• Applied Biological Chemistry
• /
• v.39 no.6
• /
• pp.494-500
• /
• 1996

Uptake of hen metal ions by water dropwort (Oenanthe stolonifera DC.) and its cadmium-binding protein were studied to probe for good method to remove heavy metal contaminants from environments. The plant was cultured in the culture medium (pH 7.0) containing the various concentrations of $Cd^{2+}$, $Cr^{3+}$ or $Pb^{2+}$, for 3 and 7 days. The residual heavy metals deposited in roots linearly increased as the metal ions concentration increased up to 17 ppm for $Cd^{2+}$, 20 ppm for $Cr^{3+}$ and 50 ppm for $Pb^{2+}$. Above these concentrations, the plant growth was inhibited and the uptake rates of the metal ions decreased. The heavy metals absorbed by the plant were mostly deposited in roots. In particular, the residual concentration of lead in roots was about four times higher than those of cadmium and chromium. When cultured in the medium containing 20 ppm of each metal ion, 80% of cadmium, 90% of cromium and 96% of lead were deposited in roots out of the total residual metal ions in the plant. These values correspond to 6.1 mg of cadmium, 5.2 mg of chromium and 23.6 mg of lead per one gram of roots tissue on a dry weight basis. A cadmium-binding protein was partially purified by extraction, gel filtration and DEAE-Cellulose chromatography from water dropworts that was grown in the medium containing 20 ppm $Cd^{2+}$. The purified protein was a single band on SDS- and non-denaturing- polyacrylamide gel electrophoresis. Its molecular mass was estimated to be ca. 5,000 dalton by gel filteration. Analysis of amino acid composition of the protein indicated that it had a typical amino acid composition of heavy metal-binding protein in that it contained 27% of acidic amino acids and 9.9% of cysteine. However, it is likely that the protein is a new plant metal-binding protein, since its amino acid composition is somewhat different from those of phytochelatins that have been known so far.

• Korean Journal of Environmental Agriculture
• /
• v.16 no.4
• /
• pp.394-398
• /
• 1997

Changes of nutrient material and heavy metals of irrigation water and natural supplies by irrigation water into 1.0ha of paddy field during the rice cultivation was investigated pH of irrigation water ranged 6.458.34 and electrical conductivity showed $110{\mu}S/cm$ in the first irrigation period and commonly showed $90{\mu}S/cm$. The content of T-N, NH4-N and $NO_3-N$were 3.09~12.36, 0.17~1.34 and 2.70~10.72mg/L respectively. The content of T-P was 0.09~0.22mg/L and ortho-P was not detected. The content of $Ca^{++},\;Mg^{++},\;Na^+\;and\;K^+$ showed 0.89~5.12, 1.12~2.76, 1.74~4.52 and 1.34~4.18mg/L regardless of irrigation season respectively. Only Cu and Zn of heavy metals were detected 0.001~0.009 and 0.007~0.091mg/L, but Pb, Cd, Cr and Ni were not detected When supplied $4,250m^3$ of an irrigation water into 1.0㏊ of paddy field, natural supplies of nutrient materials were 29.67㎏ of T-N, 1.80㎏ of $NH_4-N$, 24.57kg of NO3-N and 0.51kg of T-P. Also $Ca^{++},\;Mg^{++},\;Na^+,\;K^+,\;Cl^-\;and\;SO_4\;^{2-}$ were supplied 9.42, 7.09, 13.34, 9.08, 40.27 and 41.38kg respectively. Only Cu and Zn of heavy metals were supplied 0.017kg of Cu and 0.133kg of Zn, Pb, Cd, Cr and Ni were not supplied

• Korean Journal of Food Science and Technology
• /
• v.43 no.5
• /
• pp.525-531
• /
• 2011

This study was conducted to examine the contents of lead (Pb), cadmium (Cd), arsenic (As), and mercury (Hg) in meat and meat products in Korea. The contents of Pb, Cd, As, and Hg in 466 samples of beef, pork, chicken, duck, ham, and sausage were measured using inductively coupled plasma mass spectrometry or a mercury analyzer. Wet ashing and microwave method were compared, and the recovery and reproducibility of the microwave method were better than those of wet ashing for meat and meat products. The recovery of the microwave method was 98.1% for Pb, 104.6% for Cd, and 103.4% for As, respectively. The best result was obtained through digestion using an acid mixture ($HNO_3$/$H_2O_2$, 6:2). Hg content was measured using a mercury analyzer. As a result, the contents of Hg and Cd in samples were lower than those of Pb and As. The average contents of Pb were 0.009 mg/kg in beef, 0.010 mg/kg in pork, 0.006 mg/kg in chicken, 0.007 mg/kg in duck, 0.005 mg/kg in ham, and 0.009 mg/kg in sausage. The average Cd contents were 0.0004 mg/kg in beef, 0.0004 mg/kg in pork, 0.0005 mg/kg in chicken, 0.0012 mg/kg in duck, 0.0015 mg/kg in ham, and 0.0019 mg/kg in sausage. The average As contents were 0.016 mg/kg in beef, 0.004 mg/kg in pork, 0.021 mg/kg in chicken, 0.010 mg/kg in duck, 0.014 mg/kg in ham, and 0.018 mg/kg in sausage. The average Hg contents were 0.713 ${\mu}g/kg$ in beef, 0.902 ${\mu}g/kg$ in pork, 0.710 ${\mu}g/kg$ in chicken, 0.796 ${\mu}g/kg$ in duck, 1.141 ${\mu}g/kg$ in ham, and 1.052 ${\mu}g/kg$ in sausage. Based on the results of the National Health and Nutrition Survey 2005, the levels of dietary exposure to heavy metal contaminants in meat and meat products were compared with the provisional tolerable weekly intake(PTWI) established by the Joint FAO/WHO Expert Committee on Food Additives. The average dietary exposure of the general population from meat and meat products was 0.03-0.2% of PTWI for Pb, Cd, As, and Hg, which indicates a safe level for public health at present.

• Asian Pacific Journal of Cancer Prevention
• /
• v.15 no.1
• /
• pp.467-474
• /
• 2014

Objective: To observe the effects of allogeneic and autologous transfusion on cellular immunity, humoral immunity and secretion of serum inflammatory factors and perforin during the perioperative period in patients with malignant tumors. Methods: A total of 80 patients (age: 38-69 years; body weight: 40-78 kg; ASA I - II) receiving radical operation for gastro-intestinal cancer under general anesthesia were selected. All the patients were divided into four groups based on the methods of infusion and blood transfusion: blank control group (Group C), allogeneic transfusion group (group A), hemodiluted autotransfusion Group (Group H) and hemodiluted autotransfusion + allogenic transfusion Group (A+H group). Venous blood was collected when entering into the surgery room ($T_0$), immediately after surgery ($T_1$) and 24h ($T_2$), 3d ($T_3$) and 7d ($T_4$) after surgery, respectively. Moreover, flow cytometry was applied to assess changes of peripheral blood T cell subpopulations and NK cells. Enzyme linked immunosorbent assays were performed to determine levels of IL-2, IL-10, TNF-${\alpha}$ and perforin. Immune turbidimetry was employed to determine the changes in serum immunoglobulin. Results: Both CD3+ and NK cells showed a decrease at $T_1$ and $T_2$ in each group, among which, in group A, CD3+ decreased significantly at $T_2$ (P<0.05) compared with other groups, and CD3+ and NK cell reduced obviously only in group A at $T_3$ and $T_4$ (P<0.05). CD4+ cells and the ratio of D4+/CD8+ were decreased in groups A, C and A+H at $T_1$ and $T_2$ (P<0.05). No significant intra- and inter-group differences were observed in CD8+ of the four groups (P<0.05). IL-2 declined in group C at $T_1$ and $T_2$ (P<0.05) and showed a decrease in group A at each time point (P<0.05). Moreover, IL-2 decreased in group A + H only at $T_1$. No significant difference was found in each group at $T_1$ (P<0.05). More significant decrease in group ?? at $T_2$, $T_3$ and $T_4$ compared with group A (P<0.05), and there were no significant differences among other groups (P>0.05). IL-10 increased at $T_1$ and $T_2$ in each group (P<0.05), in which it had an obvious increase in group A, and increase of IL-10 occurred only in group A at $T_3$ and $T_4$ (P<0.05). TNF-${\alpha}$ level rose at $T_1$ (P<0.05), no inter- and intra-group difference was found in perforin in all groups (P<0.05). Compared with the preoperation, both IgG and IgA level decreased at $T_1$ in each group (P<0.05), and they declined only in Group A at $T_2$ and $T_3$ (P<0.05), and these parameters were back to the preoperative levels in other groups. No significant differences were observed between preoperative and postoperative IgG and IgA levels in each group at $T_4$ (P>0.05). No obvious inter- and intra-group changes were found in IgM in the four groups (P>0.05). Conclusions: Allogeneic transfusion during the perioperative period could obviously decrease the number of T cell subpopulations and NK cells and the secretion of stimulating cytokines and increase the secretion of inhibiting cytokines in patients with malignant tumors, thus causing a Th1/Th2 imbalance and transient decreasing in the content of plasma immune globulin. Autologous transfusion has little impact and may even bring about some improvement oo postoperative immune function in patients with tumors. Therefore, cancer patients should receive active autologous transfusion during the perioperative period in place of allogeneic transfusion.

• 한국정보디스플레이학회:학술대회논문집
• /
• 2008.10a
• /
• pp.1520-1521
• /
• 2008

There are several ways to demonstrate white organic light emitting diodes (OLEDs) for displays and solid state lighting applications. Among these approaches are the stacked three primary or two complementary colors light-emitting layers, multiple-doped emissive layer, and excimer and exciplex emission [1-10]. We report on white phosphorescent excimer devices by using two light emitting materials based on platinum complexes. These devices showed a peak EQE of 15.7%, with an EQE of 14.5% (17 lm/W) at $500\;cd/m^2$, and a noticeable improvement in both the CIE coordinates (0.381, 0.401) and CRI (81). Devices with the structure ITO/PEDOT:PSS/TCTA (30 nm)/26 mCPy: 12% FPt (10 nm) /26 mCPy: 2% Pt-4 (15 nm)/BCP (40 nm)/CsF/Al [device 1], ITO/PEDOT:PSS/TCTA (30 nm)/26 mCPy: 2% Pt-4 (15 nm)/26 mCPy: 12% FPt (10 nm)/BCP (40 nm)/CsF/Al [device 2], and ITO/PEDOT:PSS/TCTA (30 nm)/26 mCPy: 2% Pt-4: 12% FPt (25 nm)/BCP (40 nm)/CsF/Al [device 3] were fabricated. In these cases, the emissive layer was either the double-layer of 26 mCPy:12% FPt and 15 nm 26 mCPy: 2% Pt-4, or the single layer of 26mCPy with simultaneous doping of Pt-4 and FPt. Device characterization indicates that the CIE coordinates/CRI of device 2 were (0.341, 0.394)/75, (0.295, 0.365)/70 at 5 V and 7 V, respectively. Significant change in EL spectra with the drive voltage was observed for device 2 indicating a shift in the carrier recombination zone, while relatively stable EL spectra was observed for device 1. This indicates a better charge trapping in Pt-4 doped layers [10]. On the other hand, device 3 having a single light-emitting layer (doped simultaneously) emitted a board spectrum combining emission from the Pt-4 monomer and FPt excimer. Moreover, excellent color stability independent of the drive voltage was observed in this case. The CIE coordinates/CRI at 4 V ($40\;cd/m^2$) and 7 V ($7100\;cd/m^2$) were (0.441, 0.421)/83 and (0.440, 0.427)/81, respectively. A balance in the EL spectra can be further obtained by lowering the doping ratio of FPt. In this regard, devices with FPt concentration of 8% (denoted as device 4) were fabricated and characterized. A shift in the CIE coordinates of device 4 from (0.441, 0.421) to (0.382, 0.401) was observed due to an increase in the emission intensity ratio of Pt-4 monomer to FPt excimer. It is worth noting that the CRI values remained above 80 for such device structure. Moreover, a noticeable stability in the EL spectra with respect to changing bias voltage was measured indicating a uniform region for exciton formation. A summary of device characteristics for all cases discussed above is shown in table 1. The forward light output in each case is approximately $500\;cd/m^2$. Other parameters listed are driving voltage (Bias), current density (J), external quantum efficiency (EQE), power efficiency (P.E.), luminous efficiency (cd/A), and CIE coordinates. To conclude, a highly efficient white phosphorescent excimer-based OLEDs made with two light-emitting platinum complexes and having a simple structure showed improved EL characteristics and color properties. The EQE of these devices at $500\;cd/m^2$ is 14.5% with a corresponding power efficiency of 17 lm/W, CIE coordinates of (0.382, 0.401), and CRI of 81.

• Korean Journal of Poultry Science
• /
• v.36 no.2
• /
• pp.157-163
• /
• 2009

This work was conducted to investigate the amount and major compositions of excreta from broilers. One hundred fifty 1-d-old male and female Ross broilers were used in this work and ten broilers were selected to measure excreta. Average amount of excreta per broiler was 85.4 g, and average moisture content was 76.9%. N, $P_2O_5$ and $K_2O$ in excreta were 1.19, 0.29 and 0.49%, respectively. pH, $BOD_5$, $COD_{mn}$, and SS were 7.80, 42,134 mg/L, 52,668 mg/L, and 106,669 mg/L, respectively. Pb in poultry excreta was 0.803 mg/kg and highly lowed compared with the standard contents (150 mg/kg). Cd was not founded in excreta. Hg was founded in excreta as 0.002 mg/kg, but it was low compared with the standard level (2 mg/kg).

• Korean Journal of Optics and Photonics
• /
• v.15 no.4
• /
• pp.361-368
• /
• 2004

We separated the multilayer structure of CD-R(compact disk-recordable) and investigated optimal spot marking conditions and physical and chemical transitions in response to various laser beam energh levels. Spot marking(80 ${\mu}{\textrm}{m}$ spot size) was produced on the surface of each layer using a Q-switched Nd:YAG laser between 27 mJ and 373mJ. By investigating resulting pit formation with Optical Microscopy(OM) and Optical Coherence Tomography(OCT), we analyzed the formation process of spot marking in the multilayer structure of different chemical composition. The localized heating of the substrate in the multilayer thin film caused the short temporal thermal expansion, and absorbed optical energy between reflective and dye interfaces melted dye and increased the volume. During the cooling phase, formation of pit and surrounding rim can be explained by three distinct processes; effect of surface tension, evaporation by spontaneous temperature increase due to laser energy, and mass flow from the recoil pressure. Our results shows that the spot marking formation process in the multilayer thin film is closely related to the layers' physical, chemical, and optical properties, such as surface tension, melt viscosity, layer thickness, and chemical composition.

• Journal of the Mineralogical Society of Korea
• /
• v.19 no.4 s.50
• /
• pp.259-264
• /
• 2006

This work carried out to analyze the physicochemical properties and investigate the assessment of heavy metal contamination of stream sediments of the Yudeung and the Gab streams in the Daejeon area. The pH of stream sediments of the Yudeung stream shows the range of weak acid and weak alkaline. Most of stream sediments contain about 80% sand grain and have low water and cation retention capacity. Hence the stream sediments are not suitable fur various plans to grow up. The analysis of heavy metals in the stream sediments shows that the concentration of Pb, Cr and Cd increases from upstream to down stream. It is likely that the trend has a relationship with the water. Contamination of stream water. The authors recommend that clay materials be replenished in the stream sediments to increase the self-purification capacity and to make the suitable condition for growing up of various plants, and that water quality of the stream which can Influence into the contamination of stream sediments be monitored.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.14
• /
• pp.97-109
• /
• 1973

1. The relatively lower prehardening temperature was more effective on increase of the hardiness of the intermediately hardy varieties than the higher one but either the extremely hardy or nonhardy varieties did not respond to the temperature as much as the intermediate types. 2. Five degree Centigrade was generally more favorable than $2^{\circ}C$ on hardening of the plants, especially when frozen at higher temperature for shorter duration. 3. It appears that photoperiod during prehardening and hardening did not play so important role as temperature on the hardiness. 4. The higher the soil moisture content, the higher the frost injury occurred. 5. Application of nitrogen increased markedly the hardiness and % DM of the plants. Percentage of dry matter of young seedlings might be used as an easy and rough criterion for evaluating hardiness since there was a highly significant regression of varietal frost injury on the %DM. 6. Four days appeared to be enough for hardening of plants although the plants increased gradually the hardiness as duration of hardening extended. Dehardening of the plants at relativity higher temperature took place rapidly within one to four days. 7. Under this controlled environment, freezing at about $-8^{\circ}C$ for 24 hours seemed the best for the purpose of evaluating the hardiness to low temperature. 8. It is believed that assessment of frost injury should be done at least one week after freezing. Some varieties showed strong ability to recover from the damage as recovery period was extended. 9. As a whole, Cd 80 and 83 were the most hardy and followed by Cappelle and Maris Otter. Four. winter oats varieties and Jufy I belonged to the intermediate type while the other three spring varieties were nonhardy at all. Peniarth was comparable with Maris Otter in hardiness. S 147 appeared the least hardy among the winter oats varieties. 10. It is evident that water-soluble carbohydrate content is associated with the hardiness to some extent but not primary factor involved in hardiness.

• Korean Journal of Environmental Agriculture
• /
• v.15 no.3
• /
• pp.282-288
• /
• 1996

Because of the high water content of the household garbage, it is difficult to compost it. Therefore, this study was conducted to investigate the possibility of using coal fly ash as humidity conditioner for the household garbage composting. The summarized results are as follows : 1. The maximum temperatures were $35^{\circ}C$ in spring, $47^{\circ}C$ in summer, and $43^{\circ}C$ in winter during the composting periods. 2. The mass was reduced to 70.5% after 60 days. The average volume reduced down to 74.7% after 60 days. 3. The seasonal variation of pH values showed a similar tendency and reached 8.5 after 60 days. 4. The water content was reduced at an early stage of composting and not much changed thereafter. It had 49.7% in spring, 33.9% in summer, and 56.5% in winter after 60 days. Ash contents were not much changed during the composting periods. 5. The contents of inorganic compounds were in the range of $0.06{\sim}4.03%$ $P_2O_5$, $0.01{\sim}2.87%$ CaO, $0.18{\sim}1.43%$ MgO, and $0.39{\sim}2.03%$ $K_2O$. Heavy metal contents were in the range of $ND{\sim}14.08$ Hg ${\mu}g/kg$, $ND{\sim}0.80$ Cd mg/kg, $4.99{\sim}28.95$ Cu mg/kg, $ND{\sim}242.62$ Cr mg/kg, $ND{\sim}20.24$ Pb mg/kg, and $ND{\sim}59.87$ Zn mg/kg.