• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.10a
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• pp.165.2-166
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• 2003

최소 가공 수박의 가공과 유통 중 품질 열화에 있어 가장 중요한 문제는 과육의 조직연화이다. 따라서 본 연구에서는 세포 분리를 제한하고 과실 조직의 경도를 유지시키는데 효과가 있는 것으로 알려져 있는 칼슘제제 중 calcium chloride, calcium lactate, CaO를 처리하여, 최소 가공 수박에서 조직연화로 발생되는 품질저하 억제에 가장 효과적인 칼슘제제를 알아보고자 하였다. 수박의 과육부위를 2cm의 정육면체 형태로 절단한 후, 0.5% calcium chloride, 0.5% calcium lactate, 0.05% CaO용액에 3분 동안 침지시켰다. 그리고 fan을 이용하여 상온에서 30분 동안 draining한 다음 PP tray에 담아 PP film로 sealing하고 $10^{\circ}C$에서 일주일간 저장하며 품질변화를 살펴보았다. 호흡특성은 0.05% CaO 및 0.5% calcium chloride용액을 처리한 구가 호흡률이 가장 낮게 나타났다. 가용성고형분은 처리 직후 처리구의 함량이 무처리구에 비해 다소 낮아지는 경향을 보였으나, 저장기간 중 0.5% calcium chloride 용액처리구는 다른 처리구에 비하여 큰 함량 변화 없이 당도가 일정 수준으로 유지되었다. pH 변화는 0.5% calcium chloride용액 처리구에서 변화가 가장 작았으며, 경도변화 역시 저장 초기값과 비교하였을 때보다 감소가 적게 일어나 다른 처리구에 비하여 연화 저해에 효과가 있는 것으로 확인되었다. 따라서 최소가공 수박에 대해 칼슘제제 중 calcium chloride용액 처리가 품질유지에 가장 효과적임을 알 수 있었다.

• Journal of Animal Science and Technology
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• v.48 no.5
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• pp.671-682
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• 2006

This experiment was conducted to investigate the effects of dietary Ca levels and some feed additives such as isoflavone and casein phosphopeptide (CPP) on eggshell quality and hatching egg production in aged egg-type breeder hens. A total of three hundred and sixty, 56-week-old Hy-Line Brown breeder hens were divided into six groups and fed experimental diets of two levels of Ca (3.3% or 3.6%) either with addition of 0.2% isoflavone, 0.5% CPP or devoid of all for 5 weeks. There were no significant differences in laying performances and settable egg production among the groups. Significant increases (P<0.05) in eggshell strength were observed with increasing dietary Ca and addition of isoflavone, but not with addition of CPP. Fertility and hatchability were not influenced by dietary Ca and addition of isoflavone or CPP. The treatment had few significant effects on tibial proximal compositions and breaking strength. The concentrations of Ca, P, estrogen and calcitonin in serum were not affected by the dietary treatments. These results indicated that relatively high level of dietary Ca in combination with isoflavone had a beneficial effect on improving eggshell quality in aged egg-type breeder hens. But hatching egg production was not affected by dietary isoflavone or CPP.

• Journal of Bio-Environment Control
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• v.8 no.2
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• pp.99-107
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• 1999

The experiment was conducted to investigate the relationship between $Ca^{2＋}$ uptake and development of fermented fruit through the forced-air circulation of ambient fruit. Air circulation of ambient fruits were adjusted with 0.3m/sec wind velocity for three hours a day from 10：00 to 13：00. Treatments consisted of 0, 10 day, 20 day, 30 day of forced air circulation of ambient fruit. Although the results varied depend on the duration forced air circulation, in general, treated fruit increased fruit weight, flesh thickness, fruit hardness, soluble solids, and chromaticity, and decreased the number of fermented-fruit. $Ca^{2＋}$ content in fruit.

• Journal of Periodontal and Implant Science
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• v.25 no.1
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• pp.99-110
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• 1995

치주치료의 궁극적 목표인 치주조직 재생에 대한 관심이 높아지는 가운데 치주조직 재생에 주된 역할을 하는 치주인대세포와 치근면 탈회에 의한 신부착 효과에 관한 많은 연구가 시도되고 있다. 그러나 광범위한 연구에도 불구하고 어떤 치근면 처리가 신부착 형성에 가장 효과적인지는 아직 논란의 대상으로 남아있다. 이에 저자는 결합조직 부착에 의한 재생에 필수적인 치은 및 치주인대 섬유아세포의 부착 및 증식을 tetracycline-HCL(TC)과 citric acid(CA)로 각각 처리된 상아질 표면에서 비교관찰하여 치주조직 재생에의 기여도를 추정하기 위해 본 연구를 시행하였다. 교정치료를 위하여 본원에 내원한 환자의 제일 소구치의 건강한 치은조직을 채취하고, 발치 후 효소처리에 의한 치주인대세포를 얻은 후 각각 세포배양하였다. 상아질 절편은 $3{\times}3{\times}0.2mm^3$로 준비하고 TC과 CA처리군으로 나눈 후 각각 치은 및 치주인대 섬유아세포를 부착시키고 초기부착은 8시간까지, 증식은 7일까지 고나찰하여 다음의 결과를 얻었다. 1. 각 군의 세포 부착은 시간이 지남에 따라 증가되었다. 2. 모든 군에서 7일째 빠른 증식상을 보였다. 3. 초기 부착시 치은과 치주인대 섬유아세포의 반응에는 유의한 차이가 없었다. 4. 반면에 치은과 치주인대 섬유아세포는 증식속도에서 차이를 나타내었으며 치은 섬유아세포가 좀 더 빠른 성장을 보였다. 5. 치근면 탈회에 따라 초기부착과 증식상에서 모두 유의성 있는 증가를 보였다. 6. CA는 초기부착에서, TC는 증식장에서 더욱 효과적으로 작용했다.

• Food Science and Preservation
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• v.16 no.1
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• pp.128-133
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• 2009

We performed an experiment of keeping the extension of raw and semi-dried flounder (Pleuronectes herzensteini). The effect of with (WG) or without gill (OG), drying degree (20% drying: 20D, 40% drying: 40D) and storage temperature($5^{\circ}C$) and 0.1% chitosan-ascorbate (CA) treatment of vacuum packaging flounder on the growth of contaminated microorganism during storage for 10 days were investigated. Total aerobacter (TA) in the OG-treated raw flounder was $0.29{\sim}0.44$ log cycle lower than that of WG-treated flounder. Also, the number of Staphylococcus aureus (SA) and E. coli (EO) in OG were lower compared with WG. The number of TA, SA and EO in 20 D among 0 D, 20 D and 40 D stored at $5^{\circ}C$ were lowest. Especially, the SA and EO was $0.13{\sim}0.53$, 0.3-0.88, and 0.13-0.74 log cycle lower compared with raw flounder. The growth of TA, SA and EO separated from raw flounder in tryptic soy broth were completely inhibited by 0.1% CA. The anti-biotical effect of CA of two microorganisms SA and EO that separated from flounder, and the growth of all of them were 90% (SA), 96% (EO) inhibited at the 0.1% CA. The inhibition times at $37^{\circ}C$ in soy broth was 36 hr. However when CA was added directly to flounder, it appeared inhibition effect to 0.88 log cycle. The effect of CA was better when gills removed and 20% drying.

• Journal of Bio-Environment Control
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• v.14 no.2
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• pp.128-136
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• 2005

The objective of this study was to determine the effect of calcium concentrations in fertilizer solution on growth and cut flower quality of chrysanthemum 'Biarritz' To achieve this, deficiency symptom and growth characteristics as influenced by controlled Ca concentrations in fertilizer solution were investigated. The analysis of plant tissue and soil solution of root media were also conducted to secure optimum concentration for plant growth. Calcium deficiency developed on the very youngest leaves and the young leaves developed 'cupped' shape as they expand. The cut flower length of 0, 3.0 and 6.0 mM treatments were 105.8, 106.5, and 107.3cm, respectively. Elevated Ca concentration within the range from 0 to 6.0mM in fertilizer solution increased cut flower weight. The cut flower weight of control and 6.0mM treatment were 51.6 and 59.4 g, respectively. The tissue Ca content of 6.0mM treatment in which crops showed the highest growth among treatments was $3.09\%$ based on the dry weight of the youngest fully expanded leaves. From the results, it seems necessary to maintaining tissue Ca content higher than $2.8\%$. Soil Ca concentrations increased as K concentrations in fertilizer solution were elevated. Ca concentration in 0.0, 1.5, 3.0, 4.5 and 6.0mM treatments were 5.0, 7.4, 12.1, 16.5 and $28.2mg{\cdot}L^{-1}$, respectively, at harvesting stage. It is suggested that Ca concentration higher than $25.4mg{\cdot}L^{-1}$ in soil solution of root media is required to maintain normal growth.

• Journal of Life Science
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• v.24 no.2
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• pp.112-117
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• 2014

We investigated the effects of extracts from Rubus coreanus (RC) and Artemisia princeps var. orientalis (AP) on DNA damage response in ultraviolet B (UVB)-exposed HaCaT cells. Cell activity upon treatment for 24 h with RC or AP alone was similar to or greater than that of the nontreated control. When UVB-exposed cells were postincubated for 24 h in medium containing RC or AP, cell activity increased in a concentration-dependent manner. Nuclear fragmentation analysis showed that postincubation with RC or AP decreased UVB-induced apoptosis by about 20% and 15%, respectively, of that in cells postincubated with growth medium. When UVB-exposed cells were postincubated for 24 h in medium containing RC or AP, the level of cyclobutane pyrimidine dimer decreased in a concentration- dependent manner. Western blot analysis showed that treatment of cells not exposed to UVB with RC or AP alone did not significantly change the levels of phospho-p53 and GADD45 protein. Interestingly, when UVB-exposed cells were postincubated for 24 h in medium containing RC or AP, phospho-p53 and GADD45 levels decreased in a concentration dependent manner. Our results suggest that RC and AP extract assist the survival of UVB-exposed cells in parallel with a decrease in levels of UVB-induced DNA damage and damage-response proteins, such as p53 and GADD45.

• Journal of Animal Science and Technology
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• v.50 no.3
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• pp.407-416
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• 2008

본 연구는 CaCl2 용액의 주사량이 품종별 암사슴의 뿔 발생 유도에 미치는 영향을 구명하기 위하여 꽃사슴, 레드디어 및 엘크 암컷 각 6두에 대해 30% CaCl2 용액의 주사량을 1ml?, 1.5ml? 및 2ml?의 3처리구로 나누어 전두골 좌, 우 양쪽 골막에 3월과 7월 두 차례 주사한 후 사슴뿔 발생 유도율, 사슴뿔 생산량, 발육성적을 조사하였다. 품종별 암사슴의 뿔 발생은 꽃사슴이 1ml? 처리구의 한 마리 한쪽에서만 발생하였고, 레드디어는 2ml? 처리구의 한 마리 한쪽에서만 유도되어 꽃사슴과 레드디어의 뿔 발생 유도율은 8.3%였다. 엘크 암사슴은 1ml? 처리구에서는 뿔 발생이 일어나지 않았으며, 1.5ml?와 2ml? 처리구에서는 모두 뿔 발생이 유도되어 유도율은 67%였다. 뿔 생산량은 꽃사슴의 경우 길이 21cm?이고 무게 70g 이었으며, 레드디어는 길이 18.5cm?에 무게 83g의 뿔을 생산하였다. 엘크는 3월 1.5ml? 처리구의 길이가 평균 34±3cm?이었고 무게는 526~1,008g이며, 2ml? 처리구는 길이가 평균 39±8cm?, 무게는 693~1,379g의 범위에 있었다. 엘크의 2ml? 처리구는 7월에 주사한 것이 뿔 길이와 뿔 생산량에서 3월의 것보다 적었다. 암사슴 뿔의 발육은 엘크의 경우 주사 후 20일경에 길이 0.5~1cm? 정도의 돌기로 자랐고, 40일까지는 유발된 상처와 함께 1~3cm?정도의 완만한 성장을 보였다. 본격적인 성장은 처리 후 60일경이 지나야 일어났고, 120일경에 절각을 하였을 때 뿔 길이는 28~44cm?의 범위에 있었다. 생산된 암사슴 뿔을 세로로 절단하였을 때 전체 길이의 위쪽 70%는 골화가 되지 않은 상태였다. 암사슴의 뿔 발생 유도는 번식성적에는 전혀 영향을 미치지 않았다. 봄에 처리한 사슴의 재생 뿔 발생은 불규칙하게 일어났고, 재생 뿔 발생률은 평균 45%였으며, 주기가 진행될수록 길이와 생산량은 줄어드는 경향을 보였다. 결론적으로 30% CaCl2 용액의 주사량을 조절하여 처리하면 꽃사슴, 레드디어 및 엘크의 암컷에서 뿔 발생을 유도할 수가 있으며, 엘크 암사슴의 뿔 발생 유도를 위한 최적 주사량은 1.5~2ml? 범위였다.

• Journal of Sericultural and Entomological Science
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• v.45 no.1
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• pp.34-45
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• 2003

Silk was treated with calcium hydroxide for degumming at different treatment times, temperatures and Ca(OH)$_2$ concentration to optimize degumming conditions in this thesis. After degumming, soluble and insoluble sericin were seperated and then the soluble sericin was characterized by measuring the average degree of polymerization (D.P.), lysinoalanine (LAL) content, DSC, and by amino acid analysis. And degummed silk fibroin was characterized by measuring tenacity and SEM. Degumming loss was increased by increasing the treatment time and temperature until about 30 minutes. After then, a slight difference was found along with treatment times at the Ca(OH)$_2$ concentrations of 0.07% and 0.1% solutions. After degumming, insoluble sericin ratio on degumming solution was increased by increasing treatment temperature at Ca(OH)$_2$ 0.04% solution. At the concentration Ca(OH)$_2$ of 0.07%, a soluble ratio was almost 100% regardless of treatment time and temperature. At the beginning of treatment, insoluble ratio was high at Ca(OH)$_2$ 0.1% solution but it was decreased by increasing treatment time. At the Ca(OH)$_2$ concentration of 0.04%, D.P. of soluble sericin was maintained as a constant value of 10 at 100$^{\circ}C$ although treatment time was increased. However, at 80$^{\circ}C$ and 90$^{\circ}C$, it was hard to prepare a soluble sericin having a constant D.P. by increasing treatment time. At the Ca(OH)$_2$ concentration of 0.07%, D.P. was almost 10 irrespective of treatment temperature and time. Soluble sericins with high D.P. of 20∼30 were obtained at 0.1% and 100$^{\circ}C$. LAL was not detected in soluble sericin. As the results of amino acid analysis, it showed that Ca(OH)$_2$ degumming reduced the contents of hydroxy amino acids like Ser., Thr. and Tyr. In DSC analysis of soluble sericin, endothermic peak by thermal deformation and pyrolysis showed at 189$^{\circ}C$ and at 299$^{\circ}C$, respectively. The tenacities of degummed silk were 15∼30% lower than that of raw silk. And it was decreased with increasing treatment time. From the morphological study, the thickness of degummed silk fibroin became thinner by increasing degumming loss. The roughness of a silk fibroin surface was appeared as treatment concentration was increased.

• Horticultural Science & Technology
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• v.33 no.3
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• pp.375-382
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• 2015

A controlled atmosphere (CA) regimen was optimized during 3 consecutive harvest seasons as the basis of practical modified atmosphere packaging (MAP) storage for quality maintenance and extension of storage potential of fresh Ligularia fischeri leaves. Leaves were harvested in April or May and forced-air cooled to $4^{\circ}C$ before punch-hole MAP (control, where gas concentrations were same as air) and CA treatments. CA regimens adjusted stepwise during 3 experimental years were: 1 and 3% $O_2$, respectively combined with 5 and 10% $CO_2$ in the first year, 3% $O_2$ fixed in combination with 0, 2.5, and 5% $CO_2$ in the second year, and 3% $O_2$ fixed in combination with 2.5 and 5% $CO_2$ in the third year. In the first year, higher incidence of black discoloration was observed with the reduction of respiration under 10% $CO_2$ CA conditions regardless of $O_2$ levels at 1 or 3%. In the second and third year, the incidence of the disorder seemed not to be clearly relevant to CA conditions showing slightly higher incidence only after 4- or 5-week storage + 5-day shelf life. Although texture and appearance quality were maintained better under the 3% $O_2$ + 2.5% $CO_2$condition after 4-week storage + 5-day shelf life, effects of CA on the extension of storage period was slight. Overall results indicated that Ligularia fischeri leaves are very susceptible to $CO_2$ injury. $CO_2$ concentration should be adjusted below 2.5% for safe and effective CA or MAP storage to maintain quality even during short-term storage.