• Journal of Microbiology and Biotechnology
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• 제8권1호
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• pp.96-100
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• 1998

Pseudomonas sp. S-47 degraded 4-chlorobenzoate (4CBA) to 4-chlorocatechol (4CC) that was subsequently ring-cleaved to form 5-chloro-2-hydroxymuconic semialdehyde. These intermediate compounds were identified by GC-mass spectrometry and UV-visible spectrophotometry. 5-chloro-2-hydroxymuconic acid converted from 5-chloro-2- hydroxymuconic semialdehyde (5C-2HMS) was dechlorinated to produce 2-hydroxypenta-2,4-dienoic acid (2HP-2,4DA) by the strain. These results indicate that Pseudomonas sp. S-47 degrades 4CBA to 2HP-2,4DA via a novel pathway including the meta-cleavage of 4CC and dechlorination of 5C-2HMS.

• 한국정보과학회:학술대회논문집
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• 한국정보과학회 2006년도 한국컴퓨터종합학술대회 논문집 Vol.33 No.1 (A)
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• pp.22-24
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• 2006

마이크로어레이는 전체 유전체 수준의 mRNA 발현 여부에 대한 측정이 가능하다는 점에서 분자생물학의 실험 도구로서 가장 강력한 도구 중에 하나로 부각되어 있다. 현재까지 마이크로어래이의 결과로부터 유사한 발현 패턴을 찾기 위한 여러 가지 바이클러스터링 알고리즘들이 개발되어 왔다. 하지만 대다수의 알고리즘들이 최적의 바이클러스터들을 찾기보다는 일정 수준의 가능한 바이클러스터의 결과만을 제시하고 있다. 본 논문에서는 다른 개체집단들과 상호 진화하는 공진화적 학습에 의한 진화연산 기법을 통하여 유전자-조건의 매트릭스로부터 열과 행을 동시에 클러스터링하는 공진화적 바이클러스터링 알고리즘(co-evolutionary biclustering algorithm: CBA)을 제안하고자 한다. CBA는 유전자발현 데이터에서 유전자-조건의 상호의존적인 부성분들로 구성된 최적화 문제에 적합한 계산방식이라고 할 수 있다. 인간 유전자 발현 데이터에 대한 실험 결과. 제시한 알고리즘은 이전의 알고리즘에 비해 발견한 바이클러스터의 패턴 유사도에 있어서 우수한 성능을 보이고 있다.

• 대한산업공학회지
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• 제21권2호
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• pp.207-216
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• 1995

Constructing a distributed management system has its own advantages in addressing the issue of implementing a quick responsive management system in dynamically changing environment of enterprise. We suggest a basic scheduling methodology applicable to a distributed production management system. A new concept of "flexible schedule" is introduced as a tool to accommodate dynamically changing situations of job shops. Then a search technique (referred to as CSP-CBA search) is presented to obtain such a schedule for the job shop scheduling problem, which is converted into a constraint satisfaction problem(CSP), by using the constraint based analysis(CBA). This approach is tested on more than 100 test problems. The results show that the suggested approach required shorter CPU time and solved more problems in given time than another fixed schedule method.

• International Journal of Computer Science & Network Security
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• 제21권11호
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• pp.278-286
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• 2021

The aim of this paper is to examine the importance of skills development in the process of employee performance. As part of this research, we will seek to determine the nature and extent of skills development impact in improving employee performance. This research project is one of the new themes that some researchers have started in recent years. The novelty of this theme is the inclusion of the skill development factor. This factor is likely to have a positive impact on employee motivation and performance. Some of the factors already known to have an impact on employee performance, such as motivation, career development, training, and experience, will be adopted. It is assumed that the results of this research will have a positive impact on employee performance and employee retention.

• International Journal of Computer Science & Network Security
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• 제22권3호
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• pp.147-154
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• 2022

Actually, brand personality is more and more important in marketing field. Considering the employee as consumer this paper will study the effect of employee brand personality on the intention to send a job application. In this research we tried to make the difference by following the qualitative methodology. we did ten interviews with IT engineering student in terminal classes and after thematique analysis tow thems emerged in relation with the brand personality factors which are sincerity and excitement. people are attracted by firms inspiring confidence and sincerity an exciting environment this is an important finding for firms who want to attract talented people. however, it important for enterprises in the IT filed to focus all their communication actions on the sincerity and excitement

• 한국가스학회지
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• 제15권1호
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• pp.15-21
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• 2011

본 논문에서는 정량적 위험성 평가 결과 위험경감조치로 제시한 ILI (In-line inspection, 배관내부검사)가 합리적으로 이행가능한지를 판정하기 위해 비용-편익 분석을 실시하였으며, 비용-편익 분석을 통해 ILI의 합리적인 이행시기를 결정하였다. 본 논문에서 편익의 산정은 인간의 생명 가치를 측정하는 VPF (Value of Preventing a Fatality; 사망자를 막는 가치)를 이용하였으며, 국내의 도시가스 고압배관의 위험경감조치에 대한 비용-편익 분석에 적정한 VPF 값은 20억원으로 하였다. 두 개의 사례연구 결과 위험경감조치로 제시된 ILI의 이행시기는 13년을 주기로 2회 (13년 및 25년) 또는 15년이 되는 해 1회 실시하는 것이 가장 합리적임을 알 수 있었다.

• Journal of Microbiology
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• 제35권1호
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• pp.53-60
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• 1997

Pseudomonas sp. P20 and Pseudomonas sp. DJ-12 isolated from the polluted environment are capable of degrading biphenyl and 4-chlorobiphenyl (4CB) to produce benzoic acid and 4-chlorobenzoic acid (4CBA) respectively, by pcbABCD-encoded enzymes. 4CBA can be further degraded by Pseudomonas sp. DJ-12, but not by Pseudomonas sp P20. However, the meta-cleavage activities of 2, 3-dihydroxybiphenyl (2, 3-DHBP) and 4-chloro-2, 3-DHBP dioxygenases (2, 3-DHBD) encoded by pcbC in Pseudomonas sp. P20 were stronger than Pseudomonas sp. DJ-12. In this study, the pcbC gene encoding 2, 3-DHBD was cloned from the genomic DNA of Pseudomonas sp. P20 by using pKT230. A hybrid plasmid pKK1 was constructed and E. coli KK1 transformant was selected by transforming the pKK1 hybrid plasmid carrying pcbC into E. coli XL1-Blue. By transferring the pKK1 plasmide of E. coli KK1 into Pseudomonas sp. DJ-12 by conjugation, a recombinant strain Pseudomonas sp. P20, Pseudomonas sp. DJ-12, and the recombinant cell assay methods. Pseudomonas sp. DJ12-C readily degraded 4CB and 2, 3-DHBP to produce 2-hydroxy-6-oxo-6-phenylhexa-2, 4-dienoic acid (HOPDA), and the resulting 4CBA and benzoic acid were continuously catabolized. Pseudomonas sp. DJ12-C degraded 1 mM 4CB completely after incubation for 20 h, but Pseudomonas sp. P20 and Pseudomonas sp. DJ-12 showed only 90% and Pseudomonas sp. DJ-12 had, but its degradation activity to 2, 3-DHBP, 3-methylcatechol, and catechol was improved.

• 한국가스학회지
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• 제15권2호
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• pp.40-46
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• 2011

위험성 평가에서 비용-편익분석의 목적은 위험성 평가를 통해 도출된 부가적인 위험감소조치의 편익이 그 조치를 수행하는 데 든 비용보다 많음을 확인하고, 그 조치를 이행하는 것이 적절함을 보여주는 데 있다. 본 논문에서는 도시가스 고압배관의 위험성 평가에 따라 도출한 위험감소조치를 이행함에 있어 가장 효과적이고 합리적인 조치를 선정하기 위하여 비용-편익 분석을 실시하였다. 도출한 위험감소조치를 적용하여 위험성 평가를 다시 실시한 결과, 배관부식방지조치, MOV 설치 및 타공사 방지조치를 하도록 한 10번 조치가 위험감소효과가 75 %로 가장 컸으며, 비용-편익 분석 결과 합리적으로 실행가능한 것으로 판단되어 본 논문의 연구대상 배관의 위험감소조치로 선정하였다.

• 정보화연구
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• 제10권4호
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• pp.517-536
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• 2013

전쟁 패러다임이 플랫폼 중심 전에서 네트워크 중심 전으로 변화함에 따라 네트워크 중심 전을 보다 효과적으로 수행하기 위한 소요제기 방식 또한 기존 플랫폼, 무기체계 중심의 상향식 방식에서 능력 개념을 적용한 하향식 방식으로 변화하고 있다. 하향식 소요제기 방식은 능력기반기획 및 능력기반평가 등을 통해 수행되는 개념이다. 그러나 이러한 개념을 방법론에 적절하게 적용하는 것은 도전이다. 따라서 기 개발된 방법론의 시사점으로부터 능력기반평가를 기반으로 능력기반기획 시 적의 능력을 기준으로 아군이 확보해야 할 적절한 능력을 도출할 수 있는 새로운 방법론을 제안한다. 본 논문에서 제안하는 방법론은 군사작전 분야 뿐 아니라 치안확보, 자연재해 분야의 능력기반평가기반 능력기반기획 시 적용 할 수 있다.

• Journal of Microbiology and Biotechnology
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• 제31권2호
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• pp.327-337
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• 2021

Fibrinolytic enzymes with a direct mechanism of action and safer properties are currently requested for thrombolytic therapy. This paper reports on a new enzyme capable of degrading blood clots directly without impairing blood coagulation. This enzyme is also non-cytotoxic and constitutes an alternative to other thrombolytic enzymes known to cause undesired side effects. Twenty-four Bacillus isolates were screened for production of fibrinolytic enzymes using a fibrin agar plate. Based on produced activity, isolate S127e was selected and identified as B. subtilis using the 16S rDNA gene sequence. This strain is of biotechnological interest for producing high fibrinolytic yield and consequently has potential in the industrial field. The purified fibrinolytic enzyme has a molecular mass of 27.3 kDa, a predicted pI of 6.6, and a maximal affinity for Ala-Ala-Pro-Phe. This enzyme was almost completely inhibited by chymostatin with optimal activity at 48℃ and pH 7. Specific subtilisin features were found in the gene sequence, indicating that this enzyme belongs to the BPN group of the S8 subtilisin family and was assigned as AprE127. This subtilisin increased thromboplastin time by 3.7% (37.6 to 39 s) and prothrombin time by 3.2% (12.6 to 13 s), both within normal ranges. In a whole blood euglobulin assay, this enzyme did not impair coagulation but reduced lysis time significantly. Moreover, in an in vitro assay, AprE127 completely dissolved a thrombus of about 1 cc within 50 min and, in vivo, reduced a thrombus prompted in a rat tail by 11.4% in 24 h compared to non-treated animals.