• Journal of Embryo Transfer
• /
• v.18 no.2
• /
• pp.85-90
• /
• 2003

This study examined the viability of canine oocytes following storage at 4 or 38$^{\circ}C$ for 5 h. Cumulus intact oocytes were collected from domestic dog following ovariohysterectomy at local veterinary clinics. In Exp I, the oocytes that collected from ovary transport different temperatures (4 or 38$^{\circ}C$) for 5 h, were cultured for (24 or 48 h). Survivability of oocytes judged by morphological appearance and PI (propidium iodide) staining. The survival rates at 4$^{\circ}C$ ovary transport group showed significantly lower than control group (0%; 0/129 vs. 72.9%; 129/177) 48 h after culture (P<0.05). In Exp II, to assess nuclear maturation of control group oocytes (ovary transported at 38$^{\circ}C$) after in vitro cultured for 24, 48 or 96 h. After 24 h and 48 h of culture, the metaphase I to metaphase II stages (MI to MII) was 8.3% (6/72) and 8.9% (9/101), and which was not increased at 96h (9.5%; 8/84). These results show that canine oocytes remarkably sensitive to low temperature and the percentage of oocytes reaching MI to MII did not increase 96 h after culture.

• Korean Journal of Food Science and Technology
• /
• v.10 no.4
• /
• pp.409-414
• /
• 1978

The total amount of lipid content in the mullet roe is 20.5%, and iodine value and unsaponifiable matters content are 118 and 38.7%, respectively. The lipid composition of the muscle, roe and liver of the mullet, Mugil cephalus, shows differences. Triglyceride, wax esters, and free fatty acids are mainly contained in the lipids of the muscle, roe and liver, respectively. The mullet roe lipids are mainly composed of 59.1% of wax esters with a trace of sterol esters, 26.9% of polar lipids with pigments, 9.0% of triglyceride plus a trace of free fatty alcohols and fatty acids, and 3.0% of sterol contaminated with a trace of fatty alcohols. The major fatty acids of wax esters are C16 : 0, 47.5%, C18 : 1, 23.0%, C16 : 3, 6.5%, C20 : 5, 4.0%, those of triglyceride are C16 : 1, 25.1%, C18: 1, 16.7%, C16 : 0, 16.3%, C22 : 1, 7.9%, C18 : 0, 5.5%, C22 : 6, 4.4%, and those of polar lipids are C16 : 0, 35.0%, C18 : 1, 24.7%, C16 : 1, 6.1%, C20 : 5, 5.3%, C22 : 6, 4.2%. The major alcohols of wax esters are 51.0% of cetyl alcohol, 18.2% of palmitoleyl alcohol, and 10.7% of oleyl alcohol, and considerable amounts of odd-numbered alcohols such as C15 : 0, C15 : 1, C17 : 0, C17 : 1 and C19 : 1 are also found.

• Korean Journal of Metals and Materials
• /
• v.50 no.1
• /
• pp.1-7
• /
• 2012

The fracture resistance and tensile properties of $Y_2O_3$ oxide dispersion strengthened steel containing 9 wt% Cr(9Cr-ODS) were measured at various temperatures up to $700^{\circ}C$. The fracture characteristics were compared with those of commercial E911 ferritic/martensitic steel. The strength of 9Cr-ODS was at least 30% higher than that of E911 steel at the test temperatures below $500^{\circ}C$. The strength difference between the two materials was almost diminished at $700^{\circ}C$. 9Cr-ODS showed cleavage fracture behavior at room temperature and unstable crack growth behaviors at $300^{\circ}C$ and $500^{\circ}C$. The J-R fracture resistance of 9Cr-ODS was much lower than that of E911 steel at all temperatures. It was deduced that the coarse $Cr_2O_3$ particles that were formed during the alloying process provided the crack initiation sites of cleavage fracture in 9Cr-ODS.

• Journal of Aquaculture
• /
• v.17 no.2
• /
• pp.103-108
• /
• 2004

Effects of temperature and salinity were investigated on physiological responses of Mytilus coruscus seedlings. Temperature tolerance and survival of M. coruscus, were examined at temperature 20, 25, 28, 30 and 35$^{\circ}C$ for 9 das. Survival of M. coruscus was 90% at temperature 2$0^{\circ}C$ and $25^{\circ}C$. LT$_{50}$ (lethal temperature) of 9 days was at 27.1$^{\circ}C$. The respiration and filtration rates of M. coruscus were increased with temperature up to $25^{\circ}C$, and decreased with temperature ranged from $25^{\circ}C$ to 3$0^{\circ}C$. LS$_{50}$ (lethal salinity, psu) of 9 days at 1$0^{\circ}C$, 15$^{\circ}C$ and $25^{\circ}C$ were 17.01 psu, 19.95 psu and 21.79 psu respectively. Salinity affected survival of M. coruscus with higher temperature. However the respiration and filtration rates were reduced with lower salinity.

• Molecules and Cells
• /
• v.25 no.2
• /
• pp.184-195
• /
• 2008

We examined the role of c-FLIP in the motility of HeLa cells. A small interfering RNA (siRNA) directed against c-FLIP inhibited the adhesion and motility of the cells without affecting their growth rate. The long form of c-FLIP ($c-FLIP_L$), but not the short form ($c-FLIP_S$), enhanced adhesion and motility. Downregulation of $c-FLIP_L$ with siRNA decreased phosphorylation of FAK and ERK, while overexpression of $c-FLIP_L$ increased their phosphorylation. Overexpression of FAK activated ERK, and enhanced the motility of HeLa cells. FRNK, an inhibitory fragment of FAK, inhibited ERK and decreased motility. Inhibition of ERK also significantly suppressed $c-FLIP_L$-promoted motility. Inhibition of ROCK by Y27632 suppressed the $c-FLIP_L$-promoted motility by reducing phosphorylation of FAK and ERK. Overexpression of $c-FLIP_L$ increased the expression and secretion of MMP-9, and inhibition of MMP-9 by Ilomastat reduced $c-FLIP_L$- promoted cell motility. A caspase-like domain (amino acids 222-376) was found to be necessary for the $c-FLIP_L$-promoted cell motility. We conclude that $c-FLIP_L$ promotes the motility of HeLa cells by activating FAK and ERK, and increasing MMP-9 expression.

• BMB Reports
• /
• v.40 no.3
• /
• pp.448-452
• /
• 2007

We examined the contribution of CYP2C9 and CYP2C19 genotypes and drug interactions to the phenytoin metabolism among 97 Korean epileptic patients to determine if pharmacogenetic testing could be utilized in routine clinical practice. The CYP2C9 polymorphism is a wellknown major genetic factor responsible for phenytoin metabolism. The CYP219 polymorphism, with a high incidence of variant alleles, has a minor influence on phenytoin treated Koran patients. Using a multiple regression model for evaluation of the CYP2C9 and CYP2C19 genotypes, together with other non-genetic variables, we explained 39.6% of the variance in serum phenytoin levels. Incorporation of genotyping for CYP2C9 and CYP2C19 into a clinical practice may be of some help in the determination of phenytoin dosage. However, because concurrent drug treatment is common in patients taking phenytoin and many environmental factors are likely to play a role in drug metabolism, these factors may overwhelm the relevance of CYP polymorphisms in the clinical setting. Further investigations with an approach to dose assessment that includes comprehensive interpretation of both pharmacogenetic and pharmacokinetic data along with understanding of the mechanism of drug interactions in dosage adjustment is warranted.

• The Journal of Korean Society of Virology
• /
• v.27 no.2
• /
• pp.129-135
• /
• 1997

Serum samples from 123 males and 123 females collected by age in 1996 were analyzed for antibodies against surface antigen of Hepatitis B virus and C22-3, C200 antigens of Hepatitis C virus. Sera from the children under the age of 10 showed 30% seropositivity to the surface antigen of Hepatitis B virus, 33.3% in $10{\sim}19$ year group, 20% in $20{\sim}29$ year group, 17.6% in $30{\sim}39$ year group, 3.3% in $40{\sim}49$ year group, 5.9% in $50{\sim}59$ year group, 8,3% in $60{\sim}69$ year group, 2.9% in $70{\sim}79$ year group, but antibody could not found in $80{\sim}86$ year group. 12 out of 123 male sera were positive, 19 out of 123 female sera were positive and overall rate of positivity of antibody against surface antigen of Hepatitis B virus was 12.6%. Serum samples from peoples under the age of 30 had not antibody against C22-3, C200 antigens of Hepatitis C virus. The positivity rate was 2.9% in $30{\sim}39$ year group. 5 out of 30 sera from $40{\sim}49$ year age group were positive, and 3 positive sera showed extremely high titer (1:524,288) but the titers of two remaining sera were 1:32, 1:8,192 respectively. 5.9% was positive in $50{\sim}59$ year group, 8.3% in $60{\sim}69$ year group, 11.8% in $70{\sim}79$ year group but all negative in $80{\sim}86$ year group 6 out of 123 male sera were positive (4.9%), 9 out of 123 female sera were positive (7.3%). Overall rate of positivity of antibody against C22-3, C200 antigen of Hepatitis C virus was 6.1 %. None out of 246 sera had both antibodies against Hepatitis B virus and Hepatitis C virus.

• Applied Biological Chemistry
• /
• v.13 no.3
• /
• pp.193-195
• /
• 1970

The cultural conditions of the osmophilic yeasts for higher concentration of NaCl selected in the previous report were examined and the results obtained were as follows. 1) The strain $T_3\;and\;T_8$ were grown exceedingly well on the media containing 15 percent of NaCl and $T_5\;and\;T_9,\;T_{10}\;and\;T_{11}$ on the media containing 5 percent of NaCl. 2) The optimum temperature for growth of the strain $T_3\;and\;T_5$ was $30^{\circ}C,\;T_8\;T_{10}$ and $T_{11}\;was\;25^{\circ}C\;and\;T_9\;was\;35^{\circ}C.$ 3) Their lethal temperature was $60^{\circ}C$ (treatment for 10 minutes). 4) The optimum pH for growth of the strain $T_3\;and\;T_8$ was pH 4.0, $T_5$ was pH 6.0 and $T_9\;T_9\;and\;T_{11}$ was pH 5.0, respectively.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.20 no.9
• /
• pp.405-411
• /
• 2019

In this study, tensile tests were carried out on materials (A516-60, A283-C, A285-C, and SB410) for structural and pressure vessels at temperatures of $20^{\circ}C$, $-20^{\circ}C$, and $-40^{\circ}C$, and the changes in the mechanical properties were analyzed. Compared to the results at $20^{\circ}C$, the average yield stress increased by 6.4% and 7.5% at $-20^{\circ}C$ and $-40^{\circ}C$ for A516-60, while the average tensile stress increased by 1.3% and 4.1%, respectively. The average elongation decreased by 4.7% and 20.4% at these temperatures. In the case of A283-C, the average yield stress increased 8.8% and 9.8%, the average tensile stress increased by 4.1% and 5.9%, and the average elongation rate decreased by 7.4% and 9.9% at $-20^{\circ}C$ and $-40^{\circ}C$, respectively. For A285-C, the average yield stress increased by 1.8% and 8.6%, and the average tensile stress increased by 2.6% and 5.3%, respectively, but there was little change in the average elongation. Finally, for SB410, the average yield stress increased by 7.1% and 11.8%, the average tensile stress increased by 4.3% and 5.5%, but the average elongation rate decreased by 8.7% and 13.5%, respectively.

• Journal of Mushroom
• /
• v.13 no.3
• /
• pp.256-261
• /
• 2015

This study was conducted to reduce contamination ratio of oyster mushroom bottle cultivation. The optimal conditions of substrate sterilization for reducing of contamination ratio were at $121^{\circ}C$ for 90 min. In addition, UV-C irradiation is good for lower contamination ratio to continue over 6 hours at cooling and inoculation room after sterilization. The contamination ratio and density of microorganisms of substrate were showed 0% after sterilization at $121^{\circ}C$ for 90 min. Trichoderma sp., main pathogen of mushrooms, was detected from substrate after sterilized during 2 or 4 hours at $101^{\circ}C$ and $105^{\circ}C$, respectively. The amount of electricity used was the lowest at $121^{\circ}C$ for 90 min than that of other sterilization conditions. The UV-C irradiation treatment was used UV-C lamp(40 watts) in the inoculation room($56m^3$). The density of bacteria did not detected after UV-C irradiation for 6 hours. And the death ratio of bacteria and Trichoderma sp. was 99.9% after UV-C irradiation for 6 hours. However, in the same UV-C irradiation time, the death ration of Cladosporium sp. was 90.9%. Therefore, the death ratio of fungi was lower than that of bacteria at the same UV-C irradiation treatment.