• Journal of Animal Science and Technology
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• 제65권2호
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• pp.473-477
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• 2023

Lactobacillus amylovorus CACC736 was originated from swine feces in Korea. The complete genome sequences of the strain contained one circular chromosome (2,057,809 base pair [bp]) with 38.2% guanine-cytosine (GC) content and two circular plasmids, namely, pCACC736-1 and pCACC736-2. The predicted protein-coding genes, which are encoding the clustered regularly interspaced short palindromic repeats (CRISPR)-associated proteins, biosynthesis of bacteriocin (helveticin J), and the related proteins of the bile, acid tolerance. Notably, the genes related to vitamin B-group biosynthesis (riboflavin and cobalamin) were also found in L. amylovorus CACC736. Collectively, the complete genome sequence of the L. amylovorus CACC736 will aid in the development of functional probiotics in the animal industry.

• Journal of Animal Science and Technology
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• 제65권5호
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• pp.1105-1109
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• 2023

Pedi coccus acidilactici CACC 537 was isolated from canine feces and reported to have probiotic properties. We aimed to characterize the potential probiotic properties of this strain by functional genomic analysis. Complete genome sequencing of P. acidilactici CACC 537 was performed using a PacBio RSII and Illumina platform, and contained one circular chromosome (2.0 Mb) with a 42% G + C content. The sequences were annotation revealed 1,897 protein-coding sequences, 15 rRNAs, and 56 tRNAs. It was determined that P. acidilactici CACC 537 genome carries genes known to be involved in the immune system, defense mechanisms, restriction-modification (R-M), and the CRISPR system. CACC 537 was shown to be beneficial in preventing pathogen infection during the fermentation process, help host immunity, and maintain intestinal health. These results provide for a comprehensive understanding of P. acidilactici and the development of industrial probiotic feed additives that can help improve host immunity and intestinal health.

• Journal of Animal Science and Technology
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• 제63권6호
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• pp.1464-1467
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• 2021

Bacillus coagulans CACC 834 was isolated from canine feces, and its potential probiotic properties were characterized by functional genome analysis. Whole-genome sequencing of B. coagulans CACC 834 was performed using the PacBio RSII platforms. The complete genome assembly consisted of one circular chromosome (3.1 Mb) with guanine (G) + cytosine (C) content of 47.1%. Annotation revealed 3,181 protein-coding sequences (CDSs), 30 rRNAs, and 83 tRNAs. Gene associated 11% of the genes were involved in replication, recombination, and repair. We also annotated various stress-related, acid resistance, bile salt resistance and adhesion-related domains in this strain, which likely provide support in exerting probiotic action by survival under gastrointestinal tract. These results add to our comprehensive understanding of B. coagulans and suggest potential mammal-related industrial applications.

• Journal of Animal Science and Technology
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• 제62권6호
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• pp.952-955
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• 2020

Bacteroides sp. CACC 737 was isolated from a feline, and its potential probiotic properties were characterized using functional genome analysis. Whole-genome sequencing was performed using the PacBio RSII and Illumina HiSeq platforms. The complete genome of strain CACC 737 contained 4.6 Mb, with a guanine (G) + cytosine (C) content of 45.8%, six cryptic plasmids, and extracellular polysaccharide gene as unique features. The strain was beneficial to animal health when consumed as feed, for example, for ameliorating immunological dysfunctions and metabolic disorders. The genome information adds to the comprehensive understanding of Bacteroides sp. and suggests potential animal-related industrial applications for this strain.

• 교통기술과정책
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• 제13권4호
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• pp.30-41
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• 2016

• BMB Reports
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• 제50권3호
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• pp.109-110
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• 2017

The nature of encoded information in neural circuits is determined by neuronal firing patterns and frequencies. This paper discusses the molecular identity and cellular mechanisms of spike-frequency adaptation in the central nervous system (CNS). Spike-frequency adaptation in thalamocortical (TC) and CA1 hippocampal neurons is mediated by the $Ca^{2+}$-activated $Cl^-$ channel (CACC) anoctamin-2 (ANO2). Knockdown of ANO2 in these neurons results in increased number of spikes, in conjunction with significantly reduced spike-frequency adaptation. No study has so far demonstrated that CACCs mediate afterhyperpolarization currents, which result in the modulation of neuronal spike patterns in the CNS. Our study therefore proposes a novel role for ANO2 in spike-frequency adaptation and transmission of information in the brain.

• Asian-Australasian Journal of Animal Sciences
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• 제16권7호
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• pp.1055-1059
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• 2003

Keratins, the constituents of epithelial intermediate filaments, are precisely regulated in a tissue and development specific manner. There are two types of keratin in bovine. The type I is acidic keratin and the type II is neutral/basic keratin. 1.5 kb of 5' flanking sequence of Korean cattle Keratin IV gene, type II keratin (59 kDa), was cloned and sequenced. A symmetrical motif AApuCCAAA are located in a defined region upstream of the TATA box. Proximal SP1, AP1, E-box and CACC elements as the major determinants of transcription are identified. When it was compared to the bovine sequence from -600 bp to ATG upstream, the homology was 97% in nucleotide sequence. Several A and T sequences, located in the promoter region, are deleted in the Korean cattle. An expression vector consisted of Korean cattle Keratin IV gene promoter/SV40 large T antigen was transfected to HaCaT cell (Epithelial keratinocyte). The transformed HaCaT cells showed active proliferation when treated with PDGF (Platelet-derived growth factor) in 0.3% soft agar compared to control cells. These results indicate that Korean cattle Keratin IVgene promoter can be used as a promoter for transfection into epithelial cell.

• 한국ITS학회 논문지
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• 제16권2호
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• pp.55-72
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• 2017

자율주행차량은 다양한 사회 문제를 해결하기 위한 대안으로 각광받고 있다. 자율주행차량의 도입 및 관련 기술 개발 연구가 활발히 진행되고 있으나 자율주행차량에 탑재된 운행 알고리즘에 따른 실제 운행의 영향력에 대한 연구는 미흡한 실정이다. 본 연구에서는 한정된 물리적 환경에서 공리주의와 사회적 합의를 고려하여 차량 운행 중 발생 가능한 상황인 중앙선 침범 상황에 대한 시나리오를 설정하고 시뮬레이션을 통해 윤리적 판단이 필요한 상황에서의 자율주행차량의 운행에 따른 영향을 분석하였다. 분석 결과, 자율주행차량이 안전도를 판단하여 옆 차선으로 차선을 변경한 시나리오에서 사고에 따른 영향이 더 큰 것으로 나타났다. 또한 자율주행차량의 판단에 따라 전방 충돌한 경우, 자율주행차량의 기능에 의해 교통류 개선 효과가 있음을 확인하였다.

• 한국ITS학회 논문지
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• 제19권5호
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• pp.52-69
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• 2020

화물차 군집주행이 화물교통량 증가에 따른 문제를 해결할 수 있는 대안으로 떠오르는 가운데, 화물차 군집주행이 교통류에 미치는 영향과 군집주행 전용차로 도입 효과를 분석하는 연구를 수행하였다. 연구결과, 교통량이 많고 화물차 혼입률이 높아질수록 화물차의 군집비율이 높아지고 최대 군집형성 규모가 증가함에 따라 군집주행으로 인한 평균속도 증가효과가 더 크게 나타났다. 하지만, 진출입 등으로 대기행렬이 발생한 경우에는 평균속도 증가효과는 기대하기 어려우며 오히려 처리량이 감소할 수 있는 것으로 분석되었으므로 접속부의 용량 확보 등 접속도로와 연계된 교통운영 전략이 필요하다는 점을 확인할 수 있었다. 군집 전용차로의 효과는 자율주행화물차 비율(MPR)이 100%가 되어 한 개 차로가 완전히 자율주행화물차만으로 운영될 경우에만 미 도입 시 보다 긍정적 효과를 얻을 수 있었다. 본 연구는 국내 도로·교통 조건을 시나리오에 반영하여 군집주행의 영향을 종합적으로 분석하였으므로 구간별, 시간대별로 군집주행 및 전용차로의 영향을 가늠해 볼 수 있으며, 이는 향후 화물차 군집주행을 고려한 도로 운영전략 수립이나 관리에 기초자료로 사용할 수 있을 것으로 판단된다.