• The Korean Journal of Physiology and Pharmacology
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• v.12 no.2
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• pp.59-64
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• 2008

In our previous study, we found that spermine and putrescine inhibited spontaneous and acetylcholine (ACh)-induced contractions of guinea-pig stomach via inhibition of L-type voltage- dependent calcium current ($VDCC_L$). In this study, we also studied the effect of spermidine on mechanical contractions and calcium channel current ($I_{Ba}$), and then compared its effects to those by spermine and putrescine. Spermidine inhibited spontaneous contraction of the gastric smooth muscle in a concentration-dependent manner ($IC_{50}=1.1{\pm}0.11mM$). Relationship between inhibition of contraction and calcium current by spermidine was studied using 50 mM high $K^+$-induced contraction: Spermidine (5 mM) significantly reduced high $K^+$ (50 mM)-induced contraction to 37${\pm}$4.7% of the control (p<0.05), and inhibitory effect of spermidine on $I_{Ba}$ was also observed at a wide range of test potential in current/voltage (I/V) relationship. Pre- and post-application of spermidine (5 mM) also significantly inhibited carbachol (CCh) and ACh-induced initial and phasic contractions. Finally, caffeine (10 mM)-induced contraction which is activated by $Ca^{2+}$-induced $Ca^{2+}$ release (CICR), was also inhibited by pretreatment of spermidine (5 mM). These findings suggest that spermidine inhibits spontaneous and CCh-induced contraction via inhibition of $VDCC_L$ and $Ca^{2+}$ releasing mechanism in guinea-pig stomach.

• Journal of KIISE:Information Networking
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• v.33 no.4
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• pp.310-323
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• 2006

In this paper, we describe a secure cluster-routing protocol based on a multi-layer scheme in ad hoc networks. We propose efficient protocols, Authentication based on Multi-layer Clustering for Ad hoc Networks (AMCAN), for detailed security threats against ad hoc routing protocols using the selection of the cluster head (CH) and control cluster head (CCH) using a modification of cluster-based routing ARCH and DMAC. This protocol provides scalability of Shadow Key using threshold authentication scheme in ad hoc networks. The proposed protocol comprises an end-to-end authentication protocol that relies on mutual trust between nodes in other clusters. This scheme takes advantage of Shadow Key using threshold authentication key configuration in large ad hoc networks. In experiments, we show security threats against multilayer routing scheme, thereby successfully including, establishment of secure channels, the detection of reply attacks, mutual end-to-end authentication, prevention of node identity fabrication, and the secure distribution of provisional session keys using threshold key configuration.

• Molecules and Cells
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• v.20 no.3
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• pp.435-441
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• 2005

Classical transient receptor potential channels (TRPCs) are thought to be candidates for the nonselective cation channels (NSCCs) involved in pacemaker activity and its neuromodulation in murine stomach smooth muscle. We aimed to determine the role of TRPC4 in the formation of NSCCs and in the generation of slow waves. At a holding potential of -60 mV, $50{\mu}M$ carbachol (CCh) induced $I_{NSCC}$ of amplitude [$500.8{\pm}161.8pA$ (n = 8)] at -60 mV in mouse gastric smooth muscle cells. We investigated the effects of commercially available antibodies to TRPC4 on recombinant TRPC4 expressed in HEK cells and CCh-induced NSCCs in gastric smooth muscle cells. TRPC4 currents in HEK cells were reduced from $1525.6{\pm}414.4pA$ (n = 8) to $146.4{\pm}83.3pA$ (n = 10) by anti-TRPC4 antibody and $I_{NSCC}$ amplitudes were reduced from $230.9{\pm}36.3pA$ (n = 15) to $49.8{\pm}11.8pA$ (n = 9). Furthermore, $I_{NSCC}$ in the gastric smooth muscle cells of TRPC4 knockout mice was only $34.4{\pm}10.4pA$ (n = 8) at -60 mV. However, slow waves were still present in the knockout mice. Our data suggest that TRPC4 is an essential component of the NSCC activated by muscarinic stimulation in the murine stomach.

• The Korean Journal of Physiology and Pharmacology
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• v.26 no.3
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• pp.219-225
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• 2022

Glucagon like peptide-1 (GLP-1) released from enteroendocine L-cells in the intestine has incretin effects due to its ability to amplify glucose-dependent insulin secretion. Promotion of an endogenous release of GLP-1 is one of therapeutic targets for type 2 diabetes mellitus. Although the secretion of GLP-1 in response to nutrient or neural stimuli can be triggered by cytosolic Ca²⁺ elevation, the stimulus-secretion pathway is not completely understood yet. Therefore, the aim of this study was to investigate the role of reverse Na⁺/Ca²⁺ exchanger (rNCX) in Ca²⁺ entry induced by muscarinic stimulation in NCI-H716 cells, a human enteroendocrine GLP-1 secreting cell line. Intracellular Ca²⁺ was repetitively oscillated by the perfusion of carbamylcholine (CCh), a muscarinic agonist. The oscillation of cytosolic Ca²⁺ was ceased by substituting extracellular Na⁺ with Li⁺ or NMG⁺. KB-R7943, a specific rNCX blocker, completely diminished CCh-induced cytosolic Ca²⁺ oscillation. Type 1 Na⁺/Ca²⁺ exchanger (NCX₁) proteins were expressed in NCI-H716 cells. These results suggest that rNCX might play a crucial role in Ca²⁺ entry induced by cholinergic stimulation in NCI-H716 cells, a GLP-1 secreting cell line.

• The Journal of Information Technology and Database
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• v.1 no.1
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• pp.63-81
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• 1994

The trigger mechanism in active object-oriented database systems is known to be a good tool for describing user-defined integrity constraints. It cannot adequately support, however, certain integrity constrains specified on the objects in class composition hierarchy. Those are the cases where the constraints must be maintained in the forward direction along the composition hierarchy as well as in the backward direction We call theses kinds of problems "backward propagation problem" and investigate several ways to resolve them using the currently available techniques. Based on them, a new constructor, called CONSTRAIN $T^{cch}$, is proposed. The constructor can be realized with enhanced facilities for active OODBMS which we recommend in this paper.d facilities for active OODBMS which we recommend in this paper.r.

• Proceedings of the KIEE Conference
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• 1995.11a
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• pp.414-416
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• 1995

We have studied the effect of the mesogenic core of nematic liquid crystals (NLCs) for polar (out-of-plane tilt) anchoring strength on rubbed polyimide (PI) films. The order of polar anchoring strength for NLCs on rubbed PI films is 5CB > PCH5 > CCH5. This order is the same for all rubbing strengths investigated. From these results, we suggest that the polar anchoring strength strongly depends on the polarizability of the NLCs.

• Proceedings of the Korea Water Resources Association Conference
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• 2011.05a
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• pp.144-149
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• 2011

합류부에서의 수공구조물의 설치는 수리학적으로는 매우 불리하며, 최근 심각해지고 있는 국지성 집중호우로 인한 과중한 유량이 합류부에 집적되어 홍수위를 상승시킴으로써 하천 범람 피해의 주원인이 된다. 본 연구에서는 1차원 HEC-RAS 및 2차원 수치모형 CCH2D을 이용하여 합류부에서의 교각설치에 따른 하천흐름 변화를 분석하고 각 모형의 성과를 비교 검토한다. 또한 2차원 모형인 CCHE2D 모형의 원형교각 배치에 대한 단점을 보완하고자 2차원 수리해석 모형인 FLUMEN모형과 함께 비교 분석하여 실제 교각의 배치, 형상 및 제원이 고려된 격자망을 이용하여 합류부 주변에 위치한 교량에 의한 흐름 유동특성을 평가한다.

• Korean Journal of Animal Reproduction
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• v.18 no.3
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• pp.175-182
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• 1994

Rat $\beta$-casein 유전자와 사람 성장호르몬 (hGH) 유전자의 융합유전자를 생쥐 수정란의 웅성전핵에 미세주입하여 형질전환생쥐 (transgenic mouse) 6계통을 확립하였다. 이들로부터 사람성장호르몬(hGH) 유전자의 발현 여부를 조사한 결과, 6계통중 4계통의 생쥐 유즙에서 hGH가 2~900 ng/ml 수준으로 발현되고 있었으며, 혈중에서는 hGH가 검출되지 않았다. 따라서 이들 형질전환생쥐에서 사람성장호르몬이 우선특이적으로 발현, 분비되고 있음을 알 수 있었다. 한편, 사람성장호르몬의 발현이 확인된 두 계통 (ChGH2-2, CChGH2)의 형질전환생쥐를 대상으로 하여 세대별, 산차별로 유즙내 사람성장호르몬의 함량을 조사한 결과, 3세대에 걸쳐, 또한 제1세대에서의 세차례 반복된 비유기에도 유즙내 사람성장호르몬은 지속적으로 분비되고 있었다. 이상의 결과는 형질전환생쥐에서의 외래유전자의 발현성은 반복되는 비유기와 여러 세대에 걸쳐 안정적으로 유지됨을 보여 주고 있다.

• Korean Journal of Optics and Photonics
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• v.17 no.6
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• pp.525-531
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• 2006

Synthesized 3-D CGH of a general three dimensional object is obtained by using a new 3-D coordinates transformation technique. A CCD camera is used to record several projected images of the 3-D object from different viewing angles. The recorded data are numerically calculated and processed to yield two-dimensional complex functions, which are then encoded fer the final synthesized 3-D CGH.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 1998.06a
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• pp.263-266
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• 1998

In this study, the effect of the crystallinity of polyimide (Pl) and the birefringence of the nematic liquid crystal (NLC) for pretilt angle generation on rubbed PI surface containing thiophenylene moieties has been demonstrated. The induced optical retardation produced in rubbed Pl surfaces decreases on increasing the imidization temperature. We suggest that the pretilt angle of 4-cyano-4'-n-pentybiphenyl (5CB) depends on the crystallinity of the rubbed Pl. The order of pretilt angle in NLCs is 5CB>PCH5$\geq$CCH5 at rubbing strength(RS)=150mm (medium rubbing) on rubbed PI surfaces. We suggest that on rubbed Pl surfaces the pretilt angle increases with increasing number of phenylrings in the NLC core.