• Animal Bioscience
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• v.35 no.3
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• pp.367-376
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• 2022

Objective: The highly pathogenic avian influenza virus (HPAIV) is a threat to the poultry industry as well as the economy and remains a potential source of pandemic infection in humans. Antiviral genes are considered a potential factor for HPAIV resistance. Therefore, in this study, we investigated gene expression related to cytokine-cytokine receptor interactions by comparing resistant and susceptible Ri chicken lines for avian influenza virus infection. Methods: Ri chickens of resistant (Mx/A; BF2/B21) and susceptible (Mx/G; BF2/B13) lines were selected by genotyping the Mx dynamin like GTPase (Mx) and major histocompatibility complex class I antigen BF2 genes. These chickens were then infected with influenza A virus subtype H5N1, and their lung tissues were collected for RNA sequencing. Results: In total, 972 differentially expressed genes (DEGs) were observed between resistant and susceptible Ri chickens, according to the gene ontology and Kyoto encyclopedia of genes and genomes pathways. In particular, DEGs associated with cytokine-cytokine receptor interactions were most abundant. The expression levels of cytokines (interleukin-1β [IL-1β], IL-6, IL-8, and IL-18), chemokines (C-C Motif chemokine ligand 4 [CCL4] and CCL17), interferons (IFN-γ), and IFN-stimulated genes (Mx1, CCL19, 2'-5'-oligoadenylate synthase-like, and protein kinase R) were higher in H5N1-resistant chickens than in H5N1-susceptible chickens. Conclusion: Resistant chickens show stronger immune responses and antiviral activity (cytokines, chemokines, and IFN-stimulated genes) than those of susceptible chickens against HPAIV infection.

• Journal of the Korean Chemical Society
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• v.38 no.5
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• pp.345-350
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• 1994

The $v_{a^+}$ Amide II combination band of thioacetamide has been used to evaluate thermodynamic parameters of the hydrogen bonding of thioacetamide(TA) with pyridine(Py), quinoline(Qu), and acridine(Ac) in $CHCl_3$ and $CCl_4$ over the temperature range from 5$^{\circ}C$ to 55$^{\circ}C$. This combination band was resolved into two Lorentzian-Gaussian product bands which have been identified withmonomeric TA and hydrogen bonded TA. The thermodynamic parameters for the hydrogen bonded TA were determined by computer analysis of concentration and temperature dependent spectra. The standard enthalpies for the 1 : 1 hydrogen bonded complex of TA to pyridine, quinoline, and acridine in $CHCl_3$ have been found to be -7.6 kJ/mol, -6.5 kJ/mol, and -5.4 kJ/mol, respectively. And the standard enthalpies for the 1: 1 hydrogen bonded complex of TA to pyridine and quinoline in $CCl_4$ have been found to be -13.3kJ/mol, and -12.0kJ/mol, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.27 no.6
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• pp.696-703
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• 1994

To utilize waste of squid effectively, antioxidant properities of squid viscera were elucidated. Major fatty acids of total lipid, neutral lipid and phospholipid were $C_{16:0},\;C_{18:1}\;and\;C_{20:5},\;C_{22:6}$ and consisted $63{\sim}71\%$ of total fatty acids. Total lipid did not show significant antioxidant activity when added to the fish oil at the concentration below $5.0\%$, Antioxidant activity of hexane extract was lower than total lipid or tetra carbon chloride-methanol($CCl_4-MeOH$) extract. Extracts with $CCl_4-MeOH$ exerted higher antioxidant activity as the methanol ratio was increased, suggesting that polar lipid plays and important role.

• Journal of Veterinary Clinics
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• v.24 no.1
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• pp.10-14
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• 2007

The present study was performed to elucidate the recovery effect of needle-acupuncture (needle-AP) and moxibustion treatments at Gan Shu (BL18) on $CCl_4$ induced hepatic injury in dogs, Total 9 clinically healthy experimental dogs (1 to 2 years old, 2.3-5.3kg body weight) were divided into control (3 dogs), needle-AP (3 dogs) and moxibustion (3 dogs) groups, respectively. Hepatic iniw was induced by intraperitoneal injection with $CCl_4$ (olive oil : $CCl_4=1:1,\;1ml/kg$, once/day). As far the treatments in each group, control group was not treated at all after induction of the hepatic injury till the end of experiment. Needle-Af group was treated by AP at BL18 (once/day, 20 minutes) from the next day of induction of the hepatic injury for 11 days. Moxibustion group was also treated with commercial moxa at BL18 (once/day, 20minutes) for 11days. The changes of the serum alanine transaminase(ALT), aspartate transaminase(AST) and gamma glutamyl transpeptidase (GGT) activities were investigated on pre, 0, 1, 3, 5, 7 and 11 days after hepatic injury, respectively. Histopathological changes were also investigated in the liver tissues on day 11 in experimental and control groups. The results obtained in the present study were as follows. In needle-AP group, they showed significant lower values on the 1st (p<0.05) and $3^{rd} day (p <0.05)$ in serum ALT activities, and the $11^{th}$ day in serum GGT activities, compared with those of control group, respectively. However, the significances were not detected in serum AST activities of needle-AP group by comparison with those of control group. In moxibustion group, they showed significant low values on the $1^{st}(p<0.05)$, 3rd day (p<0.05) and $5^{th}$ day (p<0.05) in serum ALT activities, compared with those of control group, respectively. However, significances were not detected in serum GGT and AST activities of moxibustion group by comparison with those of control group. Among the experimental groups, the significant low values were found on $1^{st}\;and\;3^{rd}$ day in serum ALT activities (p<0.05), and $3^{rd}\;and\;5^{th}$ day in serum GGT activities (p<0.05) in needle-AP group, compared by those of moxibustion group, respectively. In addition, the marked recovery findings of histopathological changes in experimental groups were found, compared by those of control group: mild vacuolar degeneration and necrosis findings in needle-AP group, and moderate vacuolar degeneration and necrotic findings in moxibustion group were found, in contrast to severe changes such as accumulation of bile juice, vacuolar degeneration and necrotic findings observed in control group. In conclusion, needle-AP and moxibustion treatments at BL-18 were effective for the recovery for $CCl_4$ induced hepatic injury and needle-AP was more effective than that of moxibustion in dogs.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.27 no.11
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• pp.1986-1996
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• 2003

Methodology based on the elastic-plastic fracture mechanics has been widely accepted in predicting the critical crack length(CCL) of pressure tubes of CANDU nuclear plants. A conservative estimate of CCL is obtained by employing the J-resistance curves measured with the specimens satisfying plane strain condition as suggested in the ASTM standard. Due to limited thickness of the pressure tubes the curved compact tension(CT) specimens taken out from tile pressure tube have been used in obtaining J-resistance curves. The curved CT specimen inevitably introduce slant fatigue crack during precracking. Hence, effect of specimen geometry and slant crack on J-resistance curve should be explored. In this study, the difference of J integral values between the standard CT specimens satisfying plane strain condition and the nonstandard curved CT with limited thickness (4.2mm) is estimated using finite element analysis. The fracture resistance curves of Zr-2.5Nb obtained previously by other authors are critically discussed. Various finite element analysis were conducted such as 2D analysis under plane stress and plane strain conditions and 3D analysis for flat CT, curved CT with straight crack and curved CT with slant crack front. J-integral values were determined by local contour integration near the crack tip, which was considered as accurate J-values. J value was also determined from the load versus load line displacement curve and the J estimation equation in the ASTM standard. Discrepancies between the two values were shown and suggestion was made for obtaining accurate J values from the load line displacement curves obtained by the curved CT specimens.

• The Korean Journal of Mycology
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• v.20 no.4
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• pp.347-353
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• 1992

Hepatoprotective effects of polysaccharides extracted from liquid cultured mycelia were screened by measuring the glutamic pyruvate transaminase activity of the primary cultured rat hepatocytes intoxicated with carbon tetrachloride. Sixty among 75 isolates of higher fungi showed to be hepatoprotective effect, and these were 13 of Ganoderma lucidum, 5 of Lentinus edodes, 1 of Pleurotus ostereatus, 4 of Coriolus versicolor, 2 of Lyophyllum spp., 7 of Grifora frondosa, 3 of Agaricus spp., 14 of Schizophyllum commune and 11 of Cordyceps spp.. Especially, 10 isolates, Ganoderma lucidum IY003 and IY009, Lentinus edodes IY103, Lyophyllum sp. IY402, Agaricus sp. IY701 and IY703, Schizophyllum commune IY804, IY810 and IY818, Cordyceps sp. IY902, were indicated below 80% of glutamic pyruvate transaminase activity.

• Journal of Veterinary Clinics
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• v.24 no.2
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• pp.164-168
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• 2007

This study was performed to establish the recovery effect of So Si Ho Tang on hepatic injury in dogs. Clinically healthy eight dogs (1-3 yrs old, 3-5 kg) were divided into control group (n=3) and experimental group (50 mg/kg, n=5). Hepatic injury was induced by administration of $CCl_4$ in all groups. Control group was received no treatment after hepatic injury and experimental group was orally administered with So Si Ho Tang at a dose of 50 mg/kg. The change of serum ALT and AST activities was examined before hepatic injury and on day 0, day 5 and day 12 after administration of So Si Ho Tang. Histopathologic examinations were performed on day 12. As a result, significant changes in serum ALT were found on day 5(p<0.05) and day 12(p<0.05), compared with those of control group, respectively. In addition, significant change in serum AST was found on day 1 (p<0.05) and day 5 (p<0.05), compared with those of control group, respectively. In histopathologic examination, mild hemorrhage and fatty degeneration and vacuolization were observed in experimental group in contrast to those of control group. Accordingly, it was suggested that administration of So Si Ho Tang was effective for hepatic injury induced by $CCl_4$ in dogs.

• Food Science and Preservation
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• v.31 no.4
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• pp.554-564
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• 2024

The effects of extracting solvents on the physicochemical properties of vegetable oils extracted from four oil seed plants, namely Dennettia tripetala, Dacryodes edulis, Cola rostrata, and Persea americana, were studied. Vegetable oils were extracted using the Soxhlet method. The oils were used for determining % yield, acid value (AV), iodine value (IV), saponification value (SV), electrical conductivity (EC), and pH. The results revealed that the range of the mean % yield of oils extracted using hexane, carbon tetrachloride (CCl₄), petroleum ether, acetone, and methanol, respectively, were 7.5-12.0, 9.0-22.0, 7.5-27.5 and 12.0-37.5 for the four oil Seeds respectively. Mean AVs of oils in mg KOH/g across the solvents were 3.1-3.7, 3.1-3.8, 2.5-3.9 and 2.4-2.8 for Cola rostrata, Dacryodes edulis, Dennettia tripetala and Persea americana respectively. Mean IVs of oils in gI2/100 g across the solvents were 33.25-33.97, 33.06-33.35, 32.06-33.76 and 33.00-34.00 for the four oil seeds, respectively. Mean SVs in mg KOH/g across the solvents were 191.00-197.44, 190.74-198.31, 194.11-202.52, and 182.23-199.44, respectively. Mean EC values ranged 0.31-0.32, 0.30-0.33, 0.30-0.33, and 0.31-0.32 µS/cm across the solvents, respectively. Mean pH values ranged from 6.1-6.4, 4.6-6.3, 6.2-6.4, and 6.1-6.3 across the solvents for the oils, respectively. The AVs of the oils suggest that they are edible oils, the IVs classify the oils as non-drying oils suitable for paint making, and SVs reveal that the oils are good for soap making. Hexane, petroleum ether, and CCl4 are suitable for oil extraction industrially, while D. edulis, D. tripetala, and P. Americana oils are economically viable oil resources due to their high percentage yield, SV and IV.

• Nutrition Research and Practice
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• v.4 no.2
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• pp.93-98
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• 2010

Our previous study demonstrated that methanolic extract of Chrysanthemum zawadskii Herbich var. latilobum Kitamura (Compositae) has the potential to induce detoxifying enzymes such as NAD(P)H:(quinone acceptor) oxidoreductase 1 (EC 1.6.99.2) (NQO1, QR) and glutathione S-transferase (GST). In this study we further fractionated methanolic extract of Chrysanthemum zawadskii and investigated the detoxifying enzyme-inducing potential of each fraction. The fraction (CZ-6) shown the highest QR-inducing activity was found to contain (+)-(3S,4S,5R,8S)-(E)-8-acetoxy-4-hydroxy-3-isovaleroyloxy-2-(hexa-2,4-diynyliden)-1,6-dioxaspiro [4,5] decane and increased QR enzyme activity in a dose-dependent manner. Furthermore, CZ-6 fraction caused a dose-dependent enhancement of luciferase activity in HepG2-C8 cells generated by stably transfecting antioxidant response element-luciferase gene construct, suggesting that it induces antioxidant/detoxifying enzymes through antioxidant response element (ARE)-mediated transcriptional activation of the relevant genes. Although CZ-6 fraction failed to induce hepatic QR in mice over the control, it restored QR activity suppressed by $CCl_4$ treatment to the control level. Hepatic injury induced by $CCl_4$ was also slightly protected by pretreatment with CZ-6. In conclusion, although CZ-6 fractionated from methanolic extract of Chrysanthemum zawadskii did not cause a significant QR induction in mice organs such as liver, kidney, and stomach, it showed protective effect from liver damage caused by $CCl_4$.

• KSBB Journal
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• v.19 no.1
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• pp.12-16
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• 2004

Recently the measurement of biophoton emission has attracted increasing attention in the study on physiological state of biological systems. We report the measurements of biophoton emission from the mouse fatty liver induced by carbon tetrachloride, CCl$_4$. The hepatotoxin, CCl$_4$ in olive oil, was injected intraperitoneally into two groups of ICR mice which were made of 6 mice in each group. The control groups corresponding to the treated groups were prepared with the injections of olive oil only. After the injections, livers of two groups were extracted and measured biophoton emission in 24 hours and 72 hours later, respectively. We also extracted the plasma in the blood and measured the transaminase activity. Results show that biophoton emission from the livers in 24-hour treated group is 69.3${\pm}$21.2 counts/min/$\textrm{cm}^2$, which is two times more larger than that in 24-hour control group, 29.5${\pm}$5.9 counts/min/$\textrm{cm}^2$ Biophoton emission from the livers in 72-hour treated group is 37.0${\pm}$14.8 counts/min/$\textrm{cm}^2$. These biophoton results correlate with those of the biochemical assays. We conclude that biophoton emission can be used as a biomarker of mouse fatty liver induced by CCl$_4$.