• Title/Summary/Keyword: CAR-T

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Unleashing the Therapeutic Potential of CAR-T Cell Therapy Using Gene-Editing Technologies

  • Jung, In-Young;Lee, Jungmin
    • Molecules and Cells
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    • v.41 no.8
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    • pp.717-723
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    • 2018
  • Chimeric antigen receptor (CAR) T-cell therapy, an emerging immunotherapy, has demonstrated promising clinical results in hematological malignancies including B-cell malignancies. However, accessibility to this transformative medicine is highly limited due to the complex process of manufacturing, limited options for target antigens, and insufficient anti-tumor responses against solid tumors. Advances in gene-editing technologies, such as the development of Zinc Finger Nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9), have provided novel engineering strategies to address these limitations. Development of next-generation CAR-T cells using gene-editing technologies would enhance the therapeutic potential of CAR-T cell treatment for both hematologic and solid tumors. Here we summarize the unmet medical needs of current CAR-T cell therapies and gene-editing strategies to resolve these challenges as well as safety concerns of gene-edited CAR-T therapies.

T0901317 as an Inhibitor of Transcriptional Activation of Constitutive Androstane Receptor (CAR) (Constitutive androstane receptor (CAR)의 전사활성 저해제로서의 T0901317)

  • Kim, Hyun-Ha;Seol, Won-Gi
    • Journal of Life Science
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    • v.21 no.4
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    • pp.481-485
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    • 2011
  • T0901317 is a potent synthetic ligand for liver X receptor (LXR, NR1H2/3), a member of the nuclear receptor superfamily that functions as a transcription factor. However, T0901317 has been also reported to modulate the activity at least four other nuclear receptors (NRs), acting as agonists for farnesoid X receptor (FXR, NR1H4) and pregnane X receptor (PXR, NR1I2) and as antagonists for androgen receptor (AR, NR3C4) and retinoid-related orphan receptor-${\alpha}$ (ROR-${\alpha}$, NR1F1). We report here that T0901317 can also function as an inhibitor for constitutive androstane receptor (CAR, NR1I3). Since CAR is a major player of xenobiotic and cholesterol metabolism in the liver, along with PXR, FXR and LXR, which are reported to be regulated by T0901317, this further complicates the interpretation of potential results with T0901317 in liver cells.

Smart Parking Guidance System based on IoT Car-stoppers (IoT 카스토퍼 기반 스마트 주차안내 시스템)

  • Shim, Dongha;Yang, Ji-Hoon;Son, Jeungki;Han, Seung-Han;Lee, Hyounmin
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.17 no.3
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    • pp.137-143
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    • 2017
  • This paper presents a smart parking guidance system based on IoT car-stoppers. The car-stopper embedding an IoT sensor module has the advantage of easy installation compared to conventional parking sensors buried in ground. The parking status data are transferred to the IoT gateway by the sequential point-to-point communication between the car-stoppers. The data transferred from the IoT gateway are stored in the web server, and parking spaces can be monitored remotely through the Android app in a smart device. An active/sleep cycle method using a watch dog timer is employed to reduce the power consumption of the battery powered car-stopper. The power consumption of the car-stopper is measured to be 80 and 25 mW at the active and sleep mode, respectively. A configuration of ultra-low-power IoT sensor module is proposed to minimize the power consumption in the sleep mode. The operation of the implemented system has been verified in a real-world parking lot.

Current Perspectives on Emerging CAR-Treg Cell Therapy: Based on Treg Cell Therapy in Clinical Trials and the Recent Approval of CAR-T Cell Therapy (장기이식 거부반응과 자가면역질환 치료제로서의 CAR Treg 세포치료제의 가능성: Treg 세포치료제 임상시험 현황과 CAR T 세포치료제 허가 정보를 바탕으로)

  • Kang, Koeun;Chung, Junho;Yang, Jaeseok;Kim, Hyori
    • Korean Journal of Transplantation
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    • v.31 no.4
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    • pp.157-169
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    • 2017
  • Regulatory T cells (Treg) naturally rein in immune attacks, and they can inhibit rejection of transplanted organs and even reverse the progression of autoimmune diseases in mice. The initial safety trials of Treg against graft-versus-host disease (GVHD) provided evidence that the adoptive transfer of Treg is safe and capable of limiting disease progression. Supported by such evidence, numerous clinical trials have been actively investigating the efficacy of Treg targeting autoimmune diseases, type I diabetes, and organ transplant rejection, including kidney and liver. The limited quantity of Treg cells harvested from peripheral blood and subsequent in vitro culture have posed a great challenge to large-scale clinical application of Treg; nevertheless, the concept of CAR (chimeric antigen receptor)-Treg has emerged as a potential resolution to the problem. Recently, two CAR-T therapies, tisagenlecleucel and axicabtagene ciloleucel, were approved by the US FDA for the treatment of refractory or recurrent acute lymhoblastic leukemia. This approval could serve as a guideline for the production protocols for other genetically engineered T cells for clinical use as well. The phase I and II clinical trials of these agents has demonstrated that genetically engineered and antigen-targeting T cells are safe and efficacious in humans. In conclusion, both the promising results of Treg cell therapy from the clinical studies and the recent FDA approval of CAR-T therapies are paving the way for CAR-Treg therapy in clinical use.

Effects of Supplementary Blood Meal on Carnosine Content in the Breast Meat and Laying Performance of Old Hens

  • Namgung, N.;Shin, D.H.;Park, S.W.;Paik, I.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.7
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    • pp.946-951
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    • 2010
  • The objective of this research was to evaluate the effects of dietary supplementation of blood meal (BM) as a source of histidine, and magnesium oxide (MgO) as a catalyst of carnosine synthetase, on carnosine (L-Car) content in the chicken breast muscle (CBM), laying performance, and egg quality of spent old hens. Four hundred eighty laying hens (Hy-Line$^{(R)}$ Brown), 95wk old, were allotted randomly into five replicates of six dietary treatments: T1; 100% basal diet, T2; 100% basal diet+MgO, T3; 97.5% basal diet+2.5% BM, T4; 97.5% basal diet+2.5% BM+MgO, T5; 95% basal diet+5% BM, T6; 95% basal diet+5% BM+MgO. Magnesium oxide was added at 0.3% of diets. The layers were fed experimental diets for 5wk. There were no significant differences in the weekly L-Car content in CBM among all treatments during the total experimental period, but some of the contrast comparisions showed higher L-Car in CBM of T6. The L-Car contents linearly decreased (p<0.01 or p<0.05) as the layers got older except in T4 (p>0.05). There were significant differences in egg weight (p<0.01) and soft and broken egg ratio (p<0.05). The control (T1) was highest in egg weight and T6 was lowest in soft and broken egg ratio. Among the parameters of egg quality, there were significant differences in eggshell strength (p<0.01) and egg yolk color (p<0.05). Magnesium oxide supplementation increased the eggshell strength and BM tended to decrease egg yolk color. Eggshell color, eggshell thickness, and Haugh unit were not influenced by BM and MgO. In conclusion, BM and MgO did not significantly influence the L-Car in CBM of spent layers. The L-Car content rapidly decreased as the layers became senescent. Eggshell strength was increased by MgO supplementation.

Carvacrol improves blood lipid and glucose in rats with type 2 diabetes mellitus by regulating short-chain fatty acids and the GPR41/43 pathway

  • Yan Sun;Hai Qu;Xiaohong Niu;Ting Li;Lijuan Wang;Hairui Peng
    • The Korean Journal of Physiology and Pharmacology
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    • v.28 no.1
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    • pp.1-10
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    • 2024
  • Type 2 diabetes mellitus (T2DM) is characterized by hyperglycemia and dyslipidemia. Carvacrol (CAR) has demonstrated the potential to mitigate dyslipidemia. This study aims to investigate whether CAR can modulate blood glucose and lipid levels in a T2DM rat model by regulating short-chain fatty acids (SCFAs) and the GPR41/43 pathway. The T2DM rat model was induced by a high-fat diet combined with low-dose streptozocin injection and treated with oral CAR and/or mixed antibiotics. Fasting blood glucose, oral glucose tolerance, and insulin tolerance tests were assessed. Serum lipid parameters, hepatic and renal function indicators, tissue morphology, and SCFAs were measured. In vitro, high glucose (HG)-induced IEC-6 cells were treated with CAR, and optimal CAR concentration was determined. HG-induced IEC-6 cells were treated with SCFAs or/and GPR41/43 agonists. CAR significantly reduced blood lipid and glucose levels, improved tissue damage, and increased SCFA levels in feces and GPR41/43 expression in colonic tissues of T2DM rats. CAR also attenuated HG-induced apoptosis of IEC-6 cells and enhanced GPR41/43 expression. Overall, these findings suggest that CAR alleviates blood lipid and glucose abnormalities in T2DM rats by modulating SCFAs and the GPR41/43 pathway.

A study on detection methodology of threat on cars from the viewpoint of IoT (IoT 관점에서의 차량 위협 탐지 방안)

  • Kwak, Byung Il;Han, Mi Ran;Kang, Ah Reum;Kim, Huy Kang
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.25 no.2
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    • pp.411-421
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    • 2015
  • These days, a conversion of the fast-advancing ICT (Information and Communications Technologies) and the IoT (Internet of Things) has been in progress. However, these conversion Technology could lead to many of the security threat existing in the ICT environment. The security threats of car in the IoT environment could cause the property damage and casualty. There are the inadequate preparations for the car security and the difficulty of detection for the security threats by itself. In this paper, we proposed the decision-making framework for the anomaly detection and found out what are the threats of car in the IoT environment. The discrimination of the factor, path and type of threats from the attack against the car should take priority over the self-inspection and the swift handling of the attack on control system.

Mirror vision for car Black box (자동차 블랙박스를 위한 미러 비전)

  • Kim, Eun-Ho;Lim, Myoung-Sub
    • Proceedings of the KIEE Conference
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    • 2007.04a
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    • pp.369-372
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    • 2007
  • about commercial business of car black box, mirror vision for car black box deal with analysis of Existing Car Black boxes in market to provide the objective information associated with surrounding scene of car instead of witness, we experimented on suitable structure of all direction to cover surrounding of car considering dead zone where can't see at short distance and realized simple structure of gathering scene using mirror and lens and by saving the number of camera and MUX of pre-circut

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Study for Support Structure of Liftable Car Deck on PCTC (자동차 운반선 이동식 갑판의 Latch 보강 적정설계 연구)

  • Na, Yongmoon;Chae, Wooki
    • Special Issue of the Society of Naval Architects of Korea
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    • 2013.12a
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    • pp.60-65
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    • 2013
  • Now days, the demands of new type hull lines and optimum design in relation with the EEDI (Energy Efficiency Design Index) regulation and eco-friendly high efficiency vessel design are mandatory clauses in Euro financial crises era. Therefore, in correlation with the above, we tried to find the optimum results and revealed the alterations of supporting structure for liftable car deck latch on PCTC. Generally, PCTC (Pure Car & Truck Carrier) design has been performed by 2 pillar space model F. E analysis without vehicle loads on liftable car deck to evaluate the structural adaptability. So, we applied mentioned vehicle loads on pillar and side transverse web on model to compare with not applied model and performed the ultimate strength analysis of improved design for the safety evaluation.

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A Study on the Solar Cell Charging Equipment for Hybrid Vehicle (Hybrid 자동차용 Solar Cell 충전장치에 관한 연구)

  • Kim, G.S.;Park, S.C.
    • Journal of the Korean Society of Mechanical Technology
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    • v.13 no.4
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    • pp.157-164
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    • 2011
  • Hybrid car can improve fuel efficiency using a power of motor that is generated during constant-speed or deceleration driving. The motor is located between engine and transmission. But, when voltage of main battery is low, fuel efficiency is low because the voltage can't run the motor. In this situation, this study observed fuel efficiency when using solar cell for assistance power. In order to verify a fuel consumption of hybrid car equipped solar cell for assistance power, the car was tested downtown driving. As hybrid car was equipped solar cell for assistance, fuel consumption was reduced 8.35 % at running air conditioner. And, at air conditioner doesn't work, fuel consumption was reduced 6.88 %. This point of view, CO2 is expected to reduce in similar proportion.