• Journal of Animal Science and Technology
• /
• v.65 no.2
• /
• pp.473-477
• /
• 2023

Lactobacillus amylovorus CACC736 was originated from swine feces in Korea. The complete genome sequences of the strain contained one circular chromosome (2,057,809 base pair [bp]) with 38.2% guanine-cytosine (GC) content and two circular plasmids, namely, pCACC736-1 and pCACC736-2. The predicted protein-coding genes, which are encoding the clustered regularly interspaced short palindromic repeats (CRISPR)-associated proteins, biosynthesis of bacteriocin (helveticin J), and the related proteins of the bile, acid tolerance. Notably, the genes related to vitamin B-group biosynthesis (riboflavin and cobalamin) were also found in L. amylovorus CACC736. Collectively, the complete genome sequence of the L. amylovorus CACC736 will aid in the development of functional probiotics in the animal industry.

• Journal of Animal Science and Technology
• /
• v.65 no.5
• /
• pp.1105-1109
• /
• 2023

Pedi coccus acidilactici CACC 537 was isolated from canine feces and reported to have probiotic properties. We aimed to characterize the potential probiotic properties of this strain by functional genomic analysis. Complete genome sequencing of P. acidilactici CACC 537 was performed using a PacBio RSII and Illumina platform, and contained one circular chromosome (2.0 Mb) with a 42% G + C content. The sequences were annotation revealed 1,897 protein-coding sequences, 15 rRNAs, and 56 tRNAs. It was determined that P. acidilactici CACC 537 genome carries genes known to be involved in the immune system, defense mechanisms, restriction-modification (R-M), and the CRISPR system. CACC 537 was shown to be beneficial in preventing pathogen infection during the fermentation process, help host immunity, and maintain intestinal health. These results provide for a comprehensive understanding of P. acidilactici and the development of industrial probiotic feed additives that can help improve host immunity and intestinal health.

• Journal of Animal Science and Technology
• /
• v.63 no.6
• /
• pp.1464-1467
• /
• 2021

Bacillus coagulans CACC 834 was isolated from canine feces, and its potential probiotic properties were characterized by functional genome analysis. Whole-genome sequencing of B. coagulans CACC 834 was performed using the PacBio RSII platforms. The complete genome assembly consisted of one circular chromosome (3.1 Mb) with guanine (G) + cytosine (C) content of 47.1%. Annotation revealed 3,181 protein-coding sequences (CDSs), 30 rRNAs, and 83 tRNAs. Gene associated 11% of the genes were involved in replication, recombination, and repair. We also annotated various stress-related, acid resistance, bile salt resistance and adhesion-related domains in this strain, which likely provide support in exerting probiotic action by survival under gastrointestinal tract. These results add to our comprehensive understanding of B. coagulans and suggest potential mammal-related industrial applications.

• Journal of Animal Science and Technology
• /
• v.62 no.6
• /
• pp.952-955
• /
• 2020

Bacteroides sp. CACC 737 was isolated from a feline, and its potential probiotic properties were characterized using functional genome analysis. Whole-genome sequencing was performed using the PacBio RSII and Illumina HiSeq platforms. The complete genome of strain CACC 737 contained 4.6 Mb, with a guanine (G) + cytosine (C) content of 45.8%, six cryptic plasmids, and extracellular polysaccharide gene as unique features. The strain was beneficial to animal health when consumed as feed, for example, for ameliorating immunological dysfunctions and metabolic disorders. The genome information adds to the comprehensive understanding of Bacteroides sp. and suggests potential animal-related industrial applications for this strain.

• Transportation Technology and Policy
• /
• v.13 no.4
• /
• pp.30-41
• /
• 2016

• BMB Reports
• /
• v.50 no.3
• /
• pp.109-110
• /
• 2017

The nature of encoded information in neural circuits is determined by neuronal firing patterns and frequencies. This paper discusses the molecular identity and cellular mechanisms of spike-frequency adaptation in the central nervous system (CNS). Spike-frequency adaptation in thalamocortical (TC) and CA1 hippocampal neurons is mediated by the $Ca^{2+}$-activated $Cl^-$ channel (CACC) anoctamin-2 (ANO2). Knockdown of ANO2 in these neurons results in increased number of spikes, in conjunction with significantly reduced spike-frequency adaptation. No study has so far demonstrated that CACCs mediate afterhyperpolarization currents, which result in the modulation of neuronal spike patterns in the CNS. Our study therefore proposes a novel role for ANO2 in spike-frequency adaptation and transmission of information in the brain.

• Asian-Australasian Journal of Animal Sciences
• /
• v.16 no.7
• /
• pp.1055-1059
• /
• 2003

Keratins, the constituents of epithelial intermediate filaments, are precisely regulated in a tissue and development specific manner. There are two types of keratin in bovine. The type I is acidic keratin and the type II is neutral/basic keratin. 1.5 kb of 5' flanking sequence of Korean cattle Keratin IV gene, type II keratin (59 kDa), was cloned and sequenced. A symmetrical motif AApuCCAAA are located in a defined region upstream of the TATA box. Proximal SP1, AP1, E-box and CACC elements as the major determinants of transcription are identified. When it was compared to the bovine sequence from -600 bp to ATG upstream, the homology was 97% in nucleotide sequence. Several A and T sequences, located in the promoter region, are deleted in the Korean cattle. An expression vector consisted of Korean cattle Keratin IV gene promoter/SV40 large T antigen was transfected to HaCaT cell (Epithelial keratinocyte). The transformed HaCaT cells showed active proliferation when treated with PDGF (Platelet-derived growth factor) in 0.3% soft agar compared to control cells. These results indicate that Korean cattle Keratin IVgene promoter can be used as a promoter for transfection into epithelial cell.

• The Journal of The Korea Institute of Intelligent Transport Systems
• /
• v.16 no.2
• /
• pp.55-72
• /
• 2017

Autonomous Vehicles(AV) are considered as an alternative to solve various social problems. Many researches which are related to developing technologies and AV operations have been conducted vastly and on-going. However, there seem to be little studies on various influences of AI algorithm on driving installed in AV. This study aims to examine the impacts of the ethical decisions made by Utilitarianism-based AI in AV when the oncoming car crossed over the central line. It establishes scenarios about situation of encroaching a central line and analyzes traffic impacts of ethical decision made by AV. According to the results of the analyses, as th accident occurs, overall speed of traffic decrease. There is a negative impact on the traffic flow when AV made an Utilitarian-based ethical decision by changing the lane. However, when AV choose to collide head-on, there is a positive effect to relieve traffic flow with an assistance of CACC, equipped.

• The Journal of The Korea Institute of Intelligent Transport Systems
• /
• v.19 no.5
• /
• pp.52-69
• /
• 2020

Considering the need for an infrastructure-level review, this study analyzed the impact of truck platooning on freeway traffic flow and the effect of dedicated lanes based on domestic road and traffic conditions. According to the study, the higher traffic volume and truck ratio, the higher ratio of platoons and the greater size of platoons are formed, which results in greater effect of increasing the average speed of the network. Therefore, the routes with heavy traffic and heavy cargo traffic could be positively considered for truck platooning. However, the analysis showed that the effect of increasing the average speed of the entire network is difficult to expect in the event of a queue due to entry and exit, and that the overall network's throughput could be reduced. Therefore, traffic operation strategies associated with the access road, such as securing capacity of the connection, are needed to maximize the effect of truck platooning. When it comes to the effect of dedicated lane, it could have a positive effect only if one lane was fully operated by automated trucks under the condition of 100% MPR, which allowed positive effects in all aspects, such as higher average speed, throughput, and reduced conflict rates.