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Effect of Chromium Stress on Antioxidative Enzymes and Malondialdehyde Content Activities in Leaves and Roots of Mangrove Seedlings Kandelia Candel (L.) Druce

  • Rahman, Mohammed Mahabubur;Rahman, Motiur M.;Islam, Kazi Shakila;Chongling, Yan
    • Journal of Forest and Environmental Science
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    • v.26 no.3
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    • pp.171-179
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    • 2010
  • Effect of chromium (Cr) stress on antioxidant enzyme activities and malondialdehyde (MDA) content were investigated in leaves and roots of mangrove (italic (L.) Druce) seedlings. Cr toxicity effects were also assessed on young seedlings. The seedlings were grown in green house condition for three months in nutrient solution with 0, 0.5, 1, 1.5, 2, 2.5, and 3 mg $L^{-1}$ $CrCl_3$. This study showed that Cr led to the change of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) and activities at different concentrations. The activity of antioxidant enzymes in leaves of K. candel seedlings indicates that enzymes engaged in antioxidant defense in certain level especially in low concentration of Cr treatments. The activities of SOD and POD were activated by Cr in the root level, while CAT activity was inhibited. CAT activity decreased in response to high concentrations of Cr. In the present study indicated that SOD in root was active in scavenging the superoxide produced by Cr. Both in roots and leaves, an increase in malondialdehyde (MDA) content was observed with increase in metal concentration and exposure periods. Our finding indicated that the high concentration of excessive Cr supply may interfere with several metabolic processes of seedlings, causing toxicity to plants as exhibited by chlorosis, necrosis, photosynthetic impairing and finally, plant death.

Hypospadias and Megacolon in a Persian Cat (페르시안 고양이에서 발생한 요도밑 열림증과 거대결증증)

  • Kim, Sung-Eon;Choi, Ran;Park, Jasil;Yang, Hye-Mi;Hyun, Changbaig
    • Journal of Veterinary Clinics
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    • v.31 no.5
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    • pp.454-456
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    • 2014
  • A 9-month-old intact male Persian cat (3.2 kg of body weight) was referred with primary complaint of constipation. Diagnostic studies found severe constipation and megacolon. After immediate medical treatment (e.g. enema), the physical examination was performed and revealed that the unusual urethral opening was found approximately 0.5 cm ventral to the tip of the penis where a 3.5 fr tomcat catheter was easily advanced into the bladder. The case was diagnosed as hypospadias. The cat was treated with castration and medical therapy for constipation. To our best knowledge, this case is the first case report for feline hypospadias complicated with chronic constipation in Korea.

Comparison of CT numbers between cone-beam CT and multi-detector CT (Cone-beam CT와 multi-detector CT영상에서 측정된 CT number에 대한 비교연구)

  • Kim, Dong-Soo;Han, Won-Jeong;Kim, Eun-Kyung
    • Imaging Science in Dentistry
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    • v.40 no.2
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    • pp.63-68
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    • 2010
  • Purpose : To compare the CT numbers on 3 cone-beam CT (CBCT) images with those on multi-detector CT (MDCT) image using CT phantom and to develop linear regressive equations using CT numbers to material density for all the CT scanner each. Materials and Methods : Mini CT phantom comprised of five 1 inch thick cylindrical models with 1.125 inches diameter of materials with different densities (polyethylene, polystyrene, plastic water, nylon and acrylic) was used. It was scanned in 3 CBCTs (i-CAT, Alphard VEGA, Implagraphy SC) and 1 MDCT (Somatom Emotion). The images were saved as DICOM format and CT numbers were measured using OnDemand 3D. CT numbers obtained from CBCTs and MDCT images were compared and linear regression analysis was performed for the density, $\rho$ ($g/cm^3$), as the dependent variable in terms of the CT numbers obtained from CBCTs and MDCT images. Results : CT numbers on i-CAT and Implagraphy CBCT images were smaller than those on Somatom Emotion MDCT image (p<0.05). Linear relationship on a range of materials used for this study were $\rho$=0.001H+1.07 with $R^2$ value of 0.999 for Somatom Emotion, $\rho$=0.002H+1.09 with $R^2$ value of 0.991 for Alphard VEGA, $\rho$=0.001H+1.43 with $R^2$ value of 0.980 for i-CAT and $\rho$=0.001H+1.30 with $R^2$ value of 0.975 for Implagraphy. Conclusion: CT numbers on i-CAT and Implagraphy CBCT images were not same as those on Somatom Emotion MDCT image. The linear regressive equations to determine the density from the CT numbers with very high correlation coefficient were obtained on three CBCT and MDCT scan.

Radiographic Diagnosis of Esophageal Obstruction by Persistent Right Aortic Arch in a Kitten (어린 고양이에서 우대동맥궁잔존에 의한 식도폐색의 방사선학적 진단)

  • 이기창;이희천;정성목;권오경;최민철
    • Journal of Veterinary Clinics
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    • v.20 no.2
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    • pp.248-251
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    • 2003
  • Esophageal obstruction as a result of persistence of the right aortic arch was diagnosed in a 3-month-old male Persian cat. Regurgitation right after weaning and retardation were noted in this cat. Survey radiographic signs on the lateral view include ventral deviation of the thoracic trachea caused by draping of the dilated esophagus over the dorsum of the trachea and a distinct interface of the dorsal wall of the esophagus silhouetting with the cranial thoracic hypaxial muscles. On the ventrodorsal view, the cranial mediastinum was widened with soft tissue density. The trachea was deviated to the right. In an esophagram, the segmental dilation of the esophagus with constriction of the lumen just cranial to the heart base was apparent. Thoracotomy was performed and corrective surgery was carried out. At surgery, it was confirmed that the esophagus was constricted at the cranial to the base of the heart.

Acquired myasthenia gravis in a domestic shorthair cat with cranial mediastinal mass

  • Song, Doo-Won;Kang, Min-Hee;Park, Hee-Myung
    • Korean Journal of Veterinary Research
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    • v.56 no.2
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    • pp.121-123
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    • 2016
  • A 7-year-old castrated male domestic short-hair cat presented with anorexia, constipation, depression, and voice alteration. Physical and neurological examinations revealed hyperthermia ($40.5^{\circ}C$), ventroflexion of the neck, reduced responses to external stimuli, generalized muscle weakness, and exercise intolerance. Thoracic radiographs revealed the presence of a cranial mediastinal mass. The history, clinical signs, and other examination results were compatible with acquired myasthenia gravis (MG). Acetylcholine receptor (AChR) antibody titers were determined to confirm MG and the serum AChR antibody concentration was 1.24 nmol/L (reference interval, < 0.3 nmol/L). This is the first diagnosis of acquired MG in a cat in Korea.

Double-chambered Right Ventricle in a Cat

  • Won, Sung-jun;Yoon, Junghee
    • Journal of Veterinary Clinics
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    • v.33 no.5
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    • pp.304-306
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    • 2016
  • A 12-month-old, 3.0-kg, male, Scottish fold cat was referred to Irion animal hospital with dyspnea and cardiac murmur. In radiograph, right-sided cardiomegaly and pleural effusion were identified. In echocardiographic examination, abnormal stenotic region was identified in right ventricle and dilation of right atrium and concentric hypertrophy of interventricular septum and the free wall of right ventricle were also identified. In the stenotic region, abnormal muscular band was identified. The turbulent flow was identified in infundibular region of right ventricular outflow tract with color-Doppler examination and high velocity flow (4.0 m/s) was also identified in stenotic region. With these results, double-chambered right ventricle (DCRV) was diagnosed.

The mechanism of sphingosine-1-phosphate induced contraction in cat esophageal smooth muscle cells.

  • Choi, Tae-Sik;Lee, Tai-Sang;Woo, Jae-Gwang;Kim, Yong-Sung;Sohn, Uy-Dong
    • Proceedings of the PSK Conference
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    • 2003.10b
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    • pp.77.3-78
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    • 2003
  • We previously shown that sphingosylphosphorylcholine, a lysophosphatidic acid, produced contraction in isolated single cells of cat ilium. We investigated the mechanism of sphingosine-1-phosphate (S1P)-induced contraction of circular smooth muscle cells in cat esophagus. S1P produced esophageal contraction in a dose dependent manner. The maximal contraction (l0$\^$-7/ M) induced at 1min. Pertusis toxin (PTX) inhibited contraction induced by S1P, suggesting that the contraction is mediated to a PTX-sensitive G-protein. (omitted)

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Streptomycetes Inducible Gene Cluster Involved in Aromatic Compound Metabolism

  • Park, Hyeon-Ju;Kim, Eung-Su
    • 한국생물공학회:학술대회논문집
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    • 2003.04a
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    • pp.422-427
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    • 2003
  • Streptomyces setonii (ATCC 39116) is a Gram-positive thermophilic soil actinomycetes capable of degrading single aromatic compounds including phenol and benzoate via ortho-cleavage pathway. we isolated approximately 6.3-kb S. setonii DNA fragment containing a thermophilic catechol 1,2-dioxygenase(C12O) gene. Here we further revealed that the 6.3-kb S. setonii DNA fragment was organized into two putative divergently-transcribed clusters with 6 complete and one incomplete open reading frames (ORFs). The first cluster with 3 ORFs showed significant homologies to previously known benA, benB, and benC, implying a part of benzoate catabolic operon. The second cluster revealed an ortho-cleavage catechol catabolic operon with three translationally-coupled ORFs (catR, catB, catA). Each of these individually-cloned ORFs was expressed in E. coli and identified as a distinct protein band with a theoretical molecular weight in SDS-PAGE. The expression of the cloned S. setonii catechol operon was induced in a heterologous S. lividans by specific single aromatic compounds including catechol, phenol, and 4-chlorophenol. The simitar induction pattern was also observed using a luciferase gene-fused reporter system, implying that S. setonii employs an inducer-specific regulatory mechanism for aromatic compound metabolism.

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Effects of Chemical Carcinogens on the Aldehyde Metabolic Enzymes and Antioxidant Enzymes in Clone 9 Cell (화학적 발암원이 Clone 9 세포의 알데히드대사 효소와 항산화 효소에 미치는 영향)

  • 김현배;염영나;이미영
    • Toxicological Research
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    • v.18 no.3
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    • pp.293-300
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    • 2002
  • Chemical carcinogen-induced alteration of aldehyde metabolic enzymes were examined in clone 9 cell. Diethylnitrosamine (DENA), N-nitrosoethylurea (NEU) and N-nitrosomorpholine (NNM) were wed as model carcinogens. Changes in enzyme activities by repetitive treatment of DENA, NEU or NNM were analyzed in terms of specific activities and activity stainings of the enzymes on the gel. Upon treatment of DENA, lipid peroxide level increased upto 10 fold, indicating strong oxidative stress state of the cell. Notable enhancement of ADH and ALDH activity occurred after DENA treatment, while glutathione-S-transferase activity was slightly increased. Furthermore, about 2.5 fold higher superoxide dismutase (SOD) activity was detected during deactivation of catalase (CAT) activity by repetitive treatment of DENA. However in NEU-treated cell, about 2.3 fold higher ALDH activity was found while ADH activity was slightly increased. Notable increase CAT and SOD could also be found. In contrast, maximum 3.5 fold higher CAT activity occurred during SOD deactivation in NNM-treated cell. These results suggest that there might be different enzymatic responses in relation to cell protection against DENA, NEU or NNM.

Per-Endoscopic Trans-Tympanic Traction Treatment of an Aural Inflammatory Polyp in a Cat

  • Park, Won-Keun;Song, Doo-Won;Shin, Dong-Hoon;Kim, So-Yeon;Lee, Ga-Won;Kang, Dong-Jae;Ro, Woong-Bin;Cho, Jong-Mun;Park, Hee-Myung
    • Journal of Veterinary Clinics
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    • v.38 no.3
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    • pp.147-151
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    • 2021
  • A 5-year-old castrated male Maine Coon cat presented with chronic otitis on the right ear. The clinical signs, scratching and head shaking, were recurrent despite symptomatic treatment, including antibiotics and antifungal agents. Video otoscopic examination and computed tomography (CT) examination revealed a polyp in the right ear canal. The polyp was removed via per-endoscopic trans-tympanic traction (PTT) with a grasping forceps. After treatment, Horner's syndrome occurred in the right eye but spontaneously resolved a week later. The mass was diagnosed as inflammatory polyp with a fibrovascular stroma containing plasma cells, lymphocytes, and neutrophils. All clinical signs were improved, and no recurrence was observed after 3 weeks of follow-up. This case report demonstrates that CT scan is effective in identifying the margin of polyp and PTT technique is non-invasive method for treating inflammatory polyp in cats without severe complications.