstrated improvement postoperatively. The degree of hoarseness, which is a part of perceptual assessment, decreased after the procedure. Conclusion: $Radiesse^{(R)}$ may be an altemative material for injection laryngoplasty. We suggest long-term follow up with more cases.

• 생명과학회지
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• 제14권2호
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• pp.320-324
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• 2004

본 연구는 biosurfactant를 생산하는 우수한 균주를 얻고자 산지 토양에서 생산능이 우수한 균주 수십 종을 분리하였다. 분리된 균주 중 원유분해능 및 biosurfactant 생성능이 우수한 균주를 선별하여, 형태학적 특성 및 생리 화학적 특성을 조사고, 16S rDNA의 부분적 염기서열 결정을 통하여 Bacillus sp. TBM911-5로 동정하였다. 동정된 균주 배양액에 생산된 biosurfactant에 의해 48시산 정도에서 표면장력이 최저 29mN/m까지 감소되었다. Bacillus sp. TBM 911-5가 생산하는 biosurfactant의 유화활성은 대두유를 기질로 사용하였을 때 최대였으며, 원유에서도 높은 편이였다. 유화안정성은 합성 계면활성제의 Tween 류나 Triton X-100등과 비슷하거나 우수하였으며, 이는 분리된 균이 생산하는 biosurfactant의 계면활성제로서의 산업적 이용 가능성을 보여준다.

• KSBB Journal
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• 제22권4호
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• pp.213-217
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• 2007

첨가제가 단백질 약물 방출 속도 및 약물 제제 제조 및 구조에 미치는 영향을 고찰하였다. 친수성 첨가제인 D-sorbitol의 경우 친유성 첨가제보다 단백질 약물 방출 속도를 감소시킬 수 있었으며 최적의 농도는 3% (w/v)로 나타났다. 또한 제제 제조시 점도를 낮게 유지할 뿐 아니라 상분리 없는 균일한 pluronic 용액상태를 유지하여 약물이 첨가될 경우에 균일한 약물제제를 만들 수 있었다. 한편 D-sorbitol은 pluronic 수용액의 CMC를 낮추고 마이셀 표면에 작용하여 구조를 강화하는 역할을 수행하는 것으로 보인다. 따라서 pluronic 제제에 D-sorbitol을 첨가하여 단백질 약물의 안정성을 향상시키고 효과적인 약물전달 시스템을 설계할 수 있었다.