• Title/Summary/Keyword: CL/P

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Solubility, Emulsion Capacity, and Emulsion Stability of Protein Recovered from Red Crab Processing Water (홍게 가공회수 단백질의 용해도, 유화력 및 안정성)

  • Kim, Yong-Jin;Sin, Tae-Seon;O, Hun-Il
    • The Korean Journal of Food And Nutrition
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    • v.9 no.3
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    • pp.319-324
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    • 1996
  • The functional properties of protein recovered from red crab (Chitinonecetes opiiie) processing in water (RCP) were examined and compared with those of soybean protein isolate at pH 2~10 in water and NaCl solu5ion. The solubilities of RCP and SPI were miniumu at pH 4, the isoelectric point and increased significantly at lower or higher than pH 4. Solubilities in NaCl solution for both proteins decreased with incr NaCl concentration increase at all pH ranges. Emulsion capacity for both proteins was also minimum at pH 4 and increased as protein concentration increased from 2 to 6%. Emulsion capacity of RCP was higher than these of SPI at pH 6∼10 and all protein concentrations. Emulsion stability showed a similar trend to that of emulsion capacity. RCP had higher oft absorption capacity and lower water absorption capacity than SPI.

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Changes in the Membrane Properties of Zygosaccharomyces rouxii in Response to Osmotic Stress (삼투압 스트레스에 대응하는 Zygosaccharomyces rouxii의 막성질의 변화)

  • 구니아기호소노;류병호
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.22 no.1
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    • pp.101-107
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    • 1993
  • Zygosaccharomyces rouxii is a salt-tolerant yeast which plays an important role during the ripening stage of soy sauce fermentation. Z. rouxii used in the experiment could grow in YPD (1 % yeast extract, 2% peptone and 2% glucose, pH5.0) medium with 18% (w/v) NaCl, whereas Saccharomyces cerevisiae could only grow in YPD medium with less than 8% NaCl. In the presence of 15% NaCl, Z. rouxii accumulates a large amount of glycerol as a compatible solute within the cells in the exponential phase. It is a characteristic of salt-tolerant yeasts. From the chemical analyses on membrane lipid fluidity, the membrane structure of the cells grown in 15% NaCl was suggested to become more rigid and its fluidity was decreased to keep glycerol within the cells in response to surrounding medium with high concentrations of salt.

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Control Indian meal moth Plodia interpunctella by gas treatment

  • Han, Gyung Deok;Kwon, Hyeok;Jin, Hyun Jung;Kum, Ho Jung;Kim, Bo Hwan;Kim, Wook
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.45-45
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    • 2017
  • The Indian meal moth, Plodia interpunctella, is one of the most important pests of stored food in the food processing industry worldwide. To control the Indian meal moth, methyl bromide, phosphine, high carbon dioxide, sulfuryl fluoride and plant essential oil fumigation have been considered. However, these treatments have disadvantages. For example, depleting the ozone layer, showing resistance in insect, low control efficacy or need high cost for treatment. Chlorine dioxide ($ClO_2$) is strong disinfectant and insecticide. The gas caused a malfunction in enzymes. The oxidative stress induced by $ClO_2$ gas treatment damaged to a physiological system and all life stages of P. interpunctella. The gaseous $ClO_2$ is a convincing alternative to methyl bromide for controlling P. interpunctella. The gaseous $ClO_2$ was generated by a chlorine dioxide generator (PurgoFarm Co., Ltd., Hwasung, Korea). It generated highly pure $ClO_2$ gas and the gas blown out through a vent into a test chamber. Gas entry to the chamber was automatically controlled and monitored by a PortaSene II gas leak detector (Analytical Technology, Collegeville, PA, USA). The properly prepared eggs, larvae, pupae, and adults of P. interpunctella were used in this experiment. Data were analyzed using SAS 9.4. Percentage data were statistically analyzed after arcsine-root transformation. Analysis of variance was performed using general linear model, and means were separated by the least significant difference test at P < 0.05. Fumigation is an effective management technique for controlling all stages of P. interpunctella. We found that $ClO_2$ gas treatment directly effects on egg, larvae, pupae and adults of P. interpunctella. The gas treatment with proper concentration for over a day achieved 100 % mortality in all stages of P. interpunctella and short time treatment or low concentration gas treatment results showed that the egg hatchability, pupation rate, and adult emergency rate were lowered compare with untreated control. Also, abnormal pupae or adult rate were increased. Gaseous $ClO_2$ treatment induced insecticidal reactive oxygen species (ROS), and it resulted in fatal oxidative stress in P. interpunctella. Taken together, these results showed that exposure proper concentration and time of the gas control all stages of P. interpunctella by inducing fatal oxidative stress. Further studies will be required to apply the gas treatment under real-world condition and to understanding physiological reaction in P. interpunctella caused by oxidative stress.

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Optimal Temperature and Salt Concentration for Low Salt Dongchimi Juice Preparation (저염 동치미 쥬스의 제조를 위한 최적 발효온도 및 소금농도)

  • 엄대현;장학길;김종군;김우정
    • Korean journal of food and cookery science
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    • v.13 no.5
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    • pp.578-584
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    • 1997
  • Fermentation temperature and salt concentration of Dongchimi were studied for the development of low salt Dongchimi juice. The juice was prepared by soaking the radish in brine solution of 0.3∼3.0% and fermented at the temperature range of 10∼30$^{\circ}C$. The fermentation proceeded faster at higher temperature. However, the salt concentration effect was dependent on the temperature. Fermentation in 3.0% NaCl solution resulted the fastest reach to pH 3.8 followed by 0.5% NaCl at 10 and 20$^{\circ}C$, while higher NaCl concentration caused a decrease in the fermentation rate at 30$^{\circ}C$. Comparison of flavor of the juice of pH 3.9 showed that fresh sourness was high in the juice prepared at 20$^{\circ}C$ and in 0.5% NaCl. The preference test also showed the juice of pH 3.8∼4.0 fermented in 0.5% NaCl at 20$^{\circ}C$ to be the most preferable one. The salt concentration lower than 0.5% at 20$^{\circ}C$ resulted in faster fermentation and high values in turbidity. However 0.5% NaCl was scored high in flavor acceptability.

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Extracellular ATP Stimulates $Na^+\;and\;Cl^-$ Transport through the Activation of Multiple Purinergic Receptors on the Apical and Basolateral Membranes in M-1 Mouse Cortical Collecting Duct Cells

  • Jung, Jin-Sup;Hwang, Sook-Mi;Lee, Ryang-Hwa;Kang, Soo-Kyung;Woo, Jae-Suk;Kim, Yong-Keun
    • The Korean Journal of Physiology and Pharmacology
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    • v.5 no.3
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    • pp.231-241
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    • 2001
  • The mammalian cortical collecting duct (CCD) plays a major role in regulating renal NaCl reabsorption, which is important in $Na^+$ and $Cl^-$ homeostasis. The M-1 cell line, derived from the mouse cortical collecting duct, has been used as a mammalian model of the study on the electrolytes transport in CCD. M-1 cells were grown on collagen-coated permeable support and short circuit current $(I_{sc})$ was measured. M-1 cells developed amiloride-sensitive current $5{\sim}7$ days after seeding. Apical and basolateral addition of ATP induced increase in $I_{sc}$ in M-1 cells, which was partly retained in $Na^+-free$ or $Cl^--free$ solution, indicating that ATP increased $Na^+$ absorption and $Cl^-$ secretion in M-1 cells. $Cl^-$ secretion was mediated by the activation of apical cystic fibrosis transmembrane regulator (CFTR) chloride channels and $Ca^{2+}-activated$ chloride channels, but $Na^+$ absorption was not mediated by activation of epithelal sodium channel (ENaC). ATP increased cAMP content in M-1 cells. The RT-PCR analysis demonstrated that M-1 cells express $P2Y_2,\;P2X_3\;and\;P2Y_4$ receptors. These results showed that ATP regulates $Na^+$ and $Cl^-$ transports via multiple P2 purinoceptors on the apical and basolateral membranes in M-1 cells.

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Treatment of Industrial Wastewater by Electrochemical Method (전기화학적방법을 이용한 산업폐수 처리)

  • Cho, Ju-Sik;An, Myoung-Sub;Lee, Hong-Jae;Heo, Jae-Seon;Sohn, Bo-Kyoon;Heo, Jong-Soo
    • Korean Journal of Environmental Agriculture
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    • v.19 no.2
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    • pp.134-141
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    • 2000
  • For electrochemical treatment of industrial wastewater, the effects of voltage, distance between electrodes, initial pH and NaCl concentrations on removal of pollutants were investigated in a batch electrolysis system. Temperature and pH in electrochemical reactor increased with increase in supplied voltage, but no significant change in EC was found. Removal of COD, turbidity, T-N and T-P were also enhanced with increase in the voltage. On the conditions of short distances between electrodes and long electrochemical reaction times, it was found that COD and T-N were very effectively removed in the system. Regardless of the distances, more than 80% of turbidity and T-P were removed at the beginning of reaction. When initial pH of the wastewater was about 7, the highest efficiency of COD removal was found in the system. On the other hand, removal efficiency of turbidity was unlikely affected by initial pH of the wastewater. T-N removal was increased with increase in initial pH. T-P was successfully removed in the pH range of 5 to 9 with varying removal efficiency of 79 to 96% after 2 minutes of electrochemical reaction time. Addition of NaCl into the electrochemical reactor increased removal efficiency of electrochemical treatment. The highest removal efficiency of COD and T-P, turbidity and T-N was obtained at NaCl concentrations of 500mg/L, 1,000 mg/L and 500mg/L, respectively.

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Effect of Addition Levels of Sodium Chloride on Gel Properties of Surimi-like Pork (NaCl 첨가량에 따른 돈육 수리미의 젤 특성)

  • Kang Geun-Ho;Han Chul-Yong;Joo Seon-Tea;Kim Byoung-Chul;Park Gu-Boo
    • Food Science of Animal Resources
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    • v.26 no.1
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    • pp.20-27
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    • 2006
  • Effects of addition level of sodium chloride (NaCl) on gel properties of surimi-like pork (SLP) were investigated. Porcine semimembranosus muscle was used to manufacture SIP contained 1, 2, 3 and 4% NaCl to measure moisture content, pH, color, gel strength, micro-structure and sensory evaluation. The pH and moisture content of SLP were decreased as increasing of NaCl level. However, the gel strength of SLP was increased with increasing of NaCl level. Values of yellowness and chroma were lower in SLP of 2% and 3% NaCl compared with those of 1% and 4% NaCl. Amorphus protein particles size in micro-structure of SLP was decreased and coagulated as increasing level of NaCl. SLP of 1% NaCl had a structure formed by aggregates of densely packed globular proteins and arranged in clusters, whereas a well-structured matrix with a highly interconnected network of strand was observed in SLP of 4% NaCl. Result suggested that the increasing gel strength with NaCl level might be due to lower moisture content and denser micro-structure of gel.

Effect of Foliar Sprays of CaCl2 for Improving Fruit Quality of (복숭아 )

  • Kim, Ik-Youl;Kim, Mi-Young;Ru, Jong-Ho;Kim, Min;Lee, Yong-Se;Chang, Tae-Hyun
    • Korean Journal of Environmental Agriculture
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    • v.25 no.3
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    • pp.276-283
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    • 2006
  • To evaluate the effectiveness of the foliar spray of calcium on "Baekdo" peach fruit, we carried out experiments in the orchards. The sprays were applied with $CaCl_2\;(Ca:\;400mg.kg^{-1})\;and\;CaCl_2$ with adjuvants (amino acid, $2g.kg^{-1}$; phytic acid, $2ml.kg^{-1}$ and wood vinegar, $2ml.kg^{-1}$) for four times from June 12 through July 4 at weekly intervals. The fruits and leaves were evaluated for Ca content, firmness and incidence of Brown rot caused by Monilinia fructicola at harvest To evaluate fruit quality included Ca content, firmness natural decay during the storage, the fruits were stored at room temperature for 14 days. The Ca content in leaf and fruit flesh at harvest was significantly increased (P=0.05) in $CaCl_2$ + amino acid treatment among $CaCl_2$ treatments. However, there was not significant Ca content in fruit peel. The firmness of flesh increased significantly (P=0.05) in $CaCl_2$ + amino acid treatment. The natural decay (Rhizopus stolonifers) during storage at the room temperature for 14 days, the fruit treated with $CaCl_2$ + wood vinegar exhibited lowest (P=0.05) incidence. Also, the firmness of the fruit during storage was firmer with treated $CaCl_2$ than untreated fruit. In the treatments of $CaCl_2$ + phytic acid and $CaCl_2$ + amino acid, it was possible to reduce incidence of Brown rot caused by M. fructicola most effectively in the field. In addition, inoculation with M. fructicola in fruits was also the most effective treatment for inhibiting disease development in vitro. These results suggested that the foliar spray of $CaCl_2$ with adjuvants increased the content of Ca and firmness of the fruits positively. It also inhibited the natural decay and the Brown rot effectively.

내염성 효모의 분리 및 세포외 protease의 생산

  • Jeong, Seung-Chan;Hyeon, Gwang-Uk;Kim, Jae-Ho;Lee, Jong-Su
    • 한국생물공학회:학술대회논문집
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    • 2000.11a
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    • pp.559-562
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    • 2000
  • A halotolerant and extracellular pretense producing yeast was isolated from traditional Meju and identified as a strain of Hansenular sp. S-9 by investigation of its microbiological characteristics. The optimum pH, temperature and NaCl concentration for growth of Hansenular sp. S-9 were pH 7.5, $30^{\circ}C$ and 0.5M, respectively. The protease production from Hansenular sp. S-9 was maximized when it was grown on BD medium containing 1.0% beef extract, 1.0% glucose and 0.5M NaCl for 72 h at $30^{\circ}C$.

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An MO Study on Reaction Mechanism and Reactivity of p-Substituted Phenylchloroethylsulfones

  • Kim, Ui-Rak;Seo, Bae-Seok;Lee, Myoung-Jae
    • Nuclear Engineering and Technology
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    • v.9 no.1
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    • pp.33-38
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    • 1977
  • The molecular orbital theory (EHT) has been applied to the discussion of the reaction mechanism and reactivity of p-substituted phenyl-$\beta$-chloroethyl-sulfones, R-(equation omitted)-$SO_2CH_2CH_2Cl$, where R are $CH_3O\;,CH_3$, Cl, H. The theoretical conclusion derived are in good agreements with the experimental order.

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