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Germination Rate and Microbial Safety during Cultivation of Disinfected Seeds (새싹 종자 소독 여부에 따른 발아율과 재배기간별 미생물 오염도)

  • Park, Eun-Jung;Kwon, Joong-Ho;Lee, Yeon-Kyung
    • Food Science and Preservation
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    • v.16 no.2
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    • pp.292-298
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    • 2009
  • This study investigated the microbial safeties and germination rates of five domestic sprout species(alfalfa, broccoli, clover, red cabbage, and red radish) grown from disinfected seeds. The 48 h germination rates of all seeds were over 90%, regardless of treatment. Seed total plate count(TPC) and coliform levels were reduced significantly(p<0.05) by treatment with 20,000 ppm calcium hypochlorite solution at $25^{\circ}C$ for 15 min, following FDA recommendations. However, after germination, all sprouts regardless of treatment exhibited bacterial counts of $10^7-10^8CFU/g$. Listeria monocytogenes was detected at $10^3-10^4CFU/g$ on germinated non-disinfected clover seeds at days 1, 2, and 5. In conclusion, although sprout germination from disinfected seeds potentially permits the growth of sprouts with lower pathogen counts, there were no significant differences in TPC or coliform levels between sprouts grown from disinfected seeds and control sprouts. Further work is needed to improve the microbial safety of cultivated sprouts and to find optimal conditions for seed germination.

Nano-structure and Magnetic Properties of FePd Superlattice Thin Film (FePd 인공격자박막의 나노구조 및 자기적 특성)

  • Kang, J.G.;Chung, I.S.;Koo, J.W.;Koh, J.H.;Koo, S.M.;Nam, S.M.;Ha, J.G.
    • Journal of the Korean Magnetics Society
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    • v.18 no.5
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    • pp.190-194
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    • 2008
  • Epitaxial $L1_0$ FePd (001) thin films were successfully manufactured by sputtering deposition method. The structure and magnetic properties of FePd thin films were characterized as a function of Fe compositions. It was found that the long-range ordering parameter had a maximum for the stoichiometric composition, whereas the magnetic anisotropy had a maximum as the Fe content is decreased to slightly above the stoichiometric composition. This indicates that the stoichiometry is directly contributed to the chemical ordering and the magnetic anisotropy. These results imply that nonstoichiometric FePd compositions, with a slight excess of Pd, may in fact be preferred for applications that require high magnetic anisotropy.

Study on the Linear Magnetic Field Sensor Using ΔE in Amorphous Ribbon (비정질 리본의 ΔE 특성을 이용한 선형 자기센서에 관한 연구)

  • Park, S.Y.;Kim, C.G.;Ryu, K.S.;Kim, D.Y.
    • Journal of the Korean Magnetics Society
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    • v.23 no.6
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    • pp.205-208
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    • 2013
  • The width dependence of Delta E has been studied by impedance resonance method in Fe-rich amorphous ribbon of as received state. It can be explained by single domain model include effective magnetic field $H_{eff}$ and demagnetization factor $N_d$. Wider width ribbon's effective is smaller than narrow it. Young's modulus also smaller than narrow width. These result well agreement in calculation. It has been also studied that linear magnetic field sensor using width dependence of Delta E under the field range 0 Oe~80 Oe. The sensitivity of the sensor was found to be 95 Hz/Oe.

Effects of supplementation of hairy vetch on the quality of whole crop barley silage (헤어리베치의 첨가가 맥류 사일리지의 품질에 미치는 영향)

  • Jang, Won-Sup;Yang, Byung-Mo;Heo, Jung-Min;Lee, Hyung-Suk;Lee, Soo-Kee
    • Korean Journal of Agricultural Science
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    • v.42 no.4
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    • pp.383-388
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    • 2015
  • This study was conducted to investigate the effects of hairy vetch supplementation on quality of winter crop silage. There were 4 treatments (addition levels of hairy vetch ; 0, 5, 15, and 30%) with 3 replicates. Experimental silages stored for 40 days at room temperature ($20-25^{\circ}C$). THe silage crude protein level was improved (P<0.05) while hairy vetch supplementation increased. However, no difference was found (P>0.05) in crude fat, NDF and ADF of the silage while hairy vetch supplementation increased. The silage pH was increased (P<0.05) but lactic acid level was decreased (P<0.05) while hairy vetch supplementation increased. Nonetheless, acetic and butyric acids concentrations were increased (P<0.05) while hairy vetch supplementation increased. Sucrose, glucose and fructose levels were increased (P<0.05) while hairy vetch supplementation increased. Although negative effects were detected in whole crop barley silage while hairy vetch supplementation increased, optimum level of hairy vetch supplementation could be overwhelmed its negative effects on whole crop barley silage. Thus, the results of present study suggested that 15% hairy vetch supplementation of whole crop barley silage would be beneficial its quality maintenance compared to whole crop barley silage per se.

The Study on Payment Difference of Health Examination Users in University Hospital Medical Center (대학병원 건강검진센터 이용자의 검진금액 차이에 관한 연구)

  • Cho, Duk-Young
    • Management & Information Systems Review
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    • v.32 no.2
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    • pp.47-58
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    • 2013
  • This study analyzes on payment difference of users in sex, age, region using 23-months data by a university medical center to figure out factors which help to hospital management. The meaningful results of this study as follow. First of all, there is not statistically significant difference between men and women. Secondly, the researcher categorizes users under 40s into group A and 40s users into group B, those in 60s or older into group C. Then, researcher accepted H2 that there might be a difference on the age range of users in each group. Finally, In the results on difference analysis of the payment, there is only statistical difference between Busan and Ulsan Gyeongnam. These results is expected to provide basic information for efficient management of medical center.

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The Study on DME (dimethyl ether) Conversion Over the Supported SAPO-34 Catalyst (담지된 SAPO-34 촉매상에서 DME(dimethyl ether) 전환 연구)

  • Lee, Su-Gyung;Yoo, Byoung-Kwan;Je, Han-Sol;Ryu, Tae-Gong;Park, Chu-Sik;Kim, Young-Ho
    • Journal of Hydrogen and New Energy
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    • v.22 no.2
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    • pp.232-239
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    • 2011
  • DME has received much attention because of its possible use as a fuel and a chemical feedstock. Chemical conversion of DME to olefin (DTO) over various SAPO-34 catalysts was carried out using a fixed bed reactor. Main products of the reaction were light olefins such as ethylene, propylene and butenes. The best reaction conditions for high life time of the catalyst and high selectivity of light olefins were a reaction temperature of $400^{\circ}C$ and a WHSV of $3.54h^{-1}$. In addition, it was found that the deactivation of a SAPO-34 catalyst can be significantly suppressed by the addition of $ZrO_2$ as a supporter.

Changes of Index Microorganisms and Lactic Acid Bacteria of Korean Fermented Vegetables (Kimchi) during the Ripening and Fermentation-Part 1 (김치의 숙성 및 발효중 오염지표미생물과 유산균의 변화-제1보)

  • Kim, Jong-Gyu;Yoon, Joon-Sik
    • Journal of Environmental Health Sciences
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    • v.31 no.1
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    • pp.79-85
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    • 2005
  • This study was undertaken to investigate the changes of index microorganisms and lactic acid bacteria of traditional Korean fermented vegetables (kimchi) during the ripening and fermentation period. A type of kimchi, baechoo-kimchi, was prepared and stored at $10^{\circ}C$ for 8 days. The numbers of the total aerobic bacteria, psychrotrophilic bacteria, coliform bacteria, and Escherichia coli in the kimchi and also in raw materials of the kimchi (Chinese cabbage, green onion, ginger, garlic, and red pepper) were counted using appropriate media. The highest number of aerobic bacteria was detected from ginger, then red pepper, then garlic, then Chinese cabbage, and lowest number from green onion. The highest number of psychrotrophilic bacteria was detected from red pepper, then Chinese cabbage, then garlic, then ginger, and the lowest number from green onion. Coliforms and E. coli were not detected from all of the raw materials of kimchi. Total aerobic bacteria and lactic acid bacteria of the kimchi showed gradually increasing during ripening and fermentation. The number of psychrotrophilic bacteria showed a similar level in the kimchi. Coliform bacteria were detected at the 3rd, 4th, and 5th day of the kimchi fermentation period, although they were not detected from the raw materials of the kimchi. However, the bacteria were not detected in the kimchi after 6 days. E. coli was not detected in all kimchi samples. The pH value of the kimchi gradually decreased, and acidity increased over fermentation period. This study indicates that there was contamination of coliform bacteria during the process of kimchi preparation, and lactic acid bacteria proliferated in the kimchi during fermentation inhibited the growth of coliforms. More research is needed to evaluate the inhibitory effects of each raw materials of kimchi.

Production of HCN, Weed Control Substance, by Pseudomonas koreensis and its Plant Growth-Promoting and Termiticidal Activities (Pseudomonas koreensis에 의한 잡초제어활성물질인 HCN 생성과 이 균주의 식물성장 촉진 및 흰개미 살충 활성)

  • Yoo, Ji-Yeon;Jang, Eun-Jin;Park, Soo-Yeun;Son, Hong-Joo
    • Journal of Environmental Science International
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    • v.27 no.9
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    • pp.771-780
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    • 2018
  • To develope a microbial weed control agent, HCN-producing bacteria were isolated, and their characteristics were investigated. A selected strain of WA15 was identified as Pseudomonas koreensis by morphological, cultural, biochemical and 16S rRNA gene analyses. The conditions for HCN production was investigated by a One-Variable-at-a-Time (OVT) method. The optimal HCN production conditions were tryptone 1%, glycine 0.06%, NaCl 1%, and an initial pH and temperature of 5.0 and $30^{\circ}C$, respectively. The major component for HCN production was glycine. Under optimal conditions, HCN production was about 3 times higher than that of the basal medium. The WA15 strain had physiological activities, such as indoleacetic acid that was associated with the elongation of plant roots and siderophore and ammonification inhibiting fungal growth, and produced hydrolytic enzymes, such as cellulase, pectinase and lipase. The strain was able to inhibit the growth of phytopathogenic fungi, such as Rhizoctonia solani, Botrytis cinerea and Fusarium oxysporum, by the synergistic action of volatile HCN and diffusible antimicrobial compounds. A microscopic observation of R. solani that was teated with the WA15 strain showed morphological abnormalities of fungal mycelia, which could explain the role of the antimicrobial metabolites that were produced by the WA15 strain. The volatile HCN produced by the WA15 strain was also found to have insecticidal activity against termites. Our results indicate that Pseudomonas koreensis WA15 can be applied as a microbial agent for weed control and also as a termite repellent. Furthermore, it could be applied as a microbial termiticidal agent to replace synthetic insecticides.

Effects of Low Level Laser on the Proliferation and Gene Expression of Fibroblasts and Immune Cells (저출력레이저조사가 섬유아세포와 면역세포의 증식 및 유전자발현에 미치는 영향)

  • Ik-Jun Lim;Keum-Back Shin;Bok Choi
    • Journal of Oral Medicine and Pain
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    • v.20 no.1
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    • pp.53-65
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    • 1995
  • The growth and synthetic activities of fibroblasts are regulated by cytokines and growth factors derived from activated inflammatory cells. Stimulatory effect of low level laser (LLL) radiation on wound healing seems to be in part due to direct stimulatory action on cell proliferation and synthetic activities of fibroblasts. Also indirect stimulatory effect on the fibroblast function through inflammatory or immune cells is another possible mechanism of biostimulatory action of LLL. This study was performed to determine the growth rate of human gingival fibroblasts obtained biopsy and culture, fibroblast cell line, and immune cell line by using $[^3H]-$ thymidine incorporation test. And gene expression pattern was also analyzed by using the DNA probe such as Hsp70, IL-1$\beta$, MIP-1$\alpha$ and actin cDNA. Proliferation rate of gingival fibroblast was increased by LLL irradiation, but no more effect was added by LPS or IL-1$\beta$ pretreatment Enhanced Hsp70 gene expression was found from gingival fibroblasts and fibroblast cell line COS by LLL irradiation., which was not more increased by LPS or IL-1$\beta$ pretreatment. LLL-irradiated promyelcytic cell line HL-60 and macrophage cell line RAW264.7 showed significant stimulatory effect of proliferation rate when compared with respective control. However there were no changes in growth rate of other immune cell tested in this study, such as B cell line WR19n.l and 230, helper T cell line Jurkat and Hut78, cytolytic T cell line CTLL-r8. By LLL-irradiation Hsp70 gene expression was increased in RAW246.7 and HL-60, not in CTLL-R8. And IL-1$\beta$ and MIP-1$\alpha$ gene expression were induced only from LLL-irradiated RAW264.7. These results led us to presume that LLL radiation may affect to the immune cells, especially to macrophage, through which it might promote wound healing process.

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An Experimental Study on the Combustion Characteristics in Low Emission Multi-Staged Oil Burner (다단연소를 이용한 저 NOx 버너의 연소특성에 관한 연구)

  • An, Guk-Yeong;Kim, Han-Seok;Jo, Eun-Seong
    • 연구논문집
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    • s.27
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    • pp.101-108
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    • 1997
  • The characteristics of combustion and emissions in multi-staged oil burner have been experimentally studied for the various range of equivalence ratios, drop sizes and fuel formulations. Malvern system was used to measure droplet size of fuel. Light fuel oil and light fuel oil doped with pyridine($C_5H _5N$) were used to investigate the effects on fuel NOx emission. The emissions of NO and CO in exhaust gas and the flame temperatures were measured by the gas analyzer and thennocouples. NOx emissions were increased by increasing the excess air ratio (range:$lambda=1.1-1.4$) or decreasing the SMD of droplet in single-staged burner. In comparison with the single-staged burner, the emission of NOx in multi-staged burner was reduced by 50% but CO emission was slightly increased. It is found that multi-staged burner has a good capability in reducing thermal NOx resulting from the distributed heat release rate and lower flame temperature in fuel-rich and fuel-lean combustion zone. Moreover, the fuel NOx emission of the multi-staged burner is lower than that of single-staged burner, because multi-staged burner has fuel rich zone where fuel N is converted to $N_2$ more than NO. In 3-staged burner, the percentage of each stage combustion air have strong influence on emission characteristics. It is also found that NOx emission can be reduced by decreasing inner and outer air percentage or increasing middle air flow rate and CO emission is vice versa.

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