• Korean Journal of Oriental Medicine
• /
• v.15 no.3
• /
• pp.75-82
• /
• 2009

Anti-mutagenic and anti-septic effects of $\beta$-1,3/1,6-glucan from Aureobasidium pullulans SM-2001 were evaluated on the on the cyclophosphamide (CPA)-cecal ligation puncture (CLP) and CPA-treated mice. To induce immunosuppression and mutagenicity, 150 and 110 mg/kg of CPA were single intraperitoneally injected at 3 or 1 day before CLP or initial $\beta$-glucan administration. In CLP animals, the cecum was mobilized and ligated below the ileocecal valve, punctured through both surfaces twice with a 22-gauge needle. 125 mg/kg of $\beta$-glucan were dissolved in saline and subcutaneously or orally administered in a volume of 10 ml/kg (of body weight), 4 times, 12 hrs intervals from 6 hrs after CLP or 1 day after second dose of CPA. After treatment of $\beta$-glucan, the mortalities were observed in CPA-CLP model, and the appearance of a micronucleus is used as an index for genotoxic potential in CPA model. As results of CPA-CLP sepsis, all animals (9/9, 100%) in CPA-CLP control were dead within 2 days after CLP. In addition, increase of the number of bone marrow MNPCEs indicated mutagenicity were also observed by treatment of CPA. However, $\beta$-glucan treatment effectively inhibited the mortalities in CPA-CLP, and it also reduced the CPA treatment-related mutagenicity, respectively. These results indicated that $\beta$-glucan has effective anti-septic and anti-mutagenic effects and can be used as an agents for treating sepsis and mutagenicity related to high-dose chemotherapy or radiotherapy. However, further studies should be conducted to observe more detail action mechanisms of it's anti-septic and anti-mutagenic effects.

• Journal of the East Asian Society of Dietary Life
• /
• v.21 no.4
• /
• pp.535-544
• /
• 2011

The purpose of this study was to investigate the quality characteristics and antioxidative effect of cookies prepared with Curcuma longa L. powder (0, 1, 2, 3, 4%) substituted for flour. The pH of cookie dough decreased significantly in response to the addition of all levels of Curcuma longa L. powder (CLP). However, there were no significant differences in dough values among the test groups. Furthermore, when the spread factor values were compared among the groups, they were found to be inversely proportional to the CLP concentration. In addition, Hunter's color L value significantly decreased with increasing CLP content, whereas the a and b values increased. According to textural measurements, substitution of 1~4% for CLP resulted in increased hardness, cohesiveness, springiness, and brittleness when the samples were compared to the control. The results of sensory evaluation showed that the cookies containing 2% CLP had the highest scores. The acid value, peroxide value, and thiobarbituric acid value were all lower in cookies with 2% and 3% CLP than in cookies made with 4% CLP and control cookies.

• KSBB Journal
• /
• v.26 no.1
• /
• pp.62-68
• /
• 2011

To isolate Lactic acid bacteria for animals, we have screened from Kim-chi, swine intestine, swine feces, and dairy products by random selection and anti-viral, antipathogenic bacteria test. Among them, CLP-1 shown that inhibitory effect against rotavirus, porcine epidemic diarrhea (PED) virus, Salmonella sp, and E.coli. By examining biological property, API-ZYM and identified Lactobacillus plantarum by 16S rDNAgene sequence. CLP-1 determined resistance to low pH and bile salt. Futhermore, the cell body of CLP-1 adhered to the intestinal epithelium tissue of swine and Caco-2 cell. CLP-1 was examined on cell immune system modulating activity in vitro. The whole cell and cell culture supernatant was increasing of interferon-${\beta}$ activity. And then, CLP-1 increased prevention effect by Salmonella enteritidis infection in SPF chickens. And we determined similar result in pigs.

• Proceedings of the Korean Society of Applied Pharmacology
• /
• 2001.11a
• /
• pp.109-109
• /
• 2001

The present study was done to investigate the relationship between Kupffer cells and alteration of cytochrome P-450 (CYP)-dependent drug metabolizing enzyme activities during polymicrobial sepsis. Male rats were subjected to polymicrobial sepsis by cecal ligation and puncture (CLP) followed by fluid resuscitation. The gadolinium chloride (GdC1$_3$, 10 mg/kg), blocker of Kupffer cells, was pretreated intravenously at 48 h and 24 h prior to the induction of CLP. All assay parameters were determined at 24 h after CLP or sham operation. In CLP-treated rats, the mortality rate of animals increased to 50% and serum alanine (ALT) and aspartate aminotransferase (AST) levels also significantly elevated. However, this increase was not suppressed by GdC1$_3$ pretreatment. Microsomal lipid peroxidation markedly increased after CLP operation. This increase was significantly attenuated by pretreatment. Total cytochrome P-450 content and NADPH-cytochrome P-450 reductase activity were not changed after CLP operation, but GdC1$_3$pretreatment reduced total cytochrome P-450 content, The hepatic microsomal CYP 1A1, 1A2, 2Bl and 2El activities in CLP-induced rats were also not significantly different from sham-operated rats. However, GdC1$_3$pretreatment showed a moderate increase in CYP1A1 and 1A2 activities. Our findings suggest that Kupffer cells may be partly responsible for producing hepatocellular dysfunction during sepsis.

• Journal of Korean Academy of Nursing
• /
• v.41 no.5
• /
• pp.581-592
• /
• 2011

Purpose: The purpose of this study was to identify and describe nurses' experience of a career ladder program (CLP) in a general hospital in Korea. Methods: The data were collected from 15 nurses who participated in the CLP. Each nurse completed individual in-depth interviews, for which the main interview question was "How do you describe your experience of CLP?" All interview data were transcribed and were analyzed using the grounded theory method. Results: The core category of the experience of CLP was identified as "challenging to rebirth as an expert". The participants used five action/interaction strategies such as "taking some time for introspection", "appreciating the essence of nursing", "making use of feedback positively", "throwing away doubts about their own capabilities", "exerting themselves with confidence". The consequence of the process of adjusting to CLP was "positioning a differentiated expert". Conclusion: The results of this study show that participants recognized CLP as a powerful resource which leads them to professional growth and development. Thus, CLP could be utilized as a clinical career-promoting program and ultimately as a program for providing high-quality nursing services.

• Asian-Australasian Journal of Animal Sciences
• /
• v.22 no.9
• /
• pp.1296-1302
• /
• 2009

Ninety six beak-trimmed 72 week-old Lohmann Brown hens were randomly divided into four equal groups. Each group comprised 4 replicates. Isoenergetic and isonitrogenous experimental diets contained low calcium (3.5%); optimum calcium (4.2%); low Ca (3.5% Ca)+1% Clinoptilolite (CLP); low Ca (3.5% Ca)+2% CLP. Data were collected biweekly and the experiment lasted 6 weeks. Egg production, feed consumption, feed conversion ratio, egg weight, tibia Ca, P, ash and eggshell thickness were not affected by addition of CLP to the diets (p>0.05). There were no significant differences in egg shell strength and ash when data were analyzed individually in measurement periods ($74^{th}$, $76^{th}$ and $78^{th}$ weeks). However, according to pooled data ($74^{th}$-$78^{th}$ weeks), eggshell strength was increased (p<0.05) only by 2% CLP supplementation versus low Ca (3.5%) diet, and shell ash was significantly increased by 2% CLP supplementation compared with the other diets. The damaged egg ratio on 1% and 2% CLP diets was significantly decreased between 76-78 weeks'data when compared with the low Ca diet. However; damaged egg ratio on the 2% CLP diet was significantly decreased when pooled data (74-78) were compared with no CLP diets. The differences in marketable egg ratio paralleled damaged egg ratio. The plasma calcium level at the end of experiment was increased on the 2% CLP diet when compared with the low Ca (3.5%) diet (p<0.05). Furthermore, at the end of the experiment a marked decrease of manure moisture was observed on both CLP diets (p<0.01). In conclusion, Clinoptilolite (2%) supplementation to layer diets tends to improve eggshell quality and manure dry matter (1% and 2% CLP) after six weeks.

• Korean Journal of Cleft Lip And Palate
• /
• v.5 no.2
• /
• pp.95-108
• /
• 2002

언어 발달의 조기 단계를 이해하기 위한 일환으로 crying은 언어전 발달의 기초 단계로서 여러 학문적 분야에서 많은 연구가 있어왔다. 그러나 구순구개열(CLP))환아의 경우는cry-producing/control mechnism에 variation이 많은 이유로 이 분야의 연구는 거의 없는 실정이다. 이에 본 연구에서는 다음과 같은 의문점을 가지고 CLP환아의 cry feature에 대한분석을 하였다. 첫째, 정상아와 CLP환아의 cry에 전형적인 차이가 있는가? 둘째, CLP환아의 술전, 술후 cry feature에 변화가 있는가? 셋째, cry분석이 CLP환아의 이후 speech disorder에 대한 언어전 평가로서의 가치가 있는가? 넷째, 특정 parameter가 언어전 평가에 적절한 도구로 작용할 수 있는가? 생후 15개월 이내의 CLP 환아 3명과 유사한 나이대의 정상아 8명의 cry에 대한 공기역학 및 음향음성학적 분석을 통해 CLP 환아와 정상아, CLP환아의 술전, 술후 cry특성을 비교 분석하였다. 결과는 다음과 같다. 1 공기역학적 분석 1) airflow는 CLP 환아의 경우 정상아보다 약간 높았고 술 후 약간 증가하였다. 2)폐활량을 나타내는volume에서는 정상아보다 술전 CLP환자의 경우 보상적으로 더 큰 수치를 보였고 술후 약간 증가하였다. 3)강도를 나타내는 parameter(SPL)에서는 정상아 보다 술전 CLP환자의 계측치가 약간 작았으나 술 후 증가하는 양상을 보였다. 2. 음향음성학적 분석 1)기저 주파수 분석시 정상아에 비해 술 전 CLP환자의 경우 계측치가 약간 낮았으나 술 후 증가하여 정상군의 계측치에 근접하였다. 2)강도를 나타내는energy 측정시 정상아에 비해 술 전 CLP계측치가 보상성으로 약간 큰수치를 나타내었고 술 후 약간 더 증가하였다. 3) Shimmer에서는CUI환자의 술후계측치가술전에 비해 현저히 감소하여 정상군의 수치에 근접하였다.

• Korean Journal of Cleft Lip And Palate
• /
• v.4 no.2
• /
• pp.1-8
• /
• 2001

The dissimilarities between South and North Korea have persisted in spite of the reconciliation campaign by both countries. The situation of the cleft lip & palate of North Korea was very unclear until now. The purpose of this study is to understand all the current facts of cleft lip & palate in North Korea so that we can find ways of helping North Korea in this field of medicine. The present data and analysis are extracted from North Korean textbooks. The results are as follow. 1. In North Korea, patients with CLP are treated by oral surgeons or maxillofacial surgeons. The detailed contents about the CLP are well described in the North Korean textbooks for the dental students. 2. The terminology of CLP in North Korea has changed from time to time, but the present terminology not being so different from South Korean counterpart. So there will be no particular problems in mutual communication. 3. The main classification for CLP in North Korea originated from Kernahan & Stark's classification as is with South Korea. 4. The incidence of CLP is 1 : 1,000-1,200 in North Korea, which is lower than that of South Korea. There is, however, some difference between the North and South Korean CLP in detailed statistics. 5. We found the North Korean physicians have shown much interest in pursuing the etiology and the prevention of CLP. 6. The timing of CLP operations varied a lot in North Korea. There was recommendation by few for the operation in much late age than in South Korea. 7. The classical operation techniques of cleft lip have changed. For unilateral cleft lip Tennison-Randall method was replaced by Millard I method: and for bilateral cleft lip LeMesurier method was replaced by Veau III and Tennison methods. But for cleft palate Pushback palatoplasty has been utilized consistently.

• Journal of Microbiology and Biotechnology
• /
• v.33 no.9
• /
• pp.1130-1140
• /
• 2023

Among the AAA+ proteases in bacteria, FtsH is a membrane-bound ATP-dependent metalloprotease, which is known to degrade many membrane proteins as well as some cytoplasmic proteins. In the intracellular pathogen Salmonella enterica serovar Typhimurium, FtsH is responsible for the proteolysis of several proteins including MgtC virulence factor and MgtA/MgtB Mg²⁺ transporters, the transcription of which is controlled by the PhoP/PhoQ two-component regulatory system. Given that PhoP response regulator itself is a cytoplasmic protein and also degraded by the cytoplasmic ClpAP protease, it seems unlikely that FtsH affects PhoP protein levels. Here we report an unexpected role of the FtsH protease protecting PhoP proteolysis from cytoplasmic ClpAP protease. In FtsH-depleted condition, PhoP protein levels decrease by ClpAP proteolysis, lowering protein levels of PhoP-controlled genes. This suggests that FtsH is required for normal activation of PhoP transcription factor. FtsH does not degrade PhoP protein but directly binds to PhoP, thus sequestering PhoP from ClpAP-mediated proteolysis. FtsH's protective effect on PhoP can be overcome by providing excess ClpP. Because PhoP is required for Salmonella's survival inside macrophages and mouse virulence, these data implicate that FtsH's sequestration of PhoP from ClpAP-mediated proteolysis is a mechanism ensuring the amount of PhoP protein during Salmonella infection.

• ETRI Journal
• /
• v.20 no.3
• /
• pp.251-271
• /
• 1998

It is well known that if usage parameter control/network parameter control (UPC/NPC) functions are used together with a cell loss priority control scheme in ATM networks, the measurement phasing problem can occur. This makes it difficult for a network provider to define and commit the cell loss ratio as a QoS parameter. To solve the problem, we propose a new UPC/NPC algorithm. By using the proposed UPC/NPC algorithm, we can define the cell loss ratios for CLP = 0 and CLP = 0+1 cell streams without the measurement phasing problem under any conditions. We analyzed the performance of the proposed UPC/NPC algorithm. Using a discrete time model for the UPC/NPC architecture with a discrete-time semi-Markov process (DSMP) input model, we obtained the cell discarding probabilities of CLP = 0 and CLP = 0+1 cells streams and showed that more CLP = 0 cells are accepted compared to what was proposed in ITU-T.