• Imaging Science in Dentistry
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• v.34 no.3
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• pp.117-122
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• 2004

Purpose : To determine the proper image compression method and ratio without image quality degradation in intraoral digital radiographic images, comparing the discrete cosine transform (DCT)-based JPEG with the wavelet-based JPEG 2000 algorithm. Materials and Methods : Thirty extracted sound teeth and thirty extracted teeth with occlusal caries were used for this study. Twenty plaster blocks were made with three teeth each. They were radiographically exposed using CDR sensors (Schick Inc., Long Island, USA). Digital images were compressed to JPEG format, using Adobe Photoshop v.7.0 and JPEG 2000 format using Jasper program with compression ratios of 5 : 1,9 : 1, 14 : 1,28 : 1 each. To evaluate the lesion detectability, receiver operating characteristic (ROC) analysis was performed by the three oral and maxillofacial radiologists. To evaluate the image quality, all the compressed images were assessed subjectively using 5 grades, in comparison to the original uncompressed images. Results: Compressed images up to compression ratio of 14 : 1 in JPEG and 28 : 1 in JPEG 2000 showed nearly the same the lesion detectability as the original images. In the subjective assessment of image quality, images up to compression ratio of 9 : 1 in JPEG and 14 : 1 in JPEG 2000 showed minute mean paired differences from the original Images. Conclusion : The results showed that the clinically acceptable compression ratios were up to 9 : 1 for JPEG and 14 : 1 for JPEG 2000. The wavelet-based JPEG 2000 is a better compression method, comparing to DCT-based JPEG for intraoral digital radiographic images.

• Molecules and Cells
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• v.27 no.2
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• pp.225-235
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• 2009

Antibody phage display provides a powerful and efficient tool for the discovery and development of monoclonal antibodies for therapeutic and other applications. Antibody clones from synthetic libraries with optimized design features have several distinct advantages that include high stability, high levels of expression, and ease of downstream optimization and engineering. In this study, a fully synthetic human scFv library with six diversified CDRs was constructed by polymerase chain reaction assembly of overlapping oligonucleotides. In order to maximize the functional diversity of the library, a ${\beta}$-lactamase selection strategy was employed in which the assembled scFv gene repertoire was fused to the 5'-end of the ${\beta}$-lactamase gene, and in-frame scFv clones were enriched by carbenicillin selection. A final library with an estimated total diversity of $7.6{\times}10^9$, greater than 70% functional diversity, and diversification of all six CDRs was obtained after insertion of fully randomized CDR-H3 sequences into this proofread repertoire. The performance of the library was validated using a number of target antigens, against which multiple unique scFv sequences with dissociation constants in the nanomolar range were isolated.

• BMB Reports
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• v.33 no.3
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• pp.249-255
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• 2000

The Haemophilus influenzae type b (Hib) has been a major cause of bacterial meningitis in children who are less than two years old. The variable (V) region repertoire of adult Caucasian antibodies (Abs) to Hib polysaccharide (PS) has been characterized well. The majority of adult antibodies against Hib uses VL that is derived from the Vk gene A2 and have arginine at the N region. In order to explore the possibility those antibody responses to Hib-PS is variable in various age groups, we examined the VL regions of the antibodies to Hib-PS in Korean adults and children. We immunized Korean adults (n = 8) and children (n = 39) with Hib tetanus conjugated vaccines, isolated RNAs from the peripheral lymphocytes, and amplified the A2-derived VL regions by RT-PCR. The PCR products were subcloned and sequenced. Forty-seven out of 54 independent clones from children used the $J{\kappa}2$, or $J{\kappa}3$ gene in preference. The adults, however, used all of the $J{\kappa}$ genes evenly. With respect to the amino acid sequences of variable regions, adult $A2-J{\kappa}$ recombinants have a germline sequence. But, the 76th codon (AGC) of child $A2-J{\kappa}2$ recombinants was substituted with CGC (arginine) in most cases (88 %) and 77 percent of child clones using the $A2-J{\kappa}3$ genes have isoleucine-109 at the junction of $J{\kappa}-C{\kappa}$ instead of threonine that is found in a germline sequence. These results suggest that the mechanism of antibody production in young children is different from that of adults.

• Proceedings of the Korean Vacuum Society Conference
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• 2015.08a
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• pp.255-255
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• 2015

국제핵융합실험로(ITER)의 3대 목표 중 하나는 핵융합로 개발을 위한 삼중수소증식블랑켓 개념을 시험하고 검증하는 것이며, 이를 위해 시험증식블랑켓(TBM, Test Blanket Module) 프로그램을 마련, 각국이 참여할 수 있도록 하고 있다. 한국도 2012년 국가핵융합위원회 결정에 따라, EU, 일본, 중국, 인도와 함께 TBM 프로그램에 참여하고 있으며, 2021년 설치를 목표로 헬륨냉각 고체증식재 개념의 HCCR (Helilum Cooled Ceramic Reflector) TBM을 설계, 개발하고 있다. 한국형 TBM은 총 4개의 서브모듈과 하나의 후벽(Back Manifold, BM) 으로 구성되며, 각 서브모듈은 플라즈마와 대면하는 일차벽(First Wall, FW), 증식재와 증배재, 반사재를 담고 있는 증식영역(Breeding Zong, BZ), 냉각재 매니폴드 및 구조물 역할을 하는 측벽(Side Wall, SW) 등의 기능부품으로 구성되어 있다. 냉각재는 8 MPa, $300-500^{\circ}C$의 고온고압헬륨을 사용하고, Li2SiO4 혹은 Li2TiO4 형태의 Li 세라믹 증식재를 사용하며, 중성자 증배를 위해 Be 증배재 및 흑연 반사재를 사용한다 [1-3]. 2015년 2월 개념설계검토(CDR, Conceptual Design Review)를 위해, TBM-shield를 포함한 TBM-set 설계가 완료되었으며, 열수력, 구조, 지진, 전자기, 복합하중에 대한 평가가 진행되었다. 본 논문에서는 이 중 H/He-phase에 시험될 EM-TBM과 D-T phase에 시험될 INT-TBM에 대한 열수력 성능 결과를 소개하였다[5]. 각각의 열부하 조건은 0.17과 $0.3MW/m^2$이며, 중성자 조사는 D-T phase 에서만 고려되었다. 구조재 및 사용된 기능소재별 온도 요건을 정의하고, 성능해석 결과와 비교하였으며, 이를 통해 모든 온도 요건을 만족함을 최종 확인하였다. 이러한 온도 분포는 열응력 평가를 위해 구조해석 입력자료로 활용되었다.

• Journal of The Korean Society of Agricultural Engineers
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• v.59 no.3
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• pp.29-39
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• 2017

In this study, the GPM (Global Precipitation Mission) IMERG (Integrated Multi-satellitE retrievals for GPM) rainfall data was verified and evaluated using ground AWS (Automated Weather Station) and radar in order to investigate the availability of GPM IMERG rainfall data. The SPI (Standardized Precipitation Index) was calculated based on the GPM IMERG data and also compared with the results obtained from the ground observation data for the Hoengseong Dam and Yongdam Dam areas. For the radar data, 1.5 km CAPPI rainfall data with a resolution of 10 km and 30 minutes was generated by applying the Z-R relationship ($Z=200R^{1.6}$) and used for accuracy verification. In order to calculate the SPI, PERSIANN_CDR and TRMM 3B42 were used for the period prior to the GPM IMERG data availability range. As a result of latency verification, it was confirmed that the performance is relatively higher than that of the early run mode in the late run mode. The GPM IMERG rainfall data has a high accuracy for 20 mm/h or more rainfall as a result of the comparison with the ground rainfall data. The analysis of the time scale of the SPI based on GPM IMERG and changes in normal annual precipitation adequately showed the effect of short term rainfall cases on local drought relief. In addition, the correlation coefficient and the determination coefficient were 0.83, 0.914, 0.689 and 0.835, respectively, between the SPI based GPM IMERG and the ground observation data. Therefore, it can be used as a predictive factor through the time series prediction model. We confirmed the hydrological utilization and the possibility of real time drought monitoring using SPI based on GPM IMERG rainfall, even though results presented in this study were limited to some rainfall cases.

• Journal of Microbiology and Biotechnology
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• v.24 no.3
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• pp.408-420
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• 2014

Yeast surface-displayed antibody libraries provide an efficient and quantitative screening resource for given antigens, but suffer from typically modest library sizes owing to low yeast transformation efficiency. Yeast mating is an attractive method for overcoming the limit of yeast transformation to construct a large, combinatorial antibody library, but the optimal conditions have not been reported. Here, we report a large synthetic human Fab (antigen binding fragment) yeast surface-displayed library generated by stepwise optimization of yeast mating conditions. We first constructed HC (heavy chain) and LC (light chain) libraries, where all of the six CDRs (complementarity-determining regions) of the variable domains were diversified mimicking the human germline antibody repertoires by degenerate codons, onto single frameworks of VH3-23 and $V{\kappa}1$-16 germline sequences, in two haploid cells of opposite mating types. Yeast mating conditions were optimized in the order of cell density, media pH, and cell growth phase, yielding a mating efficiency of ~58% between the two haploid cells carrying HC and LC libraries. We constructed two combinatorial Fab libraries with CDR-H3 of 9 or 11 residues in length with colony diversities of more than $10^9$ by one round of yeast mating between the two haploid HC and LC libraries, with modest diversity sizes of ${\sim}10^7$. The synthetic human Fab yeast-displayed libraries exhibited relative amino acid compositions in each position of the six CDRs that were very similar to those of the designed repertoires, suggesting that they are a promising source for human Fab antibody screening.

• Journal of Auto-vehicle Safety Association
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• v.12 no.3
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• pp.52-60
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• 2020

Usage of EDR(Event Data Recorder) report for traffic accident analysis is currently increasing due to government regulation of EDR data release. Nevertheless, a lot of investigators simply adopt by comparing the number of ignition cycles(crash) at event to the number of ignition cycles(download) without an exact judgment whether event data occurred by this accident or not. In the EDR report, besides ignition cycles, there are many factors such as event record type, algorithm active(rear/rollover/side/frontal), time between events, event severity status(rollover/rear/right side/reft side/frontal), belt switch circuit status, driver/passenger pretensioner/air-bag deployment, PDOF(Principal Direction of Force) by ΔV to be able to decide whether or not to adopt. also the event data is considered enough to vehicle damaged state, accident situation at the scene of the accident. and there is described in "all data should be examined in conjunction with other available physical evidence from the vehicle and scene" in the CDR(Crash Data Retrieval) report. Therefore many investigators have to decide whether or not to adopt after they consider sufficiently to above factors when they are the traffic accident analysis and investigate the causes of a accident on the adopted event data. In this paper, we report to traffic accident investigators notable points and analysis methods on the basis of thousands of cases and the results of one's own experiment in NFS(National Forensic Service).

• Bulletin of the Korean Space Science Society
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• 2009.10a
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• pp.46.3-46.3
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• 2009

The SAR (Synthetic Aperture Radar) satellite has the advantage of implementing the imaging mission even though it is night time, cloudy weather, and all weather conditions, which is different from the satellite with the optical payload. This is the reason why the SAR satellite comes into the spotlight in the observation satellite field. The Korea Aerospace Research Institute (KARI) has been developing the first Korean SAR satellite and is currently integrating and testing the Flight Model. For the launch vehicle service, KARI finalized the selection of the launch vehicle service provider and finished Critical Design Review (CDR) of the interface between the bus and the launch vehicle. KARI and launch vehicle service provider also finished the test of the telemetry interface between the bus and the launch vehicle. The test of the telemetry interface has the purpose of checking the interface of the telemetry which is the SOH(State-of-Health) of the satellite in an early launch stage. For this test, KARI has finished the development of the spacecraft simulator which is composed of the bus simulator to generate the analog telemetry and the launch vehicle simulator to gather the telemetry. In this research, the result of the hardware implementation and the software implementation for the spacecraft simulator were described. Finally the results of the launch vehicle telemetry MUX test which were performed at the launch vehicle provider's design office by using the spacecraft simulator were summarized. It is expected that this simulator will be used in the next test after the manufacture of the launch vehicle.

• Journal of Preventive Medicine and Public Health
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• v.35 no.4
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• pp.313-321
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• 2002

Objectives : To identify the risk factors of dementia among the elderly in a large city. Methods : A cross-sectional study was conducted in July 2001, with potential participants selected by stratified two stage cluster sampling of the elderly population of Keumgog dong, Busan. A total of 452 elderly people aged 65 years and over, underwent a two phase diagnostic procedure. Mini-mental State Examination-Korean (MMSE-K) and Samsung Dementia Questionnaire were used for the 1st stage, and the Clinical Dementia Rating Scale (CDR), the Bartel ADL, and IADL Index, the Korean Geriatric Depression Scale (KGDS), the Modified Hatchinski Ischemic Scale (MHIS), and other laboratory tests were used for the 2nd stage. Results : Of the 446 participants finally chosen, 45 were confirmed with dementia, and 363 as normal, with the rests not confirmed with dementia or as normal, were excluded from the analysis. According to the logistic regression analysis, the risk of dementia was significantly higher In: people aged 80 and above (OR=4.36, 95% CI=1.97-9.62), illiterate (OR=3.58, 95% CI=1.71-7.46), who had a history of strokes (OR=6.35, 95% CI=2.71-14.87), or who had 3 history of hyperlipidemia (OR=4.74, 95% CI=1.65-13.61), compared to their counterparts. Conclusions : These results suggest that efforts to prevent strokes and hyperlipidemia can significantly decrease the risk of dementia.

• Journal of the Korea Society for Simulation
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• v.28 no.3
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• pp.25-40
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• 2019

Today, container terminals are fiercely competing to attract an increasing number of containers. As a way to improve terminal productivity, this study proposes two dispatching rules for yard truck allocation priorities. First, Multi-Attribute Dispatching Rule(MADR) is an allocation method to calculate the weighted sum of multiple factors affecting container terminal productivity and priority them. Especially, the workload of the quay crane was considered one of the factors to reduce the residence time of the ship. Second, Cycling Dispatching Rule(CDR) is the effective way to increase the number of double cycles that directly affect terminal productivity. To identify the effects of combinations of pooling and dispatching, a comparative experiments was performed on 8 scenarios that combined them. A simulation environment has been developed for experiments and the results have demonstrated that the combination of terminal level pooling and Multi-attribute Dispatching could be an excellent combination in KPIs consisting of GCR and delayed departure of ships, etc.