• Journal of Periodontal and Implant Science
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• 제34권4호
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• pp.759-769
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• 2004

Periodontal therapy has dealt primarily with attempts at arresting progression of disease, however, more recent techniques have focused on regenerating the periodontal ligament having the capacity to regenerate the periodontium. The effect of chitosan, a carbohydrate biopolymer extracted from chitin, on periodontal ligament regeneration is of particular interest. The purpose of this study was to evaluate the effect of chitosan on the expression of extracellular matrix proteins in primary rat calvarial cells in Vitro. In the control group, cells was cultured with BGjb media. In the experimental groups, cells were cultured with chitosan in concentration of 0.01, 0.1, 1.0 and 2.0 mg/ml. Then each group was characterized by examining alkaline phosphatase activity at 3 and 7 days, and the ability to produce mineralized nodules of rat calvarial cells at 14 and 21 days. Synthesis of type I collagen (COL-I), osteocalcin (OCN), bone sialoprotein (BSP) was evaluated by RT-PCR at 14 days. The results were as follows: 1. Alkaline phosphatase activity was significantly higher in the concentration of chitosan 0.01mg/ml, 0.1mg/ml and 1.0mg/ml compared to control (p<0.05). 2. The percentage of mineralized bone nodule was more in the concentration of chitosan 0.1mg/ml and 1.0mg/ml than the control. 3. At 14 day culture, the expression of OCN was increased by chitosan in concentration of 1.0 mg/ml and 2.0 mg/ml. These results suggested that chitosan in concentration of 0.1 and 1,0 mg/ml stimulate the extracellular matrix of primary rat calvarial cells and may facilitate the formation of bone.

• Advances in concrete construction
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• 제9권4호
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• pp.387-395
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• 2020

This study has been carried out in two-phases to develop Fiber Reinforced Self-Compacting Concrete (FRSCC) performance. In the first phase, the composition of the quaternary composite binder compromised CEM I 42.5N (58-70%), Rice Husk Ash (25-37%), quartz sand (2.5-7.5%) and limestone crushing waste (2.5-7.5%) were optimized. And in the second phase, the effect of two fiber types (steel brass-plated and basalt) was investigated on the SCC optimized with the optimum CB as disperse reinforcement at 6 different ratios of 1, 1.2, 1.4, 1.6, 1.8, and 2.0% by weight of mix for each type. In this study, the theoretical principles of the synthesis of self-compacting dispersion-reinforced concrete have been developed which consists of optimizing structure-formation processes through the use of a mineral modifier, together with ground crushed cement in a vario-planetary mill to a specific surface area of 550 m2 / kg. The amorphous silica in the modifier composition intensifies the binding of calcium hydroxide formed during the hydration of C3S, helps reduce the basicity of the cement-composite, while reducing the growth of portlandite crystals. Limestone particles contribute to the formation of calcium hydrocarbonate and, together with fine ground quartz sand; act as microfiller, clogging the pores of the cement. Furthermore, the results revealed that the effect of fiber addition improves the mechanical properties of FRSCC. It was found that the steel fiber performed better than basalt fiber on tensile strength and modulus of elasticity; however, both fibers have the same performance on the first crack strength and sample destruction of FRSCC. It also illustrates that there will be an optimum percentage of fiber addition.

• Journal of Periodontal and Implant Science
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• 제35권2호
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• pp.277-288
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• 2005

There is a potential role of collagenase-3 in alveolar bone loss and periodontal disease progression, we need to develope or find chemotherapeutic drugs or herbal agents which may regulate the expression of MMP-13. Ginseng saponin, one of the major components of Korea ginseng(panax ginseng) root, has many various biologic effects, such as cytotoxic effect, tumoricidal effects, cytokine regulations, and protein biosynthesis effect. The purpose of this study was to determine the effects of Korea red ginseng saponin on MMP-13 gene expression in osteoblasts. The experimental groups were cultured with ginseng saponin in concentration of 1.0, 10, 25, 50, 100, 250 and $500{\mu}g/ml$ for MTT assay. Primary rat calvarial cells were pre-treated for 1 hour with ginseng saponin(100 ${\mu}g/ml$) and then stimulated with $IL-1{\beta}(1.0ng/ml)$ and PTH(10 nM). MMP-13 gene expression was evaluated by RT-PCR. The results were as follows: Ginseng saponin was cytotoxic to osteoblast at concentration exceeding $250{\mu}g/ml$ for longer than 24 hours in tissue culture(p<0.01). In RT-PCR analysis, steady state MMP-13 mRNA levels were increased approximately 350% by $IL-1{\beta}$, and 400% by PTH when normalized to untreated control. $IL-1{\beta}-indued$ MMP-13 mRNA expression was reduced 50% by pretreatment with ginseng saponin. But ginseng saponin didn't inhibit MMP-13 expression from PTH stimulated cells. This results suggest that ginseng saponin Inhibit $IL-1{\beta}-indued$ MMP-13 mRNA expression.

• 한국지반공학회논문집
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• 제22권4호
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• pp.85-94
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• 2006

본 논문에서는 고결(Cementation)에 의한 모래의 비배수 거동변화를 파악하기 위하여 석고를 고결유발제로 사용한 시료를 조성한 후 등방삼축시험(CIU)을 실시하였으며, 상대밀도 및 고결정도에 따른 거동 양상을 분석하였다. 연구결과 모래의 고결은 항복강도$(q_y)$, 항복시 할선탄성계수$(E_y)$, 첨두마찰각$(\Phi_p)$의 상당한 증가를 유발시키고, 상대밀도보다 모래의 거동에 더 큰 영향을 끼치는 것으로 확인되었다. 그러나 고결결합이 파괴된 이후, 모래의 거동은 고결보다는 상대밀도의 영향을 더 크게 받는 것으로 나타났다. 또한 고결결합에 의한 압축성 감소는 간극수압 발생율을 감소시켜 고결모래의 유효응력 경로가 미고결 모래의 전응력 경로쪽으로 편향되어 발생하였다. 고결결합이 파괴되기 전에는 다일레이션 경향이 감소하지만, 결합이 파괴된 후에는 미고결 모래보다 더 큰 다일레이션 경향이 유발되었다.

• 대한토목학회논문집
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• 제26권3C호
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• pp.181-190
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• 2006

본 연구에서는 석고함유율을 달리한 고결된 모래 시료를 조성하여 고결정도 및 상대밀도에 따른 고결모래의 거동양상을 분석하였다. 상대밀도 25, 40, 60%의 모래에 모래중량비 5~20%의 석고를 혼합하여 교반시킨 후 상재하중 55kPa 상태에서 양생하여 시료를 조성하였다. 조성된 고결시료는 일정한 구속압(200kPa)상태에서 비배수 전단시험(CIU)이 실시되었다. 실험 결과 고결정도의 증가에 따른 고결된 모래의 항복강도 및 강성의 증가양상을 확인하였고, 고결 결합이 파괴되기 전에는 다일레이션 경향이 억제되지만, 결합이 파괴된 직후 다일레이션 경향이 오히려 증가하는 것으로 나타났다. 또한 고결정도 및 상대밀도에 따라 간극수압 발생경향이 변하였으며, 고결에 의해 상전이선 및 파괴포락선에서의 유효응력비가 변하는 것을 유효응력경로 분석을 통하여 확인하였다. 일반적으로 상대밀도에 비해 고결정도가 고결된 모래의 거동에 더 큰 영향을 끼치는 것으로 나타났다.

• Journal of Periodontal and Implant Science
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• 제35권1호
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• pp.99-109
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• 2005

It has been focused on the importance of the host inflammatory response in periodontal pathogenesis and progression, treatment has been introduced to control the host response and the method, which diminishes production and activity of MMP by doxycycline, has been used in periodontal field. MMP is a proteolytic enzyme which plays a major role in tissue destruction and MMP-1 is secreted in the periodontally healthy tissue, while MMP-8, 9, 13, etc in the inflammatory state. Among these, MMP-13 has been discovered lately and reported to degrade primarily type II collagen. Periodontal ligament (PDL) cell plays a role in destruction of periodontal tissue. This study was to evaluate the effect of doxycycline and mefenamic acid, non-steroidal antiinflammatory drug on MMP-13 mRNA expression in the rat PDL cell. Doxycycline concentration of $1{\sim}100\;{\mu}g/ml$ was added rat PDL cell and cell activity was measured by MIT assay at day 1 and 3. MMP-13 gene expression was evaluated by RT-PCR after PDL cells were pre-treated for 1hour with doxycycline (50 ${\mu}g/ml$) alone or with mefenamic acid ($10^{-6}M$), then added $IL-1{\beta}$(1.0 ng/ml) and incubated for 16-18 hours. The results are as follows: 1. Cell activity decreased Significantly at 24 and 72 hours in 100 ${\mu}g/ml$ (p<0.05). 2. Level of MMP-13 mRNA was in 20.2% increase by $IL-1{\beta}$ and in pre-treating doxycycline group, expression of $IL-1{\beta}$ induced MMP-13 mRNA was inhibited by 31% than $IL-1{\beta}$ treated only. 3. Mefenamic acid did not inhibit on the expression of $IL-1{\beta}$ induced MMP-13 mRNA, while mefenamic acid in combination with doxycycline inhibited the expression by 41% compared to only $IL-1{\beta}$ stimulation. These results suggest that doxycycline synergistically inhibit the expression of $IL-1{\beta}$ induced MMP-13 mRNA in combination with mefenamic acid.