• Korean Journal of Pharmacognosy
• /
• v.25 no.2
• /
• pp.121-131
• /
• 1994

Two kinds of new lectins, CATL-I and CATL-II, were partially purified from the intestine of Chlorostoma argyrostoma turbunatum by physical saline extraction, salt fractionation, ion exchange chromatography and gel filtration. CATL-I and CATL-II were purified 39.4 and 15.8 fold with a yield of 8.8 and 7.4%, respectively. On polyacrylamide gel electrophoresis, CATL-I demonstrated one major and one minor bands. This lectin agglutinated human and other animal erythrocytes nonspecifically and also agglutinated murine splenic lymphocytes. Carbohydrate specificity of the lectins was determined by inhibition of the agglutinability by methyl-${\alpha}-_D$-galactopyranoside and $_L-rhamnose$ at a final concentration of 6 mM. The lectins contained relatively high amounts of acidic amino acids, but the contents of sulfur containing amino acids were very low or was not estimated. Immunochemical studies were carried out to identify some properties of marine animal lectins.

• Journal of the Korean Association of Geographic Information Studies
• /
• v.15 no.2
• /
• pp.46-56
• /
• 2012

Recently, when software architecture is designed, the efficiency of reusability is emphasized. The reusability of the design can raise the quality of GIS software, and reduce the cost of maintenance. Because the object oriented GoF design pattern provides the class hierarchy that can represent repetitively, the importance is emphasized more. This method that designs the GIS software can be applied from various application systems. A multiple distributed sensor network system is composed of the complex structure that each node of the sensor network nodes has different functions and sensor nodes and server are designed by the combination of many classes. Furthermore, this sensor network system may be changed into more complex systems according to a particular purpose of software designer. This paper will design the CATL model by applying Facade pattern that can enhance the efficiency of reuse according to attributes and behaviors in classes in order to implement the complicated structure of the multiple distributed sensor network system based on TinyOS. Therefore, our object oriented GIS design pattern model will be expected to utilize efficiently for design, update, or maintenance, etc. of new systems by packing up attributes and behaviors of classes at complex sensor network systems.

• TTA Journal
• /
• s.92
• /
• pp.32-35
• /
• 2004

• Journal of Microbiology
• /
• v.39 no.3
• /
• pp.168-176
• /
• 2001

Five different types of catalases from photosynthetic bacterium Rhodospirillum rubrum S1 grown aerobically in the dark were found in this study, and designated Catl (350 kDa), Cat2 (323 kDa), Cat3 (266 kDa), Cat4 (246 kDa), and Cat5 (238 kDa). Analysis of native PAGE revealed that Cat2, Cat3, and Cat4 were also produced in the cells anaerobically grown in the light. It is notable that only Cat2 was expressed much more strongly in response to the anaerobic condition. Enzyme activity staining demonstrated that Cat3 and Cat4 had bifunctional catalase-peroxidase activities, while Catl, Cat2, and Cat5 were typical monofunctional catalases. S1 cells grown aerobically in the presence of malate as the sole source of carbon exhibited an apparent catalase Km value of 10 mM and a Vmax of about 705 U/mg protein at late stationary growth phase. The catalase activity of Sl cells grown in the anaerobic environment exhibited a much lower Vmax of about 109 U/mg protein at late logarithmic growth phase. The catalytic activity was stable in the broad range of temperatures (30$\^{C}$-60$\^{C}$), and pH (6.0-10.0). R. rubrum S1 was much more resistant to H$_2$O$_2$in the stationary growth phase than in the exponential growth phase regardless of growth conditions. Cells of stationary growth phase treated with 15 mM H$_2$O$_2$for 1 h showed 3-fold higher catalase activities than the untreated cells. In addition, L-glutamate induced an 80-fold increase in total catalase activity of R. rubrum S1 compared with magic acid. Through fraction analyses of S1 cells, Cat2, Cat3, Cat4 and Cat5 were found in both cytoplasm and periplasm, while Catl was localized only in the cytoplasm.

• International Journal of Safety
• /
• v.5 no.1
• /
• pp.5-9
• /
• 2006

The present study reports on the results of a risk survey of machinery safety at a shoe factory in Qingdao, China. The aim is to decrease industrial accidents and increase worker job satisfaction by searching for a change from a trend analysis and making improvements in problem areas. The first risk survey for machinery safety was carried out in April 2005. Based on the analysis of the survey results, machinery safety devices was installed in the factory by using CATlA V5. Also, we investigated job satisfaction concerning working apparatus and work tool improvement in a questionnaire about the working environment. The second survey of machinery safety was carried out in September 2005. We are in the process of comparing the first survey results with the second survey results in order to analyze decreasing trend in industrial accidents and improvement in job satisfaction. So far the data have shown improved short-term turnover and absenteeism. It means CATIVA V5 and CFR has had positive effect regarding safety in shoe manufacture industry. The survey with CATIVA V5 and CFR will be expanded to other East-Asian countries including Vietnam. The hope is that the present approach could make a significant contribution toward improved safety.

• Earthquakes and Structures
• /
• v.14 no.5
• /
• pp.411-424
• /
• 2018

This study explores the inelastic behavior of systems with flexible base. The use of a single degree of freedom system (ESDOF) with equivalent ductility to represent the response of flexible base systems is discussed. Two different equations to compute equivalent ductility are proposed, one which includes the contribution of rigid body components, and other based on the overstrength of the structure. In order to asses the accuracy of ESDOF approach with the proposed equations, the behavior of a 10-story regular building with reinforced concrete (RC) moment resisting frames is studied. Local and global ductility capacity and demands are used to study the modifications introduced by base flexibility. Three soil types are considered with shear wave velocities of 70, 100 and 250 m/s. Soil-foundation stiffness is included with a set of springs on the base (impedance functions). Capacity curves of the building are computed with pushover analysis. In addition, non linear time history analysis are used to asses the ductility demands. Results show that ductility capacity of the soil-structure system including rigid body components is reduced. Base flexibility does not modify neither yield and maximum base shear. Equivalent ductility estimated with the proposed equations is fits better the results of the numerical model than the one considering elastoplastic behavior. Modification of beams ductility demand due to base flexibility are not constant within the structure. Some elements experience reduced ductility demands while other elements experience increments when flexible base is considered. Soil structure interaction produces changes in the relation between yield strength reduction factor and structure ductility demand. These changes are dependent on the spectral shape and the period of the system with fixed and flexible base.

• Journal of Microbiology
• /
• v.44 no.4
• /
• pp.447-452
• /
• 2006

The murine ecotropic retroviral receptor has been demonstrated to function as a mouse cationic amino acid transporter 1(mCAT1), and is comprised of multiple membranespanning domains. Feral mouse (Mus dunni) cells are not susceptible to infection by the ecotropic Moloney murine leukemia virus (MoMLV), although they can be infected by other ecotropic murine leukemia viruses, including Friend MLV and Rauscher MLV. The relative inability of MoMLV to replicate in M. dunni cells has been attributed to two amino acids $(V_{214}\;and\;G_{236})$ located within the third extracellular loop of the M. dunni CAT1 receptor (dCAT1). Via the exchange of the third extracellular loop of the mCAT1 cDNA encoding receptor from the permissive mouse and the corresponding portion of cDNA encoding for the nonpermissive M. dunni receptor, we have identified the most critical amino acid residue, which is a glycine located at position 236 within the third extracellular loop of dCAT1. We also attempted to determine the role of the third extracellular loop of the M. dunni CAT1 receptor with regard to the formation of the syncytium. The relationship between dCAT1 and virus-induced syncytia was suggested initially by our previous identification of two MLV isolates (S82F in Moloney and S84A in Friend MLV), both of which are uniquely cytopathic in M. dunni cells. In an attempt to determine the relationship existing between dCAT1 and the virally-induced syncytia, we infected 293-dCAT1 or chimeric dCAT1 cells with the S82F pseudotype virus. The S82F pseudotype virus did not induce the formation of syncytia, but did show increased susceptibility to 293 cells expressing dCATl. The results of our study indicate that S82F-induced syncytium formation may be the result of cell-cell fusion, but not virus-cell fusion.

• Journal of Information Technology Applications and Management
• /
• v.30 no.1
• /
• pp.1-9
• /
• 2023

Interest in the future of the battery market is growing as Tesla announces plans to increase production of electric vehicles and to produce batteries. Tesla announced an action plan to reduce battery prices by 56% through 'Battery Day', which included expansion of factories to internalize batteries and improvement of materials and production technology. In the trend of automobile electrification, the expansion of the battery market, which accounts for 40% of the cost of electric vehicles, is inevitable, and the size of the electric vehicle battery market in 2026 is expected to increase more than five times compared to 2016. With the development of materials and process technology, the energy density of electric vehicle batteries is increasing while the price is decreasing. Soon, electric vehicles and internal combustion locomotives are expected to compete on the same line. Recently, the mileage of electric vehicles is approaching that of an internal combustion locomotive due to the installation of high-capacity batteries. In the EV battery market, Korean, Chinese and Japanese companies are fiercely competing. Based on market share in the first half of 2020, LG Chem, CATL, and Panasonic are leading the EV battery supply, and the top 10 companies included 3 Korean companies, 5 Chinese companies, and 2 Japanese companies. All-solid, lithium-sulfur, sodium-ion, and lithium air batteries are being discussed as the next-generation batteries after lithium-ion, among which all-solid-state batteries are the most active. All-solid-state batteries can dramatically improve stability and charging speed by using a solid electrolyte, and are excellent in terms of technology readiness level (TRL) among various technology alternatives. In order to increase the competitiveness of the battery industry in the future, efforts to increase the productivity and economy of electric vehicle batteries are also required along with the development of next-generation battery technology.

• Korean Journal of Microbiology
• /
• v.30 no.4
• /
• pp.291-298
• /
• 1992

Srrepromycec. corlirolar produces at least 4 catalase activity bands with different electrophoretic mobilities on polyacrylamide gel which vary during development. Spores and mycelia at stationary phase produced all the activity bands(Cat1. 760 kr); Cat3-I, 170 kD: Cat3-2, 140 kD: Cat3-3. 130 kD; Cat4, 70 kD) except for Cat2 (300 kD). Mycelia at mid-logarithmic phase produced only Cat2 and Cat3-2 bands, and mycelia at late-logarithmic phase produced bands except Catl and Cat\ulcorner. Catalase-deficient mutants were screened in S. coelicalur by H201 bubbling test following NTG mutagenesis. Wc tested sevcral non-bubbling or slow-bubbling mutants for their catalase activities. The overall activities in cell extracts decreased more than 5 fold. Activity bands in native gel selectively decreased in intensity or disappeared. In all the non-bubbling mutants testcd, Cat3-2 band decreased significantly or disappeared. suggesting that Cat3-2 is the major catalase. The selective disappearance of bands in mutants suggest that each band is governed by different genes. We purified catalase activity from -:ell extracts obtained at late-logarithmic phase. Following chromatographies on Sepharose CL-4B. DEAE Sepharose CL-6B. Phcnyl Sepharose CL-4B. and hydroxylapatite columns. only the Cat3-2 activity was obtained. The native form of Cat3-2 has molecular weight of approximately 140 kD, judged by gel electrophoresis. Thc electrophoretic mobility on SDS-polyactylamide gel suggests that this enzyme contains 2 identical subunits of 67 kD.