• Title/Summary/Keyword: CLE

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Homeobox Gene (OSH1) Expression in Embryonic Mutants of Rice (Oryza sativa L.)

  • Hong, Soon-Kwan;Lee, Sang-Lyung;Shin, Young-Boum;Yoon, Kyung-Min;Kim, Nam-Soo
    • Animal cells and systems
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    • v.2 no.1
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    • pp.81-86
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    • 1998
  • Recent identification and characterization of plant homeobox genes suggest that they play important roles in morphogenetic events. OSH1, one of the rice homeobox genes, is thought to be related to organ development since the changes of OSH1 gene expression cause morphological abnormalities of leaves by the ectopic expression and is expressed during early embryogenesis. In this experiment, the expression pattern of OSH1 was analyzedinmutants by in situ hybridization, and OSH1's potential as a molecular marker was explored. Region-specific expression of OSH1 during early embryogenesis shows that OSH1 could be used as a molecular marker for characterizing embryo mutants. Although several organless and shootless mutants showed normal expression of OSM1, some mutants exhibited abnormal expression patterns. In a minute organless cle1-1 embryo whose epidermis resembled morphologically the epithelium of scutellum, OSH1 expression was limited to a small basal region. This expression pattern suggests the gross deletion of the basal part. In a radicleless mutant, odm115, OSH1 expression was detected in a basal region instead of subcentral region of the ventral side. Together with other characteristics (short embryo and normal adventitious roots), odm115 was estimated to be derived from the deletion of basal region. Among five shootless mutants, three showed normal expression of OSH1. In the shl2 embryo, no expression of OSH1 was observed. In the shl1 embryo, however, OSH1 expression was extended to a dorsal side, indicating that SHL2 might be related to dorsoventral patterning. The above results of in situ hybrydization clearly indicate that OSH1 can be utilized as a marker for characterizing gene functions of embryo mutants.

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A Study of Costumes in the $Lo{\ddot{i}}e$ Fuller's Dance (로이 풀러의 무대 의상에 관한 연구)

  • Choi, Yoo-Jin
    • The Research Journal of the Costume Culture
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    • v.16 no.5
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    • pp.878-890
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    • 2008
  • As a pioneer of modem dance, $Lo{\ddot{i}}e$ Fuller($1869\sim1928$) was important because she introduced a free style dance using a new style of stage costumes. Also, $Lo{\ddot{i}}e$ Fuller was an important motif in $fin-de-si{\grave{e}}cle$, French arts, posters, sculptures, and so forth. This study aimed to clarify the characteristics of Fuller's dance and costumes, and to analyze the modernism in both. To better understand Fuller's dance, this article talks about research on Fuller's career, dance, and Fuller's own biography. Stage costumes, letters for patent, and photographs were are also studied. The most important elements of Fuller's dance were her costumes and the electric lights which could make free style expressions a fantastic representation of dance. To clarify the modernity of Fuller's dance, first of all, this study researched Fuller's career in dance and then analyzed representations of Fuller's dances in visual arts; posters, sculptures and lastly, the characteristics of Fuller's stage costumes. As a result, this article analyzed characteristics of Fuller's dance in three points. First, Fuller used sculptures of silk cloth's drapery to express physical movements and actions. Second, Fuller used lighting and electrical effects that had just been developed in the late 19th Century. Third, Fuller made an application for patents against stage costume and stage mechanisms for her original shows. This study focused on $Lo{\ddot{i}}e$ Fuller's stage costumes which played a main role in expressing fantastic sculptures for the first time in costume, visual arts, and dance studies. This study clarifies the characteristics of the stage costumes in Fuller's dance and is estimated as pioneering and fundamental research.

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Analysis of Seismic Behaviour of Cut and Cover Tunnel according to backfill materials (뒤채움재 특성별 개착식 터널의 동적 거동 특성 연구)

  • Kim, Nag-Young;Lee, Seung-Ho;Lee, Yong-Jun;Kim, Jung-Ho
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.7 no.2
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    • pp.177-186
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    • 2005
  • World widely, the occurrences of earthquakes have been increased recently including to nations which earthquake often happen to like Japan. Taking into account that the earthquake acceleration at design was 0.154 g at the contigency level earthuake (CLE) with its geological condition, it resulted that this method must be the most efficient condition against the earthquake vibration to two lane cut-and cover tunnel. The correlations between the result of the shaking table test and its numerical analysis showed that the test data of the bending stress due to the earthquake vibration have been underestimated about 5 percents in case of the cut slope 1 : 0.6 and about 10 percent in case of the cut slope 1 : 1.2. However, on average, the results showed a similar tendency that the higher the height of the backfill of the EPS block was, the less the bending stress was.

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A study on electron beam lithography for 0.1$\mu\textrm{M}$ T-gate formation at P(MMA/MAA)/PMMA structure (PMMA/P(MMA/MAA) 구조에서 0.1$\mu\textrm{M}$ T-gate 형성을 위한 전자빔 리소그래피 공정에 관한 연구)

  • Choe, Sang-Su;Lee, Jin-Hui;Yu, Hyeong-Jun;Lee, Sang-Yun
    • Korean Journal of Materials Research
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    • v.5 no.1
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    • pp.96-103
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    • 1995
  • This art~cle reports on the formation of T - Gate with O.1$\mu$m foot and 0.4$\mu$m head width at PMMA/P( MMA/MAA) resist structure using a 30KV electron beam lithography system. From the result of Monte Carlo simulation on PMMA/P( MMA/MAA)/GaAs, we obtain the dissipation energy ratio of forwardscattered electron and backscattered electron within 0.1$\mu$m scattering radius is 19.5 : 1 0.1$\mu$m T - gate has been formed with 30KV gaussian electron beam at a 440$\mu C/\textrm{cm}^2$ dosage. The gamma value of PMMA and P(MMA/MAA) at MIBK : IPA=l : 1 developer was 2.3. The overlay accuracy(3$\sigma$) from mix-andmatch of optical stepper and Ekeam lithography system for fabricating HEMT device is accomplished below 0.1$\mu$m.

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Leaching Behavior of LD Slag

  • Kim, Hyung-Suek;Han, Ki-Hyun;M. S. Oh;Byeon, Tae-Bong
    • Proceedings of the IEEK Conference
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    • 2001.10a
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    • pp.526-531
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    • 2001
  • LD slag, that is, a by-product of steel making process, has been mainly used as land construction materials. Recently, the seashore application of LD slag was tried in Japan and Korea tut the reaction between LD slag and seawater was not studied yet. We tried to clarify the leaching reaction and/or mechanism of LD slag and the reaction between seawater and LD slag. We tried to apply these results to the decarbonization of seawater for seawater magnesia process. At first, LD slag was milled and classified into 5 grades, that is, (ⅰ)45${\mu}{\textrm}{m}$ under, (ⅱ)0.25~0.5mm (ⅲ)0.5~1mm(ⅳ)1~2mm, (ⅴ)2.36~3.35mm. These slags were leached in the distilled water. In case of 45${\mu}{\textrm}{m}$ under, the pH of the leached solution was over 12. The chemical analysis of leached solution showed that the $Ca^{+}$$^2$was main component and the S $i^{+}$$^4$was very low. On the other hand, the content of S $i^{+}$$^4$in leached solution was decreased with the increase of pH of this solution. The nearly pure calcium solution was made and the ultra high purity MgO could be made with this calcium solution. The leaching behavior of LD slag was different between the fine particle and coarse particle. The calcium was leached by bulk dissolution in the coarse particle and by surface controlled reaction in fine particle. The leaching rate was slow in coarse particle and fast in fine particle. Therefore, the high pH solution, that is, over 12, was obtained in fine particle.cle.e.

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In vivo Antioxidant Effects of Aralia elata Seemann Ethanol Extract Administered with Benzo($\alpha$) pyrene

  • Nam, Sang-Myung;Chung, Cha-Kwon
    • Preventive Nutrition and Food Science
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    • v.4 no.1
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    • pp.52-56
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    • 1999
  • This study has examined the effects of Aralia elata Seemann ethanol extract on antioxidant enzyme systems inrats along with benzo($\alpha$) pyrene(B(a)P) administration . The ethanol extract of Aralia elata Seemann (50mg/kg body wt.) was fed to rats for 4 weeks by stomach tubing. The extract administration increased antioxidant activities of glutathione sulfur transferase(GST) comparing to the control. also total superoxide dismutase(SOD) and Cu, Zn-SOD activities were stimulated. Catalase activities were increased by 50% with the extract feeding compared to the control . Combined administration of B($\alpha$)P and the extract increased GST activity in B($\alpha$) P group. Although total SOD acitivity was decreased , Cu, Zn-SOD was greately increased from 0.10unit to 0.18 unit and catalase activity also was increased compared to the group of B($\alpha$) P. GST activity in CLE group was 1.32 unit, increased by 33% comparing to the group CL of 0.99unit. Cu, Zn-SOD and catalase activities in thegroup fed high fat and ethanol extracts were increased by 25% and 39%, respectivley comparing to the group of high fat. In addition , total SOD was decreased but, Cu, Zn-SOD acitivity was increased from 0.09 unit to 0.18unit. Catalase activity was 76.05 unit in the group of B($\alpha$) P and extract comparing to 65.26 units in B($\alpha$)P group. Serum $\alpha$-tocopherol of rat was markedly increased by theextract. Administration of B9$\alpha$)P reduced $\alpha$-tocopherol levels in the serum, on the other hand, lard in the diet increased $\alpha$-tocopherol levels in the serum. The above results indicate that Aralia bud exerts antioxidant functions in vivo against B($\alpha$)P. Further research may be necessary for the identification fo the biologically active material.

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A Study on Pre-Service Teachers' Perception of Learning Environment in Earth Science with Using Virtual Reality (VR): An Exploratory Case (지구과학에서의 가상 현실의 사용에 따른 예비 과학교사의 학습환경 인식 연구: 시험적 적용)

  • Shin, Myeong-Kyeong;Kim, Hee-Soo;Kim, Jong-Heon
    • Journal of the Korean earth science society
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    • v.27 no.3
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    • pp.269-278
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    • 2006
  • In this study, we used Virtual Reality (VR) materials on an introductory earth science course consisted of thirty six pre-service science teacher program students. Before and after class an instrument of Constructivist Learning Environment Survey (CLES) was administered. The main focus of the CLES was to evaluate how the classroom was prepared for student centered learning environment. The pre and post tests of student perceptions regarding their learning environment were compared in six domains: personal relevance, critical voice, shared control, student negotiation, scientific uncertainty, and attitude. Questionnaire regarding the general perception of the VR materials was administered as well. How future science teachers valued the use of VR materials in their classrooms was found from this study. Based on these results, we intend to contribute for a more complete understanding of the potential of VR materials in achieving better learner-centered classroom environment.

The Experimental study of Persicae Semen and Caesalpiniae Lignum on the endotoxin induced thrombosis (도인(桃仁), 소목(蘇木)이 Endotoxin으로 유발(誘發)된 어혈병태(瘀血病態)모델에 미치는 영향(影響))

  • Jun, Chan-Yong;Park, Jong-Hyung;Han, Yang-Hi;Kim, Dong-U;Park, Se-Gi;Lee, Chung-Jung-Hye;Go, Jae-Chul;Choi, You-Kyung;Baek, Eun-Gi;Hong, Ui-Sil;Park, Ji-Yun;Go, Seung-Hi
    • The Journal of Internal Korean Medicine
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    • v.22 no.1
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    • pp.39-44
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    • 2001
  • Objective : This experimental study was carried out to investigate the effects of Persicae Semen(PS) and Caesalpiniae Lignum(CL) on the intravascular coagulation which is considered as thrombosis. Methods : Rats were pretreated orally with PS and CL extracts(PS: 8.45mg/100g, CL: 4.4mg/100g), and 1 hour later, to induce thrombosis, were given an endotoxin(0.01mg/100g) injection into the caudal vein. After 4hours we collected blood by cardiac puncture and measured the platelet count, the prothrombin time, the level of fibrinogen and the FDP(fibrinogen degradation product). Results : PS and CL supressed the decrease of the Platelet count and the prolongation of prothrombin time. In these factors, both groups showed significant effect. Both groups supressed the decrease of the fibrinogen level and the increase of the FDP level. But, only the PS group showed a significant effect on the fibrinogen level, and only the Cle CL group showed a significant effect on FDP level. Conclusions : PS had significant effects on the platelet count, the prothrombin time, and the FDP level. CL had significant effects on the platelet count, the prothrombin time, and the fibrinogen level. Therefore, PerSicae Semen and Caesalpiniae Lignum seem to be applicable to treating the diseases related to thrombosis.

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Validity and Reliability of a Korean version of the Nursing Students' Perception of Instructor Caring (K-NSPIC) (간호대학생이 지각한 임상실습현장지도자의 돌봄에 대한 한국어판 측정도구의 타당도와 신뢰도 분석)

  • Lee, Shinae;Park, Hyojung
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.12
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    • pp.218-226
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    • 2018
  • The purpose of this study was to examine the validity and reliability of the Korean version of the nursing students' perception of instructor caring (NSPIC) scale developed by Wade and Kasper for nursing students. We collected data from 219 nursing students in the 3rd to 4th year at three nursing colleges from June 4 to June 20, 2018. Data were analyzed using SPSS 21.0 and AMOS 21.0. An exploratory factor analysis with varimax rotation was performed, resulting in five factors (respectful sharing, confidence through caring, control versus flexibility, supportive learning climate, appreciation of life's meanings) with a total of 27 items. Confirmatory factor analysis supported good convergent and discriminant validities. In addition, the concurrent validity test confirmed that the K-NSPIC scale was a validity tool as the correlation of the clinical learning environment (CLE) scale appeared as r=.64 (p<.001). The Cronbach's alpha coefficient of the K-NSPIC was .88, and Cronbach's alpha coefficient for each of the five factors was .91, .86, .80, .76, and .85; internal consistency was confirmed. It is significant that the K-NSPIC proved applicable as a useful tool for assessing instructor caring. It is also expected that it will assist in the design of programs to improve the caring ability of instructors.

Increasing Root-mat Formation by Plant Growth Regulators in Machine Transplanting with Infant Seedling of Rice (생장조절제를 이용한 벼 기계이앙 어린모 맷트형성 촉진)

  • Kim, Je-Kyu;Kim, Young-Hyo;Lee, Moon-Hee;Park, Rae-Kyeong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.36 no.1
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    • pp.57-64
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    • 1991
  • This experiment was conducted to elucidate the effect of plant growth regulators (PGR) on the root -mat formation of infant seedling (8- to 10-day-old seedling) of rice in machine transplanting. The rice seeds of Odaebyeo were socked in water with different concentrations of PGRs for 48 hours at room temperature. Seeding rate was 220 g per seed tray (30x60x3cm). Metalaxyl (25% wettable powder) was used for a fungicide. Generally, the metalaxyl-treated seeds markedly promoted the root growth of the rice seedling, while tetracy-cle, pachlobutrazol and NTN -821 reduced the seedling height and root length, and thickened the shoot diameter at higher concentation levels. Tetracycle decreased root length of the rice seedlings but increased root number per seedling, and root-mat formation was poor. Whereas, metalaxyl concentrations of 200 and 1,000 ppm markedly increased root length and number of root hairs without decreasing root number, thus root-mat formation was excellent. The optimum concentration of metalaxyl seed treatment to increase the root-mat formation of infant seedling of rice was about 200 ppm. Metalaxyl seed treatment could be advanced one to two days of the duration of root-mat formation compared with control.

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