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An Evaluation of Smeared Zone Due to Mandrel Penetration (맨드렐 관입에 기인하는 스미어 존의 평가)

  • 박영목
    • Journal of the Korean Geotechnical Society
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    • v.19 no.6
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    • pp.217-225
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    • 2003
  • An experimental study was conducted to evaluate the smeared effect due to mandrel penetration into soft ground for a vertical drain installation. Laboratory tests were performed to investigate the formation of a smear zone, the variations of strength, and the consolidation characteristics in the disturbed zone using two types(CL at Yangsan site and OH at Pohang site) of soft clayey soils. The smear zone effect was evaluated focusing on mandrel shape, mandrel size, penetration speed, and ground condition. Based on laboratory test results, the diameter of the smear zone$(d_s)$ ranged from 3.08 and 3.92 times that of mandrel$(d_m)$. It was also found that the $(d_s/d_m)$ value of the circular shape of the mandrel is smaller than those of square and rectangular shapes. The value of $(d_s/d_m)$ decreased with larger mandrel size, lower penetration speed in the CL soil, and higher penetration speed in the OH soil. However, natural water content was minimally affected by $(d_s/d_m)$. Respectively, the coefficients of horizontal consolidation$(C_{hs})$ and horizontal Permeability$(K_s)$ of smear zone ranged from 0.81 to 0.87 times, and 0.73 to 0.83 times those of the undisturbed zone. Based on this study, the values of $C_{hs}, K_s$ and unconfined compressive strength$(q_{us})$ in the smear zone were the lowest at close vicinity of the mandrel and increased linearly with distance from the mandrel. Further, the $(q_{us})$ varied from 0.5 to 0.9 times that of the undisturbed zone strength.

Molecular Characterization of Metallothionein Gene of the Korean Bitterling Acheilognathus signifer (Cyprinidae) (묵납자루 (Acheilognathus signifer; Cyprinidae) metallothionein 유전자의 클로닝 및 특징 분석)

  • Lee, Sang-Yoon;Bang, In-Chul;Nam, Yoon-Kwon
    • Korean Journal of Ichthyology
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    • v.23 no.1
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    • pp.10-20
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    • 2011
  • Genetic determinant for metallothionein (MT), a cysteine-rich protein playing essential roles in metal detoxification and homeostasis, was characterized in the Korean bitterling (Acheilognathus signifer, Cyprinidae), an endemic fish species. The full-length A. signifer MT (AsMT) cDNA (551 bp) is composed of a single open-reading frame (ORF) to encode a polypeptide of 60 amino acids containing 20 cysteine residues whose positions are conserved in most cypriniform MTs. At the genomic level, the AsMT (2,593 bp spanning the 5'-flanking region to the 3'-untranslated region) represented a conserved tripartite (three exons interrupted by two introns) structure with AT-rich introns. The upstream regulatory region (-1,914 bp from the ATG initiation codon) of AsMT displayed various sites and motifs for transcription factors involved in the metal-mediated regulation and stress/immune responses. The AsMT transcript was ubiquitously detected in various organs with variable expression levels, where the ovary and intestine showed the highest expression, while the heart and skeletal muscle represented the lowest level. During an exposure to copper (immersion in $0.5\;{\mu}M$ Cu for 48 h), the levels of AsMT transcripts were significantly elevated in the liver (more than 3.5-fold), moderately in the gill, kidney, and spleen (ranging from 1.5- to 2.5-fold), and barely in the brain and intestine. Results of this study could form a useful basis to explore the metal-related stress physiology of this endangered fish species.

PARAMYOSIN OF THE ABALONE, NOTOHALIOTIS DISCUS (전복 Paramyosin의 분리 및 그 성질)

  • PYEUN Jae Hyeung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.5 no.1
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    • pp.29-38
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    • 1972
  • The protein composition of abalone muscle was estimated with the following result: on a series of samples analyzed, water-soluble protein, $19\~22\%$, salt-soluble protein, $27\~39\%$: alkali-soluble protein, $20\~26\%$ : and stroma $20\~28\%$ : respectively. It was demonstrated by ultracentrifugal analysis that approximately $65\%$ of the salt-soluble protein is accounted for by paramyosin, $30\%$ by actomyosin, and $5\%$ by myosin, respectively. The ultracentrifugally homogenous paramyosin was prepared by BAILEY's ethanol-dried method. It showed a $S^{\circ}\;_{20,\;{\omega}$ of 3.14s, and was completely salted in with KCl beyond $0.35{\mu}$. The intrinsic viscosity at $25^{\circ}C$ was estimated at 3.1. The paramyosin is rich in several amino acids such as arginine, aspartic acid, glutamic acid, etc., and lacking of both proline and tryptophane, in rough accord with other paramyosins reported. The abalone paramyosin did not show ATPase activity over a pH range of 5 to 9,5 even in the presence of Ca++ or Mg++. So was the case with the paramyosin specimen prepared by BAILEY's wet-extraction method.

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Management of Fast Putting Green by Using Green Speed Expectation Models (그린 스피드 예측 모형을 통한 빠른 그린 관리 방법)

  • Jang, You-Bee;Shim, Kyung-Ku
    • Asian Journal of Turfgrass Science
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    • v.20 no.1
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    • pp.11-23
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    • 2006
  • This study was carried out to propose low types of green speed expectation models for fast putting green management by changing mowing height($4.0{\sim}2.5$ mm) and timing of rolling, dew removal and dew removal+rolling. Ball roll distance data were taken from the creeping bentgrass(Agrostis palustris Huds. 'Penncross') practice green of east course at the Lakeside C.C. in October 18, 2001 and May 25, 2002. Data were subjected to multi-regression analysis using Statistical Package for the Social Science. Among four types of green speed expectation models, the best multiple-regression equation for fast green management was as follows; $Y_4=4.171-0.225{\cdot}X_1-0.038{\cdot}X_2$ (where, $Y_4$ : green speed(m) after single dew removal+single rolling, $X_1$ : mowing height($4.0{\sim}2.5,\;X_2$ : passage of time ($0{\sim}8$ h.)). The equation[single dew removal by using sponge roller $\rightarrow$ single mowing at 3.0 mm height or less $\rightarrow$ single rolling] explained to provide fast green over 3.2 m (Stimpmeter readings required for USGA championship play) until the end of first round. Therefore, this cultural practice system was believed to provide fast putting green condition for professional golf tournament

Mechanical reliability of Sn-37Pb BGA solder joints with high-speed shear test (고속전단 시험을 이용한 Sn-37Pb BGA solder joints의 기계적 신뢰성 특성 평가)

  • Jang, Jin-Kyu;Ha, Sang-Su;Ha, Sang-Ok;Lee, Jong-Gun;Moon, Jung-Tak;Park, Jai-Hyun;Seo, Won-Chan;Jung, Seung-Boo
    • Journal of the Microelectronics and Packaging Society
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    • v.15 no.4
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    • pp.65-70
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    • 2008
  • The mechanical shear strength of BGA(Ball Grid Array) solder joints under high impact loading was investigated. The Sn-37Pb solder balls with a diameter of $500{\mu}m$ were placed on the pads of FR-4 substrates with ENIG(Electroless Nickel Immersion Gold) surface treatment and reflowed. For the High Temperature Storage(HTS) test, the samples were aged a constant testing temperature of $120^{\circ}C$ for up to 250h. After the HTS test, high speed shear tests with various shear speed of 0.01, 0.1, 1, 3 m/s were conducted. $Ni_3Sn_4$ intermetallic compound(IMC) layer was observed at the solder/Ni-P interface and thickness of IMC was increased with aging process. The shear strength increased with increasing shear speed. The fracture surfaces of solder joints showed various fracture modes dependent on shear speed and aging time. Fracture mode was changed from ductile fracture to brittle fracture with increasing shear speed.

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Studies on Anion, Element, Chromaticity and Antioxidant Activities of Commercial Dried Lavers (Porphyra yezoensis) Cultivated in Korea (시판 건조김의 음이온, 원소, 색도 및 항산화 활성)

  • Park, Wook-Min;Kim, Kee-Woong;Kang, Dong-Soo;Bae, Tae-Jin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.2
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    • pp.323-327
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    • 2014
  • This study was carried out to provide basic data on the anion, element, chromatic and antioxidant activities of commercial dried lavers cultivated from Seocheon, Wando, Goheung and Busan in Korea. The highest concentrations of several anions ($Cl^-$, $NO_3{^-}$, $SO_4{^2-}$, and $PO_4{^3-}$ per 100 g of dried laver) were 412.00 mg in Wando, 545.90 mg in Goheung, 297.35 mg in Seocheon, and 79.70 mg in Wando, respectively. The highest concentrations of N, C, and S were 6.40%, 41.36%, and 0.98%, respectively, in dried laver cultivated from Wando. Hunter's color values were significantly high in the order of Seocheon < Goheung < Busan < Wando for lightness, Wando < Busan < Goheung < Seocheon for redness, and Goheung < Seocheon < Busan < Wando for yellowness in the dried lavers. Total phenolic content and DPPH (1,1-diphenyl-2-picryl-hydrazyl) radical scavenging activities showed highest values of 166.10 mg/100 g and 28.48%, respectively, in dried laver cultivated from Wando.

Manufacture of Dropwort Extract Using Brown Sugar, Fructose Syrup and Oligosaccharides (흑설탕, 과당, 올리고당을 이용한 미나리 추출물의 제품화)

  • Son, Min-Jung;Cha, Chun-Geun;Park, Jung-Hyun;Kim, Chan-Shick;Lee, Sam-Pin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.9
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    • pp.1485-1489
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    • 2005
  • The dropwort was fermented by steeping with brown sugar, fructose syrup or oligosaccharide at room temperature for 2 month, and then stored at cold room for 6 months. The dropwort extracts prepared with three different sugars showed more than $50^{\circ}$Brix, below pH 4.0 and about $0.7\%$ titratable acidity. The dropwort extract with brown sugar showed $1.6{\times}10^6$ viable cell counts and $21.2\%$ reducing sugar. Formation of $CO_2$ gas was superior to the dropwort fermented with brown sugar or oligosaccharide. The dropwort extract with fructose syrup indicated $9.0{\times}10^3$ viable cell counts and $50.1\%$ reducing sugar. Microorganism present in fermented dropwort extract was effectively pasteurized by the addition of $3\%$ citric acid and heat-treatment at $85^{\circ}C$ for 15 min, resulting in the less production of $CO_2$ gas. The dropwort extracts prepared with brownsugar, fructose syrup or oligosaccharide was suitable for the standardization that required for plant extract in Korea Food Codex.

Uncertainty evaluation for the determination of creatinine in urine by LC-MS/MS (LC-MS/MS를 이용한 소변 중 크레아티닌 분석의 측정불확도 평가)

  • Kim, Jin-Young;Kwon, Woon-Yong;Suh, Sung-Ill;In, Moon-Kyo
    • Analytical Science and Technology
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    • v.25 no.1
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    • pp.83-90
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    • 2012
  • The objective of the study was to estimate the measurement uncertainty associated with determination of creatinine (Cr) in urine samples by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Centrifuged urine samples (10 ${\mu}L$) were diluted with 390 ${\mu}L$ of distilled water. To 20 ${\mu}L$ aliquots of diluted urine samples, 30 ${\mu}L$ of internal standard solution (Cr-$d_3$, 5 ${\mu}g/mL$) and 10 ${\mu}L$ of acetonitrile were added and filtered. The samples (1 ${\mu}L$) were introduced into LC-MS/MS with no further pretreatment. Cr was separated on a multi-mode ODS column (Scherzo SM-C18, 75 ${\times}$ 2.0 mm I.D., 3 ${\mu}m$) and quantified by LC-MS/MS operating in MRM mode (Cr, m/z 114.0${\rightarrow}$ 86.0; Cr-$d_3$, m/z 117.0${\rightarrow}$ 89.1). The four factors that contribute uncertainty to the final result were extracted and evaluated. The principal factors of contribution to combined standard uncertainty were sample dilution, calibration curve and repeatability, while the preparation of standard solution was only a minor factor. Relative extended uncertainty of the measured concentration was 14.2% in a real urine sample.

Coupled Thermal-Hydrological-Mechanical Behavior of Rock Mass Surrounding Cavern Thermal Energy Storage (암반공동 열에너지저장소 주변 암반의 열-수리-역학적 연계거동 분석)

  • Park, Jung-Wook;Rutqvist, Jonny;Ryu, Dongwoo;Synn, Joong-Ho;Park, Eui-Seob
    • Tunnel and Underground Space
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    • v.25 no.2
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    • pp.155-167
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    • 2015
  • The thermal-hydrological-mechanical (T-H-M) behavior of rock mass surrounding a high-temperature cavern thermal energy storage (CTES) operated for a period of 30 years has been investigated by TOUGH2-FLAC3D simulator. As a fundamental study for the development of prediction and control technologies for the environmental change and rock mass behavior associated with CTES, the key concerns were focused on the hydrological-thermal multiphase flow and the consequential mechanical behavior of the surrounding rock mass, where the insulator performance was not taken into account. In the present study, we considered a large-scale cylindrical cavern at shallow depth storing thermal energy of $350^{\circ}C$. The numerical results showed that the dominant heat transfer mechanism was the conduction in rock mass, and the mechanical behavior of rock mass was influenced by thermal factor (heat) more than hydrological factor (pressure). The effective stress redistribution, displacement and surface uplift caused by heating of rock and boiling of ground-water were discussed, and the potential of shear failure was quantitatively examined. Thermal expansion of rock mass led to the ground-surface uplift on the order of a few centimeters and the development of tensile stress above the storage cavern, increasing the potential of shear failure.

A Bioassay Method with Radish Seedlings for Bioactive Substances (무우 유묘(幼苗)를 이용(利用)한 식물생리활성물질(植物生理活性物質)의 생물검정법(生物檢定法))

  • Hong, K.S.;Lim, H.K.;Cho, K.Y.
    • Korean Journal of Weed Science
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    • v.8 no.3
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    • pp.244-249
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    • 1988
  • Some experiments were conducted to utilize the raphanus test as a bioassay method for plant growth regulaters and hormonal herbicides. Among seven radish cultivars, the response of Jangbaegminong was the most sensitive and quantitative to ${\alpha}$-NAA and its coefficient of variation value was the lowest. The optimum application time of test compounds was estimated seven to nine days after seeding. After ${\alpha}$-NAA treatment the curvature angle of radish cotyledonary petiols was sharply increased to the maximum value at 24 hours after and was changed by light and temperature conditions. The curvature response was observed by application of ${\alpha}$-NAA, phenoxy and indole compounds but often plant growth regulators, $GA_3$ benzyladenine, ABA and homobrassinolide was not detected by this method. So this bioassay was useful to evaluate the activity of a compound as an auxin or a hormonal herbicide.

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