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Characterization of $\beta$-Ketoadipate Pathway from Multi-Drug Resistance Bacterium, Acinetobacter baumannii DU202 by Proteomic Approach

  • Park, Soon-Ho;Kim, Jae-Woo;Yun, Sung-Ho;Leem, Sun-Hee;Kahng, Hyung-Yeel;Kim, Seung-Il
    • Journal of Microbiology
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    • v.44 no.6
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    • pp.632-640
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    • 2006
  • In this study, the biodegradative activities of monocyclic aromatic compounds were determined from the multi-drug resistant (MDR) Acinetobacter baumannii, which were studied in the form of clinical isolates from a hospital in Korea. These bacteria were capable of biodegrading monocyclic aromatic compounds, such as benzoate and p-hydroxybenzoate. In order to determine which pathways are available for biodegradation in these stains, we conducted proteome analyses of benzoate, and p-hydroxybenzoate-cultured A. baumannii DU202, using 2-DE/MS analysis. As genome DB of A. baumannii was not yet available, MS/MS analysis or de novo sequencing methods were employed in the identification of induced proteins. Benzoate branch enzymes [catechol 1,2-dioxygenase (CatA) and benzoate dioxygenase $\alpha$ subunit (BenA)] of the $\beta$-ketoadipate pathway were identified under benzoate culture condition and p-hydroxybenzoate branch enzymes [protocatechuate 3,4-dioxygenas $\alpha$ subunit (PcaG) and 3-carboxy-cis,cis-muconate cycloisomerase (PcaR)] of the $\beta$-ketoadipate pathway were identified under p-hydroxybenzoate culture condition, respectively, thereby suggesting that strain DU202 utilized the $\beta$-ketoadipate pathway for the biodegradation of monocyclic aromatic compounds. The sequence analysis of two purified dioxygenases (CatA and PcaGH) indicated that CatA is closely associated with the CatA of Acinetobacter radiresistance, but PcaGH is only moderately associated with the PcaGH of Acinetobacter sp. ADPI. Interestingly, the fused form of PcaD and PcaC, carboxymuconolactone decarboxylase (PcaCD), was detected on benzoate-cultured A. baumannii DU202. These results indicate that A. baumannii DU202 exploits a different $\beta$-ketoadipate pathway from other Acinetobacter species.

Studies on the Chemical Compositions and Distributions of Ambient Sumicron Aerosols (Submicron 부유분진의 화학적 조성 및 분포에 관한 연구)

  • 황인조;김동술
    • Journal of Korean Society for Atmospheric Environment
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    • v.14 no.1
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    • pp.11-23
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    • 1998
  • The purpose of this study was to survey chemical distribution of inorganic elements and ions in the submicron particles, to characterize qualitatively emitting sources by factor analysis, and finally to reveal existing patterns in terms of chemical compounds by a stepwise multiple regression analysis. Total of 141 samples were collected by a cascade impactor from 1989 to1996. Fifteen chemical species (Al, Ba, Cd, K, Pb, Cu, Fe, Ni, $Cl^-, NO_3^-, SO_4^{2-}, K^+, Mg^{2+}, Ca^{2+}, and Na^+$) were characterized by AAS and IC. The study showed that average seasonal levels of submicron particulate matters $(d_p<0.43 \mum)$ were 18.7 $\mug/m^3$ in spring, 15.5 $\mug/m^3$ in summer, 15.7 $\mug/m^3$ in fall, and 24.5 $\mug/m^3$ in winter, respectively. All of the anion concentrations in the particle were highest in the winter season. By applying a factor analysis, 5 source patterns were qualitatively obtained, such as sulfate related source, nitrate related source, oil burning source, calcium related source, and coal combustion source. Finally, when applying a stepwise multiple regression analysis, the results clearly showed that $Na^+ and Ca^{2+}, K^+ and Ca^{2+}, NO_3^-$ and relative humidity, $Cl^-$ and ambient temperature, $Ca^{2+} and Cl^-, Mg^{2+} and SO_4^{2-}, Na^+ and NO_3^-, and Ca^{2+} and NO_3^-$, respectively, are negatively contributed to each other. As a result of those statistical analysis, we could suggest that some chemical compounds in the submicron particles such as$NaNO_3, MgSO_4, Ca(NO_3)_2, and CaCl_2$ may not exist on the filter as final composing products; however, other compounds may possibly exist in the form of $Mg(NO_3)_2, CaSO_4, Na_2SO_4, K_2SO_4, MgCl_2, NaCl, and KCl$. Thus, it must be necessary to identify differences between the results of above statistical analysis and of the real world by laboratory experiments.

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Effects of non-genetically modified organism wheat-based diets on growth performance, nutrient digestibility, blood profile, and meat quality in grower-finisher pigs

  • Wang, Huan;Kim, Dae Won;Yoo, Jong Sang;Kim, In Ho
    • Korean Journal of Agricultural Science
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    • v.46 no.4
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    • pp.705-713
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    • 2019
  • This study was conducted to determine the effects of dietary non-genetically modified organism (non-GMO) wheat-based diets on the growth performance, nutrient digestibility, blood profile and meat quality of grower-finisher pigs. A total of 70 [(Landrace × Yorkshire) × Duroc] growing pigs with an initial body weight of 26.15 ± 1.57 kg were used in a 112 day trial. The dietary treatments were as follows: (I) CD, corn-based diet and (II) non-GMO WD, a non-genetically modified organism wheat-based diet. Each treatment consisted of 7 replicate pens with 5 pigs per pen. In the current study, the pigs fed the corn-based diet had a higher body weight than the pigs fed the non-GMO wheat-based diet at day 21 and day 77 (p < 0.05). There was a significant difference in the average daily gain (ADG) during the first 21 days (p < 0.05). The non-GMO wheat-based diet had no effect on nutrient digestibility. In addition, the non-GMO wheat-based diet had no effect on the blood profile except for blood urea nitrogen (BUN) at d 21. In conclusion, the non-GMO wheat-based diet only had a slight effect on the growth performance of growing pigs but had no significant impact on the nutrient digestibility, fecal score, blood profile and meat quality of the pigs during the grower-finisher period.

사용자 인터페이스 디자인과 개발 User Interface Design and development

  • 천진향
    • Archives of design research
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    • v.13 no.1
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    • pp.29-38
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    • 2000
  • Many computer system; (or program;) have been desigoc>d without taking their users needs into accOlmt To provide a practical and comprehensive introduction to the design and development of user interfaces, desigrer (or developer) is suggested to analyze and evaluate requirements from the view point of users and to design more effective program;. The su~ect of this thesis is to analyze and criticize tre details of Wonder-land, that is a demo multimedia ill-ROM title, in respect to User Interface Design and Development. By applying the coocepts, teclmiques and ideas to a real life study and analyzing and criticizing it from the view point of users, desigrer lets the program revised and developed by users. For User Interface Design and Development purposes this tresis analyzes and criticize the characteristics of the program already made, the current user interface design, mental models and users learning about the program. As a result it is founded that the program requires to be analyzing and criticizing continuously from a cognitive point of view to design and develop it successfully.

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DAQ System을 활용한 초지기 맥동현상의 개선

  • 이영준;김도환;서동준;고두석;이복진
    • Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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    • 2001.04a
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    • pp.81-81
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    • 2001
  • 초지 공정은 wire, roll, pump등의 회전체로 이루어진 공정이 거의 대부분이기 때문에 주기적으로 변동하는 성분들이 많고, QCS(Quality Control System) 및 DCS(Distributed Co n ntrol System)상에 서 의 자동 Control Logic에 의 한 불안정 (Fluctuation)요소도 내 재 되 어 있 어 이에 대한 분석이 필요하다. 이러한 주기적인 변동은 제품의 여러 물성(평량, 두께, 광택 도등) 에 영향을 미치게 되어 품질의 균일성이나 프로파일에 영향을 미치게 된다. 따라서 공정상이나 품질에서 주기적으로 변동하는 성분을 추출하여 공정 각 성분과 연관을 시키변 품질에 나타나는 주기적 성분의 요인이 어느 부분에서 기인하는 지를 직접적으로 찾아낼 수 있고 이의 개선을 통해 품질의 균일성 및 프로파일 향상을 꾀할 수 있다. 본 연구에서는 Air padded type 헤드박스 초지기에서 DAQ(Data Acquisition) System 을 이용하여 각 공정별로 MD(Machine Direction)방향으로의 변동을 측정하고 제품의 물성 변동과 비교하였다. DAQ System은 각 공정상의 전기적 신호를 높은 주파수 영역까지 다채널로 동시에 받 아 들일 수 있는 장치로 이 시스템을 활용하여 공정상의 Machine chest 농도, Stock box 유량 및 레벨, Fan pump RPM, Cleaner압력, Screen압력, H!B압력 및 레벨, Silo level등 공 정요소의 변동상태와 상호영향을 분석하였다. 이러한 공정 각 요소의 변동과 MD 방향의 제품 물성 값(Tapio, Paper Variation Analyzer에 대한 비교 분석결과 제품 평량에서 주기적인 변동성분을 확인할 수 있었고 이중 큰 비 중을 차지하는 것이 fνB 레벨 변동주기와 일치하는 것을 찾아낼 수 있었다. 이에 근거하여 D DCS system의 control 요소인 튜닝 parameter(P,I 값)들을 미 세 조정 하여 제 품의 MD방향 평량 변동 진폭을 기존 수준의 30% 수준으로 감소시킬 수 있었다. 그 결과 COV(표준편차/ 평량)값을 0.8-1.2%에서 0.6-0.8%수준으로 낮출 수 있었고 이에 따라 CD (Cross Direction) 방향의 제품 Profile도 함께 개선되는 효과를 거둘 수 있었다.

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Tunisian phosphogypsum tailings: Assessment of leaching behavior for an integrated management approach

  • Zmemla, Raja;Sdiri, Ali;Naifar, Ikram;Benjdidia, Mounir;Elleuch, Boubaker
    • Environmental Engineering Research
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    • v.25 no.3
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    • pp.345-355
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    • 2020
  • This study has been carried out to evaluate the leaching behavior of Tunisian phosphogypsum (PG) tailings in Skhira city (southern Tunisia). Two PG samples, including old and freshly deposited samples, were characterized in terms of physical, geotechnical, mechanical, chemical and mineralogical properties. Special attention was paid to their leaching behavior when subjected to standard leaching tests. Our results indicated that both samples are mainly composed of more than 31.85% CaO and 31.4% SO3, indicating the predominance of gypsum. This was further confirmed by XRD patterns that revealed the presence of characteristic reflections of gypsum, brushite, quartz and Maladrite. Compressive strength after 90 d exceeded 769 kPa, but still lower than that of natural sand (1,800 kPa). Leaching test was proposed as an appropriate method to determine the released contaminants from PG. The obtained results showed that Fluorine and Phosphorus are the most released elements from PG with 40 and 30%, respectively. The released Se, Cd, and Zn were the only trace elements that exceeded the threshold limits. It seemed that leached element concentrations were independent aging or particle size of the PG. Based on the assessment of leaching behavior, an integrated management approach of the PG deposits was proposed.

A Study on the Utilization of Waste Tire/Waste Moter Oil Pyrolytic Residue for Asphalt (폐타이어/폐윤활유 열분해 잔류물의 아스팔트 활용기술)

  • 김상국;손성근;김동찬
    • Resources Recycling
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    • v.4 no.4
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    • pp.16-21
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    • 1995
  • When waste t~re/~vastmz otor oil is pyrolyzed. most of them hecome gaseous produds. and thc remaining onc, whascwelght is ahout in% oi the waste Ore, is pyrolyced residue mnstly composcd oi ca~bnn black A rescsrcll was canicrl nut loutilize lhe pyralyred residue of waste tnelwuste lnotol 011 us retnin~cing agent of asphall concrete, bescd on iolelg~r lesearchrepurl. This shows thal the properlies ol asphall concrele ~nclud~cd~ugl ah~l~tyre, sistance to Tear. temperature-v~scusilysusceptil,ilily u e g reatly improved when lhe pellellrcd hrln aI carlmn hlack usmg petroleum o ~als a hinder Iar ihe pellels isused with asphalt. The surface of the pyralyred resirh~ei s covned by ocl film and thla lnakes good comllatibllity with asphallIn order lo ulilk pyrolyzed residue as a reinforcing agenl oi lhe itsphalt concrete, various tests such as Marshnll tcsi, wheeltracking, and revelhng test has been carried out a1 KLER, Ko~ea I-lighway Coo~poration, and TCMO. Tcst lcsults satirry KSslandard, show "npmvements an the dynam~cs tab~l~lzym, d incrcase reslslance to wear at cold telnpelatule Invrsligadon wascarlied oul to sludg the possibility of soil pallul~on when pyrolyzed residue is used as a tzmioicing agenl. E~pcrimentalresulls show the rcsidue contained in thc asphall docs not cause cnv~ranma~lparlo blems.e cnv~ranma~lparlo blems.

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A Study of Long Term Recording Reserved Type Material by Using Glass Micro-structure (유리의 미세구조를 이용한 장기보존형 기록재료에 관한 연구)

  • Lee, Kang-Taek;Yoon, Duk-Ki;Chin, Hyun-Ju;Choi, Kwang-Hoon;Lee, Kyu-Ho;Kim, Hyun-Gyu;Ryu, Bong-Ki
    • Korean Journal of Materials Research
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    • v.16 no.12
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    • pp.777-781
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    • 2006
  • Recently, there are a lot of study to alternate polycarbonate which is being used as storage material in CD, DVD. In this study, we alternated polycarbonate with glass. We observed the change of shape in a surface of the glass which was focused by Nd:YAG Laser. The change of shape and property was studied by thermal mechanical analysis (TMA), UV-Vis spectrometer, AFM and SEM. According to Laser power and quantity of additives, the Bump's size and shape are showed differently. In high energy, the Bump will be transformed into Pit. And also according to CTE, $T_d$ and absorption ratio of glass, difference between Bump and Pit is confirmed. From these investigation, we could control that the minimum size of bump which is more useful shape than pit's is about 1.3 $\mu$m, H 70 nm, and it is near same the spot size.

Interleukin-2/antibody complex expanding Foxp3+ regulatory T cells exacerbates Th2-mediated allergic airway inflammation

  • Hong, Sung-Wook;O, Eunju;Lee, Jun Young;Yi, Jaeu;Cho, Kyungjin;Kim, Juhee;Kim, Daeun;Surh, Charles D.;Kim, Kwang Soon
    • BMB Reports
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    • v.52 no.4
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    • pp.283-288
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    • 2019
  • $Foxp3^+$ regulatory $CD4^+$ T (Treg) cells play an essential role in preventing overt immune responses against self and innocuous foreign antigens. Selective expansion of endogenous Treg cells in response to the administration of interleukin (IL)-2/antibody complex, such as the IL-2/JES6-1 complex (IL-2C) in mice, is considered an attractive therapeutic approach to various immune disorders. Here, we investigated the therapeutic potential of IL-2C in allergic airway inflammation models. IL-2C treatment ameliorated Th17-mediated airway inflammation; however, unexpectedly, IL-2C treatment exacerbated Th2-mediated allergic airway inflammation by inducing the selective expansion of Th2 cells and type-2 innate lymphoid cells. We also found that IL-2 signaling is required for the expansion of Th2 cells in lymphoproliferative disease caused by Treg cell depletion. Our data suggest that IL-2C is selectively applicable to the treatment of allergic airway diseases depending on the characteristics of airway inflammation.

Processing Method and Feed Value of Food Wastes as Swine Feed Resources (음식폐기물의 양돈사료 자원화를 위한 처리공정 및 사료가치 평가)

  • Jung, Woo-Jin;Lee, Jeong-Chae;Kim, Tae-Hwan;Lim, Kye-Taek
    • Korean Journal of Environmental Agriculture
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    • v.19 no.1
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    • pp.44-50
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    • 2000
  • This study was carried out to get the basic data on the efficient processing method of food wastes for utilizing as the resources of swine feeds composition, mineral and toxic elements of food waste were estimated in relation to the steps of processing and fermentation with MS (Miraculous soil-bacteria) microorganism complex. The chemical composition of food wastes was largely varied according to disposing sites, collection time and season. Offensive odor was reduced by anaerobic fermentation with MS microorganism complex. Food wastes fermented with MS microorganism complex have high contents in crude protein (24.1% D.M) and crude fat (12.9% D.M). Mineral composition was to be relatively well balanced compared to other plant or animal feed resources. Particularly the content of sodium was slightly higher than that of grains or agricultural byproducts. In food wastes fermented with MS microorganism complex, heavy metals such as Hg, As or F were not detected. Pb or Cd were detected in small quantities but their levels were below the dose s-permitted feeding standard of permitted dose in feeding standard. These results indicated that food wastes could be efficiently used for the resources of swine feeds through proper processing and fermentation.

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