• Title/Summary/Keyword: CG-5

Search Result 583, Processing Time 0.025 seconds

The study on the anti-inflammatory effects of Phellodendri Cortex, Indigo Naturalis, Alumen, and Chinensis Galla, most frequently used for the external theraphy of Gu-Chang (구창(口瘡)의 외용약(外用藥)으로 다용(多用)되는 황백(黃柏), 청대(靑黛), 백반(白礬) 및 오배자(五倍子)의 항염작용(抗炎作用)에 관한 연구(硏究))

  • Choi, Kwan-Ho;Kim, Yong-Kook;Kang, Sung-Koo;Jung, Soon-Young;Jung, Jae-Ho;Seo, Hyung-Sik;Yoo, Jin-Gon
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
    • /
    • v.18 no.1
    • /
    • pp.183-198
    • /
    • 2005
  • This experimental study was performed to investigate the anti-inflammatory effects of Phellodendri Cortex(PC), Indigo Naturalis(IN). Alumen(AM), and Chinensis Galla(CG). These four prescriptions most frequently have been used in of oriental medicine for the external the theraphy of Gu-Chang. This study was done to evaluate the activity of superoxide dismutase(SOD) and the inhibitory effects of the formation of cyclooxygenase-2(COX-2), tumor necrosis $factor-{\alpha}(TNF-{\alpha}),\;interlenkin-1{\beta}(IL-1{\beta})$, prostaglandin-E2(PGE2) on the monocyte and neutrophil. The results were summerized as follows. 1. IN has no cytotoxicity but PC, AM, CG have a little cytotoxicity by the increase of concentrations. 2. PC, IN, AM and CG all inhibited the formation of COX-2 in the human neutrophil. 3. $1000{\mu}g/m{\ell}$ of PC increased the formation of SOD in the human monocyte. 4. $1000{\mu}g/m{\ell}$ of PC, $100{\mu}g/m{\ell}$ and $1000{\mu}g/m{\ell}$ of IN, $100{\mu}g/m{\ell}$ of CG inhibited the formation of $TNF-{\alpha}$ in the human monocyte. 5. $10{\mu}g/m{\ell}$ of PC, $1000{\mu}g/m{\ell}$ of IN inhibited the formation of $IL-1\;{\beta}$ in the human monocyte. 6. PC, IN, AM and CG all did not inhibit the production of PGE2 in the human monocyte In addition, the results show that PC, IN, AM and CG all have anti-inflammatory effects and can be used fer the external theraphy of Cu-Chang.

  • PDF

The Effect in the Muscle Function Following 8-Week Dead-lift training with Whole-body Vibration in Rehabilitation for Sports Players (8주간 데드리프트 운동 시 전신진동운동이 재활스포츠 선수의 근 기능 특성에 미치는 영향)

  • Oh, Ju-Hwan;Kang, Seung-Rok;Min, Jin-Young;Kwon, Tae-Kyu
    • Korean Journal of Applied Biomechanics
    • /
    • v.25 no.3
    • /
    • pp.343-351
    • /
    • 2015
  • Objective : The purpose of this study was to investigate the effects in the muscle function following 8-week dead-lift training with Whole-body Vibration(WBV) in rehabilitation for sports players. Method : Twenty young sports players. Each subjects were randomly assigned to a resistance training with Whole-body vibration group(TG, n=10), a resistance training without Whole-body vibration group(CG, N=10). The measurements which physical fitness test and joint torque test were performed before the randomization and after the 4-week and 8-week. The WBV group performed the dynamic Dead-lift exercise on a vibration platform during one minute. The CG group performed the equal training without vibration. The WBV and CON group repeated 5 set and trained two times weekly for 8-weeks. Paired t-test was used to test for differences between the groups at baseline and after 4-weeks and 8-Weeks. And independent t-test was used to test for differences between the groups at TG and CG. All analyses were executed using SPSS software 18.0. The level of significance was set at p<.050. Results : Following the 8-Weeks training sessions, an increase in the back-muscle strength was found to be greater for the TG compared with the CG group(p<.05). Muscle endurance was significantly decreased after training than before training only for the CG(p<.05). Isometric Hip/Lumber Extension/Flextion measurement was found to be significantly greater for the TG compared with the CG group(p<.05). The finding indicates that WBV effects as an efficient training stimulus to enhance muscle function by facilitating neural control trail. Following muscle activation in motor unit synchronization of the co-contraction of the muscles. Conclusion : The results imply that the WBV training may have enhanced muscle function in rehabilitation for sports players.

RADIOGRAPHIC COMPARATIVE STUDY OF FACIAL SKELETAL ASYMMETRY IN CRANIOMANDIBULAR DISORDER PATIENTS (두개하악장에 환자의 안면골 비대칭성에 관한 방사선사진상 비교분석)

  • Park Won-Kyl;Choi Eui-Hwan;Kim Jae-Duk
    • Journal of Korean Academy of Oral and Maxillofacial Radiology
    • /
    • v.24 no.2
    • /
    • pp.291-304
    • /
    • 1994
  • The purpose of this study was to analyze the facial asymmetry of the patients with the craniomandibular disorder. In this study, 50 patients, who have joint clicking and pain, mouth opening limitation, and 40 dental students, Chosun University, who did not posses any restoration and orthodontic treatment, joint clicking and pain, mouth opening limitation, were selected as the control group. Both the control group and the patient group were takened skull P-A, submento-vertex radiogram by standized methods. After that, the deviation and facial asymmetry were measured and analyzed. The results of the this study were as follows: 1. In the Skull P-A radiogram, the width difference of control group and patient group measured that the △ Cg-Go-Cl: control group were 3.35㎜, patient group were 4.51㎜ (P<0.05), the △Cg-Zy-Go: control group were 1.83㎜, patient group were 3.27㎜(P<0.001). 2. In the Skull P-A radiogram, the height difference of control group and patient group measured that the △ Cg-Go-Cl: control group were 131.85㎜, patient group were 188.45㎜(P<0.05), the △Cg-Zy-Go: control group were 1.58㎜, patient group were 2.68㎜(P<0.00l). 3. In the Skull P-A radiogram, the area difference of control group and patient group measured that the △ Cg-Go-Cl: control group were 120.76㎟, patient group were 185.49㎟(P<0.05), the △Cg-Zy-Go: control group were 2.29㎟, patient group were 3.37㎟(p<0.05). 4. In the submento-vertex radiogram, the width difference of control group and patient group measured that the △Mr-Cl-Ia: control group were 1.50㎜, patient group were 2.35㎜(P<0.05), the △Mr-Cm-Ia: control group were 1.75㎜, patient group were 3.17㎜(P<0.05), the △Mr-Go-Ia: control group were 1.96㎜, patient group were 3.24㎜(P<0.001), the △Mr-Cp-Co: control group were 1.74㎜, patient group were 2.73㎜(P<0.05). 5. In the submento-vertex radiogram, the height difference of control group and patient group measured that the △Mr-Cp-Ia: control group were 1.68㎜, patient group were 2.46㎜P<0.05), the △Mr-CI-Ia: control group were 2.38㎜, patient group were 3.74㎜(P<0.05), the △Mr-Co-Ia: control group were 1.63㎜, patient group were 2.80㎜(P<0.05), the △Mr-Cm-Ia: control group were 1.45㎜, patient group were 3.12㎜(P<0.001). 6. In the submento-vertex radiogram, the area difference of control group and patient group measured that the △ Mr-Cp-Ia: control group were 73.17㎟, patient group were 110.16㎟(P<0.05), the △Mr-Cl-Ia: control group were 105.09㎟, patient group were 180.87㎟(P<0.001), the △Mr-Co-Ia: control group were 103.31㎟, patient group were 148.48㎟(P<0.05), the △Mr-Cm-Ia: control group were 97.01㎟, patient group were 167.83㎟(P<0.05), the △Mr-Go-Ia: control group were 104.24㎟, patient group were 205.90㎟(P<0.05).

  • PDF

Effects of Swim Training on Tuberculosis infection in the Mouse Model at Different Temperature (온도 차이에 따른 수영훈련이 결핵균 감염정도에 미치는 영향)

  • Kwak Yi-Sub
    • Journal of Life Science
    • /
    • v.15 no.4 s.71
    • /
    • pp.652-656
    • /
    • 2005
  • Tuberculosis is the leading infectious disease in the world. It is urgent to develop new vaccine and treating drugs. Besides vaccines, we want to know the effects of regular swim training on TB infection in the mouse model. This study was designed to examine the effects of regular swim training on lung and spleen TB counts and $INF-\gamma$ activity in the trained mice at different temperature. The trained mice underwent a 10-wk endurance swim training (5 times/wk) in water at $29\~33^{\circ}C$ (WWG) and $21\~23^{\circ}C$(CWG) for 60 min. And they were divided into 3 groups according to the regular swim training (CG; control, WWG; warm water group, and CWG; cold water group). Mice were challenged by aerosol infection with M. tuberculosis H37Rv using an inhalation device (Glas-Col, Terre Haute, Ind.) calibrated to deliver bacteria into lungs. Three weeks after immunization, the mice were challenged. Four weeks after challenge, the mice were sacrificed and the numbers of viable bacteria in lung and spleen were determined by plating serial dilution of whole organ homogenates on nutrient Middlebrook 7H11 agar (Difco, Detroit, MI). Colonies were counted after four weeks incubation at $37^{\circ}C$. All data were expressed as mean, standard deviation by using SPSS package program (win 10.0). The result through the statistical analysis of this data were summarized as follows; In the weight changes, there were significant differences among CG, WWG, and CWG following the swim training at different temperature, and CWG was the lowest. In the change of $INF-\gamma$ following the swim training, there were significant differences (p<.05) among CG, WWG, and CWG after stimulated with media and CFP. In MTB counts, there were significant differences (p<.05) between CG and WWG in the lung. And also there were significant differences (p<.05) among CG, WWG, and CWG. These results suggest that regular swim training suppress Th1 immune response caused by decreased $INF-\gamma$ level in the WWG, Also For the WWG, highly increased level of TB counts appear in the lung and spleen compare to CG.

Effects of 17β-estradiol, Interleukin-1β, and Human Chorionic Gonadotropin on Activity and mRNA Expression of Plasminogen Activators in Porcine Endometrial Cells

  • Hwangbo, Yong;Cheong, Hee-Tae;Yang, Boo-Keun;Park, Choon-Keun
    • Development and Reproduction
    • /
    • v.22 no.2
    • /
    • pp.155-163
    • /
    • 2018
  • This study aimed to investigate changes in the activity and mRNA expression of plasminogen activators (PAs) induced by $17{\beta}$-estradiol ($E_2$), human chorionic gonadotropin (hCG), and interleukin-$1{\beta}$ ($IL-1{\beta}$) in porcine endometrial cells. Endometrial cells were isolated from the epithelium and cultured to 80% confluence. They were then treated for 24 h with $E_2$ (0.2, 2, 20, and 200 ng/mL), $IL-1{\beta}$ (0.1, 1, 10, and 100 ng/mL), and hCG (0.5, 1, 1.5 and 2 IU/mL). mRNA expressions of urokinase-type (uPA) and tissue-type (tPA) PAs were analyzed using reverse transcription PCR, and activities were measured using a PA activity assay. mRNA expressions of uPA and tPA increased with $E_2$ treatment; however, this was not significant. Similarly, treatment with hCG did not influence the mRNA expressions of PAs. Interestingly, treatment with 0.1 ng/mL $IL-1{\beta}$ significantly reduced the mRNA expression of uPA, but did not affect that of tPA. Treatment with 2, 20, and 200 ng/mL $E_2$ increased PA activity compared with the control group; treatment with 0.1 and 1 ng/mL $IL-1{\beta}$ significantly increased PA activity compared with the other $IL-1{\beta}$ treatment groups, whereas treatment with 10 and 100 ng/mL $IL-1{\beta}$ decreased. Treatment with 2 IU/mL hCG increased PA activity compared with the other treatment groups, although there were no significant differences between the hCG and control groups. In conclusion, the activity and mRNA expression of PAs were differently regulated by the hormone/cytokine and its concentration in porcine endometrial cells. Therefore, understanding PA regulatory mechanisms may help to improve the reproductive potential of domestic animals.

The Effects of Active Exercise Program using Sling on the Pain and Balance Following Total Knee Replacement (슬링을 이용한 능동 운동프로그램이 무릎 관절 전치환술 환자의 통증과 균형에 미치는 영향)

  • Ryu, Je-Ju;Jeong, Beom-Cheol;Yoo, Kyung-Tae
    • Journal of Convergence for Information Technology
    • /
    • v.12 no.2
    • /
    • pp.174-183
    • /
    • 2022
  • This study was conducted to investigate the effect of the active exercise program using sling on the pain and balance of total knee replacement patients. Subjects were 20 patients who received total knee replacement and are hospitalized, 10 patients in each group were randomly assigned to a group (CPM group) that applied only CPM (Continuous passive motion) and a group (CSG) that combined CPM with a active exercise program using sling. CG was performed CPM 5 days a week, CSG performed CPM 2 days a week and a active exercise program using sling 3 days a week, and each intervention was performed for 40 minutes a day for a total of 4 weeks. Pain was evaluated using VAS (Visual analog scale), and balance was measured using BT4 (Balance training 4) to measure C90 area, trace length, and Sway average velocity with eyes open and closed. As a result, there was a significant decrease in pain in both groups, and there was also a significant difference in the amount of change between groups. In balance, all variables except C90 of CG showed significant changes after intervention, and there was a significant difference between C90 and Vel with eyes closed in the amount of change between groups. Therefore, we believe that CPM and active exercise program using sling are effective interventions to reduce pain and improve balance in total knee replacement patients.

Effect of lonomycin and 6-Dimethylaminopurine on Oocyte Activation and Production of Rabbit Nuclear Transplant Embryos (Ionomycin과 6-Dimethylaminopurine이 토끼의 난자 활성화와 핵이식배 생산효율에 미치는 영향)

  • 하란조;강다원;최창용;윤희준;강태영;최상용;이효종;박충생
    • Journal of Embryo Transfer
    • /
    • v.13 no.1
    • /
    • pp.11-19
    • /
    • 1998
  • This study was to determine the effect of ionomycin and 6-dimethylaminopurine (6-DMAP) and/or elcetrical stimulation on the oocyte activation and production of rabbit nuclear transplant embryos. The oocytes were collected from the oviduct of superovulated rabbits at 14 h post hCG injection and cultured in TCM-199 containing 10% FBS until 19 h post hCG injection. To determine the optimum concentration and exposure time of 6-DMAP, some oocytes were activated with 5 $\mu$M ionomycin for 5 min and then in 2.0 mM 6-DMAP for 0.5 to 3.0 h, or in 1.0 to 3.0 mM 6-DMAP for 2.0 h. Other control oocytes were stimulated electrically(3X, 1.25 kV/cm, 60 $\mu$sec) in 0.3 M mannitol solution supplemented with 100 $\mu$M CaCl$_2$ and MgCl$_2$. The nuclear donor embryos of 8-cell stage were synchronized to G$_1$ phase of 16-cell stage, and the recipient cytoplasms were obtained from removal of the first polar body and a portion of membrane bound cytoplasm of the oocytes collected at 15 h post hCG injection. A separated blastomere was injected into the perivitelline space of the enucleated oocytes. The oocytes injected with nucleus were cultured until 19 h post hCG and then electrofused and activated by electrical stimulation with or without ionomycin and 6-DMAP. These nuclear transplant embryos were cultured in TCM-199 containing 10% FBS in 39˚C, 5% CO2 incubator for 120 h. For the oncytes activated parthenogenetically with electrical stimulation with or with-out ionomycin and the various concentration of exposure time of 6-DMAP, the highest cleavage(92.3%) and development to blastocyst stage(41.0%) were resulted from the oocytes activated by ionomycin and 2.0 mM 6-DMAP for 2.0 h, which were found to be significantly(P<0.05) higher than the cleavage(45.2%) and developement to blastocyst stage(14.3%) from the oocytes activated with electrical stimulation. The significantly(P<0.05) more oocytes(71.4%) developed to 4 cell stage at 24 h post activation by ionomycin and 6-DMAP than those by electrical stimulation(18.9%). For the nuclear transplant embryos, the cleavage rate was similarly high in oocyte activation by electrical stimulation with(79.4%) or without ionomycin and 6-DMAP(70.5%). However, the embryo development to blastocyst stage was significantly(P<0.05) higher in oocyte activation by electrical stimulation with ionomycin and 6-DMAP(44.4%) than by electrical stimulation only(25.0%). The significantly(P<0.05) more nuclear transplant embryos(45.6%) developed to 4 cell stage at 18 h post activation by electrical stimulation with ionomycin and 6-DMAP than those by electrical stimulation only(10.6%). These results indicated that the supplemental oocyte activation by ionomycin and 6-DMAP with electrical stimulation enhanced and accelerated the preimplanted in vitro development of the rabbit nuclear transplant embryos.

  • PDF

Short-term grazing behavior of cattle under indoor housing for a new-bred tetraploid ruzigrass (Brachiaria ruziziensis Germain et Everard)

  • Ishigaki, Genki;Nitthaisong, Pattama;Prasojo, Yogi Sidik;Kobayashi, Ikuo;Fukuyama, Kiichi;Rahman, Mohammad Mijanur;Akashi, Ryo
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.31 no.5
    • /
    • pp.748-754
    • /
    • 2018
  • Objective: The preference evaluation of cattle is an important factor for estimation and improvement of the grazing amounts of newly introduced or bred grasses or cultivars in barn. This study was performed to assess the grazing behavior (the amount of grazing and/or the grazing speed) of cattle as indirect method using newly bred Brachiaria ruziziensis tetraploid strain 'OKI-1'(BR) hay as treatment group and Cloris gayana 'Callide' (CG) hay as control group. It also compared the feasibility of using behavioral differences between two groups as one criteria for evaluating preference by Japanese black cattle in barn. Methods: Three experiments were carried out using 12 growing Japanese Black cattle including 6 males and 6 females. In each experiment, the four Japanese Black cattle (2 males and 2 females) were placed in separated stall and allowed to graze BR and CG in manger that was separated into two portions for about 30 min. The position and behavior of the cattle were recorded, and weighed the residual of each gay at 15 and 30 minutes after experiment start. Results: The BR was superior to CG in chemical composition such as protein, fibers and non-fibrous carbohydrate. The cattle, over all, tended to prefer BR over CG in the first half 15 minutes in terms of the time spent and amount of grazing. Additionally, growing cattle exhibited neophilia for BR bred newly. Conclusion: These findings indicated the current approach could be applied for one of criteria to evaluate the preference of hay by Japanese black cattle under indoor housing environment.

Wound healing effects of paste type acellular dermal matrix subcutaneous injection

  • Lee, Jin Ho;Kim, Jae-Won;Lee, Jun-Ho;Chung, Kyu Jin;Kim, Tae Gon;Kim, Yong-Ha;Kim, Keuk-Jun
    • Archives of Plastic Surgery
    • /
    • v.45 no.6
    • /
    • pp.504-511
    • /
    • 2018
  • Background Acellular dermal matrix (ADM) helps wound healing by stimulating angiogenesis, acting as a chemoattractant for endothelial cells, providing growth factors, and permitting a substrate for fibroblasts to attach. The current standard for using paste-type ADM (CG Paste) in wound healing is direct application over the wounds. The major concerns regarding this method are unpredictable separation from the wounds and absorption into negative-pressure wound therapy devices. This study aimed to investigate the effects of subcutaneous injection of paste-type ADM on wound healing in rats. Methods Full-thickness skin defects were created on the dorsal skin of rats. Eighteen rats were randomly divided into three groups and treated using different wound coverage methods: group A, with a saline dressing; group B, standard application of CG Paste; and group C, injection of CG Paste. On postoperative days 3, 5, 7, 10, and 14, the wound areas were analyzed morphologically. Histological and immunohistochemical tissue analyses were performed on postoperative days 3 and 7. Results Groups B and C had significantly less raw surface than group A on postoperative days 10 and 14. Collagen fiber deposition and microvessel density were significantly higher in group C than in groups A and B on postoperative days 3 and 7. Conclusions This study showed comparable effectiveness between subcutaneous injection and the conventional dressing method of paste-type ADM. Moreover, the injection of CG Paste led to improved wound healing quality through the accumulation of collagen fibers and an increase in microvessel density.

Attribution of Cortical Granules to Formation of Fertilization Envelopes and Polyspermy Block in Urechis unicinctus

  • Shin, Kil-Sang;Kwon, Hyuk-Jae;Kim, Wan-Jong
    • Animal cells and systems
    • /
    • v.9 no.2
    • /
    • pp.57-64
    • /
    • 2005
  • Cortical reaction and polyspermy block are well defined in most marine invertebrates. In Urechis species, the function of cortical granules (CGs) is not yet known, and there is controversy on whether the cortical reaction occurs, or the fertilization envelope (FE) is attributed to CG releases or functions to prevent polyspermy. This study was carried out to determine the cortical reactions and functions of the FE in Urechis unicinctus. Artificial insemination of the eggs revealed that CG release occurred to give rise to perivitelline space (PS) into the final FE. Both PS and final FE effectively blocked polyspermy. The final FE was accomplished within 10 min after sperm-egg initial binding. No massive release of CGs occurred within the early phase of 5 min after the initial binding, initially and the PS seemed to playa role to prevent polyspermy. The CG massively released its content into the PS in late phase of FE formation, and differentiated PS into five intermediate layers. The layers opened into each other by anastomosis, so that the final FE consisted of two layers, the inner layer ($15{\mu}m$ in thickness) and the outer layer ($1{\mu}m$ in thickness). The outer layer derived from vitelline layer and the inner layer consisted of PS and CG secretions. Immunofluorescence and confocal laser microscopy revealed that the spermatozoon took up residence in the egg cortex during FE formation and successive meioses of the fertilized egg. These results suggest that both PS and final FE of U. unicinctus were equivalent to the early and late block, respectively, of other marine animals.