• Journal of Society of Preventive Korean Medicine
• /
• v.5 no.1
• /
• pp.103-115
• /
• 2001

Background : Gamisamryungbaekchul-san(加味蔘?白朮散) is a herbal medicine which has been used for the traditional therapeutic agent of augmentation of the spleen and reinforcement of the Qi. Objective : This Study was performed to investigate the effect of Gamisamryungbaekchul-san on the tumor and immune response in the moose B16 melanoma tumor model. Materials and Methods : The tumor was induced by subcutaneous inoculation of B16BL6 melanoma cells in the shaved dorsal region of mice. Mice were orally administered with Gamisamryungbaekchul-san extract(26.3mg/mouse) for 14days after inoculation. For making examination of antitumor effect, the Increase of life span, Tumor growth inhibition rate, change of body weight were measured and evaluated. For the immune response increasing effect, the percentage of T lymphocyte and B Lymphocyte in the peripheral blood, the percentage of CD4+ T-cell, CD8+ T-cell and CD4+/CD8+ ratio in the peripheral blood and spleen, interleukin-2 productivity were measured and evaluated. Results : Gamisamryungbaekchul-san showed 16.59% increase of life span, 31.64% tumor growth inhibition rate and increase of body weight. Gamisamryungbaekchul-san increased the percentage of T lymphocyte in the peripheral blood, CD4+ T cell percentage of peripheral blood and spleen, and Interleukin-2 productivity as compared with the Control group. Whereas Gamisamryungbaekchul-san had no effect on the percentage of B lymphocyte in the peripheral blood, the percentage of CD8+ T cell, CD4+/CD8+ T-cell ratio in both of peripheral blood and spleen as compared with the Control group. Conclusion : This study shows that Gamisamryungbaekchul-san has anti-tumor effects and immunoregulatory effects on the B16 melanoma tumor model. It is suggested that Gamisarmyungbaekchul-san could be a useful immunomodulator and anti-tumor agent.

• Pediatric Gastroenterology, Hepatology & Nutrition
• /
• v.17 no.3
• /
• pp.147-154
• /
• 2014

Purpose: Gender differences in paediatric patients with inflammatory bowel disease (IBD) are frequently reported as a secondary outcome and the results are divergent. To assess gender differences by analysing data collected within the Swiss IBD cohort study database since 2008, related to children with IBD, using the Montreal classification for a systematic approach. Methods: Data on gender, age, anthropometrics, disease location at diagnosis, disease behaviour, and therapy of 196 patients, 105 with Crohn's disease (CD) and 91 with ulcerative or indeterminate colitis (UC/IC) were retrieved and analysed. Results: The crude gender ratio (male : female) of patients with CD diagnosed at <10 years of age was 2.57, the adjusted ratio was 2.42, and in patients with UC/IC it was 0.68 and 0.64 respectively. The non-adjusted gender ratio of patients diagnosed at ${\geq}10$ years was 1.58 for CD and 0.88 for UC/IC. Boys with UC/IC diagnosed <10 years of age had a longer diagnostic delay, and in girls diagnosed with UC/IC >10 years a more important use of azathioprine was observed. No other gender difference was found after analysis of age, disease location and behaviour at diagnosis, duration of disease, familial occurrence of IBD, prevalence of extra-intestinal manifestations, complications, and requirement for surgery. Conclusion: CD in children <10 years affects predominantly boys with a sex ratio of 2.57; the impact of sex-hormones on the development of CD in pre-pubertal male patients should be investigated.

• Pediatric Gastroenterology, Hepatology & Nutrition
• /
• v.20 no.1
• /
• pp.41-46
• /
• 2017

Purpose: Vitamin D deficiency is common in Crohn disease (CD). The aim of the study was to examine the prevalence of vitamin D deficiency and evaluate the association between vitamin D status and growth outcome in Korean pediatric CD patients. Methods: In this retrospective study, 17 children younger than 18 years old diagnosed with CD were enrolled and their serum 25-hydroxy vitamin D (25[OH]D) was checked between 2011 and 2015. We categorized the patients into two groups, Group 1 and Group 2. Group 1 included patients with serum 25(OH)D levels below 10 ng/mL, and Group 2 was for patients with a 25(OH)D serum levels between 10 ng/mL and 30 ng/mL. The z-scores for height (Htz), weight (Wtz), and body mass index (BMIz) were measured at baseline, 6 months, and 12 months. Results: The mean serum 25(OH)D levels of the total 65 CD patients and 17 enrolled patients were $15.64{\pm}6.9ng/mL$ and $13.1{\pm}5.1ng/mL$, respectively. There was no correlation at the beginning of the study between vitamin D level and growth parameters (Htz, Wtz, BMIz) or other variables including laboratory data and Pediatric Crohn Disease Activity Index. The Htz, Wtz, and BMIz in Group 1 showed no significant improvement at 6 months and 12 months follow-up. In Group 2, Wtz and BMIz showed significant improvements sustained until 12 months of follow-up. Htz showed no significant improvement at 6 months but there was significant improvement at 12 months. Conclusion: It seems that baseline vitamin D status affects growth outcome in pediatric CD.

• Pediatric Gastroenterology, Hepatology & Nutrition
• /
• v.24 no.2
• /
• pp.197-206
• /
• 2021

Purpose: Celiac disease (CD) is a common autoimmune disease with extra-intestinal manifestations, including neurological disorders. There are few reports to assess various factors in increasing the chances of developing neurological disorders in CD, so we designed this study. Methods: All patients with CD at any age who had been referred to the Celiac Clinic were evaluated for neurological problems. CD was defined as IgA anti-transglutaminase antibodies (anti-tTG) of 18 IU/mL or higher in serology and Marsh type I or more severe in histopathological evaluation. Logistic regression analysis was used to evaluate the impact of various independent variables on the neurological manifestations. Results: A total of 540 patients enrolled in this study. A 360 (66.7%) of patients were children. A 64.8% and 35.2% were female and male, respectively. Overall, 34.1% of patients had neurological manifestation, including headache, neuropathy, epilepsy, and ataxia. The odds of developing neurological manifestations in children were significantly lower than in adults (odds ratio [OR], 0.66; 95% confidence interval [CI], 0.45-0.96; p=0.03) and in patients with gastrointestinal (GI) symptoms significantly higher than in the group without GI manifestations (OR, 1.77; 95% CI, 1.18-2.63; p=0.005). Other variables, including Marsh classification (OR, 0.44; 95% CI, 0.18-1.11; p=0.08) and anti-tTG levels (OR, 1.00; 95% CI, 0.999-1.001; p=0.59) did not significantly increase the chances of developing neurological disorders. Conclusion: Our study showed that increasing age and the presence of GI symptoms, but not serological and histological findings, could increase the chances of developing neurological diseases in CD patients.

• The Korean Journal of Ecology
• /
• v.25 no.6
• /
• pp.383-389
• /
• 2002

Pb and Cd concentrations and reproductive progress of feral pigeons were investigated in urban (Seoul) and industrial complex (Ansan) areas from November 2000 to May 2001. Results of the Pb analysis for the feral pigeons from the Ansan industrial complex (egg contents: 1.13 $\mu$g/wet g, bones of adult: 10.5 $\mu$g/wet g) and Seoul (1.64 $\mu$g/wet g, 29.5 $\mu$g/wet g, respectively) indicated that the Pb level of eggs and bones of adults were significantly different between the two colonies (p<0.05). Cd concentrations in liver and kidney of adult pigeons were also significantly different between the Ansan (liver: 0.14 $\mu\textrm{g}$/wet g, kidney: 0.43 $\mu$g/wet g) and Seoul (liver. 0.24 $\mu$g/wet g, kidney: 1.05 $\mu$g/wet g) colonies. (p<0.05). However, egg size and thickness, incubation period and nestling growth rates did not differ between the study areas. Also, clutch size, number of young hatched per nest and number of young fledglings per nest did not significantly different in the noted areas. Considering the lead and cadmium concentrations of pigeons, these were not as high as those considered as results in toxic effects in other species, and the biological significance from these level differences is uncertain.

• Asian-Australasian Journal of Animal Sciences
• /
• v.14 no.2
• /
• pp.268-275
• /
• 2001

The effects of homogenization conditions and $\beta$-cyclodextrin ($\beta$-CD) on cholesterol removal of Mozzarella cheese were examined. The homogenization pressure influenced markedly on the cholesterol removal in milk and, 75.64% of cholesterol, the highest rate, was removed at $70.0kg/cm^2$. In addition, an increase in temperature resulted in an increase of cholesterol removal in the range of 71.75 to 78.22%. Among different concentrations of $\beta$-CD addition, 1.0% showed 78.21% of cholesterol removal. Therefore, cholesterol-reduced Mozzarella cheese was made by cheese milk treated with 70 $70.0kg/cm^2$ homogenization at $70^{\circ}C$ and 1% $\beta$-CD addition for a subsequent study. The cholesterol reduction of cholesterol-reduced Mozzarella cheese was 63.92%. Meltability, stretchability and oiling-off in cholesterol-reduced cheese were significantly lower than those in control. Hardness, gumminess and chewiness were significantly reduced, while cohesiveness and elasticity increased. Appearance and flavor of the cheese were superior, but texture inferior to the control.

• The Korean Journal of Food And Nutrition
• /
• v.15 no.1
• /
• pp.64-69
• /
• 2002

To know a degree of pollution in agricultural products cultivated around 4 unworked mines located in Chollanam-Do, we investigated a concentration of heavy metal and trace metal to provide the basic data for its residual limits. 28 samples of seven kinds of agricultural products(lettuce, onion, potato, radish, pepper, pumpkin, bean) collected from 4 unworked mines located in Chollanam-Do in 2001 were analyzed by Mercury Analyzer for mercury and Atomic Absorption spectrophotometer for Pb, As, Cd, Cu, Mn, and Zn. Hg contents were detected N.D. ∼trace level(0.01 ppm and less) and As content were detected N.D. ∼ 0.029 lpm but most of same)leE were not detected. Cd contents were detected N.D∼0.124 ppm. The results of Hg, As, Pb and Cd content showed that for all the 7 species of agricultural products studied, none have accumulated levels dangerous enough to Pose health problems. The average contents of Cu were 3.070 ∼ 7.825 ppm in bean, the Mn were 3.688 ∼23.935 ppm in lettuce ailed the Zn were 5.690 ∼21.171 ppm in bean, respectively.

• Journal of Information Management
• /
• v.26 no.4
• /
• pp.64-99
• /
• 1995

As advanced information technology has been developing rapidly, libraries are moving from an ownership-centered information service toward information access service. Consequently, integrated automation systems of information centers that give not only cataloging information of their own collections but also various electronic information have appeared. In this study, a model of integrated automation system of an information center is suggested in terms of information types and cost, by taking case studies of some domestic and foreign libraries. Furthermore, the MIPOS Information & Resource Center System of POSCO Group is introduced as a good example of integrated automation systems in Korea.

• Microbiology and Biotechnology Letters
• /
• v.24 no.5
• /
• pp.630-635
• /
• 1996

For a survey of the occurrence of aflatoxin M$_{1}$ (AFM$_{1}$) in domestic cow's milk, we developed an enzyme-linked immunosorbent assay (ELISA) system, and quantitated the toxin in cow's milk. In order to produce specific antibodies AFM, conjugated to bovine serum albumin (AFM$_{1}$-BSA) and Freund's adjuvant were immunized subcutaneously to rabbits. By use of the antiserum showing the highest titer and AFB$_{1}$-HRP conjugate, we established a competitive direct ELISA (cdELISA) for AFM$_{1}$, whose detection limit was 0.003 ppb. The cross-reactivities of the antiserum against aflatoxin M$_{1}$ M$_{2}$, B$_{1}$, B$_{2}$, G$_{1}$, G$_{2}$, B$_{2a}$, and G$_{2a}$, were 100, 29.9, 25.0, 2.7, 13.0, 0.65, 0, and 0%, respectively. When the cdELISA was applied to the cow's milk spiked with AFM$_{1}$ and followed by cleanup with C$_{18}$ cartridge, the mean recovery of the assay was 104% (mean of CV, 6.4%) in the final concentration of 0.01-1 ppb (10-1, 000 ppt). When cow's milk samples gathered from markets and farms were assayed by the cdELISA, the mean concentration and SD of AFM$_{1}$ was 80.4 $\pm$ 55.0 ppt (n=64; range, 5.6-280 ppt).

• Proceedings of the Acoustical Society of Korea Conference
• /
• autumn
• /
• pp.49-52
• /
• 2004

본 논문은 연속 밀도 함수를 갖는 HMM별 한국어 연속 음성 인식에 관한 연구이다. 여기서 우리는 밀도 함수가 2개에서 44개까지 갖는 연속 HMM모델에서 가장 효율적인 연속 음성 인식을 위한 방법을 제시한다. 음성 모델은 36개로 구성한 기본음소를 사용한 CI-Model과 3,000개로 구성한 확장음소를 사용한 CD-Model을 사용하였고, 언어 모델은 N-gram을 이용하여 처리하였다. 이 방법을 사용하여 500개의 문장과 6,486 개의 단어에 대하여 화자 독립으로 CI Model에서 최고 $94.4\%$의 단어 인식률과 $64.6\%$의 문장 인식률을 얻었고, CD Model에서는 $98.2\%$의 단어 인식률과 $73.6\%$의 문장인식률을 안정적으로 얻었다.