• Journal of the Institute of Electronics Engineers of Korea SP
• /
• v.41 no.2
• /
• pp.1-10
• /
• 2004

This paper proposes the method that design CLUT(color look-up table) simultaneously processing gamut mapping and color space conversion using only CLUT without complex computation. After CLUT is constructed using scanner gamut and printer gamut, the scanner gamut is extended to include original scanner gamut. This extended scanner gamut is used as input CIE $L^{*}$ $a^{*}$ $b^{*}$ values for CLUT. Then CMY values are computed by using gamut mapping. Input RGB image of scanner is converted into CIE $L^{*}$ $a^{*}$ $b^{*}$ by using regression function. CIE $L^{*}$ $a^{*}$ $b^{*}$ values of scanner are converted into CMY values without computation of additional gamut mapping using the proposed CLUT. In the experiments, the proposed method resulted in the similar color difference, but reduced the complexity computation than the direct computing method to process gamut mapping and color space conversion respectively.espectively.ively.

• Biomedical Science Letters
• /
• v.8 no.4
• /
• pp.211-215
• /
• 2002

The baculovirus/Sf9 cell expression can be employed as a powerful system for producing large amounts of the human erythrocyte glucose transporter, GLUT1 heterologously In order to exploit the system further, it is necessary to develop a convenient method for demonstrating that the transporter expressed in insect cells is biologically active. To achieve this, we have expressed the human CLUT1 in insect cells and photolabelled the expressed protein with [$^3$H] cytochalasin B, a potent inhibitor of the human erythrocyte glucose transporter. Subsequently, the labelled proteins were analysed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Membranes labelled with [$^3$H] cytochalasln B in the presence of L-Glucose yielded a single sharp peak of labelling of apparent $M_r$ 45,000 on SDS/polyacrylamide gels. The mobility of this peak corresponded exactly to that of the band detected by anti-glucose transporter antibodies on Western blots of membranes prepared from insect cells infected with recombinant virus. In addition, the sharpness of the radioactive peak provides further evidence for the conclusion that the expressed protein is much less heavily and heterogeneously glycosylated than its erythrocyte counterpart. No peak of labelling was seen with the membranes prepared from non-infected Sf9 cells. Furthermore, the incorporation of label into this peak was completely inhibited by the presence of 500 mM-D-Glucose during tile photolabelling procedure, showing the stereoselectivity of the labelling. These evidences clearly show that human glucose transporter expressed in insect cells exhibits native-like biological activity, and that photolabelling with [$^3$H] cytochalasin B can be a convenient means for analysing the biological activity of the transport protein expressed in insect cells.

• JSTS:Journal of Semiconductor Technology and Science
• /
• v.16 no.1
• /
• pp.44-57
• /
• 2016

Rectification is used as a preprocessing to reduce the computation complexity of disparity estimation. However, rectification also requires a complex computation. To minimize the computing complexity, rectification using a lookup-table (R-LUT) has been introduced. However, since, the R-LUT consumes large amount of memory, rectification with compressed LUT (R-CLUT) has been introduced. However, the more we reduce the memory consumption, the more we need decoding overhead. Therefore, we need to attain an acceptable trade-off between the size of LUT and decoding overhead. In this paper, we present such a trade-off by adaptively combining simple coding methods, such as differential coding, modified run-length coding (MRLE), and Huffman coding. Differential coding is applied to transform coordinate data into a differential form in order to further improve the coding efficiency along with Huffman coding for better stability and MRLE for better performance. Our experimental results verified that our coding scheme yields high performance with maintaining robustness. Our method showed about ranging from 1 % to 16 % lower average inverse of compression ratio than the existing methods. Moreover, we maintained low latency with tolerable hardware overhead for real-time implementation.