• Title/Summary/Keyword: CA association

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Role of Calcium and Calcium Channels in Progesterone Induced Acrosome Reaction in Caprine Spermatozoa

  • Somanath, P.R.;Gandhi, K.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.7
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    • pp.949-956
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    • 2002
  • There are several physiological and pharmacological evidences indicating that opening of voltage dependent $Ca^{2+}$ channels play a critical role in induction of acrosome reaction in mammalian sperm. We determined the intracellular free $Ca^{2+}$ concentration in ejaculated goat sperm using a fluorescent, $Ca^{2+}$-specific probe, Fura2/AM, after the suspension of sperm in KRB medium, capable of sustaining capacitation and the acrosome reaction. We used nifedipine, D-600 and diltiazem, the $Ca^{2+}$ channel antagonists belonging to the classes of dihydropyridines, phenylalkylamines and benzothiazepines, to investigate the possibility that L-type voltage gated $Ca^{2+}$ channels play a role in the progesterone-stimulated exocytotic response. Progesterone promoted a rise in intracellular $Ca^{2+}$ in goat sperm and addition of nifedipine (100 nM) just prior to progesterone induction, significantly inhibited both intracellular $Ca^{2+}$ rise and exocytosis suggesting that $Ca^{2+}$ channels are involved in the process. However, the intracellular $Ca^{2+}$ increase during the process of capacitation was not affected with the addition of nifedipine suggesting a role of focal channel for $Ca^{2+}$ during capacitation. Studies using monensin and nigericin, two monovalent cation ionophores showed that an influx of $Na^+$ also may play a role in the opening of $Ca^{2+}$ channels. These results strongly suggests that the entry of $Ca^{2+}$ channels with characteristics similar to those of L-type, voltage-sensitive $Ca^{2+}$ channels found in cardiac and skeletal muscle, is a crucial step in the sequence of events leading to progesterone induced acrosome reaction in goat sperm.

Design of ClientCA Operation and Mechanism for e-Commerce Certificate Management (전자거래 인증서 관리를 위한 ClientCA 운영 메커니즘 설계)

  • 김점구;나상엽
    • Proceedings of the Korea Information Assurance Society Conference
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    • 2004.05a
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    • pp.71-76
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    • 2004
  • A certificate is important media for the purpose of offering user-authentication service on PKI system. In the paper we analyzed management implement which could make the efficient use of a certificate. This implement called ClientCA will make efficient use of the service about user-authentication consisting of the basis in the age of information through efficient management and partial use of each certificates. Especially, ClientCA could be used efficiently by grafting a small group of PKI system which is operated with particular purposes.

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Preparation of $CaSO_4$ Nanoparticles by Catanionic Vesicles Formed in Cationic OTAC and Anionic ADS Mixed Aqueous Solution (양이온 OTAC와 음이온ADS 혼합 수용액에서 형성된 층막구형체에 의한 $CaSO_4$ 나노입자의 제조)

  • Kim, Hong-Un;Lim, Kyung-Hee
    • Journal of the Korean Applied Science and Technology
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    • v.21 no.4
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    • pp.380-387
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    • 2004
  • The preparation of $CaSO_4$ nanoparticle by vesicles formed spontaneously in cationic OTAC and anionic ADS mixed surfactant solution whose ratio is 0.3/0.7 is investigated. Added electrolytes for preparing nanoparticles reduce vesicle size about 200-300 nm comparing with that of pure vesicle whose size is 700-800 nm by DLS. The core of vesicles has 200 nm size and acts as nanoreactors which same size of monodisperse $CaSO_4$ nanopaticles are formed. Although $CaSO_4$ particles are formed at the outer of vesicles, they are very large and amorphous. The formed particles are identified with XRD analysis after separation due to coinciding with $CaSO_4$ particles.

The behaviour of the internal bubbles in $CaF_{2}$ crystals during the annealing process ($CaF_{2}$ 결정의 annealing시 내부 bubbles의 거동)

  • Shim, Kwang-Bo;Park, Dai-Chul;Joo, Kyoung;Auh, Keun-Ho
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.6 no.4
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    • pp.595-599
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    • 1996
  • The behaviour of the internal bubbles present in $CaF_{2}$ crystals was characterized crystallographically using a variety of the mircroscopical technique. The bubble defects were found to be aligned on the characteristic planes and directions depending on the crystals structure of the $CaF_{2}$. The AFM analysis revealed that these behaviors are related to the S-surface formation by the negative grain growth mechanism.

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Pigment particle size distribution이 백판지 품질에 미치는 영향

  • 김진현;이도엽;최강영
    • Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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    • 2001.11a
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    • pp.55-56
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    • 2001
  • 지난 수년간 유럽을 중심으로 Carbonate의 PSD (Particle Size Distribution)가 도공지 의 백색도, 불투명도, Coverage등에 미치는 영향에 대하여 많은 연구가 진행되어 왔다. 현재 국내의 Coating Pigment System 역시 Carbonate 위주로 구성되어 있으나, 입자 분포에 따른 실증적 연구가 선진국에 비하여 부족한 상태이다. 본 연구는 최근 유럽에서 사용 중인 Narrow PSD Carbonate가 도공지 품질에 미치는 영향을 검토한 것으로서, 특히 Multiple Coating에서의 도공층 구조변화와 그에 따른 백지물성 및 인쇄적성의 변화를 연구한 것이다. 실험의 Case는 Broad PSD CaC03와 Narrow PSD CaC03를 비 교하였 다 .. Color F Formulation은 Pre Color에서 #1 Clay 30 p따t와 각각 CaC03 70 p따t를 사용하였다. 그리고 바인더와 첨가제는 동일하게 사용하였다 .. Top color Formulation 또한 동일하 게 사용하였다. 코팅방식은 speed 450rrνmin, pre rod coating, top blade coating 방식 으로 CLC (Cy lindrical Laboratory Coater)를 사용하여 코팅하였다. 백지품질은 백색도, 평활도 ,광택도, 거칠음도, Bum Out Test와 Coverage( SEM 사 진 Z -direction) 등을 측정하였다. 인쇄품질은 인쇄 광택도, 인쇄 Picking strength와 2 도 인쇄 농도를 측정하였다. 백색도는 Narrow PSD CaC03를 사용한 것이 Broad PSD CaC03를 사용한 것 대비 약1% 우수하였다. 이것은 Narrow PSD CaC03를 사용한 것이 filter cake이 많이 open 되 어 있어 dewatering이 빠르고, Wet void volume이 증가되 어 부동화점 의 농도가 낮 아져 원지 층으로 침 투되 는 pigment가 작고 표층에 잔류하는 pigment와 void volume의 증가로 인해 기공층이 증가한 것으로 판단된다. 그리고, 백지광택도, 거칠음도 등 또한 Narrow PSD CaC03를 사용한 것이 우수하였 다. 이는 Pre color에서 coverage의 개선으로 top color층의 도공량 uniformity가 개선 된 이유라고 생각한다. 그러나 인쇄품질은 백지와 비교하여 차이가 없었다. 이것은 top 칼라 Formulation이 동일하고, 인쇄시 잉크흡수 거동은 top color 층에 주로 영향을 받기 때문이라고 판단 된다. 향후에는 인쇄품질의 향상을 위하여 Case 2의 CaC03 100% 사용가능성 및 Pre Color 는 Case 2로 고정하고, Top칼라를 변경하는 방법을 또한 생각할 필요가 있다고 판단된다.

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Interactive effect of dietary levels of calcium and 25-hydroxy vitamin D3 on the performance, serum biochemical concentration and digestibility of laying hens from 61 to 70 weeks of age

  • Lim, Chun Ik;Ryu, Kyeong Seon
    • Animal Bioscience
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    • v.35 no.9
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    • pp.1426-1433
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    • 2022
  • Objective: The present research was conducted to evaluate the interactive effect of dietary concentration of calcium (Ca) and 25-hydroxy vitamin D3 (25OHD3) on the performance, blood composition and digestibility of laying hens. Methods: A total of 540 Hy-line brown laying hens aged 61 to 70 weeks were randomly allotted in a 3×3 factorial arrangement, consisting of three levels of 25OHD3 (0, 25, and 50 ㎍/kg) and three levels of Ca (3.5%, 4.0%, and 4.5%). All diets had basal concentration of 3,000 IU/kg of vitamin D3 including the 2,800 kcal/kg of metabolic energy and 16% of crude protein. Results: The results showed that interactive effect (p<0.05) between Ca and 25OHD3 was such that dietary 25OHD3 linearly increased interleukin-6 at all levels of Ca inclusion. Interaction (p<0.05) occurred with the highest parathyroid hormone in laying hens that received dietary concentration of Ca (3.5%) with 25OHD3 (50 ㎍/kg), and Ca (4.0%) with 25OHD3 (50 ㎍/kg). Egg production and egg weight significantly (p<0.05) increased in the 4.5% Ca group compared to the 3.5% to 4.0% Ca groups. Egg shell thickness and tibia bone length also increased (p<0.05) in groups fed a high-Ca diet (4.0% to 4.5%). Phosphorus digestibility significantly (p<0.05) increased along with dietary Ca level. Among the tested 25OHD3 groups, higher (p<0.05) egg production and tibia thickness were present in hens fed 50 ㎍/kg of 25OHD3. Furthermore, Ca digestibility serum Ca and 25OHD3 were significantly increased in group offered 50 ㎍/kg of 25OHD3. Conclusion: The results gathered in this study indicate that dietary concentrations of 4.0% to 4.5% Ca and 50 ㎍/kg 25OHD3 improve the performance of hens from 61 to 70 weeks of age.

Experimental Study on the Prevention of Periodontal Disease (치주질환 발생기전 및 예방에 관한 실험적 연구)

  • Choi, Keun-Bai
    • The Journal of the Korean dental association
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    • v.17 no.12 s.127
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    • pp.895-899
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    • 1979
  • 치석다침착자의 치석의 화학성분함량을 구명하기 위하여 치태제거후 6개월에 치태침착도가 심한 사람과 경한 사람을 분류하여 전악으로부터 치태를 채취하였다. 그리고 채취된 치태 세포성분획과 비세포성 부분으로 분리하여 각 분획에서 탄수화물, 단백질, 지질, Ca, Mg, K 및 P의 함량을 화학적으로 분석측정한 결과는 다음과 같다. 1. 치석다침착자의 치태 건조중량당 10%의 탄수화물, 43%의 단백질, 18%의 지질, 4.3%의 Ca, 0.15%의 Mg, 0.29%의 K과 2.87%의 P으로 구성되어 있다. 2. 탄수화물, 지질, K은 비세포 성분획에서 단백질, Mg, 세포성분획에서 많이 차지하였다. 3. Ca, Mg, P의 함량은 치석다침착자의 치태에서 K의 함량은 치석경침착자의 치태에서 높았다. 4. Ca/P비는 비세포성분획보다 세포성 분획에서 훨씬 낮았다.

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The Structure of Assure Information Security In Mobile Environment Based Mobile PKI (모바일 PKI 기반의 이동 보안 구조)

  • 서세영;신승수;최승권;조용환
    • Proceedings of the Korea Contents Association Conference
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    • 2003.11a
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    • pp.132-139
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    • 2003
  • In this paper, we design and implement the public key certification the structure of assure information security which play an important role in PKI (Public Key Infrastructure). The structure of assure information security consists of Root-CA, Home-network and Foreign-network. CA will going to gave the mandate to Home-Agent or Foreign-Agent when they request. The structure of assure information security is various characteristic : more then high speed, mobile network, and low cost more then previous structure of assure information security.

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The Authentication Structure Based Mobile PKI (모바일 PKI 기반한 인증 구조)

  • 김미혜;서세영
    • The Journal of the Korea Contents Association
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    • v.4 no.1
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    • pp.67-75
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    • 2004
  • In this paper, we design an authentication model based mobile PKI (Public Key Infrastructure). The authentication mood consists of Root-CA Home-network agent and Foreign-network agent. CA will going to gave the delegation ticket to Home-Agent or Foreign-Agent when they request. The authentication mode information security is various characteristic more then high speed, mobile network and low cost more then previous structure of assure information security.

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치석의 무기질 분석에 관한 연구

  • Kim, Hyun-Pung
    • The Journal of the Korean dental association
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    • v.12 no.7
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    • pp.521-526
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    • 1974
  • This observation was carried out to investigate the inorganic constituents of dental calculus of varying locations and different ages. Supragingival calculus was obtained from 540 patients with the periodontal disease. The results were as follows : 1. The weight loss rate in ashing by aging was the highest in the 2nd decade (63.7%) and followed in order by 3rd decade (60.8%), seventh decade 959.8%), fourth decade (55.9%), sixth decade (52.6%) and fifth decade (43.2%). 2. The weight loss rate by ashing was more prominent in the buccal surfaces of the upper posterior teeth (62.0%) than in the lingual surface of the lower anterior teeth (59.7%). 3. The difference in contents of the inorganic constituents by sex was not remarkable. (male, Ca:373.0 P:333.9, female, Ca: 380.2, P: 339.6 ㎍/mg dry weight) 4. In the dry calulus, contents of the inorganic constituents were as follows: Ca:325.8 P:269.10 Mg:1.21 Na:8.44 K:1.32, Zn:0.67 ㎍/mg. 5. The Ca/P ratio was the lowest in the upper anterior region (1.11) and the highest in the lower posterior region (1.29) and the average was 1.20.

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