• Title/Summary/Keyword: CCD observation

Search Result 132, Processing Time 0.024 seconds

Physical Property and Morphology Observation of HepG2 Cells by Various Concentration of Paraquat (파라쿼트 농도에 따른 HepG2 세포의 물리적 특성 변화와 실시간 모폴로지 관찰)

  • Lee, Dong-Yun;Kang, Hyen-Wook;Muramatsu, Hiroshi;Kwon, Young-Soo
    • Proceedings of the KIEE Conference
    • /
    • 2009.07a
    • /
    • pp.1232_1233
    • /
    • 2009
  • Paraquat is well-known to cause hepatotoxic responses in human and other mammal species. In solution, it forms free radicals and charge-transfer complex of which formation plays an important role in determination of its biological activity in the presence of various anions. The HepG2 cells were cultured onto a quartz crystal sensor which is possible to detect the density and a viscosity changes using the resonance frequency (F) and the resonance resistance (R). The plot of F-R diagram is able to explain the rheological change of cells onto the surface of the quartz crystal sensor. In this paper, we investigated the physical properties of the HepG2 cells cultured onto a ITO electrode of the quartz crystal sensor according to the paraquat injection at various concentrations (100 mM, 10 mM, 1 mM). We also observed the morphological changes with a micro CCD camera, simultaneously. The HepG2 cells were cultured onto the ITO electrode surface of the quartz crystal modified a collagen film in $CO_2$ incubator. After the paraquat injection, we observed the changes of the morphologies by the micro CCD camera depending on time and analyzed the physical changes of cells on the electrode surface of quartz crystal using F-R diagram. From all results, we proved the effect of paraquat at various concentrations which is led to an apoptosis such as weakening and death of the cells by oxidation and reduction reaction that were produced the superoxide anions and other free radicals.

  • PDF

Auto-Guiding System for McDonald Otto Struve Telescope

  • Kim, Eun-Bin;Park, Won-Kee;Kim, Jin-Young;Oh, Hee-Young;Choi, Chang-Su;Pak, Soo-Jong;Im, Myung-Shin;Kuehne, John
    • The Bulletin of The Korean Astronomical Society
    • /
    • v.35 no.1
    • /
    • pp.38.1-38.1
    • /
    • 2010
  • McDonald 2.1m Otto Struve Telescope is located in the Davis Mountains, 450 miles west of Austin, Texas. The telescope was built in 1938, but it is still in demand today. CQUEAN (Camera for QUasar in Early uNiverse) will be attached on this telescope and perform Y-band imaging observations. Dynamics study of the telescope shows that tracking errors are 0.1 arcsec/100sec in declination direction and 0.4 arcsec/100sec in R.A. direction. In order to allow a long exposure (> a few minutes) of a target field, we are making auto-guiding system for the 2.1m telescope. The auto-guiding system of CQUEAN will be connected with TCS of the telescope. The expected number of stars on the CCD field (2.97 square arcminutes) is about 1.2 stars which are brighter than magnitude 17.5 in 2.97 square arcminutes. For more effective observation, we plan to implement moving mechanism in guiding system so that guide CCD camera can see wider off-axis fields.

  • PDF

Focal Reducer for McDonald Otto Struve Telescope

  • Lim, Ju-Hee;Kim, Young-Ju;Park, Won-Kee;Kim, Jin-Young;Chang, Seung-Hyuk;Pak, Soo-Jong;Im, Myung-Shin
    • The Bulletin of The Korean Astronomical Society
    • /
    • v.35 no.1
    • /
    • pp.36.2-36.2
    • /
    • 2010
  • The CQUEAN (Camera for QUasars in EArly uNiverse) is an optical CCD camera optimized for observation of high redshift objects. It is going to be attached to the cassegrain focus of 2.1m telescope at McDonald Observatory, USA. We are making a focal reducer for CQUEAN to secure a larger field of view. The focal reducer is composed of four spherical lens, and it reduces the focal length of telescope by one third. We designed the lens configuration, performed tolerance analysis, and estimated the optical performance with ZEMAX. The differences in optical performace with/without filters were also investigated. The result from ZEMAX shows that the system has ample margin of errors for median seeing of 1.2" at McDonald observatory. Even with aberration and alignment tolerance, the performance is better than the original requirement. The lenses are now being made, and the lens barrel and an adapter for assembly of the Andor CCD camera and the filter wheel is now under designing process. We expect that the manufacturing of the focal reducer system as well as its optical test will be finished by April 2010.

  • PDF

Fire-Smoke Detection Based on Video using Dynamic Bayesian Networks (동적 베이지안 네트워크를 이용한 동영상 기반의 화재연기감지)

  • Lee, In-Gyu;Ko, Byung-Chul;Nam, Jae-Yeol
    • The Journal of Korean Institute of Communications and Information Sciences
    • /
    • v.34 no.4C
    • /
    • pp.388-396
    • /
    • 2009
  • This paper proposes a new fire-smoke detection method by using extracted features from camera images and pattern recognition technique. First, moving regions are detected by analyzing the frame difference between two consecutive images and generate candidate smoke regions by applying smoke color model. A smoke region generally has a few characteristics such as similar color, simple texture and upward motion. From these characteristics, we extract brightness, wavelet high frequency and motion vector as features. Also probability density functions of three features are generated using training data. Probabilistic models of smoke region are then applied to observation nodes of our proposed Dynamic Bayesian Networks (DBN) for considering time continuity. The proposed algorithm was successfully applied to various fire-smoke tasks not only forest smokes but also real-world smokes and showed better detection performance than previous method.

Development of a Data Reduction Algorithm for Optical Wide Field Patrol (OWL) II: Improving Measurement of Lengths of Detected Streaks

  • Park, Sun-Youp;Choi, Jin;Roh, Dong-Goo;Park, Maru;Jo, Jung Hyun;Yim, Hong-Suh;Park, Young-Sik;Bae, Young-Ho;Park, Jang-Hyun;Moon, Hong-Kyu;Choi, Young-Jun;Cho, Sungki;Choi, Eun-Jung
    • Journal of Astronomy and Space Sciences
    • /
    • v.33 no.3
    • /
    • pp.221-227
    • /
    • 2016
  • As described in the previous paper (Park et al. 2013), the detector subsystem of optical wide-field patrol (OWL) provides many observational data points of a single artificial satellite or space debris in the form of small streaks, using a chopper system and a time tagger. The position and the corresponding time data are matched assuming that the length of a streak on the CCD frame is proportional to the time duration of the exposure during which the chopper blades do not obscure the CCD window. In the previous study, however, the length was measured using the diagonal of the rectangle of the image area containing the streak; the results were quite ambiguous and inaccurate, allowing possible matching error of positions and time data. Furthermore, because only one (position, time) data point is created from one streak, the efficiency of the observation decreases. To define the length of a streak correctly, it is important to locate the endpoints of a streak. In this paper, a method using a differential convolution mask pattern is tested. This method can be used to obtain the positions where the pixel values are changed sharply. These endpoints can be regarded as directly detected positional data, and the number of data points is doubled by this result.

PHOTOMETRIC OBSERVATIONS AND LIGHT CURVE ANALYSIS OF BL ERIDANI (BL ERIDANI의 측광관측과 광도곡선 분석)

  • Han, Won-Yong;Yim, Hong-Suh;Lee, Chung-Uk;Youn, Jae-Hyuck;Yoon, Joh-Na;Kim, Ho-Il;Moon, Hong-Kyu;Byun, Yong-Ik;Park, Sun-Youp
    • Journal of Astronomy and Space Sciences
    • /
    • v.23 no.4
    • /
    • pp.319-326
    • /
    • 2006
  • We present light curves of a short period binary system BL Eridani. The light curves were observed with VRI filters by a 50cm wide field robotic telescope at Siding Spring Observatory (SSO), equipped with a 2K CCD camera, which was developed by Korea Astronomy and Space Science Institute (KASI), and Yonsei University Observatory(YUO). The photometric observations were made on 6 nights by automatic operation mode and remote observation mode at SSO and KASI in Korea, respectively. We obtained new VRI CCD light curves and new 5 times of minima, and analyzed the light corves with the Wilson & Deviney (1971) binary 2005 version and derived the new photometric solutions. The mass ratio q=0.48 in this study shows different value with earlier investigators. According to the model analysis, it is considered that the BL Eri system is currently undergoing contact stage of the two binary components, rather than near-contact stage.

ALL-SKY OBSERVATION OF THE 2001 LEONID METEOR STORM: 1. METEOR MAGNITUDE DISTRIBUTION (전천 카메라를 이용한 2001 사자자리 유성우 관측: 1. 유성 등급 분포)

  • 김정한;정종균;김용하;원영인;천무영;임홍서
    • Journal of Astronomy and Space Sciences
    • /
    • v.20 no.4
    • /
    • pp.283-298
    • /
    • 2003
  • The 2001 Leonid meteor storm has been observed all over the world, and its most intense flux since the last few decades has caused great interest among both laymen and experts. Especially, its maximum hours occurred at dawn hours of Nov. 19 in the east Asia, during which moonless clear night at the Mt. Bohyun allowed us near perfect condition of observation. Observation was carried out in the period of 01:00∼05:40(KST), which include the predicted maximum hours, with all-sky camera installed for upper atmospheric airglow research. Tn this paper we analyze 68 all-sky images obtained in this period, which contain records of 172 meteors. Utilizing the zenith hourly rate(ZHR) of 3000 and magnitude distribution index of 2, which were reported to International Meteor Organization by visible observers in the east Asia, we estimate the limiting magnitude of about 3 for meteors detected in our all-sky images. We then derive magnitudes of 83 meteors with clear pixel brightness outlines among the initially detected 172 meteors by comparing with neighbor standard stars. Angular velocities of meteors needed for computing their passing times over an all-sky image are expressed with a simple formula of an angle between a meteor head and the Leonid radiant point. The derived magnitudes of 83 meteors are in the range of -6∼-1 magnitude, and its distribution shows a maximum new -3mag. The derived magnitudes are much smaller than the limiting magnitude inferred from the comparison with the result of naked-eye observations. The difference may be due to the characteristic difference between nearly instantaneuous naked-eye observations and CCD observations with a long exposure. We redetermine magnitudes of the meteors by adjusting a meteor lasting time to be consistent with the naked-eye observations. The relative distribution of the redetermined magnitudes, which has a maximum at 0 mag., resembles that of the magnitudes determined with the in-principle method. The relative distribution is quite different from ones that decrease monotonically with decreasing magnitudes for meteors(1∼6) sensitive to naked-eye observations. We conclude from the magnitude distribution of our all-sky observation that meteors brighter than about 0 mag., appeared more frequently during the 2001 Leonid maximum hours. The frequent appearance of bright meteors has significantly important implication for meteor research. We noted, however, considerably large uncertainties in magnitudes determined only by comparing standard stars due to the unknown lasting time of meteors and the non-linear sensitivity of all-sky camera.

FOCAL REDUCER FOR CQUEAN (Camera for QUasars in EArly uNiverse)

  • Lim, Juhee;Chang, Seunghyuk;Pak, Soojong;Kim, Youngju;Park, Won-Kee;Im, Myungshin
    • Journal of The Korean Astronomical Society
    • /
    • v.46 no.4
    • /
    • pp.161-172
    • /
    • 2013
  • A focal reducer is developed for CQUEAN (Camera for QUasars in EArly uNiverse), which is a CCD imaging system on the 2.1 m Otto Struve telescope at the McDonald observatory. It allows CQUEAN to secure a wider field of view by reducing the effective focal length by a factor of three. The optical point spread function without seeing effects is designed to be within one pixel ($0.283^{\prime\prime}$) over the field of view of $4.82^{\prime}{\times}4.82^{\prime}$ in optimum wavelength ranges of 0.8-1.1 ${\mu}m$. In this paper, we describe and discuss the characteristics of optical design, the lens and barrel fabrications and the alignment processes. The observation results show that the image quality of the focal reducer confirms the expectations from the design.

Optical Observation of Cavitation Phenomena in Diesel Fuel Injection Pumps (디젤 엔진 연료 분사 펌프 캐비테이션 현상의 가시화 연구)

  • Ryu, Seung-Hyup;Kim, Dong-Hun;Kim, Byung-Seok;Park, Tae-Hyung
    • Journal of Advanced Marine Engineering and Technology
    • /
    • v.35 no.4
    • /
    • pp.460-467
    • /
    • 2011
  • Cavitation phenomena during the spill process of the Bosch type fuel injection pump for medium-speed diesel engine were investigated by optical observations. Typically, these phenomena can cause a surface damage with material removal or round-off at the plunger and barrel port etc., and may shorten their expected life time. The images, which were recorded with high speed CCD camera and borescope, show that the plunger damage is mainly affected by fountain-like cavitation generated before the end of delivery. And the damages of barrel port and deflector are caused by jet-type cavitation generated after end of delivery.

A Capturing Algorithm of Moving Object using Single Curvature Trajectory (단일곡률궤적을 이용한 이동물체의 포획 알고리즘)

  • Choi Byoung-Suk;Lee Jang-Myung
    • Journal of Institute of Control, Robotics and Systems
    • /
    • v.12 no.2
    • /
    • pp.145-153
    • /
    • 2006
  • An optimal capturing trajectory for a moving object is proposed in this paper based on the observation that a single-curvature path is more accurate than double-or triple-curvature paths. Moving distance, moving time, and trajectory error are major factors considered in deciding an optimal path for capturing the moving object. That is, the moving time and distance are minimized while the trajectory error is maintained as small as possible. The three major factors are compared for the single and the double curvature trajectories to show superiority of the single curvature trajectory. Based upon the single curvature trajectory, a kinematics model of a mobile robot is proposed to follow and capture the moving object, in this paper. A capturing scenario can be summarized as follows: 1. Motion of the moving object has been captured by a CCD camera., 2. Position of the moving object has been estimated using the image frames, and 3. The mobile robot tries to follow the moving object along the single curvature trajectory which matches positions and orientations of the moving object and the mobile robot at the final moment. Effectiveness of the single curvature trajectory modeling and capturing algorithm has been proved, through simulations and real experiments using a 2-DOF wheel-based mobile robot.