• 대한전자공학회논문지SP
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• 제41권2호
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• pp.1-10
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• 2004

본 논문에서는 복잡한 연산을 거치지 않고 칼라 참조표만으로 색역 사상과 색공간 변환을 동시에 처리하는 칼라 참조표를 설계하였다. 스캐너와 프린터의 색역을 구성하는 참조표를 만들고 스캐너에서 계산된 색역 데이터를 색역 확장하여 칼라 참조표의 입력 CIEL/sup */a/sup */b/sup */ 값으로 사용한다. 칼라 참조표 생성을 위한 입력 CIEL/sup */a/sup */b/sup */ 값들은 가변 다중 닻점 색역 사상 방법을 사용하여 색역 사상된 CMY 값으로 계산된다. 제안한 칼라 참조표는 칼라 운영 시스템에 적용하여 스캐너 RGB 입력영상을 다항 회귀 방정식을 이용하여 CIEL/sup */a/sup */b/sup */ 색공간으로 변환한 후에 제안한 칼라 참조표를 이용하여 색역 사상과 동시에 색공간 변환을 처리하게 된다. 실험에서는 제안한 방법이 직접 계산에 의한 색역 사상 방법에 비해서 색차는 유사하면서 연상의 복잡도는 줄이는 결과를 얻었다.

• 대한의생명과학회지
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• 제8권4호
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• pp.211-215
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• 2002

The baculovirus/Sf9 cell expression can be employed as a powerful system for producing large amounts of the human erythrocyte glucose transporter, GLUT1 heterologously In order to exploit the system further, it is necessary to develop a convenient method for demonstrating that the transporter expressed in insect cells is biologically active. To achieve this, we have expressed the human CLUT1 in insect cells and photolabelled the expressed protein with [$^3$H] cytochalasin B, a potent inhibitor of the human erythrocyte glucose transporter. Subsequently, the labelled proteins were analysed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Membranes labelled with [$^3$H] cytochalasln B in the presence of L-Glucose yielded a single sharp peak of labelling of apparent $M_r$ 45,000 on SDS/polyacrylamide gels. The mobility of this peak corresponded exactly to that of the band detected by anti-glucose transporter antibodies on Western blots of membranes prepared from insect cells infected with recombinant virus. In addition, the sharpness of the radioactive peak provides further evidence for the conclusion that the expressed protein is much less heavily and heterogeneously glycosylated than its erythrocyte counterpart. No peak of labelling was seen with the membranes prepared from non-infected Sf9 cells. Furthermore, the incorporation of label into this peak was completely inhibited by the presence of 500 mM-D-Glucose during tile photolabelling procedure, showing the stereoselectivity of the labelling. These evidences clearly show that human glucose transporter expressed in insect cells exhibits native-like biological activity, and that photolabelling with [$^3$H] cytochalasin B can be a convenient means for analysing the biological activity of the transport protein expressed in insect cells.

• JSTS:Journal of Semiconductor Technology and Science
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• 제16권1호
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• pp.44-57
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• 2016

Rectification is used as a preprocessing to reduce the computation complexity of disparity estimation. However, rectification also requires a complex computation. To minimize the computing complexity, rectification using a lookup-table (R-LUT) has been introduced. However, since, the R-LUT consumes large amount of memory, rectification with compressed LUT (R-CLUT) has been introduced. However, the more we reduce the memory consumption, the more we need decoding overhead. Therefore, we need to attain an acceptable trade-off between the size of LUT and decoding overhead. In this paper, we present such a trade-off by adaptively combining simple coding methods, such as differential coding, modified run-length coding (MRLE), and Huffman coding. Differential coding is applied to transform coordinate data into a differential form in order to further improve the coding efficiency along with Huffman coding for better stability and MRLE for better performance. Our experimental results verified that our coding scheme yields high performance with maintaining robustness. Our method showed about ranging from 1 % to 16 % lower average inverse of compression ratio than the existing methods. Moreover, we maintained low latency with tolerable hardware overhead for real-time implementation.